Brief Biography Dr. Aminah Ali Abid Al-juboori, is a Lecturer at the University of Baghdad/College of Pharmacy. Originally, she obtained a Bachelor and Master of Clinical Biochemistry from University of Baghdad/College of Pharmacy. After that she joined the Global Centre for Gynaecological Diseases at University of Newcastle in Australia to get PhD degree in Medical Biochemistry (2021). Her work focused on the translational research through utilising the cutting-edge techniques of Omics technologies and bioinformatic analysis on human in vitro model to enhance infertility treatment for endometriosis women particularly those desiring family. Her expertise in advanced cell culture modelling system is evident through landmark paper in Proteomics journal, where she established a unique three-dimensional (3D) co-culture model of stromal and epithelial cell communications in human endometrial cancer. Now, she is passionate to develop patient-derived organoids that promised to get biomarkers and medicines discovery approved and into hands of the patients who need them much sooner for more personalised treatment approaches. By way of this her goal is to improve treatment response, survival, and quality of life for various diseases and cancers patients.
Cell Culture (Mammalian cell line) (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Paraffin sectioning of tissue and cell culture spheroids samples (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Immunofluorescence, Immunohistochemistry (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Fluorescence and confocal microscopy (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Clinical sample collection (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Patient sample collection and processing from surgical theatre (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Primary Cell Culture 3 dimensional (3D) and 2 dimensional (2D) (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Handling of epMotion machine and software for drug screening on 3D co-culture spheroids (2016), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Transcriptomics technique (2017), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Proteomics technique (2017), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Secretomics technique (2017), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Metabolomics technique (2018), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Lipidomics technique (2018), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Computational Biology (proteins prediction and Pathway analysis) (2018), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Fluorescence-activated cell sorting (FACS) and Flow cytometry (2019), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Polyacrylamide Gel Electrophoresis (SDS-PAGE & RNA-PAGE) (2019), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Real Time PCR (Relative Quantitation) (2019), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Handling of bead based Multiplex ELISA (LUMINEX) (2019), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Measuring many medical biomarkers using spectrophotometry and ELIZA techniques (2019), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Different nucleic acid extraction procedure (2020), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Different molecular biological techniques (2020), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Different immunological techniques (2020), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
Animal work (2021), School of Biomedical Science and Pharmacy, University of Newcastle, Australia
PCR and RT-PCR technologies (2023), training course at Biotechnology Research Centre, Al-Nahrain University
Iraqi Tuition Fees Scholarship for PhD Research in the University of Newcastle (2015)
Beautiful Science Award (2018) from School of Biomedical Science and Pharmacy, University of Newcastle, Australia Gratitude and Appreciation Letters of Iraqi Cultural Attaché in Canberra, Australia (2021)
I am interested in understanding the development of various gynecological and obstetric diseases to improve the women health and ultimately their quality of life. In my master’s research, I detected some predictive biomarkers for pregnant women suffering from polycystic ovary syndrome (published in AJPS, 2012). The master’s research work was done in a well-reputed hospital research centre in Iraq named Kamal Al Samaria hospital that provides assisted reproductive technology and in vitro fertilisation (IVF) for infertile women. Based on my master’s research findings, the hospital administration considered screening my predictive biomarkers as condition for nominating women for IVF cycle. After that I complete my PhD research in the Global Centre specialized in gynecological diseases at University of Newcastle in Australia to get the PhD degree in Medical Biochemistry. For the first time, I have established laboratory models mimic the reproductive diseases. These models represent millions of mini-organ like structures (scientifically known as co-culture organoids), that comprised of all cell types presence in a complete tissue and they reflect the exact functional complexity of biological tissues. My recent publication in a high esteemed journal named “Proteomics”, has displayed the robustness of my model that be used to study endometrial cancer, where it can exactly mimic the patient endometrial cancer tissue in regard to morphology and protein expression. These models aid us in deciphering the molecular mechanisms underlying various reproductive pathologies helping us to discover novel diagnostic markers and targeting therapies. Moreover, this invention will serve in favour of public interest by saving a huge expenditure on multiple attempts for a successful medicament and cure of different gynecological pathologies.
Clinical biochemistry, Advanced cell culture techniques, Omics technologies, Endometrial cancer, Endometriosis, Recurrent miscarriages, Failed embryo implantation
Biochemistry
Epithelial and stromal communications are essential for normal uterine functions and their dysregulation contributes to the pathogenesis of many diseases including infertility, endometriosis, and cancer. Although many studies have highlighted the advantages of culturing cells in 3D compared to the conventional 2D culture system, one of the major limitations of these systems is the lack of incorporation of cells from non‐epithelial lineages. In an effort to develop a culture system incorporating both stromal and epithelial cells, 3D endometrial cancer spheroids are developed by co‐culturing endometrial stromal cells with cancerous epithelial cells. The spheroids developed by this method are phenot
The objective of this study is to determine levels of some pro-inflammatory cytokines (IL-12 and SIL-2R) in pregnant women with polycystic ovary syndrome (PCOS) and also to determine incidence of spontaneous abortion during first trimester in pregnant women with PCOS compared with non-PCOS pregnant women. This Study was performed on (93) women, consisted of: 1- (63) pregnant women during the first trimester, which included:A- (33)PCOS pregnant women and subdivided into the following groups: (20) successful pregnant PCOS, (7) blighted Ovum PCOS and (6) missed abortion PCOS. B- (30) non-PCOS pregnant women, which included: (27) successful pregnant control and (3) non-PCOS pregnant women were withdrawn from this study because of their abortion
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