This study was conducted to investigate the inhibition role of alcoholic extracts of plants Trigonella foenumgraecum , Piper cubeba , Piper nigrum, Camphora , Commiphora molmol, Apium graveolens , Arachis hypogaea , Linum usitatissumun , Cuminum cyminum , Pimpinella anisum , Lepidium sativum , alcoholic extracts of plants used was prepared and used of concentration 200 mg/ml and add 0.2 ml of each extracts to 10 ml of media and 0.2 ml of Ethambutol to 10 ml of media the drug for mycobacteria and media with out any adding for controls. Cultural media were inoculated with 3 drops of bacterial suspension ( 1mg /ml ) in concentration 108 cfu/ml from patient suffering from tuberculosis and after incubation on 37 °C the result read weekly up

Back ground: Toxoplasmosis is an important zoonosis that causes economic losses in animal herds due to abortion and stillbirth as well as changes in the reproductive and neural system of susceptible animals . Objective: The aims of the present study is to determination the prevalence of T. gondii in farm animals ( sheep& goat)of both genders and different ages in Sulaimani province by using two serological tests (ELISA and LAT). Methods: Blood samples were collected from farm animals ,142 sheep and 46 goats , of different sexes and ages. Tow different serological tests ,ELISA and LAT for qualitative determination of T. gondii antibody titer in sheep and goats serum samples. Results: The prevalence rate in sheep was 73 (51.7 %) and 82

The purpose of the study was to determine the efficacy and safety of
intratesticular injection of ethanol or formalin as a novel method for
chemosterilization of rabbits. Twenty rabbits, aged (6-8 months) and weighed
(1.3-1.5) kg were used. They were randomly divided into two equal groups. In
the first group, absolute ethanol and in the second group, formalin1% at a dose
of 1ml/testis for each agent were injected under the effect of xylazine, by
insertion of the needle from the caudo-ventral aspect of the testis then pushed in
the center of the testis toward the cranio-dorsal pole of the testis. Orchidectomy
was done at first and second months postinjection, samples from the testes and
epididymis were taken for

Branchiomycosis is a fungal disease that infects fish gills. It was
identified by isolation and histopathological changes of examined gills in
common carp fish (Cyprinus carpio) which, were obtained from fish farm in
Duhok Governorate, Iraq. The infected fish were suffering from respiratory
disorders; gulping air at the water surface, rapid movement of operculum and
massive mortality, which resulted in the loss of 95% of fish pond. The gills
appear marbled appearance with necrotic areas on the localized damage gills.
The causal pathogen was identified as Branchiomyces sanguinis, in which the
diameter of spores and non-septated hyphae are 5-7 μm and 12 – 20 μm,
respectively. In histopathological preparation,

The current study conducted in the Duhok province villages, Kurdistan region of Iraq during the period between September-December 2008, fulfilled by using Rosebengal and ELISA tests cattle of different sex and age were used comparing between these two tests and to determine the prevalence rate by both tests. A total number of 360 serum samples were collected (294 females, 66 males). The infected rate by RBT was 6.38% and by ELISA were 0.83%. According to the different animal ages and sex group showed that the infection rates were 5.4% and 12.1% in females and males, respectively. Cattle in age group 1-3 years and more than 4 years were showed that an infection rates were 3.8% and 11.8% respectively.

Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid products placed between two electrodes. So this study was conducted for the quantitative assessments of mild PEF conditions on acid tolerance, growth, bile tolerance and protease activity of Lactobacillus delbrueckii ssp. bulgaricus LB-12. The control and PEF treated samples were prepared by inoculating 10 ml of freshly thawed culture of Lactobacillus delbrueckii ssp. bulgaricus LB-12 into 990 ml of sterile 0.1% (wt/v) peptone water and treated in a pilot plant PEF system (OSU-4M). The treatments were positive square unipolar pulse width of 3 μs, pulse period of 0.5 second, voltage of 1 kV/cm, delay time of 20 μs and f

The purpose of this study was to evaluate the effect of different toxic doses
with regard to toxicopathological changes in mice injected with ethanolic crude
extract of Euphorbia granulata F. The experiment was done on (24) mice of
(6-8weeks age and 20-25g weight ) divided equally into (4) groups as follows:-
First group injected distilled water subcutaneously for 45 days and considered
as control ,second group injected subcutaneously (600 mg/kg B.W/day)
ethanolic extract of E. granulata for 45 days, Third group injected
subcutaneously (800 mg/kg B.W/day) ethanolic extract of E. granulata for 45
days. Fourth group injected subcutaneously (1000 mg/kg B.W/day) ethanolic
extract of E. granulata for 45 days.The mic

Magnetic therapy was applied in the present study, which play two roles; as antimicrobial (Escherichia coli, Staphylococcus auras, Streptococcus spp, Klebsialla ,and Pseudomona ) and as stimulator to tissue repair.24 rabbits were used in this study, they divided into two groups; 1st group(treated group) contains 20 rabbits, 2nd group (control group) contains 4 rabbits, the last subdivided into two subgroup (standard control subgroup and sham-control subgroup, the left side of all animals in control group is standard control subgroup, while the left side is sham-control subgroup). After surgery to all, the wounds were exposed to pulsed magnetic field except standard control subgroup, they healed with conventional treatment, 1st group(trea

The aim of this study is to evaluate the use of fimbriae antigens for immunization of rabbits against Proteus vulgaris bacteria results showed a higher significant difference (P<0.05) in erythema diameters in the immunized groups in compared with the control. There was no significant difference between both immunized groups 200 μg/ml and 100 μg/ml after 24 hours. Also, a higher significant differences (P<0.05) in the diameter of induration is recorded in both immunized groups compared to control site, a higher significant differences (P<0.05) in the immunized group (100 μg/ml) at the concentration 200 μg/ml compared with 100 and 50 μg/ml after 24 hours, as follow as after 48 hours except significant differences between 200

In order to study the vaginal defensive mechanisms in ewes, vaginal smears and biopsies were collected from eight adult Awasi ewes. The biopsies samples were processed by the routine histological methods and stained by H & E and PAS stains, while the smears samples were stained with MB. Samples were examined under light microscope. The resent study revealed that the vaginal wall lacks many important constituents, among these were the vaginal glands, goblet cells, muscularis mucosa and lymphatic nodules. And as the vagina was the nearest organ to the external environment and as it receives the male copulatory organ, so it is more liable organ to be infected by external pathogens. On the contrary, the vagina has special compensatory hi

This trail was suggested to evaluate the amount of inherited haemagglutination inhibition (HI) antibodies for Newcastle Disease (ND) from hens to their own progeny via yolk, blood samples were collected from broiler breeders at 51st week of age aiming the collection their sera, these hens were previously vaccinated with ND-killed vaccine at the age 5 and 120 days respectively via subcutaneous route; and ND-alive vaccine at 1and 18 days then monthly intervals by aerosol, random samples of eggs were collected from panels or hatchery machines either after 24h., to detect amount and location of HI antibodies through them, day old chicks were submitted to the same protocol of blood collection as well as mothers. The results showed that the co

This study was undertaken to prepare Heat Killed Candida albicans Antigen (HK-CA) and to investigate it's effect on elicitation of Delayed-type Hypersensitivity (DTH) and protection from virulent Candida challenged in mice, and described the major effect of Nocardia on the immunogenicity of this Ag.Four groups of 6 males Swiss mice for each group were immunized intraperitonialy(I.p)for 10 days at 5 doses(one day intervals) with 1x108 HK-CA plus Nocardia cell sap as adjuvant(CFA),the second group immunized with HK-CA plus Freund incomplete adjuvant,the third group immunized with HK-CA only and the fourth group treated with normal saline. Another group of 6 animals was immunized intranasally(I.n)with HK-CA only with the same doses as previ

During the period from November 2007 till October 2008, a total of 255 specimens of the cyprinid fish, from freshwater fish Cyprinus carpio Linnaeus, 1758, were collected from fish markets east of Baghdad city. Microscopic examination of these fishes revealed that they were infected with two species of the crustaceans ]Argulus foliaceus (0.8%) and Ergasilus sieboldi (20%)[.The present study included the following: The clinical signs which were caused by the crustaceans, which included redness and an opacity of the skin, increase of mucus secretion and rapid movements of the operculum. The percentage incidence of the infection with these parasites were also found to be related to different length groups of the host, the larger fishes were

In order to study the pathogenesis of the Pasteurella multocida in its natural rout of infection, this study was done to investigate and compare its pathogenesis after intranasal and intraocular infection.Thirty (30) rabbits were divided into three groups, the 1st group (n=12) infected with 240 CFU of Pasteurella multocida intra-nasal, the 2nd (n=12) infected with 240 CFU of P. multocida intra-ocular (eye drop), and the 3rd group (n=6) served as control negative. The animals were sacrificed if they did not die naturally at 24-48hr, 3, 6, 9, 30 day post-infection (p.i.). The results showed dullness of the animals with nasal and ocular discharge and some animals dead at 48hr and 72 hr p.i. (two animals from the 1st group and four animals f

gainst damage initiated by a variety of endogenous and exogenous compounds. This study was conducted to evaluate the influence of Benzo[α]Pyrene (BαP) administration on the liver antioxidant defense system. Chicks were assigned into five equal groups, as control, tricaprylin group and three groups treated with BαP (1.5 μg, 150 μg or 15 mg/kg BW). Five birds were sacrificed at days 7, 14, 21 and 35 from each group. To assess the liver antioxidant defense system, glutathione (GSH) concentration, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities were employed. It was found that 15 mg of BαP level create significant increase (P<0.05) in the GSH level, GSH-Px, SOD and CAT activities (18.483, 9.

The current study was conducted for the measurement of normal range and mean value of serum alanine aminotransferase (ALT) , aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in (125) clinically normal Iraqi racing horses (males and females , Arabian and Crossbred) , aged 2 – 8 years ; < 4 , 4 and > 4 age groups . The results revealed that the range and the mean value ± SEM of these enzymes were as follows ; serum ALT 2 – 28 U/L and 11.07 ± 0.62 U/L, serum AST 30 – 210 U/L and 78.00 ± 3.62 U/L and serum ALP 63 – 204 IU/L and 96.84 ± 2.52 IU/L respectively .However, significant difference (P < 0.05) in serum ALT was recorded between Arabian and Crossbred , while serum AST values showed a significant diff

Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid products placed between two electrodes.  Streptococcus thermophilus is an important bacterium used for the production of fermented dairy products.  Objective of this study was to determine the influence of a mild PEF condition on the growth and protease activity of Streptococcus thermophilus.  A range of mild pulsed electric field conditions were earlier screened by the author to arrive at an optimum overall mild pulsed electric field condition for various probiotic characteristics.  Freshly thawed Streptococcus thermophilus was suspended in 0.1% w/v sterile peptone water an

This study presents the effects of Date Palm Pollen grains (DPP) (phoenix ductylifera) on testes function, fertility and pregnancy index in male adult rats.Forty adult male albino rats were divided randomly into four equal groups .The first group  was not received DPP and considered as control group and the other groups (T1, T2 and T3) were received daily gavages of aqueous suspension(DPP) containing 30, 60 and 120 mg/kg B.W for 56 days. At the end of experiment blood were collected to determine serum testosterone level. Sexual reaction was determined and reproductive indices (fertility, pregnancy) index were examined in normal fertile 32 female rats mated with treated male rats. Sperm concentration, mortality and

This study designed to know the effect of use different ration from mixing between minced meat With fat on total bacterial count of meat ( 80) sample was examine through the period of this study (40 sample for minced meat and 40 sample for minced fat) collected from shops in Baghdad city. The results showed the means of bacterial count was rise for the group contained just minced meat without any mixing in means reach to 9.6x 10 cfu \ml follow it with second group which include only minced fat by count 5.5x10 cfu\ml, and the study showed also decrease in bacterial count for (3) mixing groups ( fat with meat ), so the bacteria count for the third group which included 5%, from fat 1.1x10 cfu\ml and 1.3x10 for each of fourth and fifth group

The object of this study was to evaluate coating table eggs with different medicinal oils and their effects on shelf life properties of fresh eggs. After storage for 1, 2, 4 and 8 weeks at room temperature (experiment 1) and refrigerator temperature (experiment 2) . Fresh table eggs were collected from a flock of Isa brown laying hens, the eggs were distributed into four groups as follow :-1-Control group 2-Corn oil group 3-Dill oil group 4-Black seed oil group, the eggs were coated by immersioned in oil at 56.7 C for16 min (Oil thermostabilization) . The results revealed that coating table eggs with oils in general and specially medicinal oils reduced the mean decrease in egg weight , egg weight loss , PH , free fatty acids , total bact

Two hundred and four Broiler chicks was used, divided into six equal groups, each group was vaccinated with one vaccine as fellows: BUR-706 group1 (G1), IBD-L (G2), TAD (G3), CH/80 (G4), D78 (G5) and Control (G6). ELISA test was used to estimate ND and Gumboro disease antibodies. Aspartate amino transferase (AST), Alanin amino transferase (ALT), Alkaline phosphatase (ALP) activities, total protein and albumin and globuline concentrations in blood serum were estimated. Newcastle disease antibodies titers were high in G1 but low in G5 significantly. Gumboro disease titers were nonsignificant increase(p>0.05) in G1, G2 and G3 but nonsignificant low(p>0.05) in G4 and G5. Normal total protein, albumin and globuline concentrations and no

This study was conducted to investigate the adverse effect of aflatoxine contaminated ration on immune response induced by N D vaccine in broilen and the effect of adding vit C in drinking water in reducing the aflatoxine immune supportive effect.
A total of 75 day old broiler chicken were divided randomly in to three equal groups , the first group was feed aflatoxine free ration while second and third groups were feed on aflatoxine contaminated ration at 6 day of age, in addition to that the third group have received vit C in a drinking water (125 mg/litter). The birds were immunized twice with ND vaccine at 10, 20 day of age and ELISA test was carried out to estimate the humeral immunity response at 6, 19, 31, days of age.
All th

Three types of Nerium.oleander leaves of hexane extract were prepared according the color of their flower, red flower group (RFG), pink flower group (PFG) and white flower group (WFG).After drying in the sunlight, grinding by electrical grinder. Extraction with hexane was done by a soxhlet apparatus for each type the extract of each type was dissolved in propylene glycol which was used to dissolve the extract with the aid of magnetic stirrer mixer for ten minutes. The median lethal dose (LD50) experiment for each type, fifty adult mice of mixed sex were used. They were divided into 5 equal number groups and were given different oral doses as following:-The red flower groups (RFG) received oral doses ranging from 225-425mg⁄Kg ,the pink

The study was conducted to indicate the compartive measurements of heavy metals residues levels were detected by Atomic Absorption Spectrophotometer in 10 random samples of locally processed cheeses and in 20 random samples of emulsifying salts collectd from different areas in Baghdad , results showed that such cheese of low quality and did not meet both local and international Dairy products standards , Such findings of high concentration of heavy metals residues indicate that the emulsifying salts used in processing were not nutritional but of industrial origin and unfit for human consumption . The results show that is the concentration *[p.p.m.] of heavy metals residues **(±MD) in the locally processed cheeses samples were < 0.1±

This study was carried out to evaluate seven types of herbs being: black pepper seeds (Piper nigrum), celery seeds (Apium graveolens), cubeb seeds (Piper cubeba), camphor seeds (Cinnamomuum camphora), myrrh (Commiphora mol mol), fenugreek seeds (Trigonella foenum- graecum) and seeds of Lepidium sativum. The antibacterial activity of alcoholic extracts of these plants was performed on gram- positive and gram- negative bacteria isolated from human respiratory tract infections including : S. aureus ,S. pneumoniae, M. antarcticus, Corynebacterium spp. and coagulase- negative Staphylococci (CNS). Gram –negative bacteria included: K. oxytoca, K. pneumoniae and N. meningitidis, P. aeruginosa and E. coli. We studied the bacterial sensitivity t

40 Mmermrij fish Mastacembelus mastacebelus were collected from different areas in Baghdad , a total of 20 fish from each area; the first 20 from area near a Al Muthanna bridge, and the second from the old Diyala bridge. stomach contents Were taken from these fish and analyzed by the Gel electrophoresis device results showed abandunce of food protein of animal origin, which was identified with the food samples taken from the real environment of these fish. Also two types of parasitic crustaceans were identified which are considered the most common external parasites infected fish ; these were Leranea worms, type Learnea cyprinacea, and fish lice Argulus, type Argulus foliaceus, this is with respect to Almmermrij fish taken from Al Muthan

This study was conducted to find out the effect of coriander seeds as feed additive on some hematological traits and wool growth and it's physical traits of Awassi ewes, blood samples were taken biweekly for blood picture, and wool samples were taken every 2.5 months for wool growth and its physical traits. Eighteen Awassi ewes at the age of 2-3 years were randomly divided equally into three groups (6each ) "body weight was considered".
Ewes in the first group kept as control and daily fed on concentrate diet (350 g/ head), while the second group was fed on the same diet contain (2.5%) of coriander seeds, while the third group was fed on same diet contain (5%) of coriander seeds. Also all animals were allowed to graze. Blood samples w

This study was included a collection of 90 milk samples from 30 cows ,30 ewe , and 30 does ,in mid-term of lactation period , 15 samples collected during summer and 15 samples during
winter for each other . All samples were subjected to microscopical examination after staining with Newman Limbert stain , to check : The mean number of SCC in cows was ( 426.600 ) cells / ml through the summer , while the mean number of SCC in the winter ( 893.300) cells / ml . The number of SCC in sheep samples were estimated (287.300) cells / ml in summer, and (980.000 ) cells / ml in winter . while the goat were given mean( 533.300) cell / ml in summer and ( ( 933.300) cell / ml in winter . This study gives the difference in milk quality between seaso

The study was conducted on 35 Cross breed cows. Three–Six years old suffering from dystocia in various regions of Baghdad province during 1996-2004. The incidence of dystocia was 29.16% (35 /120) from total calving cows. The result showed that the dystocia due to fetal and the dam were 85.71%(30/35) and 14.28%(5/35) respectively.The anterior presentation percentage from fetal dystocia cases was 93.3%(28/30) while the percentage of posterior presentation was 6.6% (2/30) from these cases. Cows delivered male and female calves were formed 77.14% (27/35) and 28.57% (10/35). The calves mortality percentage in both sex was 62.85% (22 / 35). The conclusion suggested that high incidence of dystocia due to fetus was in cows calving male calves

In the present work, the antinociceptive action was assayed in several experiment models in mice, Hot plate, writhing and formalin test. The alcoholic extract of MP leaves at a dose of 150mg/kg B.W and 300 mg/kgB.W showed antinociceptive effects in different methods, where the dose of 300 mg/kg B.W showed significant reduction of the nociception by acetic acid. In the formalin test, the extract (300mg/kg B.W) also significantly reduced painful stimulus in both phases of the test. Treatment with extract (300mg/kg B.W) when given orally produced significant increase of the reaction time in hot plate test. These results showed that the leaves extract of MP contain active analgesic principles acting both centrally and peripherally.