This study was carried out to investigate the cytogenetic effect of Levofloxacin after In-ovo inoculation. Forty eight fertilized eggs were used, divided equally into eight groups and inoculated through shell puncture above air cell with materials in equestion and incubated for two different periods three and seven days. They were assigned as group one and group two 10 µg Levofloxacin, group three and group four 20µg levofloxacin, group five and group six considered positive control 0.05 µg Mitomycin-C, group seven and group eight (Phosphate Buffer Saline) considered negative control groups. All eggs from in-ovo experiments were subjected to cytogenetic tests, such as Mitotic Inde
... Show MoreThis study was designed to evaluate the levels of interleukins (2, 6 and 8) in immunized white rats by killed whole cell antigens of Salmonella enterica subspecies typhimurium and sonicated Cryptococcus neoformans, and using ELISA in day 10, 20, 30, 40 and 50 after immunization one hundred white rats of both sexes divided into five groups (20 rats for each). The first group was immunized by killed whole cell antigens of Salmonella enterica subspecies typhimurium (9× 108 CFU /ml) and sonicated Cryptococcus neoformans (1000 µg/ml), The second was immunized by killed whole cell antigens of Salmonella enterica subspecies typhimurium
... Show MoreThe aims of this study were to detect the changes in microflora and incidence of vaginitis in ewes after using of intravaginal sponges. Twenty five Awasi ewes were divided randomly into two groups (1st group included 9 animals and 2nd group included 16 animals). Both groups were treated with intravaginal sponges (20 mg) for 12 and 10 days respectively. Swabs were taken from vagina before insertion of sponges and immediately after sponges’ removal. The results showed that, only 2 ewes in 1st group exhibited estrus while all ewes in 2nd group exhibited estrus. Escherichia coli was the most predominant bacteria before insertion and withdrawal of sponges (66.66 a
... Show MoreArabian horses are regarded as the most famous breed for speed for long distances due to their genetic superiority. The aims of the study were to focus on the effect of different running speeds on the muscular tissues (Myoglobin, creatine kinase, aspartate transaminase), lactate dehydrogenase and synovial fluid (creatine kinase, aspartate transaminase and lactate dehydrogenase). Non-trained eight local Iraqi Arabian horses were run on soil track for a distance (1) Km, walking, trot, canter, and gallop. Blood and synovial fluid samples were collected before and after running. The biochemical parameters of serum showed gradual increase according to each type of speed. All of them were significant at P≤0.01. The biochemical p
... Show MoreThe study is performed to investigate the cardiotoxic effect of two season oleander leave extract in rabbits. The study involved a collection of the Nerium oleander leaves of two seasons (summer and winter) taken from Baghdad. The leaves were prepared by drying and grinding, and the extraction was carried by soxhlet apparatus with organic solvent (hexane). The toxicity was carried out on rabbits by giving 10% of the median lethal dose (LD50) for four weeks by considering different electrocardiography parameters and cardiac histopathological study after two weeks and four weeks of daily treatment. The electrocardiography parameters include R-R duration, T amplitude and Q-T duration values. The results of
... Show MoreFour hundred fecal samples from diarrheic calves of both sexes (1-60 days old) from four Iraqi Mid-Euphrates governorates (Babylon, Al-Najaf, Karbala and Al-Qadissiyiah) were collected to investigate rotavirus type A infection by using Real-Time Polymerase Chain Reaction technique. The results showed that infection rates were (14%), while the high rate (20%) was found in age of (1-30 days), however non-significant values were related to animal gender. Moreover, a high infection was recorded at January (29.5%) and the rotraviral infection was recorded in four provinces but the higher was in Babylon (23%). The clinical examination of infected calves showed voluminous watery diarrhea, profound weakness and mild depr
... Show MoreThis study aimed to evaluate-the inhibitory effects of ethanolic and-chloroformic-extracts of local medicinal plants as Oak (Quercus-acuta), Thyme (Thymus vulgaris) and Cinnamon (Cinnamomum-zeylanicum) prepared in different concentrations (50, 100, 200 and 400 mg/ml) against the growth of Listeria-monocytogenes isolated from infected humans and animals and comparing their activity with effectiveness of the standard antibiotics. The results showed detection of Listeria-monocytogenes in 3 of (50) blood samples collected from aborted woman making 6% samples, while the detection rates of Listeria-monocytogenes in milk samples collected from sheep and cattle were 4
... Show MoreThe present study was conducted during the period from September 2015 until February 2016. 100 fecal samples were collected from 60 sheep and 40 goats for diagnosis of Cryptosporidium parasite from diverse areas in Al-Qadisiyah province. The study amid to know the genetic characters of Cryptosporidium spp. parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species. This study was done on the sheep and goat at first time in the middle region of Iraq and the identified species were recorded in NCBI-Genbank database. In sheep, the results of positive infected samples was (40%) while, in the g
... Show MoreThe aim of this study was to investigate the effect of anti-riboflavin factor in dried tomato seed meal on some physiological and biochemical traits of broiler. This study was conducted at the poultry farm of the Veterinary Medicine College at Baghdad University. A total of (150 broiler chicks) were used in this study for 56 days. The chicks were randomly distributed into five treated groups, 30 chicks for each period. Chicks at the age of 3 days were fed Ad libitum during the whole period and dried tomato seed meal was added to the diets of birds at the concentration of 0, 2.5, 5, 7.5 and 10% respectively. The results revealed that dietary supplementation with different levels of dried tomato seed meal
... Show MoreThe aim of this study was to evaluate three regimens in induction and maintenance of general anesthesia in local breed bucks and the investigation of the best protocol of them based on different parameters. Eighteen healthy adult bucks weighing (27.50 ± 0.682 kg), aged (1.989 ± 0.135 years) were used. Animals were divided randomly into three equal groups to undergo pre-experiment preparation. All groups were premedicated intravenously with (0.5 mg/ kg) Diazepam, ten min. Later, the induction for first group was done by Propofol (3 mg/kg B.W), second group was done by Ketamine Hcl (2 mg/kg B.W), while in the third group was done by Thiopental sodium (3mg/kg B.W). After intubation with suitable endotrac
... Show MoreThe current study was designed to determine the infection rate of intestinal protozoa in sheep and their handlers, and in their drinking water, in Wasit province, so as to study the relationship between contamination of drinking water with protozoal pathogen and infection in sheep and handlers in the period from the beginning of September 2015 to the end of February 2016. Two hundred eighty fecal samples were collected: (180) samples from sheep and 100 samples from human (50 handlers and 50 from non handlers), 50 drinking water samples were collected (18 samples; human tap water and RO bottled water and 32 samples river water). All samples were examined by conventional methods (direct moist smears, flotation with
... Show MoreThe study was carried out to evaluate histological changes induced by Aluminum chloride in Wistar rats. Forty rats were uniformly divided into two different groups: The control group were given distilled water during period of experiment and the first group were given oral daily dose of Aluminium chloride (37 mg\kg B.W). After 60 days of treatment rats were sacrificed. Liver, kidney and spleen were processed and stained with Haematoxylin and Eosin the lesions of these organs were characterized by degeneration, necrosis, and congestion which meant that Aluminum chloride was nephrotoxic as well as hepatotoxic in nature. Microscopic examination results showed that Aluminum chloride exposure was harmful to the histol
... Show MoreA total of 200 samples were collected from 100 goats from more than one regions within the three governorates (Sulaimani, Erbil and Duhok); all these samples were tested by California mastitis test and these showed the following results: 103 samples (51.50%) were negative in all governorates, 28(14.00%) were trace, 35(17.50%) were (+) mark and 34(17.00%) were (++) mark. Clinical mastitis was observed on 93 out of 200 half udder of goats, in the three governorates; 37(18.5%) were acute mastitis, 27(13.5%) were sub acute mastitis and 19(9.5%) were Chronic mastitis. Examination of extracted DNA by Real Time Polymerase Chain Reaction out of 200 DNA extracted from milk samples of goats only 10 (5.00%) were positive fo
... Show MoreThe purpose of the present study is to determine and compare the anticoagulants, Ethylene diamine tetra acetic acid, Sodium oxalate, Sodium citrate and heparin in blood samples of horse and bull, through estimation of (Hemoglobin, Packed Cell Volume and Plasma Platelets Count) and plasma physical properties (clarity and volume) and some chemical properties (pH and calcium ion concentration). Five Blood samples were obtained from five stallions and five bulls (20 ml/sample) and were divided as 5 ml in four test tubes containing Ethylene diamine tetra acetic acid, sodium oxalate, sodium citrate and heparin at the College of Veterinary Medicine, University of Diyala. Hemoglobin and packed cell volume were esti
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