The present study was conducted during the period from September 2015 until February 2016. 100 fecal samples were collected from 60 sheep and 40 goats for diagnosis of Cryptosporidium parasite from diverse areas in Al-Qadisiyah province. The study amid to know the genetic characters of Cryptosporidium spp. parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species. This study was done on the sheep and goat at first time in the middle region of Iraq and the identified species were recorded in NCBI-Genbank database. In sheep, the results of positive infected samples was (40%) while, in the g
... Show MoreBovine rotavirus was successfully isolated in vero cell line and secondary calf kidney cell culture from faecal samples of newly bom calves affected with diarrhoea. The isolated viruses were cultivated from cell culture produced cytopathogenic effect which consisted of
... Show MoreThis study was designed to investigate the correlation of various stress factors (PH, moisture contents, temp., and soil texture) on the ability of E.coli OI57:H7 to persist on/in soil on a dairy farm reared under field conditions at the college of Agriculture /University of Bagdad. The prevalence of E. coli O157:H7 in soil samples was determined for the period January to June 2012. The surface kinetics of E.coli O157:H7 onto the soil (surface kinetics), were theoretically achieved by dividing the farm into 3 zones starting from the fens (Z1),5m, and 10 m (Z5, and Z10, respectively) from the farm in three direction (right, left and rear of the farm) . While the depth kinetics were achie
... Show MoreOut of 107 COW milk samples, 44(41.12%) yielded bacterial growth. The isolates were identified according to their cultural, morphological and biochemical characteristics as Staphylococcus aureus
This study was carried out to investigate 87 lungs of camel slaughtered in Al-Najaf
... Show Moreتهدف الدراسة الحالية التحري عن تأثير المستخلص المائي لثمار نباا الطبا Tribulus terrestis فاي بعا
المعايير الكيموحياتية وهي الفوسفاتيز الطاعادي والحدياد والفوسافا والداوديوم و البوتاسايوم واسلدوساتيرول و الكورتيكاو
ستيرود في ثلاثين أرنب ا مان الاوكور البالغاة قسام حيواناا التررباة علا ساتة مرااميا المرموعاة اسولا هاي مرموعاة
السيبرة ، و المرموعة الثانية جرع ب 051 ملغم\كغم\يوم من المستخلص المائي للطبا ، والمر
هدفت الدراسة الحالية لتحديد تاثير نبات الحنظل فى بعض الصفات الفسلجية كوزن الجسم ، بعض معايير الدم
ووظيفية الغدة الدرقية لذكور الارانب انجزت تجربتين لستين ذكر ارنب بالغ و قسمت بشكل متساوي وعشوائي الى 03
ارنب في كل تجربة . تحوي كل منها على ثلاث مجاميع معالجة وثلاث مجاميع سيطرة وكل مجموعة ضمت 5 ارانب .
في التجربة الاولى والثانية وبشكل منفصل , اعدت الارانب المعالجة) (T1, T2 and T3 groups وجرعت في التجربة
الاولى بجرعة
Stool samples from 182 asymptomatic patients were collected from two hospitals in Baghdad (Al-Yarmok and Central Child) during the period from the beginning of March to the end of June/2014 and subjected to microscopic and ELISA diagnostic techniques for Giardia lamblia detection; the sensitivity and specificity for ELISA were calculated. Cysts of G. lamblia were observed in 37 and 49 stool samples of asymptomatic patients from a total 182 examined samples with prevalence recorded 20.32, 26.92% by direct examination and ELISA respectively. Of 145 samples showed negative result in microscope examination, 12 samples were positive in ELISA, sensitivity and specificity of ELISA were
... Show MoreInfection with Toxoplasma gondii is a cause of fetal death since T. gondii can be transmitted to the fetus through the placenta (transplacental) from an infected mother or at vaginal delivery. Blood obtained from women and sheep to confirm their infection with toxoplasmosis by using Enzyme Linked Immunosorbant Assay test (ELISA) to ditective positive specific anti-Toxoplasma (IgM, IgG and IgM, or IgG) antibodies. This study used two methodes to extract DNA (the first one was a standard extraction commercial method (CM-PCR) of genomic DNA using a commercial kit (Promega, USA), and the second one was the direct heat DNA extraction using microwave oven (MW-PCR) for whole blood sam
... Show MoreThis work was done to investigate Nocardiosis in cattle in order to evaluate the incidence
... Show More|
Our objective in this work was to estimate normal values of biochemical parameters in laboratory animals in Iraq. Values were pooled from studies conducted in Iraqi universities using a meta-analysis approach. Pertinent peer-reviewed published studies were retrieved from the Iraqi Academic Scientific Journals (IASJ) database. Random-effects inverse-variance model was used to obtain pooled means of the parameters from the set of studies qualified for the analysis. Among a total of 460 records identified from IASJ, 264 records were eligible for the statistical analysis, which reported 102 values for different biochemical parameters. The mean of the sample size for normal animals that was used in the studies includ |
The present study performed to evaluate the impact of addition of
Thymbra spicata leaves powder of aflatoxin contaminated ration in
reduction of the aflatoxin immuno suppressive effect in the immune
response induced by Newcastle disease vaccine in broilers.
The study conducted in three groups, The first group fed aflatoxin free
ration, second group fed aflatoxin contaminated ration without any
treatment, while the third group fed aflatoxin contaminated ration
supplemented of 2% Thymbra spicata leaves powder.
All chick groups were vaccinated twice with Newcastle disease vaccine
(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate the
humeral immune response at 6, 19 and 31 days of age. Infec
The objective of the study was to investigate the effect of Ericsson Albumin Method to separate Y- from X-bearing sperms in mice. The sperms were obtained from caudal epididymis of male mice and prepared by Ericsson method using albumin in two concentrations (8% and 18%) fallowed by intra-peritoneal insemination of female mice. The study showed there was a significant increase (P>0.05) in pregnancy rate in female mice that inseminated by sperms were prepared by Ericsson method. Also, the results demonstrated that Ericsson method was found (75%) effective for male gender selection compared to conventional method (51.25%) without separation of Y- from X-bearing sperms.
The study was designed to determine the infection rate of Toxoplasma gondii in the rabbits and histopathological changes of infected internal organs in mice. A total of 60 blood samples were collected from clinically healthy rabbits from different region of Baghdad city for the serological detection of T. gondii infection. Biological assay in mice was performed by intraperitoneal inoculation of 0.1 ml digested organs suspension, (lungs, heart, liver, spleen, kidney, muscles, and brain) during the period from October 2013 until July 2014. The results revealed histopathological changes in bioassay mice infected by T. gondii isolated from domestic rabbit, no histopathological changes in th
... Show Moreيهدف البحث إلى إيجاد التأثير الفيزيائي الحياتي للأشعة الكهرومغناطيسية على بعض معاملات الدم مثل حجم الكريات المرصوصة والتعداد الكلي والتفريقي لخلايا الدم البيض وعلاقتها بتغير كثافة القدرة بوحدات مايكروواط/سم2. حيث استُعمل أربع مجاميع من إناث الفئران البيض (5/ مجموعة). المجموعة الأولى عدت مجموعة السيطرة، المجموعة الثانية عرضت لـ 100مايكروواط /سم2، المجموعة الثالثة عرضت لـ 150 ما
... Show MoreThis research was designed to study the effect of tow levels of Zingiber officinale on Body weight , Hb , PCV ,WBCs account , Lymphocytes , Neutrophiles accounts GOT and ALP liver enzymes in Awassi ewe’s blood , Nine Awassi ewes aged 1.5 years were divided into 3 equal groups as followed : Group A was treated by 10 gm Zingiber officinale / animal /daily (2.5%) of diet While group B were treated with 20 gm Zingiber officinale / animal daily (5%) of diet and group C considered as Control group without given Zingiber officinale with diet ,the results showed that signific
... Show Moreتهدف الدراسة الحالية لوصف بعض التراكيب النسجية والخصائص الكيميائية النسجية للخلايا العمودية (الامتصاصيه) والخلايا الكاسية في أمعاء سمك الكارب . أجريت الدراسة على خمسة عشرعينة من سمك الكارب البالغ تم اصطيادها من نهر الفرات , معدل وزنها (2100 ± 81) غرام وطولها القياسي (50.4 ± 3.1) سم بعد القتل مباشرة . تم إجراء شق خلال الخط ألبطني الوسطي لكل سمكه تماما أماميا لفتحة المخرج لإخراج الأمعاء, أخرجت عينات ال
... Show MoreThe use of herbal plants as food additives in animal nutrition to enhance meat processing efficiency and meat quality has been reviewed. Today, the consumer demand is safety, nutritive value, taste, uniformity, meat variety, and good appearance of meat products. Thus, to meet the consumers' demand, development of product and research should be improved. Studies have been shown that the use of herbs, spices, and their extracts are of the major interventions, which were adopted in the industry of the meat for improving its quality traits. In the present paper, the most recent literature about use of bioactive compounds in herbal plants for evaluating a number of parameters related to meat quality, including fat
... Show MoreThe current study was performed an investigation in seropositive stray cats with Toxoplasma gondii and to correlate the results of pathological lesions with seropositive results of the cats. To achieve these goals, fifty blood samples and specimens from internal organs (liver, spleen, brain, kidney, intestine and lung) of stray cats were collected from different area of Baghdad Province during the period 1.10.2011-1.10.2012. Seropositive ELISA-IgG was demonstrated that 66 % of stray cats while female expressed (75 %) of seropositive ELISA-IgG were higher than male (30% ) and animals with average age 2months showed high percentage of seropositive ELISA-IgG (100%) as compared with age (adult cat) that expressed( 63.82 %) of seropositive. T
... Show MoreA total of 100 animals were examined during the period of beginning of September - 2010 till the end of March 2011 at dept of Microbiology college of Veterinary Medicine Baghdad University Baghdad Iraq. These animals include 50 cow and 50 sheep. Hairs and scales were submitted to direct KOH mount smear and culture on modified Sabouraud's Dextrose agar medium The direct smear was positive in 40 ( 80%) for both cows and sheep while the growth of dermatophyte was positive in 35 ( 70%) and 38 ( 76%) for cows and sheep respectively. Species identification revealed the presence of Trichophyton rubrum ( 19 isolates ) Trichophyton verrucosum ( 10 isolates ) Trichophyton mentagrophytes ( 5 isolates ) and Microsporum canis ( one isolate ) in cow w
... Show MoreThis study was conducted in the special nursery in Mansour - Baghdad at 2011. to study the effect nitrogen 05 and 02 grams per liter in the form of urea as well as spraying four plant extracts which are Glycyrrhiz glabra uritica dioica Allium sativum Trigonella foenum – graecum and concentration of 5 grams per liter of distilled water and every extract plant in addition to the control treatment. Treatments were applied three times and the first was applied at 15-5-2011and the following applications were 15 days after the first application. Experiment were conducts using complete randomized block design with three replicates. Results, were analyzed using least significant differences at 5% probability. The results are summarized as foll
... Show MoreThe aim of this study was to evaluate the cellular immune responses of salt-Extractable Brucella abortus S19 antigens with immunoadjuvant soluble βeta-glucan in BALB/C mice later challenged with B. abortus virulent strain. The 0.72mg/ml of SEBA was used according to the results obtained from experiment to determine the macrophages Nitric oxide production and delayed type hypersensitivity test. One hundred BALB/C mice were divided into four groups. G1 were injected i.p with 0.2 ml of saline, G2 were vaccinated S.C with 0.1ml (108 CFU/mouse) of B. abortus S19, G3 were vaccinated i.p. with 0.2 ml of salt-Extractable Brucella abortus S19 antigens and G4 were vaccinated
... Show MoreThis study was conducted to investigate the protective role of polyphenolic compounds extracted from olive (Oleaeuropae) to contrast the damaging effects of 1% hydrogen peroxide on liver functions in male rats. Crude polyphenolic compounds were extracted from fruits of black olive by 95% methanolic extraction method. Twenty adult male rats (200-220gm.) were randomly divided into four equals groups and treated daily for 30 days. Rats in the first group received tap water (orally)and considered as control group, animals of second group received 1% H2O2 in drinking water . The rats in the third group received 1% H2O2 in drinking water plus 200mg/kgB.W. of crude polyphenolic compounds while animals in the fourth group received 200mg/kg B.W.
... Show MoreA sample of (700) seven hundred out of (3000) three thousend randamly collected
The structure of the indigenous chickens spleen during the post-hatching period was determined by gross and light microscopical examination by using Hematoxylin and eosin and Massons Trichrome staining techniques. At one day old chicks the spleen was rounded in shape, pink in color. At two weeks old chicks the spleen was triangular in shape. At the progress of the aged the color of spleen became red-brown. In all ages the spleen consisted of white pulp and red pulp which were fused together. The spleen was encapsulated by thin connective tissue capsule contain few smooth muscles, the trabiculi were rare and thin. The red pulp consisted of venous sinuses surrounded by lymphatic cords. The white pulp consisted of p
... Show MoreTo investigate the impact of Enteococcus faecium as probiotic in layer chicks,
this bacteria was given in feed at a dose rate of 1.5 gm per kg feed, each kg of
feed contained 5×1011 cfu along the period of the study (57 days). 150 one day
old males were divided into five groups, 30 males each. The 1st group was given
the probiotic only in the feed, the 2nd group was given the probiotic and exposed
to high temperature and bad ventilation as a stress, and the third group was
given the probiotic with chlortetracycline in feed while the fourth group was
given the probiotic and then infected with E. coli on 21 day of age and the fifth
group was given feed with out probiotic as the control group. The results
showed
This experiment aimed to study the effect of diet supplementation with
feed enzyme (Poultrygrow) or chromium yeast (Biochrom) on the intestinal
microbial balance of broiler chicks. A total of 300 broiler chicks, one day old,
were randomly allocated into three treatment groups and named T1, T2 and T3
respectively. Chicks in T1 used as control while chicks in T2 and T3 were feed
diets with two feed additives, Poultrygrow and Biochrom, respectively.
The data show that the two feed additives were decreased the total
bacterial count and coliform count in crop, Jujinum and large intestine but the
statistical analysis showed that the effect were not significant. Supplementation
of diet with Biochrom were significantly
The antimicrobial activity of Lactobacilli has been widely exploited for prevention of
food –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially in
children, because of bacteriocin activity and the importance of herbal drugs, hence this study
was designed to evaluate the synergistic effect of plant extract and bacteriocin produced by
Lactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.
1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.
2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min and
then purified by two steps method including extraction with n-butanol followed by gel
filtration chromat
من اوراق نبات الطرطيع glycosides والكلايكوسيدات alkaloidsتم استخلاص القلويدات schanginia حيث استخدمت التراكيز التالية Lashmania major ودراسة تأثيرها على نمو بروماستكوت اللشمانيا الجلدية aegyptiaca ملغم/مل ) لكل من القلويدات والكلايكوسيدات فأظهرت النتائج ان القلويدات المستخلصة من نبات 25 , 10 , 5 , 1 , 0.1 ( للشمانيا الجلدية promastigote ملغم/مل) ذات تأثير فعال أقوى على نمو الشكل المسوط الأولي 5الطرطيع بتركيز ( %( صفرا بعد أربعة أيام من التنمية وبين
... Show MoreIn this study a pyloroplasty technique was designed by using a single layer simple interrupted extra-mucosal suture pattern on pyloric region in dogs. Nine adult dogs were used to induce longitudinal surgical incision in the pyloric region under general anesthesia using a combination of 15mg/kg B.W. ketamine hydrochloride 5% and 5 mg/kg B.W Xylazine hydrochloride 2%. The incision was closed by pyloroplasty technique (longitudinal incision for whole layers with transverse suturing) using single layer simple interrupted extra mucosal technique. The examination achieved by daily clinical observation, radiological finding, at the end of 21st day post operation, macroscopical and histopathological examination at
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