Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 g
... Show MoreThe aim of this study was to evaluate virulence of local isolated avian infectious laryngotracheitis virus in experimentally infected chicken. Forty chickens 10 weeks old were used for the experimental infection with the locally isolated infectious laryngotracheitis virus. Chickens were divided into three groups, the first group consisted from 20 chickens infected with isolated infectious laryngotracheitis virus (2×104.16 TCID 50/50 µl) via eyes and mouth drops (one drop for each). The second group consisted of 10 chickens (non-infected) in contact with infected group inoculated with maintenance media (Minimum essential medium) on their eyes, to observe if the infected group can spread the virus. The
... Show MoreThis study was conducted to compare some of anatomical features and histological structure of the liver in three species of birds that varied in their size, taxonomy, and food environment. The study revealed that the liver in the mallard, gull and white-eared bulbul was bilobed big gland, and the left lobe was smaller than the right one. It was observed that the two lobes in mallard were undivided, while the right lobe in gull has two parts, whereas the left lobe in white-eared bulbul is subdivided into two parts. Histologically, the liver capsule in gull appeared thicker than other two species. The hepatic parenchyma was not shown to be clearly defined lobules in three species. The hepatocytes were organized rad
... Show MoreAn investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive
... Show MoreThe study was carried out to evaluate histological changes induced by Aluminum chloride in Wistar rats. Forty rats were uniformly divided into two different groups: The control group were given distilled water during period of experiment and the first group were given oral daily dose of Aluminium chloride (37 mg\kg B.W). After 60 days of treatment rats were sacrificed. Liver, kidney and spleen were processed and stained with Haematoxylin and Eosin the lesions of these organs were characterized by degeneration, necrosis, and congestion which meant that Aluminum chloride was nephrotoxic as well as hepatotoxic in nature. Microscopic examination results showed that Aluminum chloride exposure was harmful to the histol
... Show MoreIn this study a pyloroplasty technique was designed by using a single layer simple interrupted extra-mucosal suture pattern on pyloric region in dogs. Nine adult dogs were used to induce longitudinal surgical incision in the pyloric region under general anesthesia using a combination of 15mg/kg B.W. ketamine hydrochloride 5% and 5 mg/kg B.W Xylazine hydrochloride 2%. The incision was closed by pyloroplasty technique (longitudinal incision for whole layers with transverse suturing) using single layer simple interrupted extra mucosal technique. The examination achieved by daily clinical observation, radiological finding, at the end of 21st day post operation, macroscopical and histopathological examination at
... Show Moreمن اوراق نبات الطرطيع glycosides والكلايكوسيدات alkaloidsتم استخلاص القلويدات schanginia حيث استخدمت التراكيز التالية Lashmania major ودراسة تأثيرها على نمو بروماستكوت اللشمانيا الجلدية aegyptiaca ملغم/مل ) لكل من القلويدات والكلايكوسيدات فأظهرت النتائج ان القلويدات المستخلصة من نبات 25 , 10 , 5 , 1 , 0.1 ( للشمانيا الجلدية promastigote ملغم/مل) ذات تأثير فعال أقوى على نمو الشكل المسوط الأولي 5الطرطيع بتركيز ( %( صفرا بعد أربعة أيام من التنمية وبين
... Show MoreThe relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb con
... Show MoreThis study was designed to isolate of Enterohaemoragic E.coli O157:H7 from feces and urine in cattle. 175 samples (80 fecal and 95 urine) were collected to isolate EHEC O157:H7 during the period from March to June 2014 from Al- Hindia slaughter house from local breeding cattle, aged between 1-2 years and over 2 years, and the samples were subjected to culture on specific media Cefixime Tellurit- Sorbitol MacConkey agar at 37 ˚C for 18-24 hrs. then the isolates were identified by biochemical tests (Indol test, Potassium cyanid test) and seriologically by Latex Kit test. The results showed that 73(91.25%) out of 80 fecal samples and 39(41%) out of 95 urine samples were E.coli O157:H7 positive, th
... Show MoreThis study is planned to evaluate the efficacy of two biological matrices represented by autologous platelet rich fibrin matrix, as well as a cross linked decellularized caprine pericardial extracellular matrix on enhancing healing of the experimentally severed superficial digital flexor tendon in a goat model. It was carried out on 48 adult apparently healthy bucks, which were divided randomly into three equal groups. Under the effect of sedative and local ring block anesthesia, superficial digital flexor tendon was severed at the mid metacarpal region of the right forelimb. In the first control group, tenorrhaphy was performed and left without additives. While in the second group the tenorrhaphy site was wrappe
... Show Moreأجريت هذه الدراسة لمعرفة تأثير إضافة مستويات مختلفة من مسحوق خليط الثوم وعرق السوس المضافةإلى العليقة في الصفات الإنتاجية لفروج اللحم. وإستعمل في التجربة 180 فرخاً من سلالة (Ross-308) بعمريوم واحد، وزعت عشوائيا على أربعة معاملات تجريبية بواقع (3) مكررات لكل معاملة (15 فرخ / مكرر). وكانت المعاملات: الأولى المقارنة خالية من الأضافات، الثانية والثالثة والرابعة تضمنت ( 0.25 و0.50 و 1) % من مسحوق خليط الثوم وعرق ال
... Show MoreThis study is designed to determin the protective role of pomegranate seed oil against deleterious effect induced by sodium fluoride on serum proteins in rats. Forty (40) adult female rats were randomly divided into four equal group (10/group) and treated daily for 40 days as following: Group C administered distilled water (control), group T1 received sodium fluoride 120 ppm/liter in drinking water, group T2 received both sodium fluoride 120 ppm/liter in drinking water and Pometone 30 mg/kg B.w. orally and group T3 administered Pometone 30 mg/kg B.w. orally. Fasting blood samples were collected at 0, 20 and 40 days of the experimental periods and serum samples were aliquoted for estimation serum total protein, albumin and globulin concen
... Show MoreThis study is designed to investigate the effect of temperature on thyroid gland of 24 adult healthy female carp fish (12/ season). Neutral buffered formalin solution10% was used for fixation and routine histological techniques. Hemotoxline and Eosine and PAS stains were, also used. The results revealed that the thyroid tissue in carp fish was closely associated with the renal and sub pharyngeal tissue. Spleen and liver did not show any thyroid follicles. The thyroid tissue of carp fish consisted of disseminate thyroid follicles not surrounded by apparent capsule. Different sizes and shapes of thyroid follicles were observed. The follicular cells form tight epithelium. Para follicular cells were distributed among
... Show MoreMelatonin is the main molecule that transmits the signal of seasonal change to the neuroendocrine system in seasonal breeding species, through specific melatonin receptors, Melatonin Receptor Type 1A. In the present study, a total of 40 local Iraqi non pregnant goats were used to study the relationship betweenPolymorphism of Melatonin Receptor Type 1A (MTNR1A) gene with some reproductive performance. Animals were reared in a farm located inAl-Yusufiya/Baghdad. Animals were divided based on breading season into two groups, with 20 animals each as follows: goats which gave birth in expected breading season (January, Febreuary,and March), and goats which gave birth in unexpected breading season (October, November, and December). All goats w
... Show MoreThe study was conducted to investigate the effect of different concentrations of sodium hypochlorite (sodium dichloro iso cyanurate) in the drinking water on the percentages of serum proteins of broilers chicks at 4 and 7 weeks of age. Two hundred forty Hubbard chicks one day – old aged were divided randomly into four equal groups, each group was divided into two replicate. The four groups were as follows: Treatment 1: Adding 6.68 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 2: Adding 13.36 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 3: Adding 20.04 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 4: Supplying water without any additive (con
... Show MoreThe histological study showed that the retina of falcon"s eyeball was thin at the periphery
and ranges between 0.6-0.8 μ but it was thick at center 1.68-2.64 μ. The retina consists from
ten layers: pigmented epithelium, rods and cons layer, external nuclear layer, external
plexiform layer, internal nuclear layer, internal plexiform layer, Gangilionic cells layer,
neurofibres layer and internal limiting membrane layer. The ultra-structural study stated that
the rods and cons layers contained single rods with single and double cons. The retina
characterized by lacking of the oil droplets in the internal segments of rods with narrow
external plexiform layer in order to form network connecting rods and cones, horizonta
A total of 80 specimens of common carp Cyprinus carpio were used in present study ranges between 142-156 g with 150 g in average body weight, and between 22-27cm with 25cm in average body length to investigate the effect of magnetized water in blood pictures of fish as well as Aeromonas hydrophila pathogenic bacteria. Fish were randomly distributed using aquarium with dimension 70X40X40 cm upon four treatments, with two replicates for each treatment using magnetized water with different intensity ( 500 , 1000 , 1500 gauss) , while the control treatment free of magnetized water through the period of four weeks . Results shows that the bacteria count were decreased in such magnetized water in comparison with control treatment . The results
... Show MoreThe aim of this study is to detect the prevalence of macroscopic sarcocystosis of 480 wild birds ( 82 Pygmy cormorant, 34 Black Stork, 99Common Gull,74 little Egret, 91Raven and 100 Mallard) were hunted from October 2009 to September 2010 in Al Rashdia areae /Baghdad . The macroscopic infection total was 9.58%. it was regarded to monthly variation. It was 27.5% in February and 2.5% in August. The highest infection rate was in the thorax muscles (6.2%) and the lowest in the neck muscles (1.6%), Also the macroscopic infection rate was higher (13.5%) in males than females (5%).
An infectious syndrome of broiler chickens characterized by. stunted growth, poor feathering, leg weakness, poor feed conversion, and loss of pigmentation of the skin. The syndrome was initially reported in Netherland (Kouwenhoven et al., 197848), and have since been described in England (Bracewell and Wyeth, 1981), U.S.A. (page et al., 1982), Australia (pass et al.,1982) and Canada (Riddell and Derow, 1982). The various names of the syndrome reflects the variety of clinical signs and gross lesions .observed, and although reported conditions share many clinical signs and pathological changes, it is not certain that all reports describe the same disease entity.
The epididymal region of the common quail was located on the dorso-medial as
... Show MoreThe effect of vit.E in feed blocks to ewes were studied in ewes on the
following parameters; Hemoglobin concentration, Packed Cell Volume,
differential leukocytic count, total protein, albumin, and globulin ratios. There
were a significant decrease in Hb concentration & PCV after two,& four weeks,
there were no significant differences in the percentage of lymphocytes,
neutrophils,& eiosinophils, & with a significant increase in monocyte
percentage.
There was no significant different in total protein ratio, while the ratio of
albumin decreased significantly after 2 & 4 weeks, with a significant increase in
the globulin ratio at the same period.
This study was conducted on 80 cases of damaged kidneys received by
poultry disease diagnostic centers in Baghdad. Four microorganisms were isolated
E.coli , Proteus milabilis , Pseudomonas aregenosa and Klebsiella pneumonia
and formed 55, 22.5 , 17.5 ,and 5% of the infection respectively . While no any
kind of bacteria were isolated from kidneys of healthy chicks. To be sure the E.
coli could be a cause of kidney damage. An experimental infection with E.coli
was carried out on 40 chicks, three weeks old, and randomly divided into two
groups. Chicks of the first group was inoculated intravenously with 0.5 ml of field
isolated E. coli suspension containing 2x107 colony forming unit (CFU), while the
second group
This study is considered the first trail study in our country to apply In ovo-vaccination to immunize chicks against avian viral diseases. This method was conducted by using 150 chick embryos classified into three equal groups (50 embryo each group). The first group was inoculated with Newcastle disease virus-NDV (B1-strain). Second group was vaccineted with NDV. and GV( Gambaro virus). All vaccinated groups were inoculated in the amniotic sac, the
third group (control group) was inoculated with sterile phosphate buffered saline. Result should the both vaccine did not reveal any negative effect on the percentage of hatchability and liaviability of the vaccinated chicks.
Hemagglutination inhibition (HI) , ELISA and challenge test we
This study was designed to isolate and identify the type of moulds and their
percentage of distribution with pathological study of highly moulds isolated from
(130)samples were included in this study with divided into (30)samples dried
milk,(30).samples baby food ,(30)samples raw milk , (40) samples dairy products .
The results revealed the percentage of infection according to total count of
isolation in raw milk were 100 % dairy product 65 %. The most common fungi
isolated from raw milk were Asp.niger of 16.2 % Asp. fumigatus of 15 % Asp.flavus
of 13.7 % Pencillium spp. 12.5 % . From dairy products were isolated with
percentage of Asp. Fumigatus 34.9% Geotrichum spp . of 23 % ,Asp. nager of 11.5
% . Pathologic
It was possible to obtain 17 isolates of Pasteurella multocida from
infected laying chickens with fowl cholera. Three isolates were from the chronic
phases of the naturally infected chickens and other fourteen were from the acute
phases. The identification of these isolates was considered. Fifteen isolates
belong to the sub species multocida and two isolates to gallicida, ten isolates
belong to the SE-190 isolate and one to SE-077, while six isolates were not
identified. Twelve of the isolates have a capsule. The LD50s of these isolates
were varied. The results of sensitivity to antibacterial drugs revieled that all
isolates were sensitive to Penicillin and resistance to Lincomycin with various
degrees of sensi
The pharmacokinetic of chloramphenicol was studied in 8 calves .These calves were infected experimentally with Samonella typhimorium The
... Show MoreFifty isolates of Vibrio cholerae obtained from different areas in Iraq from patients with acute secretory diarrhea were diagnosed serologically in central public health laboratory(CPHL), Two different methods were used for detection cholera toxin production ,the first based on the degradation of Nicotin Amide Adinine Dinucleotide( NAD) by Cholera toxin (CT). and the second was by detection CT gene by polymerase chain reaction (PCR).All Vibrio cholerae were cultured in alkaline peptone water, out of 50 isolates 46 (92%) were positive when we used NAD for the detection of cholera toxin(CT).Whereas all isolates gave positive results by PCR. From these data we conclude that the degradation of NAD by CT is simple and can be carried out in sm
... Show MoreDiabetes mellitus has been reported to be accompanied by thyroid and testicular dysfunctions. The objective of this study was to investigate the effect of Quercus infectoria galls (QIg) extract on the thyroid gland and testicular functions in diabetic rats. Sixteen rats were randomly divided into four equal groups, consisting of normal control, diabetic untreated control, diabetic treated with oral administration of 500 mg/kg BW and 1000 mg/kg BW, respectively for 15 days. Serum blood glucose, thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), testosterone (T), and luteinizing hormone (LH) were assessed. At the end of the experimental period, the rats were euthanized for histopathological analysis of thyroid gland
... Show MoreFourty five local buffaloes naturally infected with A. marginal age of 2-5 years. Clinical signs, present parasireamia haematological and biochemical changes were studied. Thin thick blood smears Stained with Gimsas stain were used to identify the parasite. The results indicated that most of the infected were suffering from mild form of infection and the 1I11p01”l11I1E' clinical signs observed were mild rise of body temperature, increase respiratory and plus rate, weal: ruminal contraction and generalized weakness. Palcness and iteration of mucous membrane, loss appetite, nasal and ocular discharge, coughing, salivation, atarcp, constipation, diarrhea, and rccumbancy. Statistical analysis showc increase (P<0.01) in body temperature
... Show More