This study was designed to investigate the effect of dill (Anethum graveolens) seeds in mitigating the complications of induced diabetes mellitus such as nephropathy of adult female rabbits. Thirty adult female rabbits were randomly assigned to 5 equal groups and treated daily for 45 days as follows: Control group (C) drenched tap water, control group intubated orally with 700 mg/kg B.W ethanolic extract of dill (T1), diabetic group drenched tap water (T2), diabetic group intubated orally700 mg/kg B.W of dill (T3) and diabetic group treated with insulin injection 3 I.U S/C (T4). Blood samples were collected by heart puncture at 0 and 45 days of experiment to assess renal efficiency: Serum creatinine, ser
... Show MoreThis study was carried out to identify the morphological, histological and histochemical features of the vagina of the adult guinea pig (Cavia porcellus). To perform such project, 14 adult guinea pigs at their diestrous period were bought from the local breeders directly. Animals were euthanized, dissected and subsequently specimens from the cranial, middle and caudal portions of the vagina were collected and fixed. Fixation was implemented by using 10% neutral buffered formalin and Bouin’s solution. Post routine processing such as dehydration, clearing, embedding and blocks preparation, tissue sections of 6 μm were prepared and stained by using hematoxylin-eosin, Masson’s Trichrome, Alcian blue and Peri
... Show MoreThe study was designed to determine the infection rate of Toxoplasma gondii in the rabbits and histopathological changes of infected internal organs in mice. A total of 60 blood samples were collected from clinically healthy rabbits from different region of Baghdad city for the serological detection of T. gondii infection. Biological assay in mice was performed by intraperitoneal inoculation of 0.1 ml digested organs suspension, (lungs, heart, liver, spleen, kidney, muscles, and brain) during the period from October 2013 until July 2014. The results revealed histopathological changes in bioassay mice infected by T. gondii isolated from domestic rabbit, no histopathological changes in th
... Show Moreيهدف البحث إلى إيجاد التأثير الفيزيائي الحياتي للأشعة الكهرومغناطيسية على بعض معاملات الدم مثل حجم الكريات المرصوصة والتعداد الكلي والتفريقي لخلايا الدم البيض وعلاقتها بتغير كثافة القدرة بوحدات مايكروواط/سم2. حيث استُعمل أربع مجاميع من إناث الفئران البيض (5/ مجموعة). المجموعة الأولى عدت مجموعة السيطرة، المجموعة الثانية عرضت لـ 100مايكروواط /سم2، المجموعة الثالثة عرضت لـ 150 ما
... Show MoreFour weeks old mice were used for experimental infection with isolated bovine parainfluenza type 3 virus. Mice were divided into three groups: The first group was infected by intracerebral route, the second group was infected by intranasal route and the third group was a control group. Experimental infection with isolated bovine parainfluenza type 3 virus in mice revealed that the isolated BPIV-3 was unable to kill 4 weeks old mice within 65 days when inoculated by intracerebral route, but isolated BPIV-3 was shown to kill (5 out of 10) 4 weeks old mice within 65 days when inoculated by intranasal route. There was no mortality in the control group within the same period. Two weeks old mice were used for experimen
... Show MoreThis study was carried out to investigate the effect of using alcoholic Ocimum basilicum and Olea europaea leaves extract, to study the antiviral activity against the Newcastle disease virus of poultry chicken embryo fibroblast monolayer culture. The extract was also decided for the chicken fibroblast culture and the concentrations (500, 250, 125, 100 and 50 µg /ml) of an alcoholic extract of Ocimum basilicum and Olea europaea leaves in maintenance media appeared to be nontoxic. Where the cytopathic effects of the Newcastle disease virus on chicken embryo fibroblast monolayer culture are well established so these concentrations were used to detect the antiviral activity
... Show MoreStool samples from 182 asymptomatic patients were collected from two hospitals in Baghdad (Al-Yarmok and Central Child) during the period from the beginning of March to the end of June/2014 and subjected to microscopic and ELISA diagnostic techniques for Giardia lamblia detection; the sensitivity and specificity for ELISA were calculated. Cysts of G. lamblia were observed in 37 and 49 stool samples of asymptomatic patients from a total 182 examined samples with prevalence recorded 20.32, 26.92% by direct examination and ELISA respectively. Of 145 samples showed negative result in microscope examination, 12 samples were positive in ELISA, sensitivity and specificity of ELISA were
... Show MoreInfection with Toxoplasma gondii is a cause of fetal death since T. gondii can be transmitted to the fetus through the placenta (transplacental) from an infected mother or at vaginal delivery. Blood obtained from women and sheep to confirm their infection with toxoplasmosis by using Enzyme Linked Immunosorbant Assay test (ELISA) to ditective positive specific anti-Toxoplasma (IgM, IgG and IgM, or IgG) antibodies. This study used two methodes to extract DNA (the first one was a standard extraction commercial method (CM-PCR) of genomic DNA using a commercial kit (Promega, USA), and the second one was the direct heat DNA extraction using microwave oven (MW-PCR) for whole blood sam
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Type 2 diabetes mellitus (T2DM) in dogs is a complex, multifactorial disease that is characterized by chronic hyperglycemia and insulin resistance. Current therapeutic options are often limited by side effects and variable efficacy, highlighting the need for more effective and safer treatments. This study assessed the therapeutic potential of ginger ethanolic extract (GEE), GEE-loaded chitosan nanoparticles (GEE-CNPs), and chitosan nanoparticles (CNPs) against T2DM in dogs. Twenty adult local breed mongrel dogs of both sexes, aged 7 to 13 months, with an average body weight of 10.4±0.76 kg, were included. The dogs were allocated into five groups (n=4 each): a non-diabetic, untreated Negative Control group, |
The aim of studyblood picture to know the effects of chronic toxicity of infected wheat added to ratios and fed for six months to one year to (20) rats of both sexes divided into two groups to study metabolic, blood chemistry and histopathological changes in organ and tissues .
All these studies performed to evaluate the toxic effects and health hazard in human and animal that used the infected wheat whith fungal spores in food , results on blood and chemistry parameters such RBCs counts, Hb, PCV, WBCs showed decreased in the treated group . The biochemichal changes noticed reduction in ALT, AP , BUN as well as reduction in total serum protein and changes in albumin. The intensity of changes in these parameters were positively related
Twenty Shami goats, aged 2-4 years at time of parturition were suffering from dystocia due to incomlete dilatation
... Show MoreSurvey on the distribution of Hydatiddosis in sheep in A Anbar province have
been done during period 1/1/2OO2to31/12/2002. This survey showed that
℅28.29 of sheep suffering from Hydatidosis also showed that the infection is
more in female than in male .The incidence was increased in cold season,
meanwhile the percentage of organ infection in both male & female being low in
liver but higher in lung and the infection the highest in organs, liver and lung
together.
This study lasted for eight months .The main objectives of the study were
to identify the main sources of raw milk pollution with Salmonella with a
special emphasis on determination of their serotypes. The relationship between
their resistance to antibiotics and their plasmide contents were also studied.
Six hundred raw milk samples collected randomly at weekly intervals where the
first (300) samples were from the individual milk cans (50 Kg each) at the
producers homes in Abu-Ghraib village while the second (300) samples were
collected from the scale bulk tanks (2 tons) inside the milk reception and
collection center at the same village.
In addition to that (200) swabs were taken from four different location (
Synergistic action of probiotics and prebiotics (synbiotic) has been suggested to be more effective than the two separate components in the prevention and treatment of many intestinal and immune diseases. The present study aimed to examine the anti-inflammatory role of Fenugreek as synbiotic with Lactobacillus acidophilus against Escherichia coli. Twenty four adult males of Wister rats aged 3-4 months and weighted 200-250 gm were used and divided into 4 groups: 1st and 2nd groups were negative and positive control (C and C++) fed with basal diet, the 3rd group (T1) fed diet with Fenugreek seeds (5%) and the 4th group (T2) fed with the synbiotic Fenugreek seeds (5%) and L. acidophilus (5 × 108 CFU/ml) for 45 days. After that, rats in the
... Show MorePresent experimental was carried out on 22 does 2-4 years old. The aim was to induction of estrous in non-breeding season using impregnated sponge with 20 mg of medroxy progesterone acetate (MPA) for 13 days and with an i/m injection of 500 IU Pregnant Mare Serum Gonadotropin (PMSG) hormone 24 hrs before sponge withdrawal and to compare the pregnancy rate using three different methods of insemination; natural , cervical and laparoscopic artificial insemination at fixed time 24-48 hrs after estrous onset. All does were showed signs of estrous (100%), the estrous time was 46.9±4.90hrs (24-60hrs) after sponge withdrawal. While estrous length was 37.09±1.91 (24–72hrs). All experimental animals followed up by abdominal palpation, ultrason
... Show MoreCryptosporidiosis is one of the main causes of economical losses in calf breeding farms due to the diarrhea which results from the infection with this parasite. Diagnostic methods for detection of this infection vary in their efficiency. This study aimed to investigate the prevalence of cryptosporidial infection in Al-Nasr station and compare the efficiency of ELISA in detection of cryptosporidial oocysts with some traditional laboratory methods. A total of 422 faecal samples were collected from three groups of 1-30 day-old calves of both sexes during the period from November 2009 to April 2010. The first group was suffering from watery diarrhea , the second group had pasty faeces, while the third group was normal calves. The first 92 sa
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The present work was carried out in poultry experiment farm of Animal Resources Dept.., College of Agric., Baghdad University, Iraq. Five different levels of single cell protein (0, 5, 10, 15, 20%) replaced to soybean meal in the diets of broiler chick and 600 one day old chicks (Hubberd). The birds were randomly distributed among five treatments (two replicate for each treatment.), 60 chicks per replicate. The results showed that. the diet containing 10% single cell protein was the best diet among the other treatments. The average body weight in six weeks old were 1081.5, 1097, 1171, 1095, 332 gr for the treatments used respectively.
Eighteen rats were divided into three equal groups. The first group was closed orally with quassin, the second group was dosed with quassin after the gut flora were suppressed by difierent antibiotics, and the third group was served as a control. Food intake, water intake, and change in body weight were measured daily before dosing, during two weeks of dosing, and during one week after stopping dosing. Two eats from each group were killed at the end of each week, and stomach, liver, and kidney were collected for histopathologic examination.
The results show a significant decline in daily food intake and daily change in body weight, and a significant increase in daily water intake in both dosed groups during the dosing period
... Show MoreA sample of (700) seven hundred out of (3000) three thousand randomly col
... Show MoreSixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq for molecular evaluation. The extracted nucleic acid was amplified using reverse transcriptase polymerase chain reaction (RT-PCR) targeting genes of segment A (Vp2, Vp3, Vp4 and Vp5 genes) and segment B (VP1 genes). The products of amplification were sent to Korea for sequencing using Sanger method. The sequencing analysis of the IBDV from the Iraqi isolates revealed that each gene had different transition and transversion (nonsense and missense of point mutation) compared to reference genes. The phylogenetic tree analysis showed that the VP2
... Show MoreThe present study aimed to synthesize selenium nanoparticles (SeNPs) using aqueous extract of black currant as a reducing agent. The green synthesized black currant selenium nanoparticles (BCSeNPs) were identified by color change. The characterization of SeNPs was achieved by Ultraviolet-visible (UV–VIS) spectroscopy, scanning electron microscopy (SEM), X–ray diffraction analysis (XRD), and Fourier transform infrared spectroscopy (FTIR). These tests were used to detect: stability, morphology, size, crystalline nature, and functional groups present on the surface of BCSeNPs. The results revealed appearance of the brick-red color indicating the specific color of selenium nanoparticles, and UV-Vis spectroscopy showed band absorbance at
... Show MoreCalotropis procera is announced to be medicinal and poisonous plant to human and animals. In this study, the toxicological effects of aqueous leaves extract were evaluated experimentally in rabbits. The median lethal dose (LD50) was estimated at 2435.25 mg/kg BW using the Up and Down method. Twenty-five local breed rabbits, 1-2-year-old and 1-1.9 kg BW, were divided randomly into five groups with five rabbits each. Those of groupsI, II, III and IV were exposed to 1/10 of LD50 (243.5mg/kg), 1/12.5 of LD50 (194.8 mg/kg), 1/15 of LD50 (162.4 mg/kg), and 1/20 of LD50 (121.8 mg/kg BW of extract respectively for 8 weeks, while those in G.V were left without exposed as a control group. Alkaloids, saponins, tannins,
... Show MoreThe present study was conducted to evaluate the effect of scaffold fabricated from Nano crab shell and Al-kharit (Papyrus Vaccine) for enhancing the healing of the experimentally induced bone defect in dogs. For this purpose, twenty healthy adult mongrel dogs were used in this study which divided randomly into two equal groups, under general anesthesia, 1 cm bone gap was created in the distal part of the tibia, that fixed by bone plate and screws. Nano crab shell scaffold was implanted. All experimental animals showed normal situation without any infection at the site of operation, while the radiography showed a periosteal and endosteal reaction. Moreover, the gaps were bridged faster in the treated group as compared with the control gro
... Show MoreLysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme
... Show MoreListeria monocytogenes has important medical health infection in human and animals. The present study aimed to investigate the significant ultrastructure changes occurred in liver post-infection by pathogenic strain of Listeria monocytogenes. Twenty-six mice of both sexes divided into two groups; 1st group (n=21) infected with 0.2 ml (2x109 CFU/ml) of Listeria monocytogenes I/P and 2nd group (n=5) served as negative control group, all animal of both groups sacrificed at 2, 4, 6 and 24 hours post infection (7 mice for each time of infected group). The histopathology of liver tissues post infection revealed degenerative changes and severe necrosis in hepatic lob
... Show MoreThe present study was aimed to check the amount of variation in some hematologic and biochemical parameters accompanied with natural acquired canine dirofilariasis. Blood samples were collected from sixty five stray dogs (5-10) years old belong to local breed dog in the villages of Al-Hindya area/ Karbala Governorate, The affected animal showed differences in hematological and biochemical values as compared with reference ranges. In conclusion, the disease showed no significant clinical signs although many pathological changes in some blood constituent with serum biochemical parameters were observed, before euthanasia for dogs.
In the present study , we investigate the effect of Gamma radiation of
... Show MoreOut of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty
... Show MoreThis study was conducted on 790 stool samples from infants and young children up to ten yea
... Show MoreIn regard to broiler diseases in Sulaimani district, different factors like
environmental temperature, humidity, wind velocity, the source of hatchery eggs
and day-old chicks were studied. The study involved the history, some
concurrent diseases, methods of protection against viral diseases, clinical signs,
post mortem lesions, isolation and identification methods (cultural and
biochemical) and sensitivity test. The 4th and 5th weeks ages were the
exposed ages for respiratory diseases, precisely Escherichia coli infection. The
temperature factor (high and low) was among the most prominent predisposing
factors, since 39% and 30% of E.coli isolates obtained through winter and
summer seasons successively. The low