The objective of present work is to estimated peripheral blood serum concentrations of pregnancy
hormones, oestradiol , progesterone and testosterone , in cows . For this purpose 24 Frezain- Holstein
cows at different stages of pregnancy the blood samples were taken from jugular veins. The serum
were separated and frozen at – 20 c until analysis. The serum hormones were measured by a specific
ELISA technique (ELISA Linear Multi Reader). The data were represented Mean + S.D. Progesterone
was high during pregnancy reaching a maximum of 91.94 + 26.09 ng/ml during last thirds (6-8
months) of pregnancy , but was below 9.12 + 2.41 ng/ml for several months during the pregnancy.
Oestradiol levels varied from 9.0+ 2.89 pg/m
A total number of 600 fecal samples were collected from patients attaining general Abu-
Ghraib hospital during the period from 1st march 2006 till 28th February 2007. The samples were
examined for detection of intestinal protozoan Entamoeba(E).histolytica infection by direct smear with
Iodine stain and flotation technic using Zinc sulphat solution .The overall rate of infection was
32.5%.Asignificant differences were observed in the rates of infection between males and females
which constituted 46.44%, 25.64% respectively. All age groups were infected, the highest rate of
infection 61.9%, 29.6% were noticed in males and females of 2-5 years old respectively, whereas the
lowest rates 15.15%, 10% were recorded at the a
This study was conducted to investigate the effect of probiotic prepared from Lactobacillus acidophilus & used
as immunostimulator to improve the humeral &cellular immune response in lambs vaccinated with the Rev-1 vaccine.
Lactobacillus isolated from intestinal contents of sheep after culturing on specific media &study the morphological &
biochemical characteristic Lactobacillus acidophilus was selected as probiotic after definitive diagnosis and depending
on their higher adherence ability to epithelial cells of the intestine and have high tolerance to low pH and bile salts .then
sensitivity test of the strain against antibiotics was done The experimental study included 15 lambs (age 5-8 months)
were eq
To investigate the prevalence of cryptosporidiosis among neonatal lambs and sheep in different areas
of Baghdad include (Alshaab, Alshoala ,Albayaa, Alalawy, Madenat alsader, Aldorah, Alhosaenea,
Algazalea, Alrashdea) ,also the study investigated effect of sex ,age, season on the infected rate from
December 2007 till August 2008.
The laboratory methods were used to identified the oocysts :Modifide Ziehl Neelsen stain and flotation
with Shether’s solution .
A total of 500 fecal samples were collected from animals at one day -two years old lambs and sheep,
males and females
All fecal samples obtained from three groups of animals state: diarrhea , pasty and normal.
The study recorded total rate of cryptosporidio
This study has been conducted to study the effect of feed withdrawal (8 hours after slaughter) and
adding dried bakery yeast on dressing percentage and meat chemical 45 marketed broiler chickens at 8
weeks of age .The birds randomly distributed into three treatments (5 birds ) and each treatment to
three replicates (5 birds per replicate ) which were: un-treated control (T1) , broiler chickens subjected
to feed withdrawal 8 hrs. before slautered (T2) , broiler chickens fed a diet contained 1% of dried
bakery yeast three days before subjected to feed withdrawal 8 hrs. before slaughtered (T3) . The data
obtained revealed the following :-
Adding dried bakery yeast three days before subjected to feed withdrawal 8 hrs. be
In order to investigate the presence of Listeria monocytogenes in raw and imported Ultra Heat Treatment
(UHT) milk, this study was done by random collection of samples from different areas in Baghdad province and
its surroundings from different animals (small and large ruminants) as 68 total samples (53 raw milk samples and
15 imported UHT samples)collected weekly from October 2007 till January 2008, and processed according to
standard protocols of Listeria monocytogenes .
The results showed reisolation of 10 isolates from 68 samples (14.7%) as 6 isolated from raw milk samples
(11.3%) and 4 isolated from UHT milk samples (26.7%) ,these revealed significant differences (P≤0.05) in
isolated percentage of Listeria mon
This experiment was carried out at poultry house, college of Veterinary Medicine, Baghdad university,
Which lasted 42 days, to by adding Thymus vulgaris the used part (Leaves, Steams and Flowers) and
Cinnamomum zeylanicum the used part (bark) to the concentrate diet for the experimental period for 6 weeks.
The study was aimed to find out the effect of thyme or cinnamon on productive, physiological blood traits of
broilers by using 150 unsexing Arbor Acres chicks at the age of one day. These chicks were randomly divided
into five group equally (30 chicks each). Each group was subdivided into two equal subgroup. And fed on the
following ration.
1-The first group was fed on ordinary diet kept as a control group.
2- T
To identify the bacterial isolation from dogs have cancer diseases, the samples were (throat swab and urine) have
been taken from (64) dogs suffering from different cancer diseases includes (38) females and (26) males.
The result of throat swab as were follows: E.coli (50%), Actenomyces pyogenes (40.6 %) , Beta Heamolytic
Streptococcus group D (21.8 %) , Staphylococcus aureus (21.8 %), Klebsiella spp also Actenomyces viscosus
(18.7%), S.epidermidis(12.5%), Beta Heamolytic Streptococcus groupG (9.3 %) , Proteus mirabilis (9.3 %) ,
Beta Heamolytic Streptococcus group B (6.2 %) , Bacillus subtilis (6.2 %), Micrococcus (6.2%) ,
Pseudomonas aerugenosa also Proteus vulgaris (3.1%).
While the urine samples of infected doges
It is well known that monotherapy does not provide therapeutic response in all hypertensive. Some
patients show an excellent response, while in others there is a poor response. Combination
antihypertensive therapy is administered when blood pressure is inadequately controlled by
monotherapy to achieve a balanced and additive antihypertensive effect with minimum adverse effects.
Both angiotensin converting enzyme (ACE) inhibitors and dihydropyridine type of calcium antagonists
are well established and widely used in monotherapy. An understanding of differences in the
mechanism of action of these agents allows a logical approach for the use of these agents as a
combination therapy. This study was designed to evaluate t
Gossypol is a substance found in the cottonseed. Recently in Iraq and in other countries the cottonseed
was used as a concentrate for fish and other animals without awareness of the risk. The present study is
designed to shed the light on the deleterious effect of gossypol on some hematological parameters and
enzymes of male common carp Cyprinus carpio l.. Oral administration of gossypol 20 mg./kg. b.w. for
ten days was found to be a strong lyses for red blood cell, reduced packed cell volume and increased
erythrocyte sedimentation rate and decreased total white blood cell. Furthermore alkaline
phosphotase, alinine aminotransferase and aspartate aminotransferase were increased which reflect
liver and kidneys damage c
Groups of mice experimentally infected with ringworm Trichophyton mentagrophyte were treated with
ointments prepared from 5% cold alcoholic extract of two plants of Thymbra spicata , Ruta chalepnsis
and compared with the commercial drug “Clotrmazole” (1%), recovery from ringworm needed 16 , 18 ,
14 days for Th.spicata, Ruta chalepensis and Coltrmazole respectively.
On the other hand 28 naturally infected calves with ringworm from the genera Trichophyton
mentagrophytes and Aspergillus were divided into four groups, and each group treated individually with
one of the ointments 5% concentration , cold alcoholic extract of Th.spicata and Ruta chalepensis beside
“Clotrimazole”.The fourth group was left as a positi
The aim of this study to mentioned the effect of pregnancy on blood parasitemia of some protozoal
infections and total, differential of white blood cells counts from 22 Awassi ewes 3-3.5 years age
naturally infected by Theileria hirci and Anaplasma marginale.
Our results showed that there was a positive relationship between the development of pregnancy stages
and the blood parasitemia till reach the highest levels during the last months (fourth and fifth) of
pregnancy of Theileria hirci and Anaplasma marginale 3±0.06%, 2.29±1% and 0.77±0.15%,
1.09±0.87% respectively.
The total white blood cells count decrease in the first month of pregnancy (5.780±0.54×10³
cell/mm³), while increased during the others which
This study was conducted to evaluate the effect of body condition score (BCS) and supplementary feeding
on the reproductive performance of Awassi ewes.
One hundred sixty Awassi ewes aged 3-5 years (Average live weight 47.41±0.76) were used in a
completely randomized design. Six weeks before mating ewes were screened for their body condition
score (BCS) and divided into two groups, low BCS (<2.0) and high BCS (>2.0) and ewes in each group
were subdivided according to BCS and to non-supplemented and supplemented groups (n=40 ewes
).Therefore, the four groups were:
1. Group (LBCN) = Low Body Condition Non-supplemented.
2. Group (LBCS) = Low Body Condition Supplemented.
3. Group (HBCN) = High Body Condition
The poultry industry faces increasing challenges from heat stress caused by global warming, particularly in tropical regions. Heat stress, caused by rising environmental temperatures, undermines the health and productivity of poultry, resulting in significant economic losses. This review comprehensively addresses the physiological and behavioural responses of poultry to heat stress and explores the key mitigation strategies, categorized under genetics, management and feeding. Genetic approaches, like utilizing specific genes in breeding, show promise but require wider adoption. Management practices such as housing design, including orientation, insulation and ventilation, controlled lighting and thermal manipulation are critical to maint
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Heavy meals are widely known as environmental pollutants due to their toxicity, heat stability, long staying in the atmosphere, and their capability to accumulate in the body. The current experiment investigates the concentration of lead (Pb) and cadmium (Cd), which are considered heavy metals in the liver and meat of fresh and imported frozen chicken, by studying the effect of cooking process using the boiling method. A total of 80 samples were randomly collected and examined: 40 frozen (10 from each of the liver and meat) before and after cooking) and 40 fresh (10 from each the liver and meat) before and after cooking) from different regions in AL-Karkh Province, Baghdad. Atomic Absorption Spectrometry device |
Conventional ocular drug delivery forms (eye drops, eye ointments, and eye gels) possess poor bioavailability, less retention, and rapid precorneal discharge. In veterinary medicine, treating eye infections in animals like rabbits, dogs, and cats poses similar challenges. An in-situ gel drug delivery system (ISG-DDS) provides sustained action with a low formulation cost, which can be advantageous for veterinary applications. This study developed and characterised a pH-responsive ophthalmic ISG of levofloxacin and tinidazole with the potential to be used in both human and veterinary medicine. Carboxypol 980 (CBL-980) was used to make the ISG gel, and hydroxypropyl cellulose (HPC) changed the viscosity. Nine formu |
Our objective in this work was to estimate normal values of biochemical parameters in laboratory animals in Iraq. Values were pooled from studies conducted in Iraqi universities using a meta-analysis approach. Pertinent peer-reviewed published studies were retrieved from the Iraqi Academic Scientific Journals (IASJ) database. Random-effects inverse-variance model was used to obtain pooled means of the parameters from the set of studies qualified for the analysis. Among a total of 460 records identified from IASJ, 264 records were eligible for the statistical analysis, which reported 102 values for different biochemical parameters. The mean of the sample size for normal animals that was used in the studies includ |
Domestic cats (Felis catus) pose a significant challenge for pet owners during the mating season, necessitating effective contraceptive strategies to manage feline populations. This study investigated the use of medroxyprogesterone acetate (MPA), precisely the human formulation Depo-Provera®, as a potential solution for feline contraception. Ten adult female cats were included in a randomized controlled trial, with the treatment group receiving a single intramuscular injection of 25 mg/cat MPA. Clinical observations, vaginal cytology, and hormonal analysis were conducted to assess treatment efficacy. Results demonstrated that MPA treatment effectively suppresses estrus behaviours, prolongs interestru |
This study aimed to assess the occurrence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) infections in fertile eggs using Enzyme-linked Immunosorbent Assay (ELISA) and Real-Time PCR. A total of 62 yolk samples were collected from August 2022 to April 2023. Of these, 31 samples (50%) were tested positive for antibodies via ELISA, while 13 samples (21%) were positive using Real-Time PCR. Specifically, 10 samples (16.12%) were positive for MG, and 3 samples (4.83%) were positive for MS, indicating a higher incidence of MG than MS in the hatcheries examined. The results suggest that maternal antibodies detected by ELISA may confer protection to chicks against MG and MS. This study confirms the vertical transm
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Streptococcus pneumoniae are common bacterial pathogens that can cause a range of diseases in humans and animals. This study attempts to clarify the course and consequences of pneumonia caused by this bacterial infection. A total of 6 male un-weaned lambs aged between 1 to 2 months and weighing 5 to 7 kg were subjected to S. pneumoniae strain ATCC 6303 serotype 3 at 2×106 CFU/mL by inhalation to induce pneumonia. Pneumonic clinical signs were monitored daily throughout the study period. The blood samples were collected from all animal groups at day zero before pneumoniae induction and 3, 6, and 14 days post infection for total and differential white blood cell count (W |
This study evaluated the efficacy of β-glucan, extracted from Candida albicans isolated from mastitic goat milk using an alkaline-acidic method, in treating C. albicans-induced mastitis. Twenty lactating goats (2–4 years old, 25–30 kg) were randomly divided into five groups (Group I–Group V), with each group consisting of four goats. Sixteen goats were intramammarily inoculated with 1.2 × 10⁷ yeast/2 mL of virulent C. albicans. The groups were as follows: Group I (negative control, uninfected), Group II (positive control, infected but untreated), Group III (treated with Nystatin, 200,000 units/half udder/day intramammary for 7 days), Group IV (treated with β-glucan, 5 mg/mL/half udde |
The lack of effective treatment for oral mucosal ulcers has motivated clinicians to search for other therapeutic techniques to improve oral ulcer healing. Applying fenugreek to an ulcer triggers the release of its anti-inflammatory components, that might reduce any superfluous inflammatory processes and accelerate wound healing. The aim of this study was to investigate the effect of fenugreek leaf oil on the ulcer associated with the expression of epidermal growth factor receptor (EGFR). A total of 24 adult male rats weighing between 350 and 450 grams and 4-6 months of age were used in this study. The experimental design included: the normal group (no ulcer), the control group (12 ulcers left without treatment t |
Type 2 diabetes mellitus (T2DM) in dogs is a complex, multifactorial disease that is characterized by chronic hyperglycemia and insulin resistance. Current therapeutic options are often limited by side effects and variable efficacy, highlighting the need for more effective and safer treatments. This study assessed the therapeutic potential of ginger ethanolic extract (GEE), GEE-loaded chitosan nanoparticles (GEE-CNPs), and chitosan nanoparticles (CNPs) against T2DM in dogs. Twenty adult local breed mongrel dogs of both sexes, aged 7 to 13 months, with an average body weight of 10.4±0.76 kg, were included. The dogs were allocated into five groups (n=4 each): a non-diabetic, untreated Nega |
Canine mammary tumors (CMTs) are frequently occurring types of tumor in female dogs. Matrix metallopeptidase 9 (MMP-9) plays a role in dismantling the extracellular matrix during normal bodily functions and diseases, including cancer. The goal of this research was to evaluate the serum levels of MMP-9 as a potential diagnostic indicator for CMTs using the ELISA method. We collected tissue samples from mammary glands and blood specimens from 50 dogs suspected of having CMTs and 30 healthy control dogs. Histological examination was used to diagnose the mammary tumors, with findings indicating that 88% of the cases were malignant CMT and 12% were benign. According to ELISA results, there was a statistically conside |
Growth plate injury is one of the most significant sequelae that affect the animal and man life in adulthood. This condition considered as one of the real problem encountered by many orthopedics which can result in the formation of a bony bridge leading ultimately to limb length discrepancy and angular deformity of the long bones in kid animals. The objective of this study was to evaluate the deformities of the tibial length and angle radiographically after experimentally inducing growth plate injury. Five local breed kit rabbits included in this study, had body weight and age ranges of 500 to 1100 g and 6 to12 weeks, respectively. The animals were exposed to approximately 5×5×1 mm injury in the proximal tib |
The present study was undertaken to evaluate the use of ionizing radiation in decontaminating the
total microbial burden of Capsicum peppers (Capsium annuum), Caraway (Carum carvi), and Clove
(Dianthus caryphyllus). The spices were irradiated in polyethylene bags with 5KGy and 10KGy of
gamma radiation from Co-60 irradiator. Total number of bacteria and molds survived per gram of
spices after irradiation were calculated. Results showed that 5KGy gamma radiation reduced the total
number of bacteria by 95-98% and the number of molds and yeast by 62.8-98.2%, while 10KGy
reduced the total number of bacteria by 95.5-99.9% and the number of the molds and yeast by 67-
99.7%. These percentages are accepted within the maximum
This experiment was carried out to evaluate the role of flavonoinds of Nigella sativum seeds to reduce the
effect of hydrogen peroxide on electrocardiography in rabbits. Eighteen adult male rabbits were divided into
three equal groups (6\group) and were treated for six weeks as follows: first group (control), which were given
ordinary tap water, second treated group (T1), animals in this group given 0.5%H2O2 in drinking water and
third treated group (T2) was handled as in T1 plus oral intubation of flavonoinds (27.5mg\Kg B.W).
Electrocardiography was recordings from rabbits after 3 and 6 weeks of treatment. The results conducted that
H2O2 treated group (T1) showed significant increased in P-R interval, R-voltage and sig
Experiment study was done on the pathogenicity of Salmonella give infections in dogs . The
experiment was carried out on 8 puppies ranging in age from 8-9 weeks. The puppies were divided randomly
into two equal groups. Group I inoculated orally with 4.8x109 Salmonella give and group II saved as control.
The disease was characterized by acute gastroenteric and septicemic forms with variable severity. The most
consistent clinical signs were anorexia, fever, diarrhea, vomiting repression and recumbancy. Salmonella was
consistently isolated from blood as well as from feces. Gross pathological changes and histological
examination were characterized by sever congestion and inflammatory cells in the most of GIT
The aim of this study was the assessment of pentoxifylline (PTX) effects on female reproductive
hormones as well the body and reproductive organs. Sixty white mice with approximately same ages
(eight) weeks and body weights were randomly divided into (six.) equal groups 10 mice for each group.
Group 1: Received tap water along the period of experiment and considered as a control group. Animals
of group 2,3,4,5 and 6 were treated with 16 mg (PTX) / Kg B.W. daily for 2,4,6,8 and 10 weeks
respectively. Before and after treatment all animals were weighed, and blood samples were taken for
follicle stimulating hormone (FSH), lutenizing hormone (LH) and Estradiol (E2) hormones analysis.
After post – Mortem examination rep
Hematological changes in experimentally infected dogs with Salmonella give were studied in 8
puppies aged 8-9 weeks. They were separated equally into two groups. The first group has been infected with 4.8x
CFU with Salmonella give , while the second group was used as control. Hematological changes were 9 10
characterized by an increase in P.C.V. , Hb , 'RBC count ,TPP and fibrinogen , while there was no significant
changes in MCV ,MCH , MCHC . The total leukocytic count was increased during the two days and then gradually
decreased at the end of the experiment