Mammary adherent cells (MAC-T) were infected with six isolates of Streptococcus uberis (S. uberis). Three isolates were cases of mastitis in dairy cows and belonged to clonal complex 5, 143, which is associated with virulence and three were from cows with no clinical or laboratory evidence of mastitis. All these isolates belonged to clonal complex 86 which contains strains of low virulence. After incubation at 37oC for 24 h, there were no significant differences in the number of adherent or internalized S. uberis between mastitis (M) and non- mastitis (NM) isolates (p> 0.05).
The levels of tumour necrosis factor (TNF-α), measured in treated MAC-T cells supernatant with S. uberis by ELISA, were significantly elevated in cultures in
The objective of the present study was to determine the influence of prolactin hormone on reproductive activity of female rabbit. Study was conducted on the laboratory animal house and it depended on fifteen mature rabbit does and one mature buck housed on a special cage separately. The females were divided randomly into two groups; the first group is Control group: Female of this group (n=7) were administrated 2 ml of normal saline orally /day. second group is treated group: Females of this group (n=8) were received orally 5 mg of Parlodil® (Bromocriptine) daily for 5 consecutive days then stopped one week and repeated in the same schedule. All animals were clinically observed for detect any drug reaction and signs of sexual behavior.
... Show MoreThis study aimed to investigate the protective influence of olive leave extract zinc oxide nanoparticles (OLEZnONPs) complex against gentamicin–induced kidney dysfunctions in goats. Twenty five adult female goats were randomly divided into five equal groups and treated as follows: control group (C) administered sterile distilled water (IM) for 10 days, group G administered 25 mg/kg BW gentamicin (IM) for 7 days, group Z administered 10 ìg/kg BW of OLEZnONPs (IP) for 3 days, group GTZ administered 25 mg/kg BW gentamicin (IM) for 7 days and then 10 ìg/kg BW of OLEZnONPs (IP) for 3 days, group GWZ administered 25 mg/kg BWs gentamicin (IM) and 10 ìg/kg BW of OLEZnONPs (IP) together for first 3 days and then
... Show MoreThe effects of oseltamivir administration, an anti influenza viruses A and B, on some functional parameters of rat liver were investigated, to evaluate the possible hepatotoxic effect. Eighteen (18) wister male albino rats with body weight ranged 150-190 gm were divided into three groups, the first group(T1) was treated orally with 1mg/kg.BW as therapeutic dose of Oseltamivir for 7consuctive days. The second group (T2) was treated with the same dose for six weeks, while the control group dosed distill water.The results revealed, there was a significant increase in the onset of barbiturate sleeping time and a significant p ≤ 0.05 decrease of the duration of barbiturate sleeping time of the T2 rats . The liver enzymes activity revealed a
... Show MoreIn the present study, the immunomodulatory effect of Neem (Azadirachtaindica)
seed aqueous, ethanolic extracts and Candida albicans cell wall mannoproteins on the
immune response of mice vaccinated with Brucella Rev-1 vaccine was investigated. The
study was conducted on two main groups(160 mice for each group), Each group was
divided into eight subgroups 20 mice for each (I: treated with distilled water, II: treated
with the Brucella Rev-1 vaccine, III: treated with mannoproteins, IV: treated with neem
aqueous extract, V: treated with neemethanolic extract, VI, VII and VIII: treated with
mannoproteins, neem aqueous extract and neemethanolic extract, respectively, then they
were vaccinated with Brucella Rev-1). All
The in vitro sensitivity testing of mastitis pathogens, associated with udder infections in cows,
... Show MoreBacteriological and histopathological investigations were conducted on ovine and caprine aborted foeti, ten each,
... Show MoreOut of 210 tissue samples (lung and lymph nodes ) collected from buffaloes examined by ELISA specific to(BVDV) Bovine Viral Diarrhea Virus antigen ten (10) samples were positive out of 160 lung tissue collected with (4.7%)and (3)positive sample were detected out of 50 lymph node tissue samples collected with (1.4%). Totally 13 positive samples with (6.1%) in buffaloes examined. The total positive tissue samples were divided to three age group. Group age one from (birth -6 month), group age two from (6-18 month) and group age three (18 month up). The positive samples were (1),(10) and (2) with (0.47%),(4.7%)and (0.95%)respectively that indicate the presence of BVD antigen in buffaloes (Bubals bubalis) .Out of (210) sera samples collected
... Show MoreThe study included 300 unsexed broiler chicks (Hubbard) were used to evaluate (Roselle Flower) as a feed additives on the productive performance of broiler. The chicks were divided into five groups with three replicates (20 birds in each) and fed on five diets. Diet 1 (control group) dried Roselle flower powder were supplemented to the diet 2, 3, 4 and 5 as 0.25, 0.5, 0.75 and 1% respectively. The result revealed that a significant (P<0.05) increase in body weight, body weight gain, feed consumption and improvement in feed conversion ratio. Although the differences between groups for edible parts percent were not significant (Heart, Liver and Gizzard). The hematological values PCV%, RBC, WBC and H/L ratio were increased significantly
... Show MoreThe main objective of this study was to evaluate raw milk quality. This study was designed
... Show MoreThis study was carried out to investigate 87 lungs of camel slaughtered in Al-Najaf
... Show MoreTwenty weeks old Euribrid-Hisex Brown laying hens were used to study the effect of water po
... Show MoreThe globule leukecytes and mast cells were detected in the respiratory tract of camel with natural
... Show MoreAttenuated 3rd stage larvae of Dictyocaulus filaria, by continuos em
... Show MoreTestes from 700 male goats aged l - 3 years , slaughtered at Baghdad abatteir, were examined during 6 months period. Histological and bacteriological examinations were done when gross lesions were observed. The results revealed that epidedimytis was observed in 21 (3%) of the examined animals ; 16 (2.3%) cases were unilaterally affected and S (0.7%) cases were bilaterally affected.
Bacteriological isolations from infected organs included :
Corynebacterium ovis (6 isolates) and Actinomyces pyogenes (3 isolates) both constituted the majonty of isolates ; as well as E. coli (4 iso-lates), and Pseudomonas aeruginosa , Staphylococcus epidermidis, Cory. bovis., Staph. aureus, Cory , ulcerams &. Yersinia pseuclotuberculosis
... Show MoreThis study aims to throw light on the harmful effect of kerosene combustion on the respiratory sy
... Show MoreThis experiment aimed to study the effect of diet supplementation with
feed enzyme (Poultrygrow) or chromium yeast (Biochrom) on the intestinal
microbial balance of broiler chicks. A total of 300 broiler chicks, one day old,
were randomly allocated into three treatment groups and named T1, T2 and T3
respectively. Chicks in T1 used as control while chicks in T2 and T3 were feed
diets with two feed additives, Poultrygrow and Biochrom, respectively.
The data show that the two feed additives were decreased the total
bacterial count and coliform count in crop, Jujinum and large intestine but the
statistical analysis showed that the effect were not significant. Supplementation
of diet with Biochrom were significantly
The aim of this study to detect Cryptospordiosis among children (1 day –10 years) in some Wadi Al-Shati areas (Brack ,Akar,Al-Qurda and
Wanzreek). A total of 280 faecal samples were examined by flotation with sheather s’ sugar solution and acid fast staining techniques to detect Cryptospordium oocysts .The results of study demonstrated that 29 samples (10 .3 % ) were positive with highest infection rates in young children between ( days and 1-2
years, which reached 34.2 % and 23 % respectively.
This study was conducted at the College of Veterinary Medicine ,University of Baghdad to prepare synbiotic of Sacch. cerevisiae and used as 0.1 and 0 .2 % of feed (T2,T3) for reducing the experimental infection of Salmonella typhimurium to broiler chicks ( 1-15 days) by using 40 birds
divided into four treatments , T1 was negative control with 0.1 % synbiotics and T4 was positive control with experimental infection only , production and blood parameters were studed , and the results showed the following: A synbiotic of Sacch. cerevisiae was prepared at the first time locally which have 10 7 cfu of Sacch. cerevisiae and 10 mg Manna
... Show MoreTwo hundreds suspected typhoid fever patients blood and stool samples
were collected. The diagnosis of patients was concerned with the clinical
symptoms and serologically obtained from Widal test by using both H,Oantigens.
The titer of antibodies were concentrated at a levels 80, 160 against
each antigens. Typhoid fever infect both sex, but the rate of infection among
males was higher (54%) as compared with that of females (46%), while the
highest rate of infection was encountered among the age 15-29 years old in
males and females 24.5%, 17.5% respectively. Sixty one isolates of
Salmonella typhi were isolated from the blood and feces specimens (49,12)
respectively. Serological characterization of isolates showe
To investigate the impact of Enterococcus faecium as a probiotic in the
performance of layer male chicks and some aspects of immunity, Newcastle disease
antibodies and the blood picture, this bacteria was given in the feed of layer male
chicks with a dose rate of 1.5 g\kg of feed, each kg of feed contained (5×1011CFU)
along the experiment which last 57 days. 150 male chicks were divided into five
groups, the 1st group was given the probiotic in the feed, the 2nd group was given the
probiotic and was exposed to bad ventilation and high temperature as a stress, the
third group was given the probiotic and chlortetracycline in the feed, the fourth group
was given the probiotic and then infected with E. coli on day 21
The influence of heat and cold on the L.monocytogenes in the milk
In order to know the thermal resistance of L.monocytogenesin the
contaminated milk, 40gram of powder milk was dissolved in 400ml
of heated water.The milk solution was sealed in 40 test tubes 10 ml
in each one.these test tubes were divided into 6 groups ,10test tubes
in each the 1st,2nd group &5 test tubes in each the 3rd ,4th ,5th ,6th
groups,then all the test tubes were inoculated with
L.monocytogenes &incubation for 24hrs at 37c.the samples of the
1st &2nd group were dipped in awater bath at tempreture 63c for
30minute &72c for 1-5.5 minute respectively .also the samples of
3rd &4th groups were dipped in awater bath at 72
This study was concerned on the preparation of several antigens on different
types of culture media like brain heart infusion, tryptic soy agar and broth. A
comparison was carried out among the prepared antigens using skin test in non-
vaccinated guinea pigs which showed negative results. Another trials were conducted using sensitized guinea pigs with live attenuated anthrax vaccine. After three weeks of immunization, skin test was conducted and all antigens revealed positive skin reactions. The highest means were recorded by the dilution 1/40 on the brain heart infusion agar with the mean of erythema of 16.38 mm and a difference of thickness 3.44 mm, while on tryptic soy broth the mean of erythema wa
... Show MoreThe aim of this study was carried out to demonstrate the cross-immunity
between Nocardia asteroids and Salmonella typhimurium antigens in mice.
18 mice, from both sex,were randomally divided into three groups equally.
the first group immunized subcutaneously with 0.0 5 micrometer / animal of
Nocardia asteroids antigen and abooster dose of the same antigen was
injected after week of the first dose. The second group was treated as the
first group with Salmonella typhimurium antigen. .The third group was
served as non-immunized control group. Delayed hypersensitivity and tube
agglutination test were checked at 30 days post-immunization. By using
Nocardin and Sallmonelin antigens.
The results revealed that the a
This study has been conducted to study the effect of experimentally
infected broiler chicks orally by two dosages of Listeria monocytogenes in
the percentages of serum protein of 30 broiler chicks at 7 and 15 days of
infection . The birds randomly distributed into three treatments , T1 : control
group , T2 : chicks orally infected by 6 X 405 CFU of L. monocytogenes , T3
: chicks orally infected by 34 X 4010 CFU of L. monocytogenes. The data
obtained revealed the following :-
Percentages of serum Pre-albumin , Albumin , Total albumins and -
Globulin were decreased significantly (P<0.05) , in the same time
Post_albumin , , - Globuluins and Total globulins increased significantly
(P<0.05) in b
Data analyzed included 13838 records belonged to 4100 Holstein cows over period 1988 to 2005, at the Nasr Dairy Cattle Station. The aim of the study is to evaluate sires genetically according to their daughters breeding efficiency which was estimated by Tomar equation and to estimate heritability and phenotypic trend for breeding efficiency.
General Linear Model within SAS program was used to investigate the effect of some fixed factors on the breeding efficiency. The overall breeding efficiency ratio was 79.91% .Component of variance for ra
... Show MoreMastitis were diagnosed in (80) ewes depending on clinical examination , California mastitis test , & bacterial isolates inclu
... Show More
This study was performed to investigate the effect of lead exposure on resistance of mice immunized against haemorrhagic septicaemia and challenged with virulent organisms. Lead treated mice showed greater susceptibility to challenge with P. multucida than control especially if lead nitrate is administered orally. this result showed that treatment with lead initials resistance of mice and might interferes with the vaccination programs. Cadmium chloride (cd) injected into rabbits at various time intervals in relation to tetanus toxoid injection was found to have some effect on the immune response. A reduction in the primary immune response was detected in case if (cd) is given two weeks prior to toxoid injection. On the oth
... Show MoreThis experiment was conducted to compare and estimate the fertilizing capacity of native with New Hampshire chicken semen. Also to study the effect. Of different levels of semen dosage on fertility percentage, duration’ of fertility and duration of fertilizing ability. In this experiment (12) native and (12) N.H. male chicken with (240) Hisex laying hens have been used. The results of the statistical analysis showed that there were no significant (P>0.05) differences between the native and N.H. male chickens for the fertilizing capacity. fertilizing percentage in duration of fertility and fertility duration. The values were 90.40, 90.73%; 85.77, 85.60%; 12.11, 12.3 days for native and N.H. chickens, respectively. There were no signi
... Show MoreSers. from 525 Arabian horses were tested for the presence of antibodies to Toxoplasma gondii by the indirect haemagglutination test. Of these sera, 39(7.43%) were positive. Thirty eight (8.15%) of seropositive animals were horses of two to 13 years of age and only one of the yearlings (1.5 year old) was seropositive. Twenty six of males (6.57%) and 13 cf females (10.08%) were seropositive. There was no significant (P)0.05) difference in the prevalence of toxoplasmosis between males and females.