Escherichia coli is consedered to be one of the normal flora of the human
and animal intestinal tract, but there are some serotypes which are diagnosed
as a pathogenic such as Enteroheamorrhagic E.coli O157 that cause disease
ranging in severity from mild to lethal and it's significant as a major foodborne
pathogen and as a public health problem. The purpose of this study
was to provide the prevalence of E.coli and E.coli O157 in locally produced
soft cheeses, detection and enumeration were carried out using specific
differential media (CT.SMAC,E.M.B.), serological test (Latex O157) and
biochemical test.
A total of 50 samples of locally produced soft cheeses were collected from
supermarket in Baghdad City du

From March to November 2004 a total of 634 stool samples from the children coming to the
hospitals, cure centers and randomly from the children of kindergarten and primary schools. General
stool examination and precipitation methods were performed on each specimen, the infection rate of
Hymenolpes nana 1.8% among children for both Gender(male and female) for different ages that had
been recorded the highest rate for (4- 6)year is 2.94% and the lowest rate for (9-12)year is 1.4%.
Seasonal variation were discussed, that August was recorded the highest rate 5.8%.

Colonies of E.coli O157:H7 were isolated from 35 raw milk sample and their identification were confirmed based on biochemical reactions and both cultural and serological characteristics. Presumptive E.coli O157:H7 isolates obtained by selective plating on both CT-SMAC and Chromogenic agars were further tested serologically for the presence of both O157 and H7 antigenes using the commercial available latex agglutination test kit. The unhygienic practices in the production of milk in Al-Thahab Al- Abiedh , Abu-Graib, Al-Zedan and Khan Dharie were reflected on the highest significant (p<0.01)prevalence level of contamination with E.coli O157:H7 that appeared to be 80%, 80%, 60% and60% respectively. Homogenization pressure of 1000 psi and

The present study was conducted to measure the concentration of progesterone throughout gestation and parturition in control and treated bitches. Thirty anestrus bitches aged 3 - 6 years and weighing 19.5 - 26 kg were used in this study. The bitches were divided equally into three groups as follows: the 1^st group as a control group  without treatment, the 2^nd group as a treated group anestrus bitches treated orally with 0.125 mg/kg BW bromocriptine (Parlodel) twice daily, and the 3^rd group treated orally by 0.012 mg/kg BW cabergoline (Dostinex) until the appearance of estrus signs. The results showed that the number of responded animals were in proestrus bleeding and became pregna

Impact of vitamin E against mercuric chloride (HgCL₂) induced renal toxicity in Wister albino rats was studied. Feeding of the rats with diet and water contaminated with a non lethal dose of the mercuric chloride (20 parts per million) every other day for 42 days resulted in significant increase of serum malondialdehyde (MDA), which is an important biomarker of the oxidoreductive stress, and significant decline in each of the reduced glutathione (r-GSH) concentration, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) enzymes activities, which constitute a vital part of the endogenous protective antioxidant system, as compared to the control group. The study found that the simultaneous

     This study was designed to investigate the effects of the addition of different ratios of sunflower oil to the diet of Awasi ewes on the rumen fermentation and milk composition. It was conducted by using 12 Awassi ewes (2-3.5 years old) with average body weight 56.62 kg. The ewes were randomly divided into three groups. Each group had four ewes according to the age, body weight and milk yield. The ewe in each group received 1.4 kg/ dry matter for three rations consisting of barley, wheat bran, soybean meal and alfalfa hay. The first group was fed on control ration, while 2.5 and 5 % of sunflower oil were included in the rations of the second and third groups, respectively. The results showed that the treatments h

A total number of 600 fecal samples were collected from patients attaining general Abu-
Ghraib hospital during the period from 1st march 2006 till 28th February 2007. The samples were
examined for detection of intestinal protozoan Entamoeba(E).histolytica infection by direct smear with
Iodine stain and flotation technic using Zinc sulphat solution .The overall rate of infection was
32.5%.Asignificant differences were observed in the rates of infection between males and females
which constituted 46.44%, 25.64% respectively. All age groups were infected, the highest rate of
infection 61.9%, 29.6% were noticed in males and females of 2-5 years old respectively, whereas the
lowest rates 15.15%, 10% were recorded at the a

The objective of present work is to estimated peripheral blood serum concentrations of pregnancy
hormones, oestradiol , progesterone and testosterone , in cows . For this purpose 24 Frezain- Holstein
cows at different stages of pregnancy the blood samples were taken from jugular veins. The serum
were separated and frozen at – 20 c until analysis. The serum hormones were measured by a specific
ELISA technique (ELISA Linear Multi Reader). The data were represented Mean + S.D. Progesterone
was high during pregnancy reaching a maximum of 91.94 + 26.09 ng/ml during last thirds (6-8
months) of pregnancy , but was below 9.12 + 2.41 ng/ml for several months during the pregnancy.
Oestradiol levels varied from 9.0+ 2.89 pg/m

شملت الدراسة الحالية 89 مبيضا ، جمعت من مجزره الشعلة مباشرة بعد ذبح الحيوان ،حفضت المبايض في المحلول
ألفسلجي بدرجة حرارة 73 م ونقلت إلى المختبر خلال 4 7 ساعات ، تم قياس قطر الجريبات المتواجدة على المبايض -
وقسمت إلى جريبات كبيرة الحجم ) 01 6 ( مليمتر وصفيره الحجم ) 5 0 (مليمتر . سحبت البويضات المحاطة بالركمه - -
المبيضة بطريقه الشفط من الجريبات الكبيرة والصفيرة بواسطة ابره قياس 09 كيج موصولة بسرنجة سعة 01 ملمتر .
ال

Live attenuated vaccine of Strept. Equi was prepared by culturing the bacterium
in increasing concentration of acriflavin and the vaccine was given by
installation in the nose of rabbits. The treated group showed immune response,
the titer of the antibodies was 59.4 ± 4.57 after 2 weeks of vaccination, 237±
18.28 after 4 weeks, 475.4 ± 36.57 after 6 weeks, & reach atiter of 512 ± 0 after
8 weeks, and the treated group did not show any signs after challenge dose,
while the control group died after challenge.

Hematological values for sixty clinically normal Arabian bred race horses of both sexes were estimated.Red cell ,count, hemoglobin concentration, packed cell volume, mean corpuscular volume, mean ‘corpuscular hemoglobin concentration, white cell count, differential leukocytic count, total serum protein, albumin and globulin were determined by using several laboratory techniques. It appeared that the total serum protein and globulin were significantly higher in the stallions than those in mares (P < 0.05) whereas the results of other hemaltological parameters were comparable and the mean values did not show significant differences (P > 0.05).

     The aim of the study is to compare the histological changes that occur in the sensory neurons of dorsal root ganglion at L6 and L7 levels of the spinal nerve in different age groups in rabbits. Fifteen rabbits were divided into three groups of equal number according to their age (weaning, maturation and adult). Dorsal root ganglion of spinal nerve at L6 and L7 levels were removed and examined histologically under light microscope. Comparison were made in diameters of neurons and their numbers in different age. The results showed a significant (P<0.05) decrease in the number of sensory neurons and a significant (P<0.05) increase in their diameters with advancing age. In conclusion, the structures of sensory

هدفت التجربة دراسة مدى تأثر قياسات قيم صفتين إنتاجيتين في أغنام العواسي المحلية وهما معدل الزيادة الوزنية (كغم) الأسبوعية وطول خصلة الصوف (سم) المنتج لأجل اعتماد قيم قياسات تلك الصفتين في أمكانية تحديد (درجة المقاومة الذاتية ودرجة الاستعداد) للإصابة بالخمج التجريبي المشترك بمثقبيات الكبد والديدان السلكية لغرض انتخاب وتربية السلالة المحلية (الأكثر مقاومة ذاتية واقل استعدادا للإصابة) كأحد طرق السيطرة على ال

The present study is conducted to in restigate the bacteria Pseudomonas aeruginosa, the impact of ultraviolet on the bacterial isolates under study and the resistance of these isolates to ultraviolet are studied in comparison to two standard isolates ( E . coli and Staphylococcus aureus ) which are considered sensitive to ultraviolet . The natures of the resistance of the isolates, under study, are also being investigated against the different antibiotics. The isolates are subjected to a test to examine their sensitivity to (12) types of antibiotics used routinely in the treatment of various infection of these bacteria. They are (streptomycin , cephalothin ,Gentamycin , cefotaxime ,nitrofurantion ,ampicillin, amoxicillin, rifampin, linco

Eighteen (18) anestrous bitches aged 3-6 years were used in this study. They were diagnosed clinically by external and vaginal examination and the bitches were divided randomly into three equal groups (6 bitches). The 1st group were treated I/M with 20 IU eCG / bitch for 6 days with single injection and 150 IU of hCG hormone /Bitch on day 7 . The 2nd group were treated I/M with 30 IU eCG / bitch for 6 days with single injection and 150 IU of hCG hormone /bitch on day 7, and the 3rd group were treated I/M with 40 IU eCG / bitch for 6 days with single injection and 150 IU of hCG hormone /bitch on day 7. Results showed that the number responded (show proestrus bleeding) were 83.3%, 100% and 83, 3% for the 1st,2nd and 3rd group respectively.

The experiment was carried out at animal farm, College of Veterinary Medicine, Baghdad University from 1/July /2009 up to 28/February2010.Twenty Awssi male Lambs were equally divided into four groups and each animal fed on concentrate diets 2% of body weight with grazing for 3-5 hours a day. The first group(C) was kept as control group , the second group (N) was daily fed with same amount of concentrate diet contain 7.5% ground Nigella sativa seeds , third group (F) also fed with same amount contain 7.5% ground fenugreek seeds ,while the fourth group(NF) group fed the 2% concentrate diet supplemented with both (7.5% of Nigella sativa and 7.5% fenugreek seeds respectively ) as group feeding .
The results revealed that using Nigella sat

Analysis of the decayed apples (van. Starking delicious), collected from the local
market, for patulin mycotoxin, using column chromotography and TLC Plates showed
that the presence of the amount of patulin mycotoxin in the mouldy tissues (1.3 , 2.5 ,
3.4 , 4.2, 5.5, and 6.4 ) cm in diameters were (5.1, 5.8, 6.3, 10.5, 12.3, and 14 ) μg
patulin/g moldy tissues respectively. According to this research also, Patulin
mycotoxin could diffused to the surrounding mouldy tissues. It is found that (0.5and
0.2), (1.0 and 0.5), and (1.5 and 0.5) μg patulin/g tissues in the first and second
centimeters after mouldy tissues 4.2, 5.5 and 6.4 cm respectively, this phenomenon
due to the presence of intracellular spaces in the

This experiment was carried out to determine structural functional changes which could be caused due to uses of dexamethasone at daily therapeutic doses in rabbits for a reasonable period

In order to investigate the presence of methicillin or multidrug resistant Staphylococcus aureus
in food-chain especially Cows raw milk and white raw soft cheese and its whey, a total of 30 samples
were collected randomly from different markets in Baghdad Province during December 2012 till
February 2013, in which samples were analyzed by a standard isolation protocols of food
microbiology with some modification processing by new, modern and rapid technology tools such as
chromogenic medium Baird-Parker agar, Electronic RapIDTM Staph Plus Code Compendium Panel
System (ERIC®) Dryspot Staphytect Plus and Penicillin Binding Protein (PBP2') Plus assays; as well
as, studying the susceptibility of isolates to differe

The study was conducted to investigate the effect of Fenugreek and Ginger Powder. The additives
were added to diets of broiler chicken for 6 weeks (along the period of experiments). The Production
performance, the Physiology and the Microbial balance in the intestine were studied.
150 day-old Hubbard Chicks were divided randomly into 5 groups were as follow:
1- Group one: Basal diet (control group).
2- Group Two: Basal diet to 0.5 % Fenugreek.
3- Group Three: Basal diet to 1 % Fenugreek.
4- Group Four: Basal diet to 0.5 % Ginger.
5- Group Five: Basal diet to 1 % Ginger.
The results showed:
1- The groups which treated by Fenugreek seeds powder (0.5 %) and by Ginger (0.5 %)
and (1%) showed insignificant inc

The aim of study was to investigate the prevalence of macroscopic and microscopic

Hydatid cysts infection rate was 13.56% in buffaloes, slaughtered at Al-
Diwania abbatoir, Al-Qaddisia province,Iraq, during the time period from July,
2000 to June, 2001.The only infected animals were the females, and the higher rate
of infection was found in those aged buffaloes. Lungs,livers, livers and lungs were
infected with 50%, 37.5% and 12.5%, respectively. The total rate of hydatid cysts
fertility was 53.85%, and the sterile, caseated and calcified cysts were 23.08%, 11,
54% and 11.5%, respectively. The mean total rate of viability of protoscoleces was
73.91%, and that of livers and lungs protoscoleces were 82.62% and 70.60%.
Results denoted that the numbers of protoscoleces was increased proportionally<

The aim of this study was to induction of estrous synchronization and
supper ovulation by using intravaginal sponges and injection of different time
and dose of eCG and count the number of corpora lutea and nonovulated
vesicles by using 5MHz ultrasonography in local Awassi ewes
This study were included forty Awassi ewes aged about (3-5) years and 5
rams of same breeds. These ewes allotted randomly into five groups (8 ewes for
each group). Ewes of the first four groups were treated with progesterone
hormone by using intra vaginal sponges. Saturated with 40 mg of flourogeston
Acetate (FGA) for 14 days, while the fifth group was used as a control not
treated with any hormone. Ewes of group 1 and 2 were injected wi

In this study, a sero-survillance on ovine and caprine serum samples from all over Iraq
except the governorates of Iraqi Kurdistan region was conducted. It aimed for the detection of
specific antibodies directed against VP7 protein - the sero-group specific antigen of blue
tongue virus – by using a competitive ELISA technique.The results showed that among a total
of 3277 serum samples, 1436 samples were found to be positive forming 43.82% of total
samples, while 1687 samples were negative with 51.48 % and 154 sample were suspicious
forming 4.69 %. The highest positive serum samples were found in Al Basrah Province
(southern of Iraq) with 60.41% and in Al Anbar Province (Western of Iraq) with 61%, while
the lowes

Seventy four (74) samples from cattle, buffalo, goat and sheep were tested to isolate
the FMD virus, 23 isolates of the virus showed Cytopathic Effect (CPE) on the different types
of cell culture.
The CPE appears after 24 hours on fetal lamb kidney (FLK) cells, 48 hours on the fetal
bovine kidney (FBK), 30 hours on primary Fetal Bovine Thyroid cell (BTY) and 20 hours on
the primary fetal ovine thyroid cells (OTY). Solid phase ELISA test showed three serotypes
of FMD virus O, A and Asia1. One step real time RT-PCR for the diagnosis of FMDV by
using 5UTR FMDV primers and taqman probe (SA-IR-219-246F, SA-IR-315-293R and
SAmulti2-P-IR-292-269R). Samples were tested, showed 23 positive machines by using the
Applied

Salmonella typhimurium and salmonella enteritidis were isolated from infected goat and
prepared an antigens of whole cell sonicated antigen of S.typhimurium
(WCS.Ag.S.typhimurium ),whole cell sonicated antigen of S.enteritidis (WCS.Ag.S.
entertidis) and combination of whole cell sonicated antigen (Salmonella typhimurium and
Salmonella enteritidis) (CWS.Ag) . Their efficacy was evaluated by using tube agglutination
test and enzyme linked immune sorbent assay (ELISA). Twenty rabbits were randomly
divided into four groups; the 1st group was immunized by WCS. Ag - Salmonella enteritidis,
2nd group immunized by (WCS Ags .typhimurium), 3rd group immunized by CWCS.Ag
compound and 4th left as control group which injected

        هدف الدراسة هو نتاج مركز بروتيني محور من الألياف العضلية لأسماك الجري Silurus triostegus ودراسة تركيبه الكيميائي وخواصه الوظيفية والحسية عند خزنه لمدة 35 يوماً في درجة حرارة المختبر 25-30 °م، واستعماله في الانظمة الغذائية إذ كانت النسبة المئوية للحاصل 18.6%، أما المحتوى الكيميائي للمركزالمحور المحضر فقد لوحظ أنّه احتوى على نسبة عالية من البروتين ومنخفضة من الرطوبة والرماد والدهن  ولطيلة

The primary aim of this study was to analyze the nucleocapsid (N) gene of the Canine Distemper Virus (CDV) in infected canines within Baghdad, Iraq. A total of 50 samples, encompassing nasal swabs and fecal specimens, were obtained from canines of various ages and breeds. Utilizing a Rapid Test, nine of 50 samples tested positive for CDV. Subsequent to this, the RNA from the positively tested samples was extracted and subjected to Reverse ‎Transcription-‎Polymerase Chain Reaction (RT-PCR) for cDNA synthesis. The synthesized ‎cDNA was further amplified through conventional PCR targeting the N gene. Six of these amplified products were submitted to Macrogen, South Korea, for sequencing. Sequence analysis revealed multiple silent and

The effect of various concentration of Rosmarinus officinalis on the total count of bacteria in minced meat stored at temperature between 4 – 6oC for periods up to 14 days is examined. The results showed that the oily extract at the concentration of 20 – 100 mg/ml and the watery extract of the concentration of 120 – 200 mg/gm have a significant effect (P<0.05) in comparison with blank treatment No significant variation ware found at (P<0.05) in the treatment with the watery and oily extracts