The aim  of this   study  was to investigate the degree of contamination of  locally produced soft cheese samples by Escherichia coli O157:H7 and to determine the ideal emulsifying salts  and their  appropriate ratios for emulsification of soft cheese and their impacts on microbial load of that influence public health. Samples collected randomly from five popular parties markets affiliated to the city of Baquba 60 samples of the local soft cheese produced by farmers at a rate of 30 samples for each of the summer season, from the beginning of July to the end of August, while the other thirty sample of winter season were collected from the beginning of December to the end of Ja

     The aim of this study was to evaluate virulence of local isolated avian infectious laryngotracheitis virus in experimentally infected chicken. Forty chickens 10 weeks old were used for the experimental infection with the locally isolated infectious laryngotracheitis virus. Chickens were divided into three groups, the first group consisted from 20 chickens infected with isolated infectious laryngotracheitis virus (2×10^4.16 TCID 50/50 µl) via eyes and mouth drops (one drop for each). The second group consisted of 10 chickens (non-infected) in contact with infected group inoculated with maintenance media (Minimum essential medium) on their eyes, to observe if the infected group can spread the virus. The

     This study aim to determine Babesia bovis infection in cattle based on genetic methods. A total of 96 blood samples were collected from alive and slaughtered cattle from different areas in addition to the abattoir of Al-Qadisiyah province from December 2013 to August 2014. Real time polymerase chain reaction (RT.PCR) technique was used to detect the presence of the protozoan with the effect of animal's age and sex in the infection rate 47.91 % (46/96) of examined cattle were given positive result to B. bovis infection. The highest infections were shown among the adult cattle (≥1 year), while there was non-significant difference (P>0.05) in the infection rate according to the sex. So the mos

     In order to identify microsporidia and other fungi  in  stool and  urine samples of human, and in fecal and milk samples of cattle, 100 stool samples with or without diarrhea and 50 urine samples, human fecal and urine samples were obtained from certain Baghdad hospitals and certain rural areas surroundings Baghdad city, in addition to 50 fecal and 56  milk samples  of cattle apparently healthy were collected  from Alshula Slaughter House and  directly from  anal of the animal field  of College of Veterinary Medicine/ Baghdad University. All samples were collected during six months from 1/10/2014 to 1/4/2015. Thin films were formed and stained by Webers Modified Trichr

     This study was designed to investigate the ameliorative role of sodium selenite and melatonin on   acryl amide induced metabolic syndrome in adult male rats. Twenty (20) adult male rats were randomly and equally divided into 4 groups (G1, G2, G3 and G4) and were treated orally for seven weeks G1, (control group) was given distilled water, G2 were given orally 1mg/kg /B.W of Acryl amide, G3 group received 1mg/kg /B.W of Acryl amide followed by 0.5 mg/Kg/ B.W. of sodium selenite orally. G4 group received 1 mg/kg /B.W of acryl amide (orally) followed by 8 mg/Kg/ B.W. melatonin (IP/ injection). Fasting blood samples were collected by cardiac puncture at (0, 7 weeks) of the experiment for measuring serum tot

     This study is conducted at investigating the effect of potassium nitrate and vitamin C in feed of the rabbits on the some biochemical parameters. Twenty eight adult New-Zealand rabbits were divided randomly into four groups (7 rabbits each), they were fed potassium nitrate and vitamin C for 16 weeks as follow: Group 1 (G₁) fed potassium nitrate (168 mg/ kg B.W./ daily), group 2 (G₂) fed potassium nitrate (168 mg/ kg B.W./ daily) and vitamin C (50 mg/ kg B.W./ daily), group 3 (G₃) fed potassium nitrate (168 mg/ kg B.W./ daily)  and vitamin C (100 mg/ kg B.W./ daily) and group 4 (G₄) fed basal ration as control group. Blood were collected from heart at zero, eight, t

     This study was carried out in the laboratories of fish for 90 days in 16 glass aquaria (60 cm, 40 cm and 30 cm/70 L) to investigate the effect of some organic acids salts on some growth indices of common carp fish Cyprinus carpio L. A 128 fish were used with an average weight of (40.8±1) gm. and have been distributed on eight treatments with two replicate and eight fish for each replicate. Formulated eight diets that equally iso-nitrogenous (33.85%) and iso-caloric (1535.605 KJ/gm) were investigated. Organic acids salts (Sodium lactate, Sodium acetate and Sodiumformate) were supplemented singly (0.5% of each salts) and mixed to the experimental diets (0.25% of each salts). The statistical results showed

     This study is conducted on the samples of urine obtained from buffaloes. Eighty six urine samples were obtained from local buffaloes of different ages, of both sex in Mosul abattoir. The results showed that urine of infected animals was cloudy, dark yellow to reddish color with putrid odor of low specific gravity. Microscopic examination of urine sediment demonstrated increased number of pus cell, erythrocytes, epithelial cell, presence of casts and crystals/ high power filed. Chemical examination revealed elevated urine pH and protein content. Serum examination displayed increase levels of urea and creatinine compered to normal urine samples. Culture of urine samples confirmed 24 positive sample

     The present study investigated the effect of synthetic non-methylated oligonucleotides containing Cytosine-phosphate-guanosine dinucleotides (Cytosine-phosphate-guanosine oligodeoxynucleotide) on caprine mastitis with Heat Killed Cryptococcus neoformans Ag. 20 healthy local breed does were used with weight ranging of 25-30 Kg and free of mastitis by examination via California Mastitis Test and Somatic Cell Count. The does were allotted into four equal groups, the first group (G1) was treated intramammary with 100μg/kg of Cytosine-phosphate-guanosine oligodeoxy nucleotide on fifth day postpartum in the right mammary gland while the left mammary gland served as control and were infused wi

     The study aimed to investigate the morphological and histological variations of the aortic arch of local breed rabbits (Oryctylagus conniculus). Nine adult local rabbits were obtained to study the aortic arch (four animals for corrosion cast technique, two injected with colored latex and three for histological study). All the rabbits were euthanized and exsanguinatd, for the cast of the aortic arch they injected by self polymerizing resin via the left ventricle of the heart and the specimens were macerated in (KOH %25) for two weeks and the cast of the aortic arch were prepared. Two main branches were identified from aortic arch (except in one cadaver has three branches). The brachiocephalic tru

     The aim of the present study is to establish the relationship between gestational age and placentome measurement via real-time ultrasound. This study is conducted in the college of Veterinary Medicine at Diyala University. Seventeen multiparous does were synchronized for estrus and naturally served to obtain pregnancy and subsequently scanned using both transducers. Ultrasonic (U.S) examination was performed transabdominally (TA) with sector-probe (5MHz) and transrectally (TR) with linear-probe (7.5MHz), in 10 day interval done started from day 35-135 post mating. The placentome was observed for the first time on day 35 by using TR ultrasonic examination (7.5MHz) linear transducer, as echogenic d

     This study is designed to diagnosis blood Toxoplasmosis in human (women and men) and sheep (male and female) by using Elisa test; as well histopathological examination of heart, liver and lung     of sheep were also done, internal organs were also obtained heart, liver and lung. Women diagnosis was performed by collection 96 samples from aborted women ageing from 15-45 years and divided into three groups; first group 15-25 years involved 50 sample, second group 25-35 years include 37 samples; and the third group 35-45 years included 9 samples. The results of Elisa test showed six positive of IgG, (two for each group) and one positive IgM for the second group, a significant (P<0.05) differen

     The aim of present work is to induce ovulation in 40 female goats in non-breeding seasons and pregnancy diagnosis using RIA of progesterone serum level and trans abdominal ultrasonography with 3.5 MHz prop. The 40 Iraqi goats were naturally inseminated during estrous phase, using fertile backs, after withdrawal of intravaginal impregnated sponges with 20 mg of cronolone               (Fluorogestone Acetate progestagen) kept for eleven days and 400 IU of equine chorionic gonadotrophin inject I/M 24hrs. before sponge withdraw. The results indicated that all does were showed (100%) estrous sign, the estrous time was 24-66 hrs. after sponge withdra

     Some enzymes of seminal plasma such as, Glutamic oxaloacetic transaminase, Glutamic Pyruvic transaminase, Acid phosphatase and Alkaline phosphatase are essential for metabolic processes which provide energy for viability, motility and fertility of spermatozoa. These enzymes are used as good indicators of semen quality as they measure the plasma membrane stability of spermatozoa, so the aims of study are to estimate the enzymes level and total protein in seminal plasma of Holstein bulls. In present the available knowledge there is no study in Iraq regarding the effect of using magnetically treated water on enzymes and total protein in seminal plasma of Holstein bulls born in Iraq to improve the Artificial Insemina

     Acetaminophen also called paracetamol is commonly used as analgesic and antipyretic agent which in high doses causes liver and kidney damage in man and animals. Nigella sativa oil have antioxidant properties. Thirty adult male rats were used and randomly divided into three equal groups. Group (A) untreated and served as control group; Group (B) rats were orally intubated (by gavages needle) acetaminophen suspension (150mg/kg B.W). Group (C) rats were given orally acetaminophen suspension (150mg/kg) plus 1ml/kg B.W of Nigella sativa oil for 42 days in both treated group. Fasting blood samples were collected at 21 and 42 days of experiment to study the following parameters:  Serum creatinine

     The character and timing of gyral and sulci development is one manifestation of the complex orchestration of human brain development. This work describes the morphometry and thickness measurement of human neonate cerebral cortex at age of 28 days. Four Brains samples were fixed in 10% neutral buffer formalin for 24 hrs. Slides from various brain regions were prepared and routine hamatoxylin and eosine staining procedure was applied using histological technique. The present results clarify that the brain mean measurement from the frontal to the occipital pole was 189.6 mm, while the measurements of the frontal, parietal, temporal and occipital cortices were 4.06, 3.84, 3.48 and 3.68 mm, respectively.

     Isolates of Enterohaemorrhagic E.coli O157:H7 were isolated from 51 and 41 of locally produced bovine and ovine soft cheese samples. Their identification were confirmed based on the biochemical reactions and both the morphological cultural and serological properties. Presumptive E.coli O157:H7 isolates obtained by using the conventional selective plating on the chromogenic agar were tested further for the presence of both O157 and H7 antigenes using the latex agglutination test antisera. The current microbiological studies revealed that 31 (33.70 %) out of 92 bovine and ovine soft cheese samples were positive for E.coli O157:H7. The highest non significant (P>0.05) prevalence level o

         Escherichia coli O157:H7 were isolated from 90 bovine and ovine locally produced soft cheese samples and their identification were confirmed based on the cultural, biochemical reactions and serological properties, E.coli O157:H7 isolates obtained by plating on the Chromogenic agars. They were further tested serologically for the presence of both O157 and H7 antigenes using the agglutination test kit. The highest non-significant prevalence (P>0.05) level of E.coli O157:H7 was found in the ewe’s soft cheese samples (37.77%) followed by the cow’s soft cheese samples (31.11%). The antimicrobial potency of the Nisin against the sensitive Lactobacillus

     This study is conducted to establish the profile of some serum biochemicals which included creatinine, urea, glucose, total protein and albumin concentrations in 104 clinically normal donkeys (65 males and 39 females) aged 2-4 years. Animals were classified on the basis of age and sex, in Baghdad city/ Iraq. The Results showed that the, mean values ± standard error (SE) of serum creatinine and urea concentrations were as follows: Creatinine 65.55±2.01 µmol/L and urea 6.79±0.20 mmol/L, while serum concentrations of glucose, total protein and albumin were 54.7±3.09 g/dl, 123±14.3 and 3.4±0.08 mmol/L, respectively. Creatinine values showed a significant difference between 4 years and more than 4 years old sub

     The present study is designed to investigate the histological structure of large intestine of two species of Iraqi birds which belong to two different orders which vary in their kinds of food. Adult birds common kestrel (Falco tinnunculus) that belong to carnivorous and white-eared bulbul (Pycnonotus leucotis) which belongs to omnivorous. This study was carried on 10 birds (5 common kestrel and 5 white-eared bulbul) used for histological study of the ceca and rectum in the two species of birds that include the determination of ceca and rectum, measurements of height columnar cells, villus height, and the thickness of wall tunics. Different types of stains were used including Haematoxylin and Eos

     The study was conducted on 150 healthy and 102 diagnosed iron deficient anemic camels to determine serum iron, total iron binding capacity, unbound iron binding capacity, transferrin saturation, copper and cobalt concentrations. The normal (81 males and 69 females) and anemic (48 males and 54 females) groups both aged between 1-15 years in Najaf governorate- Iraq. Blood samples were collected from the jugular veins into plain tubes during the period November 2014 until May 2015, and the separated sera were used for the measurement of studied parameters. Results showed that the ranges and means ± SE in normal and anemic camels were as follows; Serum iron concentration was 7.37-19.48 μmol/L and 12±0.22 μmol/L,

     This study was conducted on 30 birds (15 birds for each type) divided as 10 birds for each part of study. Anatomical part revealed that the small intestine in both birds kestrel (Falco tinniculus) and white eared bulbul (Picnonotic leucotis) formed from 3 segments; duodenum, jejunum and ileum with no clear demarcation line between them. In kestrel the Meckel's diverticulum appeared as small projection to separate between jejunum and ileum. Both ratio of intestinal length to body length and of intestinal weight to body weight was higher in bulbul than those in kestrel.  Histological study showed that the wall of all three parts of small intestine was composed of the same histological layers;

     This study was conducted to detect virulent isolates of avian infectious laryngeotracheitis virus in Iraq by Real Time-Polymerase Chain Reaction with the amplification of glycoprotein G gene which is responsible for virulence of the virus. Seventy samples (larynx and trachea) were collected from different farms in Iraq to investigate presence of avian infectious laryngeotracheitis virus (detection of virulent isolates from other vaccine strains). Five samples out of seventy samples were virulent isolates (positive result) by using Real Time-Polymerase Chain Reaction utilizing flurescein amidite labeled probe specific for detection of isolates that have G gene (by amplification of G gene) for the first time in Ira

     The aim of this study is to describe the morphology of Cysticercus fasciolaris by using light microscopy, and the pathological changes in the liver of rats naturally infected. A total of 50 liver specimens of local rats (Wister rats) were collected for examination. The gross lesions showed the presence of single or multiple cysts. Microscopic findings revealed the presence of larvae within the cysts which represent the larvae Cysticercus fasciolaris of the adult parasite Taeniae taeniaeformis which inhabited the small intestine of the domestic cats surrounded by fibrous connective tissue infiltrated with inflammatory cells (mononuclear cells and plasma cells). These lesions cause pressu

     Common carp fish (Cyprinius carpio) is a native Asian and Eastern Europe, so there are many types of teleost fishes in Iraq. This study was designed to explain the environmental effects on the tissue of gills of fish. Twenty six of carp fish specimens were collected from Tigris River of Taji region in Baghdad city during Winter and Summer in January and August months respectively. Also the samples were collected from rearing aquarium by breeders of the fishes with water temperature recorded (31°C), in summer and (8 °C), in winter season via thermometer through the specimens collection in each time. The seasonal temperature levels were considered important in the histological alterations of the gills, w

     Forty eight (48) anestrus lactating Iraqi ewes aged between 2-4 years were undertaken 40 days postpartum in Babylon province (Technical institute in Al-Mussaib). Ewes were randomly divided into 4 equal groups according to type of treatment implemented. Group 1 (G1) were treated with vaginal sponges impregnated with 60 mg Medroxy Progesterone acetate for 10 days. Immediately after sponge removal, each ewe was injected IM. with 500 IU. Equine choriogonadotropines. Ewes of G2 were treated with Medroxy Progesterone acetate and Equine choriogonadotropines as mentioned above in addition n to 10 mg bromocriptine/day orally for 10 successive days at the same time of sponges insertion. Animals of G3 were treated with Medr

     This study aimed at evaluating the efficacy of different concentrations of C. craniiformis mushroom suspension in pigeon flock infected by Hadjelia truncata worms in Diyala province in comparison with 0.1% ivermectin. Thirty pairs of pigeons were brought to the veterinary clinic with case history of progressive weight loss, weakness, and some birds have died suddenly. In the necropsy of 3 cases the gizzards contained some nematodes, located under the koilin layer of the gizzard, which was identified microscopically as Hadjelia truncata which was one of the important parasites of the digestive system of poultry. Three different concentrations of suspension were prepared from

This study was designed to investigate the effect of molybdate and sulfate overload alone or in combination overload intake on copper outcome in mature male rats. Twenty eight adult male rats were housed and randomly divided into four equal groups and treated as follows for 60 days: control, groups (T1) rats received sodium molybdate 50 mg/kg B.W and (T2) rats of this group were given orally 500 mg/kg B.W of sodium sulfate. Animals in the (T3) group were given sodium molybdate and sodium sulfate in combination at half dose in comparison with T1 and T2 doses. The severity of toxic symptoms were more noticed at the end of experiment especially in T1 followed by T2, while T3 showed the least toxic symptoms, also the body weight change recor

     This study was carried out to investigate the hepatotoxicity of chronic adminstration of an immunosuppressant Tacrolimus in male albino rats. Thirty male albino rats were randomly divided into 3 equal groups, the first one (T1) recieved therapeutic dose (70µg/kg.BW) of Tacrolimus. The second group (T2) recieved double dose (140 µg/kg.BW) of Tacrolimus, while the third group recieved distilled water for the same period and considered as control (C). Alanine aminotransferase, Aspartate aminotransferases, Alkaline Phosphatase, serum potassium and serum calcium showed significant increase in both treated groups T1 and T2 as compared  with control, proportional to the dose of Tacrolimus. There were histopatholo

     The current study was carried out at the college of veterinary medicine, University of Baghdad, for three months. The main objective of this study was to evaluate the effect of different thawing method of frozen minced red meat to reduce its bacterial load. Four thawing methods were used where the tap water at 18 °C for two hours was used in the first method, while the hot water at 40 °C for 1.5 hours was used in the second method and in the third method the frozen minced meat samples were left at room temperature (28 °C) for two hours, where as in the fourth method the frozen meat samples were stored inside the refrigerator at 4 °C for 20 hours. Data revealed that there were  significant differences (P&