Garden cress (Lepidium sativum) seeds are considered extensively in the folk medicine as anantidiabetic agent in many countries. The current investigations focuses attention on theglucose and lipid lowering effect of the aqueous extract of L. sativum seeds on experimentally induced diabetes in rats. The biochemical parameters studied were plasma glucose, insulin, totalcholesterol,triglycerides,phospholipids,hemoglobin, and glycosylated hemoglobin.
In addition body weight and renal glucose reabsorption were notified. Aqueous extract of L. sativum were orally administered daily for 30 days in a dose of 20 mg / kg body weight to alloxan – diabetic rats , and a significant reduction in the parameters measured was investigated compared t
The aim of this study was to identify the main risk factors with Trichomonas vaginalis infection in married women that have vaginal discharge. The study include (250) female who attended Al-Yarmouk Teaching Hospital (Gynecological Clinics), through the period from February 2010 to July 2010.Patients female were subjected for a special questionnaire sheet. The study has found that (65) females were infected with this parasite from total (250), who were complaining from vaginal discharge with infection rate 26%.Higher infection rate was recorded between pregnant female was 17.2% while non-pregnant women 8.8% .The main age group of infected women concentrated in (20-29 years) in which rate were 12.83% and 11.76% in pregnant and non-pregnant
... Show MoreThis study was undertaken with the aims of evaluating the efficiency of the PCR assay in investigation of the epidemiological status of tropical theileriosis in comparison with the classical microscopic examination of Giemsa-stained blood smear and detecting the gross and microscopic pathological changes in some organs of the infected animals. Blood and tissue samples were collected from 50 apparently healthy, tick-infested cattle during the period extended from the 1st of July to the 31st of August, 2008 in the modern slaughterhouse in Sulaimaniyah province. Blood smears were prepared from the blood samples and stained with Giemsa dye. The tissue samples were obtained from normal and lesion-presenting tissues of the lymph nodes, lungs a
... Show MoreThe experiment was carried out at animal farm, College of Veterinary Medicine, Baghdad University from 1/July /2009 up to 28/February2010.Twenty Awssi male Lambs were equally divided into four groups and each animal fed on concentrate diets 2% of body weight with grazing for 3-5 hours a day. The first group(C) was kept as control group , the second group (N) was daily fed with same amount of concentrate diet contain 7.5% ground Nigella sativa seeds , third group (F) also fed with same amount contain 7.5% ground fenugreek seeds ,while the fourth group(NF) group fed the 2% concentrate diet supplemented with both (7.5% of Nigella sativa and 7.5% fenugreek seeds respectively ) as group feeding .
The results revealed that using Nigella sat
The presence of Bacillus species Were investigated in the intestine of 50 fish specimens common carp Cyprinus carpio weigh range between 1400-1500g in weigh were obtained from commercial farms in north of Baghdad through period of September to December 2010.The result revealed presence of gram positive, rod shape bacteria grow in the mesosphilic temperature range. The optimal temperature was 25-35C° facultative aerobes and produce catalase an enzyme. The bacterial antagonist activity was tested with fish culture pathogenic bacteria. The modified antagonistic method results showed that Bacillus subtilus could inhibit Aeromonas hydrophila after 24 hours. The highest level of antibacterial substances of this bacillus was produced in 48 hou
... Show MoreBehavioral problems are very important because they are one of the most common reasons for the relinquishment of cats. In this study, the frequency of behavioral problems in 167 cats attending Small Animal Hospital, Faculty of Veterinary Medicine, University of Tehran, was evaluated with the use of a questionnaire. Interaction with other animals and people, outdoor access, breed, and sex of the cats were evaluated for association with the occurrence of the behavioral problems. Aggression towards people and other cats, Elimination problems, Scratching objects, Fearfulness, Attention seeking, Excessive grooming, Chasing small animals, Hiding, Vocalization in owner's absence, Obsessive behaviors and Eating fiber material were the behavioral
... Show MoreSixty cows were included in this study which was conducted in Baquba for the period from April 2011 to October 2011. They were clinically diagnosed as having bovine ephemeral fever (BEF) infection. Blood samples were collected from infected cows before, after treatment, and after recovery. Additionally, 30 healthy cows were included as control group. Sera were separated and submitted for the determination of phosphorus, glucose, cholesterol and pH levels. Statistical analysis of biochemical values revealed significant decrease in serum cholesterol levels in infected cows as compared to control group (P<0.05). Furthermore, the diseased cows showed low levels of pH (acidosis), thus when they treated by intravenous injection of 5% sodium
... Show MoreThe histological study of the pecten oculi of falcon eyeball is consists from 11-12 pecten plates connected to the pectin bridge. The pectin oculi extending from optic nerve toward the virtous body. Its characterization are highly visualization and continuous capillary network which infiltrated by melanocyte. The pecten oculi without muscles and nerve fibers. The study revealed that cornea of falcon eyeball was characterized by fine curvature, translucent with peripheral thickness. The cornea occupied the quarter of fibrous tunica. It consists from five layers: - anterior corneal epithelium, Bowman's membrane, stroma, Descement's membrane and endothelium.
Forty seven blood samples were collected from local house cats 25 male , 22 female , ranging in age from 1-15 year for both sexes for measuring the following parameters coagulation tests (total Platelets count, Clotting time, Prothrombin Time & Fibrinogen concentration ) and proteins concentration tests (Total protein concentration, Albumin , Globulin concentration ) and iron and copper concentration tests .the results as were follow (228.5 –1,537) X 109\L , (2-5)min , (6 - 15) sec , (0.5 -4) g\L , ( 61 - 83 ) g\L , (23.1 -38.2) g\L , (25.9-54.2) g\L , (5.21 – 45.56) μmol/L , (2.29 - 36.52) μmol/L) .the result showed significant differences at level (P<0.05) between the male and female of local house cats in total Platelets
... Show MoreThe investigation on Heat-Intolerance Syndrome following foot and mouth disease (FMD) infection in cattle in ThiQar–Iraq, used 3ABC FMD ELISA kit, and Radio- immunoassay (RIA) to detect the cortisol level. From 105 there were 65(62%) infected cattle with FMD, which was high at 5- less 8 years old, while the cortrisol level showed three levels; normal (13-21 nmol⁄L), high and low levels were; 6(5.6%), 44(42%) and 55(52%) subsequently. More over the combined result of ELISA and RIA had divided cattle into six groups. First group of 40(38%) cattle infected with FMD and had low level of cortisol, this group containing 21(20%) with clinical signs of heat intolerance. Second group contain 22(21%) FMD infected cattle with high level of cort
... Show MoreFor studying in vivo influence of immune responses on side effects of Mitomycin C (MMC),Sixty-five male-white mice were divided to nine groups ;1st group inoculated MMC,I.P,0.4mg/ml,2 doses weekly/4 weeks;2nd to 7th groups immunized S/C with LM Ags(25mg/ml) protein concentration 0.4 ml, 2doses/2 weeks intervals;2nd and 3rd groups immunized CFLMAgs and CFLMAgs-MMC in 3rd group; also 4th and fifth groups but immunized with WSLMAgs; 6th and 7th groups inoculated with 0.4 ml Attenuated LM (LaLMAgs), 7th group immunized-MMC treated. Post-immunization, 5 animals sacrificed from 1-7groups and negative control to study the cellular and humoral immunity as well as bone marrow taken to cytogenic examination ,other animals from 1-7 groups and 8th g
... Show MoreThis study was conducted on local breed goats to determine the changes in biochemical parameters in anemic cases by using morphological classification, one hundred and seventyserum samples were collected randomly from goats of both sexes aged 3 months – 6 years, they were grazing on pasture from the different districts of Baghdad, Bakuba and Falluja. The total protein, albumin and fibrinogen in the normal bucks were 73 1 – 84 1 g/L, 35 3- 37 1 g/ L, 9 8- 11 1 g/ L, respectively and in the normal does were 66 1 – 77 4 g/ L, 35 6 – 37 2 g/ L and 6 – 15 g/ L, respectively, the average of these parameters differed significantly at (p> 0.05) between the normal and the anemic animals. The mean level of copper in the serum of norma
... Show MoreIn this investigate Cholera toxin was purified from the local clinical isolate
designated as V. cholerae S . Few steps were employed for purification of CT
including concentration of the protein, back extraction, and gel filtration. The
bioactivity of CT reflected that crude and purified toxin had lethal activity on Balb/c
mice in which a dose of 2.5µg/ ml of purified toxin caused mice death with
symptoms such as muscle cramps and tachycardia while erythematous and
induration was seen at intradermal I. d injection of skin of Guinea pig.
Histopathological examination for liver, intestine, adrenal gland and spleen of white
mice showed that liver tissue was more affected compared with spleen, intestine and
adrena
أجريت هذه الدراسة للكشف عن تأثير إعطاء حامض الفوليك بجرع مختلفة ) 5 مايكروغرام و 01 مايكروغرام( يومي ا
لكل حيوان على بعض المعايير الدمية في ذكوور الأرابوب البالغوةم توس ت سويس الايوابوائ عشوواىيا إلوى ثيثوة مجوامي متسواوية
مجموعة سيطرة ومجموعتي معالجة ، عوملت المجموعة الأولى T1 باامض الفوليك بجرعة ) 5 مايكروغرام( ومجموعة
المعالجة الثابية بجرعة ) 01 مايكروغرام( واستمرئ التجربة لمدة أربعة أسابي لغرض دراسة بع
صممت هذه الدراسة لمعرفة التأثير الضار لفرط المثيونيي للوا الجزوال التلاسولذ الوذكري للجورااض فبالألوافة الوا
معرفوة التوأثير النيوالذ للمسوتكلو الللونلذ للسبيوب الأسوند . وا يسوتكدام ثماييوة لذور مو اكونر الجورااض البالغوة ويسومت
لذنالياً الا ثلاثة مجاميع متساوية ولنملت كالا ذ لمدة 42 ينم 1 مجمنلوة السويطره - C (جرلوت مللونا الودار ؛ 2 -
مجمنلة المعاملة الأولا GI ( جرلت الجرااض فذ هوذه المجمنلوة 100 ملغوا
أجريت هذه الدراسة في نينوى على الجاموس المحلي، إذ جمعت البيانات عن 154 جاموسنة فني ليلني مخيل ني فني
المحافظة لدراسة إنياج الحليب اليومي وذلك للمندة من 1/7/2010 ولغاينة 18/7/2010 ولخمسنة أينام ميوالينة يالينوم ا و
والخامس والياسع والثالث عشر واليوم السنابع عشنر منع جسنجير درجنات الحنرارة الونغرى والعظمنى. جنم جحلينر البياننات
باسنيعما يريينة ا نمنوذج الخاني العنام ي General linear model-GLM ضنم البرننام SAS لدراسنة جناتير
تهدف الدراسة الحالية التحري عن تأثير المستخلص المائي لثمار نباا الطبا Tribulus terrestis فاي بعا
المعايير الكيموحياتية وهي الفوسفاتيز الطاعادي والحدياد والفوسافا والداوديوم و البوتاسايوم واسلدوساتيرول و الكورتيكاو
ستيرود في ثلاثين أرنب ا مان الاوكور البالغاة قسام حيواناا التررباة علا ساتة مرااميا المرموعاة اسولا هاي مرموعاة
السيبرة ، و المرموعة الثانية جرع ب 051 ملغم\كغم\يوم من المستخلص المائي للطبا ، والمر
هدفت الدراسة الحالية لتحديد تاثير نبات الحنظل فى بعض الصفات الفسلجية كوزن الجسم ، بعض معايير الدم
ووظيفية الغدة الدرقية لذكور الارانب انجزت تجربتين لستين ذكر ارنب بالغ و قسمت بشكل متساوي وعشوائي الى 03
ارنب في كل تجربة . تحوي كل منها على ثلاث مجاميع معالجة وثلاث مجاميع سيطرة وكل مجموعة ضمت 5 ارانب .
في التجربة الاولى والثانية وبشكل منفصل , اعدت الارانب المعالجة) (T1, T2 and T3 groups وجرعت في التجربة
الاولى بجرعة
تضمنت الدراسة الحالية التغيرات الشهرية في قياسات الخصى والبرابخ ونشاط الذكور في الجاموس العراقي.
تضمنت الدراسة 052 خصية أخذت من مجزرة شرق مدينة كربلاء وللفترة من شهر كانون الأول ولغاية شهر ايلول
,أجري فحص عياني للذكر قبل الذبح للتأكد من سلامته الصحية. وأجري تقييم للسائل المنوي ) الحركة الفردية ،الحيوية
،التركيز والتشوهات (للسائل المسحوب من ذيل البربخ .وزنت الخصية اليمنى بعد الذبح وأخذت أبعاد الخصية والب
شملت الدراسة الحالية 89 مبيضا ، جمعت من مجزره الشعلة مباشرة بعد ذبح الحيوان ،حفضت المبايض في المحلول
ألفسلجي بدرجة حرارة 73 م ونقلت إلى المختبر خلال 4 7 ساعات ، تم قياس قطر الجريبات المتواجدة على المبايض -
وقسمت إلى جريبات كبيرة الحجم ) 01 6 ( مليمتر وصفيره الحجم ) 5 0 (مليمتر . سحبت البويضات المحاطة بالركمه - -
المبيضة بطريقه الشفط من الجريبات الكبيرة والصفيرة بواسطة ابره قياس 09 كيج موصولة بسرنجة سعة 01 ملمتر .
ال
Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs, causing
acute hemorrhagic enteritis and myocarditis. In this study different mutant strains of the virus
were characterized by polymerase chain reaction (PCR).The fecal samples from infected dogs
suspected for CPV2 infection were collected in a suitable medium. The viral DNA from fecal
samples was extracted using specific kits, PCR were carried out with five different primer,
pCPV-2ab and pCPV-2b, to distinguish the strain prevalent in field condition. The primer
pCPV-2ab recognized both variant CPV-2a and CPV-2b, whereas the primer pCPV-2b
recognized only the variant CPV-2b, using the third primer pCPV to recognize the residual
base pair
In this study, a sero-survillance on ovine and caprine serum samples from all over Iraq
except the governorates of Iraqi Kurdistan region was conducted. It aimed for the detection of
specific antibodies directed against VP7 protein - the sero-group specific antigen of blue
tongue virus – by using a competitive ELISA technique.The results showed that among a total
of 3277 serum samples, 1436 samples were found to be positive forming 43.82% of total
samples, while 1687 samples were negative with 51.48 % and 154 sample were suspicious
forming 4.69 %. The highest positive serum samples were found in Al Basrah Province
(southern of Iraq) with 60.41% and in Al Anbar Province (Western of Iraq) with 61%, while
the lowes
Salmonella typhimurium and salmonella enteritidis were isolated from infected goat and
prepared an antigens of whole cell sonicated antigen of S.typhimurium
(WCS.Ag.S.typhimurium ),whole cell sonicated antigen of S.enteritidis (WCS.Ag.S.
entertidis) and combination of whole cell sonicated antigen (Salmonella typhimurium and
Salmonella enteritidis) (CWS.Ag) . Their efficacy was evaluated by using tube agglutination
test and enzyme linked immune sorbent assay (ELISA). Twenty rabbits were randomly
divided into four groups; the 1st group was immunized by WCS. Ag - Salmonella enteritidis,
2nd group immunized by (WCS Ags .typhimurium), 3rd group immunized by CWCS.Ag
compound and 4th left as control group which injected
There was a noticeable increase in cancers, congenital malformations, spontaneous
abortion and other cases among Iraqi population after both Gulf Wars I and II, the present
study aimed to the detection and determination of internal radiation pollution with Depleted
Uranium (DU), and investigate the effects of this type of radiation exposure on some
immunological parameters for the first time in Iraq. A total of 88 blood samples were
collected from 45 patients and 43 healthy persons during 2009-2010; from the outpatients of
the Institute and Hospital of Radiotherapy and Nuclear Medicine and Al-Kadhymia Teaching
Hospital in Baghdad. Results: 1. Contaminated persons with DU were 48 persons (54.5%)
from total 88 perso
In this study (35) cows suffering from bovine papilloma were subjected for three different
types of treatments; The First group involved 15 animals treated with autogenous vaccine.
The Second group involved 10 animals treated with prepared cell culture vaccine .While the
third group involved 10 cows treated with virulent local Newcastle disease virus. In the First
two groups the warts were surgically removed from cows showing lump lesions on skin of
abdomen, neck and udder, and transferred aseptically to laboratory by using transport media.
Treatment of cows in the first group involved preparation of autogenous formalin (0.5%)
in activated vaccine. Vaccination of these animals result in regression of the warts starte
Seventy four (74) samples from cattle, buffalo, goat and sheep were tested to isolate
the FMD virus, 23 isolates of the virus showed Cytopathic Effect (CPE) on the different types
of cell culture.
The CPE appears after 24 hours on fetal lamb kidney (FLK) cells, 48 hours on the fetal
bovine kidney (FBK), 30 hours on primary Fetal Bovine Thyroid cell (BTY) and 20 hours on
the primary fetal ovine thyroid cells (OTY). Solid phase ELISA test showed three serotypes
of FMD virus O, A and Asia1. One step real time RT-PCR for the diagnosis of FMDV by
using 5UTR FMDV primers and taqman probe (SA-IR-219-246F, SA-IR-315-293R and
SAmulti2-P-IR-292-269R). Samples were tested, showed 23 positive machines by using the
Applied
One hundred sheep with bilateral otitis externa were studied one year and a half .The
exudates of both external ears obtained using sterile swabs, and microorganisms were isolated
according to standard microbiological techniques. There was bacterial and fungal growth in
many of the samples. The total numbers of isolates from right ear was 104 , and the most
common pathogens isolated in right ear were Staphylococcus aureus 30.76 % , then
Staphylococcus epidermidis 9.61% and Pseudomonas aeruginosa 9.61 % while E.coli ,
Proteus mirabilis and Streptococcus pyogenes were 7.69% for each of them , Candida
albicans 5.76% , Klebsiella pneumonia 3.84% ,Nocardia spp 3.84% , Pasteurella multucida
3.84% ,Mannheimia haemolytica
In order to determine Candida Species isolates that associated with urogenital infection in women,174 vaginal swabs and 66 urine samples were collected from pregnant and non-pregnant women suffering from urogenital tract infection whom visted teaching Al-way hospital during the peroid from January to October 2011. The results showed that 32 out of 174 vaginal swabs were Candida Spp positive (18.3%), high percentage was reported in June and July (25%) for each one and no fungal isolates were recorded in March .The results also explained that high percentage of fungal isolates was recorded in pregnant women (68.7%) as comparing with non-pregnant women (31.2%) .Thirty –two fungal isolates were recorded which included : C.albicans(43.7%);
... Show MoreThis study was conducted to extract and titrate the leukotoxin of Fusobacterium necrophorum isolates recovered from 75abscesses found in 24 livers of slaughtered cattle in Sulaimaniyah region. The culture supernatants of these isolates were subjected to the tetrazolium dye reduction test which revealed that the leukotoxin titer values of 34 F. necrophorum subsp. necrophorum isolates ranged from 128 to 1024 (with a leukotoxin titer mean of 516±46), whereas the leukotoxin titer values of the 11 F. necrophorum subsp. funduliforme isolates ranged from 0 to 128 (with a leukotoxin titer mean of 73±12).
This study is considered the first study in our country to isolate bovine parainfluenza
type 3 virus (BPIV-3) from naturally infected calves. BPIV-3 virus was isolated from nasal
swabs collected from calves with respiratory disease. The virus was isolated and titrated in
secondary embryonic bovine kidney (SEBK) cell culture; other cell cultures (secondary sheep
testis and chicken embryos fibroblast cell cultures) were used for propagation of isolated
virus. Examination of infected cell culture revealed giant cell formation and intracytoplasmic
inclusions. The isolated virus agglutinated guinea pig’s erythrocytes and produces clear
heamadsorption of guinea pig’s erythrocyte in infected SEBK cell culture. Virus tit