A total of 250 stool specimens from patients live in Al-Ameen, Al-Mashtal, Al-Baladiyat and Al-Nahrawan, districts of Baghdad city were collected. The watery and loose specimens were wet mounted with normal saline and buffered methylene blue then all specimens concentrated and examined with Lugol's iodine solution. The protozoal parasites of medical importance Blastocystis hominis and Giardia lamblia were respectively detected in 59 (24.6%) and 42 (17.5%), samples with mixed infection were in eight samples. Differences due to district lacked significance. Statistical significance was observed when the influence of age category was tested (P<0.05). The highest infestation percentage with both parasites was noticed in the 5 – 10 years
... Show MoreAs a part of a protocol ( Z2F10 – Laidlomycin calf - fed Holstein health management ) Coccidiosis was studied in two hundred Holstein steer- calves (114 kg ) to evaluate the effect of management and treatment programs. Early weaned animals were shipped to the University of California Desert Research Center El Centro on the 26th of May 2010. The calves were vaccinated and treated with internal and external anti parasitic also injected with Vital E - AD (5ml SC 100000 vitamin A/ml and 12ml of liquamycin. Steers were balanced by weight and assigned within weight grouping to (40) pens ( 5 steers / pen ). Animals were randomly divided into four equal groups, the 1st was control group and the 2nd, 3rd and 4th groups were treated with Monensi
... Show MoreRabies is a highly fatal infection of the central nervous system which occur in all warm-blooded animals and transmitted by the bites of affected animals. The major manifestations of the disease include motor irritation and clinical signs of mania and an attack complex and ascending paralysis. Rabies and rabies related viruses belong to a group of more than 75 viruses called RHABDOVIRUSES (Rhabdo=rod) because of their bullet-shaped
... Show MoreFor the determination of FMD infection in Nainawa province Serum
samples were collected from 92 suspected cases (42sheep,50cattle)this
was performed in September 2007 and included nine region(Talafar
,Basheca, Al-shamal, Hamamalaleel ,Cokajlly ,Zamaar ,Al-Namrood
,AL-Koosh and Hemedaat), these samples were send frozen to the labs of
state Vet. Company in Al-Nahdaa , the special test FMD-3ABC bo Ov
enzyme immune assay on sheep serums showed that 30.95% were
positive to the test and 19.05% were ambiguous while the test on bovine
serums showed increase in both positive and ambiguous results
(42%,26% respectively), 8 serums that showed the highest positive result
to the former test were submitted to the special
The aim of this study to investigation the effect of Anaplasma spp.
infection on some blood parameters by collecting 65 blood samples
from Awassi local breed sheep. The total infection rate 26.15 % which
divided into 25 % in males and 26.31 % in females. The parasitemia
range between 0.5-4 % (mean 1.97 %).
The infected animals showed anemia represented by decrease means
of packed cell volume (P<0.01), red blood cell count (P<0.05) and
haemoglobin concentration, While increase corpuscular volume,
corpuscular haemoglobin and corpuscular haemoglobin concentration
(P<0.05). As well as decrease total white blood cell count and monocytes
while increase lymphocytes, neutrophils , eosinophils, basophils and
To conduct the influence of salinomycin & anticoccidial vaccine on
pathological changes in intestine of broiler chickens experimental
infected with Eimeria spp. By using 40 broiler chicks divided randomly
to four groups( 10 chicks of each group ) First group vaccinated with
anticoccidial vaccine (coccivac) at 8 days of age in drinking water while
the second group feeded salinomycin in concentration 60ppm from one
day old ,the third group left non vaccinated nor given salinomycin as a
control group at(26 days )of age chicks in that three groups infected with
different species of Eimeria spp .with high dose (744x74³) Oocyst /chick
by group inoculation & the fourth group consider negative control not
v
Sera from1722 cattle of different age (3 months to 8 years) were
examined by the Rose Bengal and tube agglutination test: 134 cow give
positive result and the overal Brucella seroprevalence was (8%) milk
samples zeropositive milking cattle were cultured on Brucella selecative
media.
Seropositive cattle (102) were treated for the first time with long acting
oxytetracyclin at the dose of 20 mg/kg of body weight administered
intramuscularly (i.m) every 2 days for 30 days and streptomycin at 25
mg/kg (i.m) every 2 days for 16 days.
The regimen was found to be effective in eliminating the shedding of
Brucella organisms by cattle in milk.
Moreover all treated cattle became zeronegative within 16 months after
This study was designed to determined the influence of levamisole
administration as immunostimulator for sheep vaccinated with Brucella
melitensis vaccine (Rev-1). The sixty sheep (males and females) were
divided equally into four groups *The first group (vaccinated group ) the
animals were vaccinated with Rev-1 vaccine at a dose of 1 ml s/c which
contain 2 x 109 viable bacteria *The second group ( vaccine and
levamisole ), was vaccinated with Rev-1 as the same of the first group as
well as levamisole was given orally at a dose of 10 mg / kg B.W a week
before vaccination and with vaccination and a week after vaccination and
then levamisole was given at monthly intervals up to the end of the
experiment.* Thir
The present work making histological studies of certain part of medulla
oblongata on seven one humped camel ( Camelus Dromedarius ) of different
ages and sexes. The location of Vagus X, accessory XI and hypoglossal XII
nerve nuclei. The hypoglossal nerve XII nucleus consist of two nuclei dorsal
greater and ventral smaller and the dorsal nuclei observed. Connected with
dorsal motor vagal nucleus X by specific arch of fiber in many sections and
another observation the root fiber of hypoglossal nucleus XII pass along the
lateral border of inferior olivary nucleus and some sections the root fiber
penetrate lateral part of inferior olivary nucleus.
This study was designed to investigate the prophylactic effect of aqueous
extract of black currant concentrate on some biological markers related with
heart disease in male rat treated H2O2. It also aimed at comparing the
prophylactic effect of black currant concentrate to that of vit E.
Forty adult male rats were divided randomly into equal groups (ten
rat/group) & were treated as follows for 42 days. Rats in the first group (G І)
were received normal water with oral intubation of sun flower oil 1ml /rat and
consider as control group. Animals of the second group (G Π) were received
0.5% H2O2 in drinking water, while rats of the third group (G Ш) were received
0.5% H2O2 in drinking water with oral intuba
The main aim of this study is to detect infection of Wild brown rats with
Mycobacteria . fourtyone Wild brown rats were collected from various
quarters of Baghdad city during the period 29/5/2004 – 30/10/2004 .
fourty one rats collected from college of Veterinary Medicine farm
Baghdad University and from farm of Abu – Ghraib , Baghdad –AL
Gedida , AL – Mahmoedia , AL – hurrea . In this study after collection
of rats the post mortem change was studied and take one hundred twenty
three sample of Lung , Liver and Spleen for bacterial examination one
hundred twenty three samples were culture on special media for
Mycobacteria . The result revealed that four rats were infected with
Mycobacteria . four is
Escherichia coli is consedered to be one of the normal flora of the human
and animal intestinal tract, but there are some serotypes which are diagnosed
as a pathogenic such as Enteroheamorrhagic E.coli O157 that cause disease
ranging in severity from mild to lethal and it's significant as a major foodborne
pathogen and as a public health problem. The purpose of this study
was to provide the prevalence of E.coli and E.coli O157 in locally produced
soft cheeses, detection and enumeration were carried out using specific
differential media (CT.SMAC,E.M.B.), serological test (Latex O157) and
biochemical test.
A total of 50 samples of locally produced soft cheeses were collected from
supermarket in Baghdad City du
The present study performed to evaluate the impact of addition of
Thymbra spicata leaves powder of aflatoxin contaminated ration in
reduction of the aflatoxin immuno suppressive effect in the immune
response induced by Newcastle disease vaccine in broilers.
The study conducted in three groups, The first group fed aflatoxin free
ration, second group fed aflatoxin contaminated ration without any
treatment, while the third group fed aflatoxin contaminated ration
supplemented of 2% Thymbra spicata leaves powder.
All chick groups were vaccinated twice with Newcastle disease vaccine
(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate the
humeral immune response at 6, 19 and 31 days of age. Infec
Teaser rams were used for discovering estrus in ewes because this technique
is very important for super ovulation,embryo transfer and transgenic animals
programs.sever techniques were used for preparation of teaser animals
especially in sheeps so this study was designed to compare between two
surgical methods (vasectomy and epididymectomy).
Six Assaf of breed rams,Clinical reproduction system,libido and laboratory
examination (volume ejaculate,mass and individual motility and sperm
concentration). were performed 2 week before experiment for qualification
of the general health of the animals the animals were divided into two equal
groups, Vasectomy in the first group and epididymectomy in the
another.Cases we
The study was aimed to comparison of anthraxin prepared by a modified
method from cell-wall extract of avirulent B .anthracis strain 34F2(Sterne)
with antigens extracted from the same strain as 1/40 antigen, autoclaved 1/40
and the crude toxin. These antigens were compared on their induction of cellmediated
immunity (CMI) in guinea pigs. Animals were immunized and
boosted subcutaneously with the Sterne live veterinary anthrax vaccine. Two
weeks after the booster dose, animals were skin-tested with the four antigens.
Anthraxin was the most active antigen which recorded 16.66 mm a mean of
erythema and 2.3 mm a difference of skin thickness after 24 hours. Both 1/40
and autoclaved 1/40 antigens gave approximately t
This study was carried out at the poultry farm of the college of
veterinary medicine / Baghdad University during 12/5/2004 – 6/7/2004 to
study the effect of substitution of smut wheat instead of intact wheat in
poultry ration at the following levels (0%, 25% , 50% and 100%)
representing the following groups (T0, T1, T2 and T3), respectively. Physical
treatment was obtained for smut wheat by polishing the seed by sand to
remove the superficial fungal growth. Each group was sub divided into two
sub groups, one was physically treated with sand and the other was not
treated to study the hematology traits at 28 and 56 days of the experiment.
Characterristics included in this study were PCV, Hb, RBC, WBC,
differ
This study was carried out to evaluate the ability of Lactobacillus
acidophilus to prevent and treat Salmonella typhimurium in puppies. In vitro
antibiotic sensitivity test of S. typhimurium was made and the result revealed
that Ciprofloxacin is the most effective.Isolation of L. acidophilus from the
intestinal tract of the normal dogs and also revealed that all Lactobacillus strains
were biochemically identical to standard strain. A bacterial strain that had high
ability to inhibit the growth of S. typhimurium in vitro with high ability to
adhere to intestinal epithelial cells and tolerate the low pH and bile salts was
chosen for the experimental study.
Twenty puppies were divided into four groups and treated
In present experiment , study was done on the pathological and residual effect of malathion in mice 50 white mice randomly divided into 3 group : 1st group including (20)mice was given single oral dose .1.5 ppm in drinking water 2nd group contain (20) mice given single dose 1.5 ppm malathion orally in drinking water and repeated the same dose at 2nd and 3rd week i.e (three repeated doses), the 3rd group (10) mice act as control group .The long of experiment was 60 days , the following parameter were done .
1- Clinical Signs: treated group 1st and 2nd groups showed nausea and diarrhea
2- Pathological changes : 1st and 2nd gro
... Show MoreA total of 143 samples of meat were examined in this study, these
represents 67 samples of locally minced meat, 31 samples of imported
minced meat and 45 samples of imported chicken meat. The samples were
collected from different areas of Baghdad city during the period from
January till May 2006.
Detection, isolation an enumeration of E.coli 0157:H7 were carried out.
The result revealed that there was a significant difference (P < 0.05) in the
isolation percentage and the microbial counts of E.coli 0157:H7 when
comparing the three types of meat. The highest prevalence were found in the
locally minced meat (80% ; 1.6 x 107 cfu/g) Followed by the imported
minced meat (65% ;5.9x106cfu/g) and finally chicken
An outbreak of avian influenza (AI) (H9N2) occurred in broiler, layers and
breeder chicken farms in Iraq during 2004-2007. Mortality between 30% and
70% in broilers and 5% and 10% in layers and breeders were commonly
observed on the affected farms. Mixed infections of the influenza virus with
other respiratory pathogens particularly Newcastle Disease (ND) virus and
Infectious Bronchitis (IB) virus were thought to be responsible for such high
mortality. ELISA test was used to detect ND, AI and IB antibodies. Clinical
signs included , decrease feed consumption, swelling of the face and typical
severe respiratory signs. Gross lesions included extensive hyperemia of the
respiratory system, cheesy exudates in the tr
This study was carried out to at the poultry farm of the college of
veterinary medicine / Baghdad University during 12/5/2004 – 6/7/2004 to
study the effect of substitution of smut wheat instead of intact wheat in
poultry ration at the following levels (0%, 25% , 50% and 100%)
representing the following groups (T0, T1, T2 and T3), respectively. Physical
treatment was obtained for smut wheat by polishing the seed by sand to
remove the superficial fungal growth. Each group was sub divided into two
sub groups, one was physically treated with sand and the other was not
treated to study the hematology traits at 28 and 56 days of the experiment.
Characterristics included in this study were PCV, Hb, RBC, WBC,
dif
This study aimed to prepare a synbiotic of Sacch. cerevisiae and used as 0.1 and 0.2 % in feed (T2,T3) for reducing the experimental infection of Salmonella typhimurium to broiler chicks ( -6103days) by using 40 birds divided into four treatments , T1 was negative control with 0.1 % synbiotics and T4 was positive control with experimental infection only , production
and blood parameters were studied , and the results showed the following: A synbiotic of Sacch. cerevisiae was made which have 10 7 cfu of
Sacch. cerevisiae and 10 mg Mannan Oligosaccharide per gram .Using 0.1 and 0.2 % of Sacch. cerevisiae synbiotic reduced experimental infection of Salmonella typhim
... Show MoreThe main objective of this study was to determine the effect of copper
deficiency on some milk constituents of ewes.(Thirty)raw milk samples have
been collected in the morning and before feeding of the ewes,(10) of them
from ewes suffering from copper deficiency and the other(20) milk samples
were collected from healthy ewes .The percentages of fat ,protein ,lactose,
chloride, specific gravity, moisture and the pH of milk were determined.
The results have shown that copper deficiency have an effect on the milk
constituents where all milk samples collected from ewes suffering from
copper deficiency had significantly (p<0.01) lower percentages of fat
(5.70%) ,protein(3.0%), lactose (3.4%)
and specific gravi
Twenty five Isolates of Salmonella were isolated from raw milk
samples which were collected from Abu-Graib village to study the
relationship between their resistance to antibiotics and their plasmids
contents .
Antibiotic sensitivity test for different antibiotics revealed that most
Salmonella isolates were resistant to two antibiotics .
The extraction of the DNA-plasmid revealed that most Salmonella
isolates which resistant to two antibiotics or more were contained large,
individual plasmids size of (55-75)Kilo Base (KB) while the others
isolates were free from such plasmid
This research included study the effect of garlic and onion plants
extracts(alcoholic and watery) in vitro in three different concentrations
15%,25%,35% and in vivo in experimental white mice .Research was
performed by three experiments, first one was conducted to study
effectiveness of different concentration of alcoholic and watery garlic
extract on growth of candida albicans and Cryptococcus neoformans in
vitro, showed that the effect of alcoholic extract on the growth of candida
albicans was inhibitory,started from 0.4 mm to 0.1 mm compared with
control plats 4.2 mm ,where as the results of the effect on the growth of
Cryptococcus neoformans showed more clearness and the inhibition
started from 0.6 to in
The main objective of this study was to isolate, identify and
enumerate the E.coli O157:H7 bacteria and coliform bacteria from
locally manufactured soft cheese.
A total of 50samples of local soft cheese were collected and examined
from local markets of (5) different areas of Baghdad city which was { Aldora,
Al-Amria,Al-Kadmia,Al-Karada,Al-Kazalia} 10 samples for each
area.
The results showed that the highest average counts of E.coli O157:H7
and coliform bacteria was in Al-Dora 73.63 × 105and 956 × 105cfu/ml
while the lowest average counts in Al-Kazalia 3.12× 105and 43.7×
105cfu/ml .
The result of the statistical analysis showed that there were a significant
differences (p<0.05) between E.coli O1
This study was concerned on the production of Bacillus anthracis
toxin and its use as a vaccine. Two groups of guinea pigs were
immunized with the toxin which was given in a dose of 0.5 ml
subcutaneously to the 1st group while the animals of the 2nd group were
immunized by instillation in the nose 4 drops into each naris. The two
groups were given booster dose 14 days after the 1st dose. The immunized
and control animals were bled by cardiac puncture 20 days after the
booster dose.
In the present study, anthraxin was used as an antigen in Enzyme
Linked Immuno Sorbant Assay (ELISA) to measure the optical density
(OD) obtained from adminsteration of vaccine in two different routes.
High mean of optical dens
تضمن البحث دراسة شعاعية لمعرفة تأثير الزرع الذاتي (Autogenous Graft) لنخاع العظم على سرعة غلق الصفيحة المشاشية القاصية لعظم الفخذ المثقبة تجريبيا باستعمال تقنية فمستر المحورة للصفيحة المشاشية في 6 كلاب من السلالة المحلية قسمت عشوائيا الى مجموعتين ضمت كل مجموعة 3 كلاب الاولى اجريت عليها عملية تثقيب الصفيحة المشاشية باستخدام تقنية فمستر المحورة و كانت هي مجموعة السيطرة اما المجموعة الثانية (
... Show MoreA three species of clam Unio tigridis, Pseudontopsis euphraticus and
Anodonta sp. were collected from Al-Habaniya lake, Al-Angor region/Iraq
.The animals were exposed to three concentrations of mercury :0.1, 0.2 and
0.4 mg/L . First group of animals were exposed for three weeks ,second
group for two weeks and the third group for one week. At the end of the
exposure period, the animals were dissected ,then the mantle removed for the
further studies on proteins and enzymes. The results were :
1- Total protein concentration in the mantle decreased in the three
species of clam exposed to mercury. It was observed that a
prominent effect of mercury on the electrophoretic bands of
proteins with the decrease in the
The study included 82 clinical mastitic cows (328 quarters) in two dairy
cattle stations, 15 cows in the first station in Baghdad and 67 cows in the
second station in Babel, Clinical inspection was conducted on cows and it’s
udder, milk samples were collected under sterile condition and subjected for
physical, chemical & bacteriological examination with antibiotic sensitivity
test of the isolated pathogens.
The mastitic cows in each station were divided into two groups, the 1st
(Treated group) in which cows treated by us and the 2nd (control group) in
which cows were treated by the staff of station. After treatment all cows
were examined clinically and bacteriologly four times (once 10 days). Swaps
fro