This study was carried out to detect the Cryptosporidium spp in Karbala province, Iraq from. December 2019 to September 2020. Age, sex, and months interference with parasite prevalence were studied. A total of 100 fecal samples were collected from adults and young and from both sexes of human. Fecal samples were subjected to conventional methods (Flotation Methods by Sheather's sugar solution and stained with modified Ziehl-Neelsen) for parasite diagnosis. The result recorded that the infection rate of Cryptosporidium spp was 26%. The age group of 2-6 years had the highest infection rate comparing to other age groups ranged from 12-25 years yet was marginal significant (P<0.06). Regarding sex, there was no

The adrenal glands are endocrine glands that produce a diversity of hormones comprising of adrenaline, the aldosterone and cortisol. The present study aimed at investigation of the histomorphometric features of chromaffin cells. There were two types of chromaffin cells. In squirrel, the first type was columnar in shape and brownish in color contained spherical nucleus located at the base of cells, which represented the epinephrine secreting cells, and the second type was polygonal in shape and light brownish in color contained spherical nucleus located in the center of cells, which represented the norepinephrine secreting cells. The adrenal medulla of hamster consists almost entirely of columnar or polyhedral

Conventionally reared goats and experimentally inoculated goats with Mycoplasma agalactiae were examined for the presence of

An indirect ELISA technique was used to determine the prevalence of infection in sera and milk samples among goats and sheepin Duhok, Kurdistan Region, Iraq. Results of the present study shows that the prevalence of infection was higher in sera compared to milk samples among goats and sheep as follow: (6.3, 4.2%) and (8.9, 5.6%) respectively and there was no statistical significance difference in goats at P value =0.42, also there was no statistically differences at P value= 0.22. ELISA results also shows that the prevalence according to the age among goats and sheep were higher in animal aged > 2 years in both sera and milk samples were (7.5, 5.3%) and (11.3, 7%) respectively and there was no significant difference between age groups

     The aim of this study was to determine the level of antibiotics residues in the locally produced cows raw milk as well as to evaluate the effect of different commercial heat treated process on the level of antibiotics residues in milk. A total of 52 locally produced milk samples were collected from apparently healthy cows in AL-Fudhailia village, milk cans (50 Kg), bulk milk tanks (1, 5 and 10 tons) which belonged to the milk reception and collection centers and dairy plants in Baghdad were collected during beginning of December 2015 to the end of April 2016. Results revealed by using high performance liquid chromatography that there was a significant seasonal variation in the residual concentration of each detec

     This study included isolates of bacteria from 125 clinical samples in Erbil and Kirkuk Hospital including (burns, wounds, urine and sputum); 38 isolates were identified as P. aeruginosa after conducting microscopic and biochemical tests. The results of antibiotic sensitivity test showed that all isolates of P. aeruginosa  were different in resistance to Pipracillin, Erythromycin with rate of (100%) and to the Nalidixic acid (94.73%) while the lowest resistant antibiotics were to Co-trimoxazole, Ceftazidime and Ciprofloxacin, which amounted to (26.31%, 23.68 and 21.05%) respectively. For molecular diagnosis of P. aeruginosa some virulence genes the alg D and exo A

         The aims of present study were designed to determine the relationship between fetal number single or twin and placentome measurement by real time Ultrasonography at different stage of pregnancy. Twenty mature female of shami goats were synchronized  for estrus with vaginal sponges impregnated with 60 mg of medroxey progesterone acetate (MAP) (Esponja-vet – spanin) under strict  hygienic conditions, the sponges were inserted by applicator for 14 days, following  by eCG (Folligon) intra muscularly injection each female goats (doe) received  500 IU Folligon. At the time of sponges withdrawl, estrus detection was done and each doe expressed estrus sings serviced naturally

     Four hundred fecal samples from diarrheic calves of both sexes (1-60 days old) from four Iraqi Mid-Euphrates governorates (Babylon, Al-Najaf, Karbala and Al-Qadissiyiah) were collected to investigate rotavirus type A infection by using Real-Time Polymerase Chain Reaction technique. The results showed that infection rates were (14%), while the high rate (20%) was found in age of (1-30 days), however non-significant values were related to animal gender. Moreover, a high infection was recorded at January (29.5%) and the rotraviral infection was recorded in four provinces but the higher was in Babylon (23%). The clinical examination of infected calves showed voluminous watery diarrhea, profound weakness and mild depr

     This study aimed to evaluate-the inhibitory effects of ethanolic and-chloroformic-extracts of local medicinal plants as Oak (Quercus-acuta), Thyme (Thymus vulgaris) and Cinnamon (Cinnamomum-zeylanicum) prepared in different concentrations (50, 100, 200 and 400 mg/ml) against the growth of Listeria-monocytogenes isolated from infected humans and animals and comparing their activity with effectiveness of the standard antibiotics. The results showed detection of Listeria-monocytogenes in 3 of (50) blood samples collected from aborted woman making 6% samples, while the detection rates of Listeria-monocytogenes in milk samples collected from sheep and cattle were 4

     This study was carried out to investigate the cytogenetic effect of Levofloxacin after In-ovo inoculation. Forty eight fertilized eggs were used, divided equally into eight groups and inoculated through shell puncture above air cell with materials in equestion  and incubated for two different periods three and seven days. They were assigned as group one and group two 10 µg Levofloxacin, group three and group four 20µg levofloxacin, group five and group six considered positive  control 0.05 µg Mitomycin-C, group seven and group eight (Phosphate Buffer Saline) considered negative control groups. All eggs from in-ovo experiments were subjected to cytogenetic tests, such as Mitotic Inde

     The study aimed to investigate the anatomical and histomorphometrical features of spleen in gazelle. To achieve this goal, spleens of 7 animals were used. The gross aspect of the study revealed that the spleen of gazelle appeared dark brown in color with elliptical shape and was situated at the left lateral surface of the rumen. It occupied the area extended from the 8^th rib to 11^th rib. Length and width of the spleens were calculated and the mean were 7.94±0.1, 5.88±0.2 respectively. There were three ligaments connected the spleen with adjacent structures, namely splenogastric, splenorenal and splenophrenic. The blood supply of the spleen was studied using X-rays and resin injection metho

     The study was conducted to indicate the contamination of the local produced broilers carcasses by Escherichia coli O157:H7, and its effects on public health. Sixty samples of local produced Broilers were collected randomly from five different areas in Baquba center of Diyala governorate, (Iraq). Thirty samples to each were collected in summer, from the beginning of July to end of August, and in winter season from the beginning of December to end of January to investigate their microbial load. All summer samples had significantly (P<0.05) higher microbial count of Coli-form Bacteria, Escherichia coli and E.coli O157:H7 than winter samples, but there were no significant differ

     The aim of the present study was to specify the relationship of gestational period with measurement of Crown-Rump length and Bi-parietal diameter via trans-abdominal ultrasonography in goats. This study was conducted in the Faculty of Veterinary Medicine in Diyala University. The study was carried out on twelve multiparous does, their ages were between 2-5 years. Does were synchronized for estrus with PGF₂α (Estrumate) at 11 days apart and naturally served to obtain pregnancy (estrus day=day 0), and subsequently Ultrasonic scanned. Weekly ultrasonographic examinations were performed using 3.5-5MHz sector transducer, from week (5-10) and (6-14) of pregnancy for measuring the Crown-Rump length and Bi-pa

The present study aimed to synthesize selenium nanoparticles (SeNPs) using aqueous extract of black currant as a reducing agent. The green synthesized black currant selenium nanoparticles (BCSeNPs) were identified by color change. The characterization of SeNPs was achieved by Ultraviolet-visible (UV–VIS) spectroscopy, scanning electron microscopy (SEM), X–ray diffraction analysis (XRD), and Fourier transform infrared spectroscopy (FTIR). These tests were used to detect: stability, morphology, size, crystalline nature, and functional groups present on the surface of BCSeNPs. The results revealed appearance of the brick-red color indicating the specific color of selenium nanoparticles, and UV-Vis spectroscopy showed band absorbance at

Calotropis procera is announced to be medicinal and poisonous plant to human and animals. In this study, the toxicological effects of aqueous leaves extract were evaluated experimentally in rabbits. The median lethal dose (LD50) was estimated at 2435.25 mg/kg BW using the Up and Down method. Twenty-five local breed rabbits, 1-2-year-old and 1-1.9 kg BW, were divided randomly into five groups with five rabbits each. Those of groupsI, II, III and IV were exposed to 1/10 of LD50 (243.5mg/kg), 1/12.5 of LD50 (194.8 mg/kg), 1/15 of LD50 (162.4 mg/kg), and 1/20 of LD50 (121.8 mg/kg BW of extract respectively for 8 weeks, while those in G.V were left without exposed as a control group. Alkaloids, saponins, tannins,

The present study was conducted to evaluate the effect of scaffold fabricated from Nano crab shell and Al-kharit (Papyrus Vaccine) for enhancing the healing of the experimentally induced bone defect in dogs. For this purpose, twenty healthy adult mongrel dogs were used in this study which divided randomly into two equal groups, under general anesthesia, 1 cm bone gap was created in the distal part of the tibia, that fixed by bone plate and screws. Nano crab shell scaffold was implanted. All experimental animals showed normal situation without any infection at the site of operation, while the radiography showed a periosteal and endosteal reaction. Moreover, the gaps were bridged faster in the treated group as compared with the control gro

Sixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq for molecular evaluation. The extracted nucleic acid was amplified using reverse transcriptase polymerase chain reaction (RT-PCR) targeting genes of segment A (Vp2, Vp3, Vp4 and Vp5 genes) and segment B (VP1 genes). The products of amplification were sent to Korea for sequencing using Sanger method. The sequencing analysis of the IBDV from the Iraqi isolates revealed that each gene had different transition and transversion (nonsense and missense of point mutation) compared to reference genes. The phylogenetic tree analysis showed that the VP2

This study was planned to isolated and identify some psychrotrophic aerobic sporeforming bacteria from

اللقاحات الحية تختلف اللقاحات الحية ضد مرض کمبورو في ضراوتها وتركيبها المستضدي وطرق استعمالها

Powder of pomegranate barks (Punica granatum L) was prepared. Different concentration of watery

 Comparative studies about the characteristics of the acute toxicity of streptomycin and gentamicin and

Mosquitoes are vectors of various life-threatening diseases like malaria, yellow fever, dengue fever etc. Their close proximity to human habitations allows ease for disease transmission. They have been identified by key morphological tools like their wings, legs, bristles etc. but closely related species are difficult to identify based on morphology. Molecular tools have, therefore, been employed to help with the more accurate identification. This study was aimed at identifying and characterizing different mosquito species in five different states in North-Central Nigeria using internal transcribed spacer 2 (ITS2) and mitochondrial 16S rDNA regions. Mosquito larvae were collected from stagnant water in breeding places at each collection

The study was performed to investigate the protective effect of different methanolic and oily extracts of leave and dry date of Phoenix dactylifera against oxidative stress induced by CCl4 on 49 Sprague-Dawley male rats weighed 175-200 g and aged 6-8 months. The animals were equally divided into 7 groups and assigned as follows: G1, administered 0.1 mL distilled water orally and considered control negative group (C-ve); G2, administered 0.1 mL/100 g BW corn oil (CrO); and G3 administered 100 mg/kg BW CCl4 orally for induction oxidative stress and considered control positive group (C+ve). The other four groups were initially administered 100 mg/kg BW CCl4 for oxidative stress induction and treated for two mont

This study was aimed to understand the hematological changes in the level of Hb and Met-Hb

This study was designed for isolation and molecular identification of Nontuberculous Mycobacterium (NTM) from fish during the period between October and December 2017 from Karbla province, Iraq. This study included 200 fresh fish samples from four different species including Spondyliosoma cantharus, Liza abu, Carassius carassius and Cyprinuscarpio. Three samples of each fish were taken including gills, muscles and all internal organs. The samples were processed by decontamination, concentration of 4% sodium hydroxide, and 0.1 ml of sediment was streaking on Löwenstein Johnson (LJ) media; then the bacterial cultures were incubated at 28-30 °C for 3days up to 4 weeks and suspected colonies were stained with a

This research was carried out to evaluate the effect of poly d, l-lactic-coglycolic acid(PLGA) nanoparticles loaded with peptide (as vaccine) on histomorphological in duodenum of broiler chick. A total of ninety eight, one day old ,unsexed broiler( Rose) chicks were divided randomly into seven groups( 2 replicate in each group) as follows:G1-control,G2 - chicks received traditional vaccine of infectious bursal disease(Volvac@ IBD MLV) ,G3 – Chicks Received PLGA nanoparticles only, G4,G5,G6 and G7 – chicks were received prepared vaccine at 160, 80, 40,and 20 μg of peptide loaded PLGA respectively. At the end of the experiment histopathological examination of duodenum section and histomorphological changes

The present study aimed to investigate the morphological features of the pancreas and duodenum of the adult males and females guinea pigs. Eight animals of each sex were collected to conduct this project. The selected organs were photographed in situ and macro morphometric measurements were conducted on them. Gross findings revealed that the pancreas of guinea pig was of compact type, of two lobes (right and left) connected by large central part (body). The organ drains the pancreatic secretion toward the last part of the ascending duodenum via minor pancreatic duct with absence of major pancreatic duct. The duodenum of the guinea pig was very short and V-shaped. The beginning of the duodenum contains duodena

This study aimed to examine the cytotoxic effect of resveratrol as an anticancer in human colorectal cancer (HRT) cell line by assessment of its half-maximal inhibitory concentration (IC50) and its ability to inhibit the growth of these cancerous cells. Resveratrol inhibited the proliferation of HRT cell lines when used at different increased concentrations in this study (25, 50, 100, 200, and 300 μmol). These increased concentrations of resveratrol caused a corresponding significant inhibition in the growth percentage of the tested cancerous cell line (13%, 31.33%, 53.66%, 63.66 %, and 76.33%, respectively) when compared with DMSO0.1% as negative control, in a concentration-dependent manner. Resveratrol at

This study was conducted to investigate the prevalence of avian malaria (Plasmodium gallinaceum) in the local domesticated breed chickens (Gallus gallus domesticus) that were purchased from the local markets in Baghdad city, using 100 blood samples which were collected from the wing vein, and kept in EDTA-K2 tubes for conventional PCR analysis during the period extended from 1 /10 / 2018 till 31/ 3 / 2019. Total infection rate was 18% (18/100), which were divided into males 20.00% and in females 16.00%. The eight isolates were recorded in the GenBank under accession numbers ID: MN082405.1, MN082406.1, MN082407.1, MN082408.1, MN082409.1, MN082410.1, MN082411.1, and MN082412.1 with identity 99.20 - 99.87% and w

The present study was conducted on 10 Iraqi pregnant buffaloes aged between 3-6 years at Al-Qasim district- Babylon province, Iraq from December 2018 to December 2019. Those animals diagnosed by rectal palpation and ultrasonography technique to estimate the reproduction status. The results of this study reported wandering of ovum in 10% of animals (1/10). While ultrasonography characterized by increasing placentomes measurement gradually with development of gestation, and the placentomes recorded 8.32±2.46 mm, 13.27±3.25 mm, 18.37±5.34 mm, 23.25±8.63 mm and 28.2±6.87 mm in the 2nd , 3rd, 4th, 5th and 6th month, respectively. Also the total size was recorded by the crown rump length (CRL), which was 0.77