Outbreaks of rinderpest in cattle occurred in Mosul area between February to June 1987. The morbidity and mortality
... Show MoreOne hundred and fifty stool sampling were collected from clinical sources responsible of causing diarrhea in adults as well as children. The identification of Aeromonas hydrophila isolates depended on common methods of identification dependent on biochemical characteristics and culture, then vitek2 compact system was used. Eight Aeromonas hydrophila isolates were gained and revealed various productivity of metalloprotease; the isolate number 8 was the maximum effective in metalloprotease production. The eight isolates were examined with Polymerase Chain Reaction to prove enzyme gene presence, the results revealed that all isolates were positive for ahMP genes; metalloprotease was comple
... Show MoreSix deeply anestrus Arabian mares associated with small, hard inactive ovaries were treated with synthetic
... Show MoreFrom Ministry of Health records from 1989-1995, Giardiasis in human being was recorded betw
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Perfluorooctanoic acid (PFOA) is a synthetic fluor-surfactant chemical used widely in products that resist oil, heat, grease, stains, and water. It is also used in producing other fluoropolymers. The main sources of exposure to PFOA are water, soil, and animal-origin food (meat, fish, and dairy products). The aim of this study to evaluate the renal function following oral gavage of sub-lethal dose of PFOA in diabetic and non-diabetic guinea pigs. The experiment run for 4 weeks, total of 40 male guinea pigs, (Cavia porcellus), were randomly selected and grouped into four equal groups. The first group (G1) served as the negative control; 2nd group (G2) alloxan induced diabetic, 3rd |
The aim of the current study was to compare the efficacy of Kessler suture pattern and polypropylene meshes implantation to repair severed Achilles tendons in bucks. For this purpos 16 local adult bucks, weighing 30-35kg were used and equally distributed into two groups, the first group: (control group) and the second group (mesh group). Animals were sedated with xylazine 2% and anesthetized locally with lidocaine hydrochloride 2% infiltrated subcutaneously. Skin incision approximately 5 cm. in length was made over the Achilles tendon. The tendon was isolated by blunt dissection from the underlying tissue, then the left Achilles tendon was transected in its mid portion. In the control group, tenorrhaphy of Achill
... Show MoreThe study achieved isolating Campylobacter spp from diarrheic and non-diarrheic cows and studying the percentage of diarrhea in 10 areas in Baghdad governorate (AL Shaeluh, Abou Ghurayb , Khadmyia , Sadar city , Suwyria, Mahmudyia, Latifyia , Alridwaniuh, Hur Rajb and Yousfyia), One thousand fecal samples were cultured on different selective culture media specific for Campylobacter spp ,and different biochemical tests were used (oxidase reaction, Oxoid biochemical identification system campy, Tripple Sugar Iron ( TSI) , hippurate hydrolysis and the use of Vietik (NH). Eighty isolates of Campylobacter spp were recorded ( 8% ).Camp
... Show MoreIn this investigation the common carp, Cyprinus carpio L. 1758 was exposed to contaminated feed with Aspergillus flavus under laboratory aquarium conditions (glass ponds) for 90 days. Results revealed that the contaminated feed with (A. flavus) has significantly affect in body weight change, blood and biochemical parameters of groups fed on contaminated feed with A. flavus. Body weight changes were decreased in group feeding on contaminated feed with A. flavus in comparison with control group. The results showed decreases in RBCs, PCV% and Hb concentration but WBCs was increased in treatment fish in comparison with the control group. In addition, serum Glutamic pyruvic
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The multipotent characteristic of rabbit adipose-derived stem cells makes them available and convenient sources for isolating mesenchymal stem cells. The aim of this study was to assess the differentiation in rabbit adipose-derived stem cells pre-committed to produce several mesenchymal lineages in response to inductive extracellular cues to multipotent stromal cells. Three grams of adipose tissue was taken from a subcutaneous region of the nape of the neck and was carefully isolated to obtain mesenchymal stem cells for expanded by fourth passage. In the 4th passage, active growth of mesenchymal stem cells was observed. Furthermore, the research demonstrated the inherent ability of rabbit MS |
Nerium oleander leaves ethanolic extract at median lethal dose of 520 mg/kg body weight were administered orally to six male mice .Clinical signs of toxicosis in mice began to appear about 1 hr after receiving the oleander extract which included abdominal pain, frequent urination, diarrhea, depression, weakness and convulsive movement and death at the end stage.Four mice died within 4 to 24 hrs and two survived. Haemorrhage spots of varying degrees were observed in internal organs at necropsy.Histopathology revealed extensive tubular necrosis in kidneys with haemosiderin pigment in the cytoplasm of convoluted tubular cells, different degrees of coagulative necrosis in cardiac muscle cells associated with haemorrhage and infiltration of m
... Show MoreSafe and effective anaesthetic regimens have been described for use in rabbits,
partially because of the susceptibility of this species to fatal respiratory arrest. The
present study was conducted to evaluate the efficacy of anesthetics, analgesics
combinations an anesthesia in twenty five local breed rabbits. Rabbits were injected
intramuscularly by: Propionylpromazine 0.5mg/kg B.W. as premedictation, after
10minutes later Xylazine and Ketamine Hydrochloride at a dose of 20mg/kg,
50mg/kg respectively. The results of the physiological parameters of the control
group at the period of zero time concerning rectal body temperature, respiratory rate,
heart rate were 38.00±0.29 ºC; 96.36 ±3.33bpm; and 147.20±6.46/mi
The mixing of ginger Zingiber officinale and garlic Allium sativum used at different concentrations ( ginger 10g/kg; garlic 10g/kg; ginger 5g/kg with garlic 5g/kg; ginger 7.5g/kg and garlic 2.5g/kg and ginger 2. 5g/kg and garlic 7.5g/kg in treated 1, 2, 3, 4 and 5 respectively) as a dietary supplements based on their performance in enhancing the fish healthy while control diet was free from ginger and garlic. Haematological parameters of 96 fishes of common carp Cyprinus carpio L. were determined after two months, Red blood cells count (RBCs), white blood cells count (WBCs), hemoglobin concentration (Hb) , and Packed cell volume (PCV) , total plasma protein , albumin and Globulin, liver enzymes (ALT,AST and ALP) using several laboratory
... Show MoreThis study was carried out at Animal Farm, College of Veterinary Medicine, Baghdad University from April up to July 2013. The animals were fed on concentrate diet and freely grazed for 3-6 hours/ day at College Field, rams were used in a Latin square design (4*4) and trans located biweekly intervals, respectively to different concentrate diets, the first diet 2% of the body weight which was free from any addition and considered as a control, the second diet was contain 3% Cuminum cyminum seeds (CU), while the third diet was contain 3% of Ocimum basilicom seeds (B) and the fourth concentrated diet was contain 1.5% of Cuminum cyminum and 1.5% Ocimum basilicom seeds (CU+B). Results r
... Show MoreIn order to investigate the in vitro antitumor activity of Datura stramonium seeds on cancer cell lines. Extract of this plant was prepared by using different concentrations of seed extract 3, 3.25, 3.5, 3.75, 4, 4.25, 4.5, 4.75 and 5 mg/ ml. Cytotoxicity was estimated on mammary adenocarcinoma (AMN3), brain cancer, and normal rat embryonic fibroblast (Ref3) cell lines. The results exhibited that the extract has cytotoxic effect by decreasing the viability of AMN3 (42.91%) and brain cell lines (32.79%). However, it produced little effect on viability of normal cell line Ref3, indicating the specificity of this extract against malignant cells
One hundred samples of ration, protein, corn, soybean, and wheat were collected from poultry houses in Baghdad and suburbs to examine Aflatoxin occurrence in chicken's diet. ALISA technique was used for this purpose.The results revealed presence of high level of Aflatoxin in ration (P< 0.05) compared with other contents of diet fallowed by soybean (P< 0.05) then corn, wheat, and protein respectively .These contents were mixed and tested by ELISA and presented to the experiment chicks. One hundred and twenty, one day old chicks, were divided to four groups, first group (G1) was fed contaminated Aflatoxin feed and did not vaccinated. The second group (G2) was fed contaminated feed and vaccinated with Newcastle Disease vaccine (NDV) a
... Show MoreThis study was designed to investigate the protective effect of Pomegranate seed oil (PSO) on kidney damage induced by methionine overload in adult female rabbits. Twenty four adult female local rabbits were randomly divided into four equal groups, and they were treated daily for 42 days,as fallow: the first group (C): rabbits were received ordinary corn oil orally, serving as control, while aniamls in the second group T1 were intubated with methionine (100 mg/kg BW) orally and animals in third group (T2) were intubated with methionine (100mg/kg BW) plus pometon (30mg/kg BW) diluted in corn oil wihle, rabbits in
... Show MoreThis study was conducted on 20 adult mice which were divided randomly in to 4 equal groups. The first group served as negative control (NC) that received the diluent (sun flower oil), while hyperlipidemia was induced in the other three groups, the second group received the diluent and considered as positive control (PC), while group three and four were treated with olive oil (OO) or Nigella sativa oil (NSO) at a dose of 0.4ml/kg BW orally, respectively for two months. At the end of experiment and fasting overnight, lipid profile which included (total cholesterol TC, triglyceride TG, high density lipoprotein cholesterol HDL-C, low density lipoprotein cholesterol LDL-C and very low density lipoprotein chol
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Coxofemoral luxation is a common orthopedic lesion in dogs. This study aimed to evaluate the effectiveness of a modified toggle splint with nylon suture as a prosthetic teres ligament for reducing and fixing coxofemoral luxation in dogs. Five adult mongrel dogs weighing 15±2 kg were used in this experimental study. The toggle splint was fabricated from a Steinmann pin (1cm length, 2mm diameter) by drilling 0.5 mm hole in the center. Stabilization of coxofemoral joint was performed by a modified Toggle splint technique. A tunnel, from the major trochanter through the fovea capitis to the acetabulum, was performed in which the toggle splint was passed through, and nylon suture was used for |
This study aimed to investigate the in vivo antidiarrheal efficacy of methanolic extract of Portulaca oleracea against diarrhea induced by Escherichia coli in male rats. The initial phase involved the extraction of P. oleracea using 99.8% absolute methanol through a Soxhlet extraction apparatus. Phytochemical analyses of the extract unveiled the presence of alkaloids, flavonoids, steroids, carbohydrates, tannins, and proteins. In the experimental phase, 20 Wistar albino male rats (Rattus norvegicus) were divided into four groups: the Negative Control (uninfected and untreated); the Positive |
A total of 660 genital tract of adult ewes slaughtered at Baghdad Abattoirs, were examined for
the gross pathological conditions .One hundred thirty three (20.22%) of them showed different type
of abnormalities of the total genital examined, 157 (23.87%) were pregnant and the rest 503 were
non-pregnant ones. Eighteen (11.46%) of the pregnant and 115 (22.86%) of the no pregnant genitalia
showed pathological conditions, Ovarobursal adhesions 51(7.72%) and paraovarian cysts 35(5.31%)
were the most common abnormalities recorded. In conclusion: Such kind of abnormalities observe in
genitalia slaughtered ewe could be considered as the causes of temporary infertility or permanent
sterility, never the less, the percent
This study was conducted to know the effect of ewe age on oocyte quality as well as the relations
between oocyte viability and normal uterine condition. Eighty three (83) reproductive systems of
non-pregnant ewes were collected from Al-shulla abattoir. The Total oocytes were aspirated from
right ovaries reached 61.45% and 38.55% from left ovaries. Immediately after aspiration, the oocytes
were examined by light microscopic and conceded as mature if surrounded completely with cumulus
oopherus. While the stained oocytes by trypan blue were conceded as dead oocytes and excluded.
According to ewes age the oocytes were classified into (3) groups, the first group ranged between 1-
2 years, second group 3-6 years and th
The study was conducted to evaluate antitumor effects of green tea (Camellia sinensis) extracts (aqueous and methanolic) on Rhabdomyosarcoma; cell line and a normal cell line; mouse embryo fibroblast; Chemical detections of green tea extracts revealed that the aqueous and methanolic extracts were positive for flavonoids, alkaloids, phenol and glycosides. The percentage growth inhibition of five plant concentrations (50, 100, 250, 500 and 1000 µg/ml) were assessed in vitro using tumor cell lines Rhabdomyosarcoma and normal cell line mouse embryonic fibroblasts. The results revealed that the five concentrations of the plant extracts showed anti-tumor properties in a concentration-dependent manner
... Show MoreThis study was performed to evaluate the reliability and efficacy of intra-peritoneal performances of chitosan powder in preventing abdominal adhesions following laparotomy. Twenty clinically healthy rabbits of both sexes, weighing 1.5-2 kg were allocated randomly into 2 equal groups; control group (G1) and treated group (G2). After surgical preparation the animals had undergone to laparotomy which was performed to create a sero-muscular incision of 4cm length in the stomach, in G1 the incision was closed by suturing stomach wound and abdominal wall, While (G2) animals were subjected to same operation but after stomach incision suturing, 1gr. of chitosan powder was sprinkled on stomach incision and peritoneal cav
... Show MoreThis study focuses on diagnosis of Clostridium difficile causing Antibiotic – Associated diarrhea and colitis by Elisa method and to detect of C. difficile Toxin A and B in stool samples by Elisa test. Two hundred forty (240) stool samples were collected from children suffering from antibiotic- associated diarrhea and Colitis cases at ages from after birth to 15 years old from Baghdad hospitals. Samples were taken during the period of first of June 2013 until the end of April 2014. In addition to that 80 samples from healthy children of the same age and sex as a control group. Clostridium difficile Toxin A and B in stool samples were detected. Results of this study in
... Show MoreThis study conducted to find out the protective role of ethanolic extract of Eruca sativa leaves against the deleterious effect of hydrogen peroxide on some aspect of male reproduction in adult rats. Forty adult male rats were randomly assigned into four equal groups as follows: control group received tap water (untreated); group T1 were received tap water containing 0.5% H2O2; group T2 were received tap water containing 0.5% H2O2 plus administration of 300 mg/kg. B.W. ethanolic extract of Eruca sativa leaves and group T3 administration ethanolic extract of Eruca sativa leaves only at the same dose of group T2.
... Show MoreThis study is designed to assess the effectiveness of bovine urinary bladder submucosa on healing of cutaneous wound in Iraqi goats. A 32 (2X2) cm of full-thickness cutaneous wounds were induced in eight goats, two on each side of the lateral thoracic region. The wounds were divided into two equal groups (16 wounds/ group); treatment group included the wounds on the right side which were treated by covering the wound beds with strips of acellular sterilized bovine urinary bladder matrix. While, the wounds on the left side were left without any treatment (control group). The results were evaluated clinically (along) and histopathologically on 7, 14, 21, and 35 days post-inducing of wounds. The clinical evaluation of treated wounds showed
... Show MoreThe present study aims at using the biological techniques in a genotoxicity assessment of low doses of radiation in samples of workers in Al-Tuwaitha site due to decommissioning to radioactive contamination as a result of work. This study included 50 male blood samples, aged 35 - 63 years as well as 35 blood samples from non-smokers and non-alcoholic as control group which included 25 male and 10 female aged 25 -57 years. The endpoints used were sister chromatid exchange (SCE) and hypoxanthine guanine phosphoribosyl transferase (HPRT) mutation assay. The sister chromatid exchange in the human lymphocyte for radiation worker was significantly (P<0.05) higher than in the control group. While the results of mutat
... Show MoreThe present study was carried out to investigate the antibacterial activity of alcoholic
extracts of Plantago lanceolata leafes in vitro and in vivo by inducing urinary tract infection in
rats which caused by urethra administration of S.saprophyticus isolated from human and
animals(cow and sheep)
These extracts showed significant effect (P<0.05) on the inhibition of the growth of
S.saprophyticus in vitro with the superiority of the concentration 200mg / ml of alcoholic
extract with the mean of inhibition zone diameter 30 mm against S.saprophyticus ,while zone
diameter was ( 26.5 ,21 ) mm due to the concentration 150, 100mg/ml respectively.
This study included the therapeutic role of doses 150 mg/kg . B.W. of 1.
The antimicrobial activity of Lactobacilli has been widely exploited for prevention of
food –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially in
children, because of bacteriocin activity and the importance of herbal drugs, hence this study
was designed to evaluate the synergistic effect of plant extract and bacteriocin produced by
Lactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.
1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.
2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min and
then purified by two steps method including extraction with n-butanol followed by gel
filtration chromat
يعتبر التصنيع البايولوجي للسلينيوم النانوي أمن ورخيص وصديق للبيئه ومن المواد غير السامة وعلاوة على ذلك يكون السلينيوم النانوي مستقرا بسبب احتوا ئه على الغطاء الطبيعي من المواد العضويه والتي تمنع من حدوث تراكمه مع مرور الوقت , صممت هذه الدراسه للتحقق من التوافر البايولوجي لنانوات السلينيوم المحضرة بطريقه جديده باستعمال العكبر . تم استعمال 27 جرذا بالغا وقسمت
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