The relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb con
... Show MoreThis study was carried out in Artificial Insemination Center of Iraq to revealed FMD disease effect on some seminal attributer parameters of 14 imported Holstein bulls divided to three groups according to different reproductive efficiency (four High, five medium and five weak). Results showed that FMD disease had significant (P < 0.05) adverse effect on most seminal attributer parameters, mass, individual motility and sperm concentration / ml during post disease in first of two, four, all months of high, medium and weak semen quality bulls respectively .but semen volume didn’t influenced significantly with this disease. So semen collection should be suspended until resume normal fertility of sperm, after two, four month of high and
... Show MoreSTAG proteins, which are part of the cohesin complex and encoded by the STAG genes, are known as Irr1/Scc3 in yeast and as SA/STAG/stromalin in mammals. There are more variants as there are alternate splice sites, maybe three open reading frames (ORFs) code for three main proteins, including: SA1 (STAG1), SA2 (STAG2) and SA3 (STAG3). The cohesin protein complex has various essential roles in eukaryotic cell biology. This study compared the expression of the STAG1 gene in four different breast cancer cell lines, including: MCF-7, T-47D, MDA-MB-468, and MDA-MB-231 and normal breast tissue. RNA was extracted from these cell lines and mRNA was converted to cDNA, and then expression of the STAG1 gene was quantified by three sets of specific p
... Show MoreThis study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no
... Show MoreThe objective of this study was to evaluate the effects of platelet rich plasma and low level laser therapy (diode laser) on the healing of proximal sesamoid bone fracture. Eighteen healthy adult donkeys were divided into three equal groups: First group was used as control group, second group was treated by platelet rich plasma and the third group treated by diode laser. Also each group was divided to two equal subgroups according to period of postoperative macroscopical, histopathological examination: (one and three months post operation). All animals were exposed experimentally to transverse mid body fracture of lateral proximal sesamoid bone, in control group the bone fixed internally by screw and followed by external fixation with Pl
... Show MoreListeria monocytogenes has important medical health infection in human and animals. The present study aimed to investigate the significant ultrastructure changes occurred in liver post-infection by pathogenic strain of Listeria monocytogenes. Twenty-six mice of both sexes divided into two groups; 1st group (n=21) infected with 0.2 ml (2x109 CFU/ml) of Listeria monocytogenes I/P and 2nd group (n=5) served as negative control group, all animal of both groups sacrificed at 2, 4, 6 and 24 hours post infection (7 mice for each time of infected group). The histopathology of liver tissues post infection revealed degenerative changes and severe necrosis in hepatic lob
... Show MoreThis study was conducted on sixteen local Iraqi bucks aged 3 to 4 in College of Veterinary Medicine/ University of Baghdad from January to May, 2016. They were divided randomly into two equal groups; 1st group (G1) was treated by oral administration of 15 gm/daily cumin seeds for 3 weeks, while the 2nd group (G2) represented control group. The 1st group showed a significant (P≤0.05) increased in semen volume (2.08±0.36 ml), furthermore number of sperm was increased significantly also according to assessment of semen color (4.23±0.26). Mass and individual motility showed significant higher level in the 1st group (86.00±1.97, 87.00±1.62%, respective
... Show MoreThis study was conducted on 122 Arabi sheep belonged to private flock in Erbil plain Kurdistan region- Iraq, from July/ 2014 to August/ 2015 for studying the role of sex chromatin on daily milk production, total milk production, lactation period, fertility rate, prolificacy, birth weight and fertilizes estrous sequences. The repeatability for the studied traits were estimated. The ratio of sex chromatin shapes drum stick, sessile nodule, tear drop and small club were 23.37%, 60.22%, 8.54% and 7.87% respectively.The overall means of daily milk production, total milk production, lactation period, birth weight, fertilize estrous sequences, fertility rate, and Prolificacy were 814.65 g 81.49 kg 103.53 day, 78.10%, 1.
... Show MoreIn the present study, the investigation includes the estrus activity after synchronization of estrus by using 20 mg impregnated sponges with Medroxy Progesterone Acetate for 12 days with 400 IU I/M injection of pregnant mare serum gonadotropin 24 hrs. before sponges withdrawal. All 30 Iraqi sheep's showed (100%) estrus after sponge withdrawal. Twin percentage 40% while single percentage 60%. The polymorphisms of gonadotropin-releasing hormone receptor gene were analyzed as a genetic marker in 30 Iraqi sheep's. Two noticed genotypes (GA and GT), and single nucleotide polymorphisms in the exon2 region of gonadotropin-releasing hormone receptor gene as genetic marker and correlated with high litter size after hormon
... Show MoreCurrent investigation was carried out to explore structural developmental changes of the acculus rotundus in indigenous rabbits at different postnatal ages. To conduct that, morphological, histological and morphometrical approaches were employed on 24 rabbits at ages one, ten, fifteen and forty days. The findings indicated that in one day kits, the Sacculus rotundus cannot recognized with the naked eye but in the next advanced ages it appeared as expanded pear-like structure situated at the end of ileum. It opened into the 2nd gyrus at the base of cecum by saccorotundocecal orifice. Because it extends for 1-2 millimeters inside the lumen of the cecum, an annular ridge formed encircles the site of this
... Show MoreThis study was conducted to evaluate the effect of seasons on serum testosterone level, testis histology examination and testosterone receptors by using cytoimmunochemistry techniques. Samples of blood and both testes were collected from 24 local dogs aged between 2-3 years and weighted 18-22 kg. This study carried out at 2015, divided into four seasons. No differences in testosterone level were observed between seasons. However, histological examinations were characterized by significant (P<0.01) changes during seasons in tubular diameters, germinal thickness and tubular area. Cytoimmunochemistry investigation showed differences between seasons also, there was a distributions of testosterone hormone receptors
... Show MoreThis investigation was carried out to utilize common vetch Vicia sativa in formulating diets for 84 common carp Cyprinus carpio L. with initial weight 28±2g/fish were distributed randomly over seven experimental diets with three replications for each. The fish fed experimental diets for 56 days contained 30% and 45% of common vetch Vicia sativa of total diets which treated with germinated for 5 days (diet 2 and 3), 7 days (diet 4 and 5) and the last diets contained crude vetch (diet 6 and 7). The statistical analysis of data showed that the fish fed at diet 3 was significantly different (P≤0.05) compared to fish fed at another diets in total weight, total weight gain, relative growth ra
... Show MoreThe present study aimed to investigate the infection ratio and pathological lesions in common carp fish in Kirkuk province. To achieve these goals, a survey study was conducted from 01/12/2015 to 01/04/2016. Results of the survey study showed that the infection ratio of acidity and alkalinity of pond’s water 27.88% (46/165), bacterial kidney infection 20% (33/165), water mold (saprolegniasis) 12.12% (20/165), spring viremia of common carp 11.52% (19/165), Coccidiosis 11.52% (19/165), fungal gill rot infection 7.27% (12/165), hemorrhagic septicemia 6.67% (11/165) and enteric parasite infection 3.03% (3/165). Pathological study showed that lesions in acidic and alkaline of pond’s water consisted from necrosis w
... Show MoreThe present study was aimed to check the amount of variation in some hematologic and biochemical parameters accompanied with natural acquired canine dirofilariasis. Blood samples were collected from sixty five stray dogs (5-10) years old belong to local breed dog in the villages of Al-Hindya area/ Karbala Governorate, The affected animal showed differences in hematological and biochemical values as compared with reference ranges. In conclusion, the disease showed no significant clinical signs although many pathological changes in some blood constituent with serum biochemical parameters were observed, before euthanasia for dogs.
The aim of the study was found variation of melatonin receptor gene by sequence analysis, investigation in association between MTNR1A polymorphisms with any variation in allele’s frequency of the mutations C606T and G612A of the MTNR1A gene in the Iraqi local sheep lambing out of season. Blood samples were randomly collected from 60 ewes from Agriculture Research-Ruminant Researches Station Breeding Station, Baghdad. Genomic DNA was isolated using a Geneius TM Micro gDNA Extraction Kit. A large fragment of exon 2 of MTNR1A gene was amplified by PCR using specific primer pairs. The PCR product was sequencing 20 samples of MTNR1A gene was performed by National Instrumentation C
... Show MoreThe present study was carried out to focus light on the possible improvement and enhancement of functional performance of male mice sexual activities by using the aqueous extract of Crocus sativus. Several pharmaceutical drugs affect sexual activities and upset the sexual desire sequence that can lead to impotence or loss libido, example of these drugs are tricyclic antidepressant such as imipramine. To achieve this goal, twenty adult albino male mice were used, which were randomly divided into equal four experimental groups. The first group was given 10 mg/kg B.W. imipramine, the second group was given 100 mg/kg B.W. aqueous extract of Crocus sativus, and the third group was give
... Show MoreA total of 40 imported cream, butter, cheese and yoghurt samples were collected randomly at weekly intervals (1samples/ product/ week) from various supermarkets inside the Baghdad province during the summer period. A total of 84 samples of locally produced cream, Butter, soft cheese and yoghurt that belonged to buffaloes, cows and ewes were collected randomly at weekly intervals (1 sample/ product/ week) from three different townships (Al-mahmudiyah, Al-Rasheed and Al-latifiyah) and were examined on two periods, the first was the period that extended from the beginning of September to the end of November 2015 while the second was the period that extended from the beginning of January to the end of march 2016. Bes
... Show MoreThe present study was carried out to investigate the protective effect of Eruca sativa seeds against changes of pituitary and testes in cadmium treated rats. Forty adult male albino rats were used and divided into 4 equal groups and treated for 8 weeks as follow: The first group was given distilled water and used as a control group. The second group (G1) was administered tap water containing 30 ppm/ L of cadmium chloride. Animals in third group (G2) received tap water containing Cadmium chloride as in G1 plus 250 mg/Kg B.W ethanolic extract of Eruca sativa seeds, while rats in fourth group (G3) administered 250 mg/Kg B.W of ethanolic extract of Eruca sativa seeds only. After 8 weeks of
... Show MoreThe study was conducted to evaluate antitumor effects of green tea (Camellia sinensis) extracts (aqueous and methanolic) on Rhabdomyosarcoma; cell line and a normal cell line; mouse embryo fibroblast; Chemical detections of green tea extracts revealed that the aqueous and methanolic extracts were positive for flavonoids, alkaloids, phenol and glycosides. The percentage growth inhibition of five plant concentrations (50, 100, 250, 500 and 1000 µg/ml) were assessed in vitro using tumor cell lines Rhabdomyosarcoma and normal cell line mouse embryonic fibroblasts. The results revealed that the five concentrations of the plant extracts showed anti-tumor properties in a concentration-dependent manner
... Show MoreThe present study were made for analysis of major chemical and biochemical components of twenty hydatid cysts fluids include two group (10 fertile cysts and 10 sterile cysts) obtained from the lung and liver of 29 infected patients admitted to medicine city hospital for operation during a period of 6 month these components were measured by colorimetric assay kits (Biolabo®, Human). Results revealed marked (P<0.05).differences in the mean values, particularly among enzymes (Glutanicoxaloacetic transaminase, Glutanic pyruvic transaminase and Alkaline phosphatase), between fertile and sterile fluids while Lactate dehydrogenase level was exhibited high activities in fertile and sterile iso
... Show MoreThis study was conducted for the synthesis of silver nanoparticles by using olive leaves aqueous extract and evaluate its antibacterial activity against Pseudomonas aeruginosa in vitro. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible – spectrophotometer and Scanning Electron Microscopy. Well diffusion method was used to show the antibacterial action of silver nanoparticles against Pseudomonas aeruginosa in vitro in comparison with standard antibacterial silver sulfadiazine by using different concentrations of each agent ranged from 12.5-200 μg/ml. The results of this study showed it possible to produce silver nanoparticles in eco-frien
... Show MoreEffect of different concentrations of Nisin, Potassium sorbate and Sodium chloride on the keeping quality of the locally produced soft cheese samples against the Staphylococcus aureus viability was conducted during November 2015/ May 2016. Staphylococcus aureus were isolated from 20(73.33%)out of 30 bovine and ovine locally produced soft cheese samples and their identification were confirmed based on the morphological, rapid biochemical reactions and serological properties. Staphylococcus aureus isolates that obtained by plating on the Chromogenic agar were further tested serologically. The highest significant (P<0.05) prevalence levels of Staph aureus were found in the cow'
... Show MoreA total of 163 milk samples (500 ml each) of cows, ewe, goats, buffaloes, camels were collected randomly at weekly intervals (10 samples/ week) from both Abu-Ghraib and Al-Fudhailiya villages and also for different local retail markets inside the Baghdad province and these samples were including milk of different animal species, milk cans, bulk milk tanks and imported Ultra-high temperature processing milk. Among the total milk samples only 138 milk samples were examined during two climatic periods where the first period was in summer that extended from the beginning of September to the end of October 2015 while the second period was in winter that extended from the beginning of January to the end of Februa
... Show MoreThe present study was carried out during the period from November 2015 to April 2016 to determine the concentration of lead in Tigris River. A total of 75 fish of Hamri Carrasobarbus luteus and 82 specimen of common carp Cyprinus carpio were sampled at three different stations. Atomic absorption spectrometry is a technique for measuring quantities of Lead present in water and fish. The results showed that the mean concentration of lead in water more than in fish organs. Statistical analysis showed no significant differences in lead concentration between all stations. In C. luteus and C. carpio showed no significant differences in gills, muscles, liver and kidney between differe
... Show MoreIn this study a pyloroplasty technique was designed by using a single layer simple interrupted extra-mucosal suture pattern on pyloric region in dogs. Nine adult dogs were used to induce longitudinal surgical incision in the pyloric region under general anesthesia using a combination of 15mg/kg B.W. ketamine hydrochloride 5% and 5 mg/kg B.W Xylazine hydrochloride 2%. The incision was closed by pyloroplasty technique (longitudinal incision for whole layers with transverse suturing) using single layer simple interrupted extra mucosal technique. The examination achieved by daily clinical observation, radiological finding, at the end of 21st day post operation, macroscopical and histopathological examination at
... Show MoreFecal samples were collected from eleven animal species, representing, different species. Samples were collected from cages before daily cleaning, in clean container, the sample was homogenized in tube contain sterile saline and kept for 30 min. and transferring 1 ml of the supernatant into Sabouraud Dextrose broth and incubate at 25-30˚C for two weeks, then 0.1 ml were seeded in Petri dishes containing Sabouraud Dextrose agar with chloramphenicol (50 mg/l). Results indicates high percents isolate for Geotrichum candidium (70.90%) of all animals, the high percents was from Porcupines, Kangaroos, Camels, and ponies (100%), followed by Wolves, Lamas, Dogs, Monkys, Mountain goats, Deers and
... Show MoreThe study includes six adult healthy hen turkey. The cloaca has three compartments separated by two circular folds and next to rectum is the coprodeum which the largest and measured (2.5±0.2cm) in length and (2.1±0.1cm) in diameter. The middle and smallest is the urodeum that measured about (1.01±0.2cm) in length and (1.2±1cm.) in diameter, that receives the ureter and left oviduct. Posteriorly, the proctodeum that measured about (1.4±0.1cm) in length and (1.6±1cm.) in diameter. The coprodeum mucosa has short and flat villi. The urodeum mucosa has tall branching pyramidal mucosal villi. The mucosa of proctodeum has lost the villi pattern. The three compartments were lined with mucous secreting columnar cell
... Show MoreNewcastle disease is one of serious pathological problems and causes of vast economic losses during 2011-2016 in Iraq. The disease caused high mortalities in all types of poultry nevertheless of vaccination. In this study all samples were collected from infected flocks with clinical signs of the disease. Inoculation of chicken embryonated eggs was carried out for virus isolation, identification, Haemagglutination and Haemagglutination Inhibition assay. Using Reverse Transcriptase-Polymerase Chain Reaction to confirm the presence of the virus, Intra Cerebral Pathogenicity Index and Mean Death Time were used to confirm all the isolates that were velogenic. The important determinant of Newcastle disease virus pathog
... Show MoreThis study aimed to investigate the immune responses (Cellular and humoral) to Klebsiella pneumoniae and Eimeria tenella by using Delayed Type Hypersensitivity Test (Skin Test) and Tube Agglutination Test in sixteen local breed rabbits. Animals were divided into four equal groups; First group was immunized subcutaneously with 1 ml (1000 µg/ml) of sonicated Eimeria tenella oocysts –SETO-ET), second group was immunized subcutaneously with 1 ml (1000 µg/ml) of sonicated Whole Cells Klebsiella pneumoniae antigen –SKWC-KP), third group was immunized subcutaneously with 1 ml (500 µg/ml of sonicated Eimeria tenella –SETO-ET and 500 µg/ml SKWC-KP).
... Show MoreHydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015. Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder. The purification of mtDNA was done by (promega kit, USA). The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes was used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products. Polymerase chain reaction product for NADH1 800 basic pair. The polymerase chain reaction products were purified and partial sequences were generated. The sequences obtained were found to align with corresponding region for ND1 g
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