Aim of this research was quantitative detection of Increase and decrease in copy number of CaMV-35S promoter and Nos terminator in genetic modified tomato by using Real-time PCR. Twenty four samples of genetic modified tomato seeds were isolated from 84 tomato samples collected from Iraqi markets during the period from December 2016 to January 2017. The experiences were conducted in the Institute of Genetic Engineering, University of Baghdad. Go Taq®qPCR master mix kit supplied by USA Promega Company, three specific primers to CaMV-35S promoter, T-Nos terminator and β-actin housekeeping gene supplied by Canadian Alpha Company were used. To quantitative detection of increase and

A field experiment conduct during winter season 2022-2023. This study was aimed to investigate effect number of seeds.m² to two wheat varieties. The results showed the superiority of  V1 in most main stem growth traits (biological yield, number of ears .In square meters, plant height, spike length, spikelets number / ear and chlorophyll content ( 1.87 t. ha, 20.0 seed. m² , 113.90 cm , 18.40 cm, 26.37 spikelets/ ear  and 75.92 (SPAD ) respectively. V1 also excelled in all the traits of branch growth ( biological yield, number of peduncles /m², plant height, spike length and spikelets number / ear, (16.71 tons .Ha^-1, 259.17 ear /m²  ,104.57 cm, 16.83 cm and 23.6

Sustainable agriculture was adopted in field experiment in horticulture vegetable field during fall season 2013-2014, to study the effect of spraying nutrients and agral on cabbage hybrid yield, Split –split plot experimental as RCBD design with three replicates at 10 plants for each treatment was adopted, Tropicana and Redball (A and B) transplants (main plot) at 45 day age tranplanted to open field at 25/9/2013 plants, Vegeamino (T2) and Algaton 20 (T3) spraying with 1ml L^-1 three times for two week intervals for each of them while control plants (T1) spraying with distilled water (sub plot), plants covered (sub-sub plot) with agral C1 control plant (C0) (without agral), Results sh

The aim of this study which have been implemented in the AL-mada,an area 40 km southeast of Baghdad in the silty loam soil to evaluate of interaction effect between inoculant with biofertilizers and salicylic acid on Iron  and Manganese availability in soils and concentration in leaves and some vegetative growth indicators As well as flowers and quantitative specifications production quantity of plant Beans when irrigation with river and drainage water, the experiment Carried out by using Spilt-Spilt-Plot Design it was treatment of irrigation is the main plot while the treatment of spraying with salicylic acid is sub -plots plots while the treatment of bio-fertilizers are pl

A field experiment was conducted in Ehssayvillage-Amria-Fallujah district in Al-Anbar governorate during the spring season of 2012 and 2013 in order to study the effect of potassium fertilizer and foliar nutrition of iron and zinc in growth traits of two mung bean (Vigna radiata L.) genotypes. Factorial arrangement was used in RCBD with three replicates.The experiment included three levels of potassium (0, 60 and 120) kg K.ha^-1, four treatments of foliar application of zinc and iron  (0, Fe150, Zn100 and Fe150 + Zn100) mg.L^-1 and two mung bean genotype (Local and indian VC6089A10) .The local ge

This study was aimed to investigate straw stiffness in land races of two- row barley (hordeum vulgarel.). Straw from four landrace populations of barley grown in the Kurdistan Region of Iraq was measured and tested for bending stiffness, showing considerable variation in diameter and in longitudinal bending modulus. In the lower range of diameter, the straw from these landrace populations was highly flexible and was able to lodge without fracture, by curving elastically without fracture or buckling until the head reached the ground. The landrace populations studied contained substantial variation in internode diameter and in the stiffness of the wall material. Selection for internode diameter appeared to have potential f

This research was aimed at conducting a financial evaluation of sheep feeding systems in Al-Ahsa in KSA to identify the feasibility of investing in these projects. By field study, identified two systems for fattening the first system of fattening for 4 months, 3 cycles a year, and the second system of fattening for 6 months, two cycles a year. Secondary data collected by the Ministry of Environment, Water and Agriculture, the annual statistical book, a random sample of sheep breeders in Al-Ahsa governorate. The criteria for financial evaluation of projects are used. The results showed that the system of fattening for 4 months better than the other system, where the internal rate of return (IRR) f

Pot experiment was carried out at a greenhouse belongs to College of Education-University of Alanbar during winter season of 2013/2014 to investigate the effect of application of different levels of bat guano 0, 4 and 8 gm on some growth traits for two bread wheat triticum aestivum L. Cvs, one is local cv. (Aliz) another one is imported from Australia (Eratom). The experimental treatments were distributed under with factorial experiment arrangement 23 and 43.30 gm and root dry weight of 7.29 and 7.53, for each combination, respectively. It thus could be concluded that the application of bat guano at 8gm alone was the better than 4gm in some traits. However, the genetic affinity of local cv was dominated with 4gm applicat

Field experiment was conducted out Al-Anbar in winter season of 2011/2012 to investigate decision making of selection to the genotypes of caraway Carum carvi L. thus, to introduce in consequent breeding program and test effectiveness of GT Biplot and two model of multi-trait selection, master and against checks jointly in GGE Biplot software for seven genotypes by six traits of caraway. Genotypes were randomly distributed underwith RCBD design with three replicates. Results revealed that these techniques were effective to derived information from data. Where, GT Biplot extracted 92.6% of total variation. Furthermore, it was indicated that was significant association between plant height and umbellets no. which those trai

A field experiment was conducted at the experimental field, Department of Field Crop - College of  Agriculture Abu-Ghraib- University of Baghdad, during summer season at 2012 and 2013, to determine the response of  growth analysis parameters in some cotton cultivars to Integrated Weed Management. A randomized complete block design (RCBD) arranged according to split-plot was used with three replicates, included six weed control treatments as Main-plots where as Sub-plots included eight cotton cultivars. The results showed various to response of  growth analysis parameters to weed control treatments , the treatment o

Two experiments were conducted to study the impact of adding partial acidulated rock phosphate in comparison with traditional phosphate fertilizers. First experiment was conducted to study Kinetics of P release from partially acidulated pock phosphate (30, 40, 50, 60, and 70%) or from TSP and SSP at 80 kg P ha^-1. The results showed that the release of phosphorous from acidified rock phosphate was characterized by 1^st  order kinetics with high (R²) and lowest SEe. TSP gave the highest quantity of soluble phosphate in aliquot compared to SSP. The 2^nd experiment was a field one aimed

Al shehemia project in Wasit Governorate was selected to represent the Irrigated area in middle Iraq to study the temporal change in land suitability for wheat cultivation. Soils of the study area consist mainly, of ntisols and Aridisols soil orders .Historical Soil data were  collected from previous  works  done in 1972 . and some soil propertic were reavoluoted at 2014. Soil and climate were evaluated for wheat cultivation according to Sys ,et al system (1993) and FAO, (1976) which developed by the Italian institute in  2012. The results showed that most of soil units are highly suitable for wheat growing with class S1 and consists about %85.34 from the total area, and %10.90 for N1and N2 classes. Land suita

This research was conducted in the greenhouse of Al-Rabee Research Station of the Department of Horticulture, Ministry of Agriculture for the 2011-2012 season in order to examine the effect of foliar application of various combinations of polyethylene glycol and salicylic acid levels on some vegetative growth traits of date palm tissue cultured derived plants.The concentrations of polyethylene (zero, 20 and 40 mg.L^-1) and salicylic acid (zero, 50 and 100mg.L^-1). The experiment carried out using randomized complete design and factorial in three replications. The results showed that increasing the concentration of polyethylene led to increased paperwork content of chlorophyll B and the total and received an in

This experiment Carried out at the Dept. of Research and Studies– office of Horticulture- Ministry of Agriculture at Abu Ghraib, for two seasons in 2014 and 2015. The objective of the experiment  to stady the effect of electric shocks on some okra (Abelmoschus esculentus L. Moench) traits  study. Six pure lines (S1 , S2, , S3 , S4 , S5 , S6) were attached  with four levels of electric shock (0, 9, 12 and 15 amp for 5 minutes) The 24 treatments were planted according to the RCBD with tow replicates to produce plants of the first generation  on which the selection is to get the seeds of the second generation. A factorial experiment within RCBD with three replicates, were used  in the pla

Field experiment was conducted during 2015 spring season on Okra(Abelmoschus  esculentus L.Moench) grown under protected cultivation in the Research and Studies Department - Horticulture Office at Abu Ghraib, to study combining ability and some genetic parameters. Six pure lines ( S1 , S2, , S3 , S4 , S5 , S6 ) were crosses using full diallel crosses. The crosses , parents and hybrid H-597 compared , using RCBD, with tow replicates . Results showed that the promise hybrid in the yield per plant and pods number and the number of flowers was S1 × S3, followed by hybrids S3 × S6 and S5 × S3. The gene action for number of flowers and number of pods in diallel and reciprocal hybrids and the early flowering and yield p

This research was conducted during spring and fall seasons, 2015 , at the fields of Field Crop Department – College of Agriculture –University of Baghdad . The objective was to study the effect of grain moisture at  harvesting on some agronomic traits  of the corn  next  generation , by  using synthetic variety 5018 . In spring season 2015 , seeds of this variety  was planted ,when  moisture of the ear grains was (37-42%) , ten ears were harvested. Ear harvesting dates were performed manually when the grains had 37-42% , 34-36% , 30-33% , 25- 28% and 19-22%  moisture content. In fall season  2015 , varietal trail was carried out to the five treatment materials , us

Common Buckwheat (Fagopyrum esculentum Moench) is recognized as a healthy food in many countries because it is nutrient-rich crop. The objectives of this study was to investigate  the succesful farming for buckwheat plants in the Iraqi environmental conditions, extraction and estimation of the most important nutritional and medicinal compounds from seeds plant and it᾽s effectiveness in the inhibition of lipids peroxidation which considered to be the causative agent of the free radicals formation in vivo.The hexane and methanolic extraction gave many compounds from buckwheat seeds. NMR were used to determine many of the natural compounds for buckwheat plants seeds after ma

A field trial was conducted at Abu-Ghraib Research Station-Agricultural Research Directorate-Ministry of Agriculture during the winter seasons of 2012-2013 and 2013-2014. The objective was to investigate the effect of source-sink Regulation on grain yield  and its companents of five bread wheat cultivars (Triticum aestivum L.( Randomized complete block design with the arrangement of split-plots with three replicates was used. Bread wheat cultivars (Bohooth-22, IPA 99, Abu-Ghraib-3,Al-Fath and Al-Rasheed) occupied the main plots while treatments of source-sink relationship modification occupied the sub-plots. They included eight treatments: Control (Con.), S1 (remova

The relation between communication activities of extension agents and the applications of wheat growers to the wheat agricultural recommendations in Kirkuk, The main goal of this study was to determine it communications activities of extension agents correlated with the applications of wheat growers to wheat agricultural recommendations in the province of Kirkuk, Questionnaire was designed with to parts to achieve this study, The first part had (24) items related to communication activities (divided to four groups) and the second parts had eight item related to wheat agricultural recommendations stratified sample was used to drown the sample for 17 agriculture divisions in Kirkuk, The final sample had 150 growers from there divis

The aim of this study was to investigate the effect of replacement of low density Lipoproteins (LDL) in different concentrations  ,exctracted fram egg yolk in the Tris diluents of the Holestion Freizan bulls semen after cooling and different cryopreservation periods . This study was done during the period From September/2014 to March/2015 and a two parts periods . First from September to November /2014 to done the extraction of the low density Lipoprotein which it have be succifully  done at the viruses Laportary  which belonge to the diagnosis department /state Board of the plant protection /Ministry of Agriclturd (Abu_Gruiab/west of Baghdad).The LDL extraction was done by a procedure with different steps from fre

The study was conducted for a period 120 days of metal floating cages were used 2.25 × 2.25 × 1.5 m dimensions, for breeding two types of carp fish and common carp Cyprinus carpio.L and silver carp Hypophthalmichthys molitrix rate of 240 fish (120 common carp +120 Silver carp) for each treatment in three replications, and made laboratory four diets with obrocana content of between 27.83% and 28.52% and energy between 439.39 and 445.68 Kilusarh/100 g as it was the bush first free any addition treatment called the second control and treatment contained vitamin E (500 mg/kg) and third-treatment contained linseed oil by 1% and the treatment represented a fourth of all oil and vitamin mentioned the same proportions

This study was included investigation of the resistance genes Mi which is responsible for the appearance of resistance feature in tomato against root knots Nematode Meloidogyne spp in 46 pure lines of tomato indeterminate growth, ten of tomato pure lines showed resistance to root knots nematode infection after 60 days of the inoculation by 5000 egg/ kg of soil . While 31 pure lines of tomato showed sensitivity for these nematode infection, which root knot  numbers had exceeded 100 knots. Then the genes has diagnosed by using the technology of molecular analysis the DNA of the purelines of sensitive and resistance tomato was isolated by the CTAB method then apply the indicators of Si

This study was undertaken to evaluate the effect of silver zeolite (SZ) on hematological parameters and survival rate in common carp, Cyprinus carpio infected with Saprolegnia spp. A total of 120 fish (weight 100 g) were divided into six treated groups in duplicate (10 fish/tank) as follows: T1, T2, T3 and T4 that infected with Saprolegnia spp. (2×10⁴ zoospore/ml) and treated with SZ at (100,200 and 300 mg/l) while T4 infected with Saprolegnia spp. and treated with copper sulfate )5g/100 L(; C+ group, fish were infected with Saprolegnia without treatment and C- served as control negative group. Following 14 days from infection with Saprolegnia s

MicroRNAs are non-coding gene regulators that may serve as systemic lupus erythematosus biomarkers for diagnosis or prognosis. This study was aimed to identify the single nucleotide polymorphism (rs1140713) of microRNA-126 and its expression by using PCR and qRT-PCR techniques. This research involved 100 womenwith SLE with a mean age of 32.85±9.992 years they were obtained from Baghdad city (medical city, Baghdad Hospital)/Iraq, along with 100 healthy controls with a mean age of 31.68±8.308 years. The mean score on the SLE disease activity index (SLEDAI-2k) was 10.860.±3.275  and a disease duration was 9.00 years, (Erythrocyte sedimentation rate, C-reactive protein, urea, and creatinine w

This experiment was carried out at a private field in the eastern Radwaniyah Baghdad for the fall season 2020/2021 and spring 2021 to study the effects of adding mineral fertilizers, spraying salicylic acid and amino acids on some growth traits and yield of industrial potato plants. 200 kg N h^-1 , 100 kg P₂O₅ h^-1, 100 kg K₂O h^-1 and F₂ consist of 275 kg N h^-1, 180 kg P₂O₅ h^-1, 200 K₂O h^-1 and F₃ consist of 350 kg N h^-1, 360 kg P₂O₅ h^-1, 300 K₂O h^-1 and salicylic acid in three conc

The objective of this research was to study the chemical composition and functional properties of flour and protein isolate from mung bean by isoelectric precipitation. Moisture, protein, fat, ash, crude fiber and carbohydrate were 5.88%,  24.52%, 1.24%, 3.33%, 5.22% and 59.81%  for flour and 2.48%, 83.70%, 0.24%, 2.48%, 0.52% and 10.58%  for  isolated protein  respectively. The results of functional properties revealed an increase in solubility rates by increasing the pH values of both flour and isolated protein, the mung bean flour showed higher solubility than protein isolate. The foam capacity of flour was higher than that of protein isolate, however foam stability for protein isolate reached 23.53%,&

A field study was performed in the Agricultural Machines and Equipment Department, Agriculture College, Basrah University, to study the effect of the operating pressure and nozzle height on droplet properties reaching to the target using knapsack sprayer with a maximum capacity of 16l. A nozzle is mounted on the rod of sprayer. Three different operating pressures were tested in this study of 1, 1.7, 2 bar and three different nozzle heights of 50, 70, 90cm. Brilliant Sulpho-Flavinedye was used to measure mean droplet sizes, droplet densities, droplets number, and relative span factor deposited on white card papers. The spray properties were determined after spraying in field and then were analyzed using Image J software. The main

The study was carried out in order to determine the degree of genetic kinship among fifteen barley genotypes using SSR technique with 14 pairs of primers. The study showed that, all the primers gave amplification products and showed polymorphism between the studied genotypes. The total number of alleles was 19, with an average of 1.35, and the number of polymorphic alleles was 19, the percentage of polymorphism was 100%. The number of total and polymorphic alleles ranged between one allele as the lowest value for primers (Bmac0209, Bmac0067, Bmag0225, Bmag012, Bmag0394, Bmag0353, Bmag0206, Bmac0031, GBM1362, EBmac0603) and three alleles as the highest value for the primer Bmag0006. The polymorphism information co

This study was aimed to identify the obstacles that facing cereal farmers that applying the agricultural practices to decrease the effects of climate variation phenomenon in the central region of Iraq. This research was conducted on cereal farmers at Baghdad, Najaf, Babil, and Wasit which were the most cultivated governorates of cereal crops (wheat, rice, and yellow corn). A randomized sample of (227) farmers was taken, that represents 0.25% of the total cereal farmers in the four governorates (90,584 farmers). In order to achieve the research goal, a questionnaire was prepared to collect data related to the research topic, for which a four-way scale was designed (33) paragraphs, and this field included three axe

This research was aimed to estimate the long-run cost and supply functions and to calculate the optimum profit-maximizing level of production, optimum capacity for broiler projects. The preliminary data were obtained by questionnaires that were distributed to project owners in Qadisiyah, Babil and Wasit governorates. A total of 80 projects amounted for 15% of the total projects in these governorates were included. The results indicated that the optimal profit-maximizing and actual production were 25,337, 34,737, and 21.25 tons respectively. The optimum production capacity was 9.9 thousand birds, while the actual capacity was 8.3 thousand chicken The cost elasticities were 0.936, 1.0 and 1.23 at the actual, optimal and profit maxi