The present investigation was carried out to investigate the antibacterial efficiency of silver nanoparticles (AgNPs) in vitro against Aeromonas hydrophila using disc diffusion assay and minimum inhibitory concentration. The pathogenic A. hydrophila was isolated from infected common carp, usually diagnosed by chemical methods, and Avitek 2 compact device were used to confirm the diagnosis. The effectiveness of the prepared AgNPs was tested by chemical and biological (green synthesis using lemon extract) methods and were diagnosed by Fourier-transform infrared spectroscopy (FTIR), UV-Visible spectroscopy, Transmission electron mi

The current study was designed for analysis of the pattern of the nucleotide sequence of the tissue DNA isolates based on the ITS2,28SrDNA gene by the traditional polymerase chain reaction. All amplicons were well suited for the prepared primer with size 873 bp . and identical ratio ranged 87.2- 98% of the same species, as included a high similarity of the isolates taken camels in Iran and Iraq related to Parabronema skrjabini . Phylogenic tree inferred the degree of relatedness between 28SrDNA sequences deposited in the international nucleotide bank sequence database (NCBI). The sequence of Parabronema skrjabini was recorded in the Genbank under accession number MT742154.1.

The current study was aimed to investigate the antibacterial and antibiofilm activity of ethanolic extract of Hibiscus sabdariffa L. calyx against locally isolated methicillin-resistant Staphylococcus aureus (MRSA) at different concentrations were started from 0.078 to 40 mg/ml. The extract was prepared by soaked calyces powder of Hibiscus sabdariffa L. with 80% ethanol in the Soxhlet extraction unit, and then it was aseptically filtered. The antibacterial activity was tested by agar diffusion method and broth microdilution method, this method was used to determine the minimum inhibitory concentration of extract, while the antibiofilm activity was determined by using 96-well polystyrene microtiter plate

The following study was conducted to investigate the correlation between the expression of three different genes (NOB1, DDX47, CD101 (with the occurrence and development of chronic myeloid leukemia (CML) in Iraq. The difference in the expression of these genes between patients and healthy controls was studied. Moreover the correlation of age and gender with CML occurrence and comparing with control was also examined. Results showed significant increases in mean of gene expression level (ΔCt) of patient groups for all genes compared to the corresponding ΔCt means in control group, also the gene expression folding (

Horizontal porous pipe method is one of the most efficient systems of irrigation in arid and semi-arid areas.  The main aim of this study is to simulate the subsurface horizontal porous pipe irrigation under different conditions.  By this method of irrigation, an optimum amount of water is reached to the crop.  Moreover, it saves more water than the other irrigation systems.  Simulation models by HYDRUS/2D  are described the distribution of wetting shapes in two different soil textures through the system of United States Department of Agriculture, USDA, namely as loam and silt soils.  The system is designed for three diameters of 6, 7, and 8 cm insta

Meropenem (MER) antibiotic is removed from an aqueous solution using Mg-Al-layered double hydroxide (Mg/Al-LDHs) which serves to functionalize the principal object of enquiry in this paper–sunflower husks (SFH).  The former sample demonstrated a 51% removal of MER while the latter demonstrated a 67% removal of MER. One of the areas explored was the impact of the rise calcination temperatures resulted in a concurrent increase in the surface area and porosity of LDHs which, in turn, provoked a heightened removal efficacy of MER at calcination temperatures between 300 °C and 350 °C. Nonetheless, LDHs experienced structural deformation and thus, diminished removal efficacy of MER when the ca

There is an elevated need for novel antimicrobial preservatives in the food industry, and hydrolysis of waste products from the same sector has for decades been viewed as a potential source of these. In the current study we have purified bovine whey protein using size-exclusion chromatography (SEC), and hydrolyzed it by trypsin, pepsin, alcalase, savinase, and neutrase at different times 30, 60, 120, 240, and 300 min. The highest active time hydrolysate was subsequently fractionated by SEC, monitored for antibacterial and antioxidant activities, and characterized using UHPLC-MS/MS. Alcalase and savinase displayed higher degree of hydrolysis, higher antibacterial activity in their hydrolysates at 60 and 30 min, respectively compar

This study was aimed to evaluate the Aqua program in calibration and validity and its use in simulation to study the productive and water characteristics of maize crop.. The experiment was conducted in two irrigation methods, sprinkler irrigation and surface irrigation, and two cultivation methods are borders (lines) and furrows (lines and then furrows) and for two cultivars of maize, a hybrid (unlocal) and a local variety. The results that sprinkler irrigation reduced water consumption compared to surface irrigation, as it reached (558.38) mm for sprinkler irrigation, and (668.79) mm for surface irrigation. While the method of furrows after borders outperformed the borders only in improving growth characteristics and increasing

A field experiment was carried out at Agricultural Researchs and the Experiments Station in Wasit province during 2019-2020 season. This study was aimed to investigate the effect of three factors, the first being biofertilizer at three levels (without addition , a combination of Bacillus megaterium + Glomus mosseae and complet combination of G. mosseae, Azotobacter chroococcum, B. megaterium and Azospirillum brasilense). the second factor was three levels of vermicompost (without,3,6 Mg ha^-1).The third factor included three levels of triple superphosphate (0, 40 and 80%) of the fertilizer recommendation. The treatment were distributed in a factorial experiment according to a randomized complete block

This study was carried out in the laboratory to investigate the effect of addition of different levels of formic acid (FA) and urea on chemical composition and fermentation characteristics of common reed silages. About 400-500g of silage samples were prepared by treating chopped reed plants (2-3 cm) with solutions containing 10% of date juice, 0.5, 1 or 1.5% of FM and 0, 1 or 2% of urea. Silage samples were packed in double nylon bags and stored anaerobically for 60 days. Results showed that green color was roughly dominant in most samples of silage with vinegar odor. Samples were well aggregated with little mold was observed in few urea untreated silages. Results revealed also that increasing le

The present study was conducted at the nursery of the College of Agricultural Engineering Sciences; University of Duhok, Kurdistan Region, Iraq, during growing season (2020) to evaluate the effects of foliar sprays of three concentrations of aminoplasmal (0, 50 and 100 ml.L^-1), concentrations of humic acid (0, 100 and 200 ml.L^-1) and boron (0, 50 and 100 mg.L^-1) on vegetative growth and nutritional status of one year old almond seedling (Prunus amygdalus). The factorial experiment within randomized complete block design was used with three replicates. Foliar spraying applied at two times. First spray was carried out on 25^th of April and the oth

This study was aimed to recognize the spatial and temporal features of urban development and its impact on the climate of Baghdad City. The analytical method of the study relies on changes in Land Use/Land Cover (LULC), Normalized Difference Vegetation Index (NDVI), Normalized Difference Built-up Index (NDBI), and Land Surface Temperature(LST); GIS technology was used to measure these statistics. Landsat (5,8) and Sentinel2A imagery were used to detect the change of urbanization growth, vegetation change, and land surface temperature during the study period from 2001 to 2020, whereas used the supervised classification technique for determination for LULC variations. The results showed significant changes among the LULC classes in

A simple, cost-effective, and environmentally friendly method for making green nanoparticles has been developed. The current study examines the use of M. communis leaves extract in the biosynthesis of copper nanoparticles (CuNPs). The color change and UV-visible spectrophotometer, which showed a distinct peak at 481nm, confirmed the formation of these particles. Different techniques were used to characterize copper nanoparticles. The influential functional groups that can bio-reduce the copper ion Cu²⁺ were identified using Fourier Transform Infrared (FT-IR) spectroscopy. The crystal structure of copper nanoparticles was determined using X-ray diffraction (XRD), as evidenced by the peaks at 2θ values of 43.35

This study was aimed to investigate active compounds in the aqueous extract of Ganoderma applanatum mycelium. The aqueous extract of fungus mycelium was prepared and some active compounds were detected, including phenols, flavonoids, tannins, alkaloids, glycosides and resins, all of which gave a positive detection. The effective compounds were diagnosed by GC-Mass Chromatography technique, was revealed that there are a number of compounds that can produce mycelium with ability to inhibit microorganisms, inhibit free radicals and prevent oxidation, as well as their effect in reducing cancerous tumors. As for the effective groups, they were diagnosed by FTIR technique.

This study was aimed to estimate the domestic demand function on tea imports in Iraq using the Autoregressive Distributed Lag model (ARDL) for co-integration during the period 1990-2020.The data are verified for stationary by using unit root tests basis on Augmented Dickey - Fuller Test. The existence of a long-run equilibrium relationship among tea import quantity, current prices of tea and coffee buying, gross national income, and number of buyers is proved using bounds test method to co-integration. Results of estimating short and long-run models of the demand of tea imports by using the unrestricted error correction approach showed that the error correction parameter is adjusted annually by 140%. Results of estimating price e

This research was implemented at vegetable field of the College of Agricultural Engineering Sciences - University of Baghdad during fall seasons, 2018 and 2019. The experiment was conducted using factorial within Randomized Complete Block Design arrangement with three factors and replicates (3X3X2). The aqueous barley sprouts extract (B0, B1) (0, 100 g.L^-1) represented the first factor. Trehalose (T0, T1, T2) (0, 50, 75 mmole.L^-1) represented the second factor. Calcium (C0, C1, C2) (0, 1, 2 ml.L^-1) represented the third factor. The research objectives are assessing the impact of the mentioned factors and their interactions on carrot plant minerals

This study was conducted to evaluate the effect of contaminated soil with lead and chromium using three species of trees Platanus orientalis, Eucalyptus camaldulensis, and Populus nigra as a remediation tool which considers as a priority restoring method to keep healthy soil which support life system for all organisms. The experiment was conducted with three concentrations of Pb (200,400, and 600 mg.kg^-1) and three levels of Cr (100, 200, and 300 mg.kg^-1) along with control. The results reveals that root dry weight for orientale plane and poplar tree were reduced meanwhile, eucalyptus showed significant increase at 200 and 400 mg.kg^-1 of lead. Shoot dry weight of

This study investigates the efficacy of sand coated with calcium / aluminum (Ca/Al)-layered double hydroxide (LDH) in the elimination of methyl orange dye from simulated groundwater using method of permeable reactive barrier. For green (sustainable) manufacturing of this sand, aluminum prepared from dissolution of alum (cheap coagulant) can be interacted chemically with calcium extracted from cement kiln dust byproduct to create nanoparticles of Ca and Al that precipitate on the filter sand. Sorption measurements for interaction of methyl orange dye and coated sand was well formulated by Langmuir model with highest value of correlation (determination coefficient= 0.999) and lowest errors (sum of squared errors= 0.0122). Also, the

This study was aimed to produce potato minitubers via tissue culture technique by testing four cultivars; Agria, Nicola, SM 12-124-15 and JIP 1600-1 under in vitro, greenhouse and open-air field conditions. Nicola cultivar was superior upon the other cultivars under in vitro and greenhouse conditions by producing the best shoot multiplication (1.89 shoots/ explant and 13..27 leaves/ explant) and the highest number of minitubers (8.87 minitubers/ plant) in greenhouse whereas, SM 12-124-15 was better in the open field conditions by producing 11.25 tubers per plant, recording the highest mean weight of tubers (321.33 g), the highest weight of tubers per plant (3.615 kg), the bigger sizes of tubers (length 8.22 cm:

This study was aimed to purified and characterized  the  bacteriocin produced from Lactobacillus acidophilus HT1,  in order to use it in a skin pharmaceutical formula. The optimal conditions for bacteriocin production was investigated and results showed that modified nutrient broth was the best medium with glucose (30 gm/L) and yeast extract (7 gm/L) with peptone (7 gm/L) were the optimum carbon and nitrogen sources. In addition, 2% inoculum size, 37C◦ and pH 6.4 were the optimal conditions to obtain maximum bacteriocin

This research was aimed to study the management of water resources within international basins, among which is the study of water authorities within the Greater Zab River Basin (GZRB) in the Kurdistan region - Iraq, where it is an international river shared by Iraq and Turkey, and aims to develop the river basin management and international cooperation between the authorities Shared water within the basin, as well as identifying factors that affect implementation, the principles, and joint projects. On the other hand, the interactions of water users competing for water inside the river basin were identified. For this reason, the Water Evaluation Analysis Planning (WEAP) model was applied to the basin to determine the optimal GZR

This study was aimed to evaluate the ability of local oyster mushroom(Pleurotus ostreatus; ID: MF065715.1; cultivated until maturation in growth medium containing cumin extract) to modulate the genotoxicity induced by cyclophosphamide (CP) in bone marrow cells of mice. In vivo genotoxicity was assessed by quantifying the incidence of micronucleated polychromatic erythrocytes in the bone marrow cells of mice that were administered different doses of P.ostreatus extract (150, 200,250, and 300 mg/kg/day( respectively, individually, and in combination with CP (40 mg/kg body weight) according to the following three experimental protocols (pre 2h, post 2h, and concomitant treatment for 14 day

This study was aimed to evaluate the influence of Human Leukocyte Antigens- G (HLA-G) gene polymorphisms on the risk of rheumatoid arthritis (RA). In the present study, blood samples were collected from 100 rheumatoid arthritis patients and 100 healthy controls. Serum levels of HLA-G in both patients and controls were measured using ELISA kit which was shown a higher significant differences (p< 0.05) in comparison between them (539.3 ng/ml and 139.3ng/ml) respectively.  The frequency of the allele and genotypes in the patient groups and control groups were determined using  Polymerase Chain Reaction (PCR) for 14 bp polymorhisms in exon 8 (rs66554220) while direct sequencing analysis was done to dete

This study evaluated the CRISP2 gene's functional single nucleotide polymorphisms, and its results may be advantageous for future population-based studies and early diagnostic discoveries, particularly in developing effective treatments. The CRISP2 gene encodes a secretory protein with a high cysteine content, which belongs to the family of cysteine-rich secretory proteins (CRISP). SNPs are genetic variations that may affect a protein's structure or functionality. Prior to carrying out a broader population investigation, it is possible to evaluate suspected functional SNPs since it is challenging to uncover functional SNPs in disease-linked genes. As a result, using various bioinformatic predict

A factorial in randomized complete block design (RCBD), with three replicates, including three seeding rates (200,250, and300seeds.m^-2), and three grain size grades of Admiral variety of triticale whole (ungraded), thousand seed weight (TSW) 47.32g, heavy seeds 54.04g and light seeds 40.18g; for two successive growing seasons 2019-2020 and 2020-2021. The results revealed that spike length resembled number of spikelets per spike, grain density and number of grain per spike as seeding rate 200 with whole seeds attained highest value. Great differences between the two seasons were obvious, which was attributed to shortage in rainfall in the second season(407.1mm and 165.4

This study set out to screen 36 common plants which have the greatest level of laccase enzyme activity. It revealed that the enzymatic activity of fenugreek seeds was the highest in comparison with other plants. The optimal enzyme-specific activity was 5340.38 units per milligram protein which were obtained by extracting the enzyme with a sodium phosphate buffer at a concentration of 0.02 M and pH 8.0, at a ratio of 1:40 (weight to volume), and extracting time of 210 minutes. The enzyme yield was 27.6% after extraction and purification by gel filtration using Sephacryl S-3 after 1.01 purification fold. The optimum circumstances for enzymatic activity and stability were found by using 0.1 M sodium acetate as a buffer at pH 5.

This study was conducted to identify the polymorphism of ATP1A1 gene, distribution ratios, and its association with semen characteristics of Holstein bulls. Twenty-five Holstein bulls were used currently. Blood samples were collected via venipuncture to separate the DNA and identify the polymorphism of the ATP1A1 gene. Semen was collected using an artificial vagina for fresh evaluation. Results were showed the change of cytosine (C) nucleotide at site 15738 to adenine (A) at the exon 17 (SNP C15738A) of the ATP1A1 gene. Two polymorphisms were obtained according to this change, the first is wild (CC; allele frequency 0.84), and the secon

This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection  from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabili

This experiment was aimed to evaluate the effect of amalgamated NPS chemical fertilizer on crop performance and nodulation of soybean (Glycine max L (Merrill)) varieties on acidic soil during 2020 cropping season. Varieties of soybean i.e “Katta” and “Korme” and six levels of amalgamated NPS chemical fertilizer were used in factorial arrangement within randomized complete block design with three replications. Nodule parameters were highly influenced (P<.001) by the main effect of amalgamated NPS chemical fertilizer levels. The highest grain yield (2390 kg ha^-1) was obtained from application of 19(N) 38(P₂O) 7(S), kg ha^-1 .. “Katta”and “Korme” varieties

A field experiment was carried out during  the spring season 2020 at the University of Baghdad to study  the effect of the length and depth of the drip tape on  wetting  advance ofsubsurface drip irrigation system using Hydrus 2D/3D program. The site was planted with sunflower  in a clay loam texture. The drip tape was installed at 0.3m below soil surfacein two lengths; 20m and 40m. The experiment was designed according to the arrangement of split plotsdesign and  with three replications. The treatments were randomly distributed and planted with sunflower crop. The results showed that the moisture contents of the 20 mlength drip tape was higher compared with  the 40 m   drip tape lengt