The objective of the current study was to investigate the influence of adding resveratrol (Res) to a milk-based extender on some post-thawed semen quality of Iraqi buffalo bulls for different preservation periods (cooling at 5˚C, 48 h, 1, and 2 months post-cryopreservation; PC). Four bulls, 3.5 to 4 years old, were used from 11th November 2020 to 1st June 2021. Semen samples were collected, pooled, and divided into four groups using different Res concentrations (0, 100, 150, and 200 μM) added to the milk extender. The Res-150 μM group exhibited higher (P≤0.01) plasma membrane, acrosome integrity percentages, and total antioxidants concentration (TAC) compared to the other groups. Sperm abnormalities and DNA damage percentage
... Show MoreNickel is an essential nutrient for plant growth with low concentrations, its excessive amounts in soil above threshold values could be cause in toxicity. The main objectives of the present research were to determine the effects of nickel nanoparticles foliar spray with 20, 40 and 70 nm diameter on the physiological characters and anatomical aspects of Phaseolus vulgaris L. plants. Lowest reduction significantly (P < 0.01) in root and shoot biomass was recorded due to in 70 nm; the measurements 0.08 and 0.05 g per plant and highest root: shoot; 0.65 as compared with control treatment. As well as the lowest conserved water content; 40% was observe
... Show MoreThe present study was aimed to test the antagonistic activity of endophytic fungi isolated from Aloe vera leaves against a number of plant-pathogenic fungi. The results showed that the highest antagonistic activity was exerted by Penicillium chermesinum against the pathogenic fungus Rhizoctonia solani, with an inhibition rate of 78.57%. Also, Talaromyces verruculosus and P. chermesinum showed their highest antagonistic activities against pathogenic fungus Fusarium 1 with inhibition rates of 61.79% and 61.53%, respectively, while T. verruculosus showed an inhibition rate of 62.91% against the pathogenic fun
... Show MoreThe experiment was conducted using Potato( Solanum tuberosum L.) at the eastern Radwaniyah at private field during fall season 2020/2021 and spring 2021 to study the effect of nitrogen levels to 350, 275, 200 kg N h-1 ( N1, N2, N3) and phosphorous to 100, 180, 360 kg P2O5 h-1 ( P1, P2, P3) and potassium to 100, 200, 300 kg K2O h-1 ( K1, K2, K3) to vegetative growth and yield of industrial potato, The seeds of the hybrid potato Sinora, Class A, were planted in the fall season on 15/9/2020 and Elite in the spri
... Show MoreThis study was aimed to evaluate the effect of sublethal concentrations of lead chloride on physiological and histopathological studies in Gambusia affinis . A total of 500 fish were collected from the bank of the river east of Mosul city. The fish in the aquarium were divided into five groups (control, acute exposure(20,25mg/l) and chronic exposure(5,10mg/l)). The bioaccumulation of lead in acute and chronic periods of exposure showed a significant difference in values between control and treatment. The greatest level was seen in the kidney of fish treated with lead dichloride at 10 mg/l for 30 days. The level of antioxidant Glutathione has a significant decrease in different kidneys of fish treated with pbcl2 expos
... Show MoreThe experiment was conducted at the University of Duhok's College of Agricultural Sciences. The study used a completely randomized design with four treatment groups, three repetitions, and ten fish per replication in twelve plastic tanks (70 L), each tank receiving proper continuous aeration and cleaning on a daily basis. Positive Control (T2) was higher significantly in each of the Weight Gain, Daily Weight Gain, Relative Growth Rate, Feed Efficiency Ratio, Protein Efficiency Ratio, Fat Efficiency Ratio, Hepatic Index, Kidney Index, Lymphocytes (10^9/dl), Monocytes (10^9/dl), and Granulocytes (10^9/dl). T3 with 25 gm germinated lentil /kg was higher significantly in Intestine Le
... Show MoreThis study was conducted to investigate the impact of adding ajwain seeds to the rations of the growing Ossimi ram lambs on growth performance, nutrient digestibility, rumen liquor fermentation, and blood biochemical parameters. A total of 15 ram lambs, 5-6 months of age, were randomly allocated to the 3 treatments (5 lambs each). Lambs were fed a basal diet and regarded as a control group (T1), while the second (T2) and third (T3) treatments were fed on a basal diet plus 0.2 and 0.4 % of ajwain seeds respectively. At the end of the trial (150 days), three sheep from each treatment were used for a fourteen-day digestibility trial. Feed intake was similar for all groups. The treated groups exhibited higher (P<0
... Show MoreThis study was conducted on 67 local doe goat, aged from 2-5 years old. The overall mean of TMY, DMY and lactation length of 1st and 2nd flocks were (130.541 ± 7.403 kg, 0.716 ± 0.033kg, and 182.432 ± 4.112 day) and (164.043 ± 8.788 kg, 0.832 ± 0.043 kg, 197.027 ± 2.650 day), respectively. The flock, age of doe, doe coat color, month of kidding and type of birth have significant effect on TMY and DMY, doe in 2nd flock production 33.502 and 0.116 kg milk/doe, respectively more than doe in 1st flock. Doe of five years old yielded (181.878 ± 10.171 kg /doe) and (0.892 ± 0.044 kg /doe/day) more milk than young doe. Doe kidding in Januarys were consistently produced high significantly (p≤0.00
... Show MoreIndole mechanism as an interspecies signal particle to adjust various physiological actions, like virulence, antimicrobial and acid resistance. This study was aimed to use partial purified indole extracted from uropathogenic E. coli and study the cytotoxic effect on some cancer cell lines (A375, HepG2, PC3) and comparable with normal cell line (WRL- 68). Serial dilutions of natural and synthetic standard indole were tested (400, 200, 100, 50, 25, 12.5, and 6.25 μg/mL). The results show a low cytotoxic effect for both natural and synthetic indoles in HepG2 and A375 cell lines as well as this degree of cytotoxicity was obviously appeared in normal cell line WRL-68 with no significant differences among them. While, both in
... Show MoreIndole molecules as a signal has an important role in bacterial ecosystem, this study was aimed and conducted on the extraction and partial purification of indole from pathogenic E. coli and study several indole characterizations parameters compared with synthetic standard one. A total of 134 urine specimens and other stool 152 specimens were obtained from Al-Ramadi Hospitals, during the period from November / 2018 to March / 2019.The results show the percentage of isolated E. coli from Urinary tract infection was 100 (74.6%)/134, While 152 (100%)/ 152 are isolated from stool. Primary and secondary screening concluded, there are ten isolates are considered as the best in production of indole. The most producible
... Show MoreThe bacterial ability to form biofilm had been complicated the infection healing process, due to antibiotic resistance. Urinary tract infection is an optimal example of such infections that caused by bacterial biofilms. So the current study aimed to find an alternative method to inhibit the uropathogenic bacteria using nanotechnology. Uropathogenic Escherichia coli (UPEC) was isolated from 110 clinical samples. These bacterial isolated were tested for their ability to form biofilm by two methods: Microtiter plate and congo red agar. Antibiotic susceptibility was detected to determine the multidrug-resistant isolates. An ecofriendly green method was depended for the synthesis of silver nanoparticles (AgNPs) using alcoholi
... Show MoreThis study was aimed to investigate effect of soaking Polianthes tuberosa L. bulbs in Atonic solution and inoculation with Stenotrophomonas maltophilia and Glomus mosseae, and fertilizing with two levels (3% and 4%) of water hyacinths Compost on some vegetative and floral growth characters of the Peral variety of tuberose plants in a Factorial experiment during spring season of 2022 using a randomized completely block design with three replicates. Tuberose bulbs were planted in black plastic bags containing 6 kg of a sandy loam soil. The results of the three-way interaction showed that the treatment of soaking with Atonic, inoculation with the combination of the biofertilizer, and fertilization wi
... Show MoreA field experiment was carried out at Rice Researches Station in Mashkhab, Najaf in 2020 and 2021 seasons to study the effect of cultivars and harvest date on some traits of rice yield. Randomized complete block design was applied within split-plot with four replications. Five cultivars of rice (Amber 33, Yasmin, Dijlah, Amber-albaraka and Furat 1) were distributed in the main plots and five harvest dates (harvest at physiological maturity and harvest after 7, 14, 21 and 28 days of physiological maturity) in the sub-plots. The results showed significant difference among cultivars, and superiority cultivars of Dijlah, Furat 1 and Yasmin in recording the highest yield due to its superiority in number of grains per panicle or
... Show MoreThis study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary co
... Show MoreThis study was aimed to identify efficiency of recurrent selection program to improve maize population. This program was applied to var. Buhooth 106 with var. 5012 Using RGCA with the inbred Zm7 for the production of RSCA. The recurrent selection program was implemented for four consecutive seasons (2020 - 2021) at the Fields Station of the College of Agriculture - University of Anbar. In the spring season 2020, 100 top crosses were obtained from both varieties and progeny. During the fall season 2020, an experiment was carried out to compare the top crosses of each variety RGC
... Show MoreThis study was aimed to investigate effect of some growth traits for two varieties of maize (Zea mays L.) to spraying vitamin B9 (folic acid), vitamin E (tocopherol), and arginine acid. A field experiment was conducted at the Agricultural Research Station, College of Agricultural Engineering Sciences - University of Baghdad, during the fall seasons of 2021 and 2022. Randomized complete block design (RCBD) within split plot arrangement with three replicates was used. The experiment included two factors, the first factor within the main plots represented two varieties of maize (Baghdad 3 and Sarah), while the second factor in the sub-plot represented spraying with vitamin E and B9 at concentrations o
... Show MoreThis study was aimed to examin the effect of CuONPs on both seeds germination, seedling growth and comparing the method of soaking and adding to the culture medium, The research was implemented at PTC. Lab. College of Biotechnology - Al Nahrain University, during 2022 and 2023. The experimental design was factorial within CRD. It was included five experments and ten replicates (4X3). first experment was by using Sodium Hypochlorite (0.0, 1, 2 and 3%) with (5, 10, 15min) duartion time. The second experment was CuONPs (0, 25, 50, 75mg,L‾¹ ) combined with (3,6,9 and 12 day) Time duration, same factors wre examined after soaking seeds with CuONPs which represented the third experment, fourth and fifth experments soaked befor
... Show MoreThis study was aimed to investigate the multi-criteria Analysis based on the geographic information systems (GIS) to determine the most suitable spatial locations for harvesting solar energy in Iraq. Some of the required criteria and conditions, whether technical, environmental, or economic criteria, have been used. In order to identify the suitability of different regions, 6 defined criteria, including solar radiation, distance from power transmission lines, distance from major roads, distance from residential area, elevation, slope, and land use, are identified. The final map of priority of different locations of Iraq for solar energy harvesting is then created by stacking these criteria layers. It has been found that the entir
... Show MoreThis study was aimed to produce a biosurfactant from Lactococcus sp. and study its synergistic effects with some standard antibiotics. Lactococcus sp. HN21 isolate was selected for its highly biosurfactant production and antibacterial activity. It was identified by 16s r RNA as Lactococcus lactis HN21. Optimum conditions for production were studied and it was: Modified M17 media (M17 media with some modifications in its composition) at ph 6.5, for 96 hrs incubation time. The E24% at these conditions was 77%. FTIR and GC-MS results identified the produced biosurfactant as glycolipopeptide with a major fatty acid octadecenoic acid. P. aeruginosa was successfully
... Show MoreMicroRNAs are non-coding gene regulators that may serve as systemic lupus erythematosus biomarkers for diagnosis or prognosis. This study was aimed to identify the single nucleotide polymorphism (rs1140713) of microRNA-126 and its expression by using PCR and qRT-PCR techniques. This research involved 100 womenwith SLE with a mean age of 32.85±9.992 years they were obtained from Baghdad city (medical city, Baghdad Hospital)/Iraq, along with 100 healthy controls with a mean age of 31.68±8.308 years. The mean score on the SLE disease activity index (SLEDAI-2k) was 10.860.±3.275 and a disease duration was 9.00 years, (Erythrocyte sedimentation rate, C-reactive protein, urea, and creatinine w
... Show MoreThis study was aimed to extract, purify and characterize the pancreatic elastase from the Catfish (silurus trioseegus) digestive duct. Different extraction solutions were used to optimize the extraction condition being (Distilled water, 200 mM NaCl, Phosphate buffer (100 mM, pH 8), the latter buffer Tis-HCl (100 mM, pH 8) both containing 0-100 mM NaCl or CaCl2 separately). Tris-HCl buffer containing 100 mM CaCl2 was the best among the rest extraction solutions. The crude extract was precipitated using a 30 % - 55 % of ammonium sulfate saturation ratio. The crude precipitate was dissolved in the same buffer and dialyzed. Then the dialyzed preci
... Show MoreThis study aimed to evaluate biosurfactant production and antibiotic resistance in Pseudomonas bacteria isolated from some agricultural fields to detect the relationship of these isolates traits with some heavy metals resistance. Bacterial isolates were screened for biosurfactant production through blood hemolysis, oil spreading, emulsification activity, and surface tension. The antibiotic sensitivity was determined using disk diffusion method. Then, identification of the selected isolates and subjected to gradient concentrations of heavy metals to determine the minimum
... Show MoreThis study was amied to investigate morphological characteristics of pollen grains and fruits for eleven species belonging to the tribe Brassiceae of cruciferae family, species are: Brassica rapa L , Braasica deflexa Boiss. ,Brassica nigra (L.) brassica juncea(L.), Brassica tournefortii Gouan, Brassica oleracea L., sinapis arvensis L., Hirschfeldia incana(L.) ,Savignya paraflora(Del.) ,Raphanus sativus L. ,Eurca sativa Mill., pollen grains were studied morphologically and all dimentions were recorded. The result of this study showed that most pollen grains are tricolpate ,their size varied between small ,medium
... Show MoreThis study was aimed to evaluate the effect of spraying nano chitosan loaded with NPK fertilizer and nettle leaf and green tea extracts on the growth and productivity of potato for the spring and fall seasons of 2021.It was conducted at private farm in Wasit Governorate, Iraq, as a factorial experiment (5 × 5) within randomized complete block design using three replicates. The first factor included spraying with four concentrations of chitosan nanoparticles loaded with NPK fertilizer 0, 10. 15 and 20% in addition to chemical fertilization treatment, the second factor was spraying nettle leaf extract 25 and 35 gL-1 and green tea extract with 2 and 4 g.L-1, in addition to the control treatment, spraying with
... Show MoreThis experiment was carried out at a private field in the eastern Radwaniyah Baghdad for the fall season 2020/2021 and spring 2021 to study the effects of adding mineral fertilizers, spraying salicylic acid and amino acids on some growth traits and yield of industrial potato plants. 200 kg N h-1 , 100 kg P2O5 h-1, 100 kg K2O h-1 and F2 consist of 275 kg N h-1, 180 kg P2O5 h-1, 200 K2O h-1 and F3 consist of 350 kg N h-1, 360 kg P2O5 h-1, 300 K2O h-1 and salicylic acid in three conc
... Show MoreA field experiment was conducted during fall season 2021-2022 In order to study the effect of soil as an agricultural medium, organic fertilizers (Vermecompost, cow manure) and foliar spraying of silicon and calcium on skin, bark and cambium ring thickness of three beetroot varieties (Red, Dark Red, and Cylindra). Seeds directly sown in the fields of Station A College of Agricultural Engineering Sciences - University of Baghdad - Al-Jadriya campus- Within RCBD a split-plot experiment, where the cultivars were the main unites, organic fertilizer, and foliar spray as the subunits, with three replicates, the number
... Show MoreThe study was conducted us to identify the genetic features of the microsatellite markers CSSM66 and ETH10 and ILSTS34 and their relationship to genetic diversity and productive performance this study was carried out using 100 cows of five breeds presented in Iraq ; Restaki (n=10) , Janoubi (n=10) , Sharabi (n=10) , (local), Holstein (n=50) and male Brahman (n=20) (imported).The results showed the presence of a very high diversity and a highly significant difference in a sample of studied cows in the allele distribution ratios for the three markers, the results showed a significant effect (P≤0.05) superiority of the genotype LY within the ETH10 marker in the least number of inseminatons required for fertilizat
... Show MoreThis study was conducted to identify the genetic diversity of microsatellite markers CSSM66, ETH10 and ILSTS34 using 100 cows of several breeds distributed as follows: 30 local Iraqi cows (equally divided into Restaki, Janoubi and Sharabi) as well as 50 Holstein cows (German) and 20 Brahman bulls after amplification in PCR reaction, 16 alleles of ETH10, CSSM66 and ILSTS34 markers were detected in a sample of studied cattle and they were 7, 6 and 5 alleles, respectively. The total Shannon (I) was 1.5400, and it was found that the average value of Obs_Hom is smaller than the average value of Exp_Het, being 0.2600 and 0.7544, respectively. This indicates the presence of high genetic heterogeneity or diversity among cows. The avera
... Show MoreThe aim of this study was to investigate the effect of adding glycerol at different levels (T1 = control treatment without glycerol, T2 = 75 ml and T3 = 150 ml glycerol) on daily milk yield, milk components and some blood parameters of Holstein cows during the period of 60 days. Milk yield increased significantly (P≤0.05) at 15th day of the experiment, and highly significant (P≤0.01) at 30th and 60th days of the experiment in T3 group. Percentage of milk protein and lactose increased (P≤0.01) in T2 and T3 groups and the percentage of milk fat in the T3 group decreased at day 60 of the experiment. The blood glucose concentration increased (P≤0.01) in T2 and T3 groups at 30 and 60 days of th
... Show MoreThis study was aimed to assess the impact of genetic groups (comprising three distinct lines of Kurdish quails, which are desert, brown, and white), graded levels of L-carnitine and their interaction on the performance of growth traits and blood parameters. For this purpose, the same basic diet was given to 480 Kurdish quail chicks one day old, which was then supplemented for two to seven weeks of age with 0 (control), 150, 350, or 550 mg of L-carnitine per kg of diet. The statistical analysis showed that the lines, L-carnitine supplementation, and their interaction had a significant (p≤0.01) impact on the Kurdish quail's blood parameters, feed intake, feed conversion ratio, body weight, and body weight gain. The desert lines e
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