This study was aimed to determine the active compounds in alcoholic extracts (methanolic 75% and ethanolic 75%) of Pelargonium gravolens leaves and flowers as well as their antibacterial and antioxidant activities. The methanolic extract of dry leaves and fresh flowers (30 g each) gave 4.8650, 2.8015 g respectively. While ethanolic extract for the same parts gave 5.5787, 2.1832g respectively. Spectrophotometric analysis showed that, the flavonoids content in leaves and flowers methanol extract were 20.38, 70.90 μg rutin equivalent/g of extract, respectively, as for ethanol extract those values were 21.89, 71.63 μg rutin equivalent/g of ext
... Show MoreThis study explored the capability of treating real dairy wastewater using the microbial electrochemical system by using Lactobacillus plantarum species as a microorganism. The samples were collected from a local factory for dairy products in Baghdad. Three main parameters were investigated with different levels, for estimating chemical oxygen demand (COD), phosphate (P) and nitrogen (N) removal efficiency. The studied parameters were detention time, the potential difference between electrodes and initial concentration of COD. The respective optimum values for these parameters were 17 days, 400 mV and 17000 mg COD/L respectively. At the optimum values, the optimum COD removal efficiency was 99.4%. Meanwhile,
... Show MoreThis study aims to determine the effect of instant pumpkin soup consumption on the rats' growth, β-carotene levels, Antioxidant Enzyme, Malondialdehyde, and lipid profile. 30 male Wistar rats, 8-10 weeks, were divided equally into six groups. Control-negative (C-) rats were maintained on the AIN-93 diet. Control positive (C+) rats were given AIN-93+beef fat. Instant pumpkin soup groups (IPS 20, 40, 60, and 80) were given AIN-93+beef fat+instant pumpkin soup (20, 40, 60, and 80 g/kg of standard diet, respectively). Blood serum was collected and subjected to lipid profile, MDA (TBARS method), antioxidant analysis (DPPH), endogenous antioxidant enzyme, and β-carotene analysis. Data were analyzed using ANOVA follow
... Show MoreThis study was conducted in poultry farm in college of agricultural engineering Sciences - University of Baghdad - animal production department. In this study 100 laying hens of local Iraqi chickens were used with age 67day old, were placed in individual cages, each cage was numbered from 1 to 100, for the period from 26/10/2021 till 24/7/2022 to detect the different genotypes of LEPR gene, determining the frequency and percentage of the relevant genotypes local Iraqi chickens, , Productive traits were measured from the sexual maturity in to 100 days for each chicken, the blood samples were collected from 100 laying hens at the 38 weeks of age from the brachial vein, the PCR reaction will done us
... Show MoreThis study was carried out to compare the effects of intravaginal FGA sponges to synchronize estrus on reproductive performance of the Karadi and Arabi ewes , 30 for each breed at 2-4 years during the breeding season were used. Ewes from each breed were equally and randomly distributed into three groups. The first group was treated with vaginal sponges saturated with (40 mg) of FGA within a period of 14 days (T1). The second group administrate with the FGA and is injected with a dose of 400 IU of the PMSG hormone (T2). The third group treated with a dose of 0.5 ml/head of PGF2α hormone (T3) on the day of sponge removal, was injected at. The result of the study showed a non-significant increase in the estrous rate, fe
... Show MoreThis study was conducted at the poultry Farm / Animal production department, Collage of Agricultural Engineering Science / University of Baghdad, during the period form 8-3-2014 to 19-4-2014 to evaluation of supplementing different levels of turmeric (curcuma Longa) Powders to Broiler diets on economic indicator and histological measurement of intestine. One hundred and twelve unsexed broiler chicks (Ross 308), one day, 41g its initial body weight. The birds were randomly distributed among Four treatments, each treatment contained 28 birds, with two replicates and each replicate contained 14 birds. The chicks of first treatment (T1) control without supplement, while T2, T3 and T4 treatment were fed a standard diet for broilers in
... Show MoreThis study aims to obtain information on the reproductive biology of these species, especially on sex ratio, fecundity, ovarian development, and breeding season. The study was conducted from August 2022 to July 2023 on every full moon with the highest tide in the estuary of Bengawan Solo River, East Java, Indonesia. The obtained samples were placed in the freezer and identified, and a dissection was performed to observe the gonads. There were 617 crabs with an average sex ratio of 1 male to 1.9 females. Mean fecundity of V. litterata was 45350±3182 egg/individual. In each sampling period, there were always females that were maturing gonads. The ovaries of females during the study were the early developed (stage III), la
... Show MoreBy utilizing Ni-NTA affinity chromatography, the expressed recombinant HSP70 protein was purified, and the SDS-PAGE analysis was used to assess its purity. Purified recombinant HSP70 was used to immunize BALB/c mice, and this had a considerable impact on the anti-HSP70 antibody titers. The results of this study show that the adjuvanted hsp70 injected into the mice had a good immunogenic property. In this study, we measured the effects of HSP70 on catalase stability after exposure it to different range of temperature (40, 45, 50, 55, 60) ˚C, and found that the catalase kept its remaining activity% (99% and 17%) when incubating it in 40 and 60ºC respectively in the present of HSP70, compared to its remaining activity % without HS
... Show MoreThis study was aimed to investigate the micro-morphological characteristics of pollens and seeds for five species of the Brassicaceae family in Iraq belonging to the genus Lepidium, and species of Lepidium sativum L., L.ruderale L. , L.perfoliatum L., L.aucheri Boiss, and L.latifolium L. The characteristics of the pollen grains included the shape of the grain, the length of the equatorial and polar axis, the distance between the two grooves and the groove length , as well as the grain surface configuration. The seeds spherical in shape, while the other species were elongated in shape. As for the seeds, they were studied by the Dissect microscope and
... Show MoreThis study aimed to investigate the impact of nine treatments on tomato plants and their planting location within a randomized complete block design. The treatments included gene silencing of the PL enzyme (RNAi-SlPL) and application of organic pectin and calcium to extend the marketability of tomato fruits and improve their quality. The RNAi-SlPL treatment outperformed others, revealed the lowest weight loss (41.4%) and highest firmness (4.89 kg·cm⁻²) after 36 days of cold storage, along with strong performance after 21 days at room storage (41.31% weight loss, 4.29 kg·cm⁻²). This was followed by CaP1P2 treatment, with 43.7% weight loss and 4.48 kg·cm⁻² firmness after 24 days of cold storage, and 45.87% weight loss w
... Show MoreThis experiment was conducted at the Department of Horticulture and Landscape Engineering, College of Agricultural Engineering Sciences, University of Baghdad, during 2022 season to investigate the effect of spraying with melatonin, potassium sulfate, and organic zinc on the flowering and production stage, of 3-year old Kumquat saplings grafted onto lemon rootstock. The experiment was factorial within Randomized Completely Block Design using three replications. The factors of the experiment were foliar spraying with melatonin, potassium sulfate, and organic zinc. The treatments were applied at two times, spring and autumn. Each of the three factors independently influenced both the fruit characteristics and the concentration of c
... Show MoreThe better approach to control the contamination of food by aflatoxin (AF), is identifying the fungal strains responsible for producing that toxin. To estimate the presence of aflatoxigenic fungi growth and AFs in pistachios, thirty two pistachios samples (8 fresh and 24 salt roasted) were collected from Sulaimani markets (Iraq).Rapid screening method for AFs production were utilized initially based on culture methods, the positive samples confirmed by polymerase chain reaction (PCR) that were applied using DNA extracted from the isolates of A. flavus targeting the genes (afR, aflP, aflD, and aflQ
... Show MoreThis study was conducted to identify resistance sources in the Iraqi wheat cultivars using pathological and molecular approaches. Results revealed that forty-four reeleased wheat cultivars were divided into four groups according to their mean infection percentage to common bunt disease. Resistant group consists of wheat cultivars Farris 1, Ashur, Tamuz 3, AlMadian, AlBaraka, Latiffiya, and Rabia. The mean infection percentage of this group ranged from 0.28-3.98% and significantly was higher than
... Show MoreVegetation diversity patterns are necessary to identify species of plants in the Zawita mountains area, Duhok Governorate, Iraq, to investigate the influence of topographical elements (aspects - north and south, elevations and slope gradients) on diagnosis, density, frequency, and species richness and diversity of species in the Zawita forest understory vegetation. Sampling vegetation was surveyed at (1 * 1 m-2), for 8 quadrats per each zigzag transect. The data were designed with a strip-strip block design, with four replicates for each transect. The results revealed significant differences in frequency, density, and Shannon index due to aspects, elevations, and slope gradient. While other measured traits, species ric
... Show MoreThis study was aimed to investigat integrated system for in vitro growth of paulownia plants by assessing the efficacy of chlorine dioxide (ClO2) as an alternative to autoclave in sterilizing culture medium. Therefore, this study was devised to compare autoclave sterilization at three different times (5, 10, and 15) minutes and three different concentrations of ClO2 (0, 0.4, 0,8, 1) mg/L. The results showed that, compared with (0.4) mg/L concentration, concentrations of (0.8 and 1) mg/L are more effective at sterilizing the culture medium. ClO2 sterilization improved individual single node growth more than autoclave sterilization. Since ClO2
... Show MoreThis study was aimed to examine the anticancer activity of fabricated ZnONPs- L-ASNase conjugate against cancer and normal cell lines. Total of 127 bacterial isolates were taken from clinicalandenvironmentalsamples then identified as E.coli.In this work, 88 isolates were identified as E.coli.L-asparaginase produced from E.coli were screened¸clinical Isolates of E.coli from urine sample have the potential to produce asparaginase that is responsible for cytotoxicity¸the optimal culture conditions for the development of the enzyme L-asparaginase were determined to be 37ºC and pH8. Nitrogen sourceconcentrations at 0.9gm/ml were discovered to maximize enzyme production. L-asparag
... Show MoreThe study area located at Erbil province, Kurdistan, Iraq, seven pedons were elected. Twenty-one soil samples were collected in the study area. Different physiochemical and fertility indices have been used to determine the soils development, despite of generating interpolated maps for them. The results indicated that the low values of clay were found in the less pedon developed and argillic horizon existed in development pedons. Study soils were non-saline, slightly to moderately alkaline, and had relatively high bulk density values. Organic matter is concentrated at the soil surface. Considerable total carbonates are found in studied soils and have irregular distribution manner, as well as have high CEC values. Low C/N ratio due
... Show MoreA field experiment conduct during winter season 2022-2023. This study was aimed to investigate effect number of seeds.m2 to two wheat varieties. The results showed the superiority of V1 in most main stem growth traits (biological yield, number of ears .In square meters, plant height, spike length, spikelets number / ear and chlorophyll content ( 1.87 t. ha, 20.0 seed. m2 , 113.90 cm , 18.40 cm, 26.37 spikelets/ ear and 75.92 (SPAD ) respectively. V1 also excelled in all the traits of branch growth ( biological yield, number of peduncles /m2, plant height, spike length and spikelets number / ear, (16.71 tons .Ha-1, 259.17 ear /m2 ,104.57 cm, 16.83 cm and 23.6
... Show MoreThis study was aimed to investigate combining ability and genetic parameters in summer squash (cucurbita pepo L.). Eight inbred lines of summer squash were crossed in line x tester method in summer season 2020. The parental lines (3 tester and 5 lines) and their off springs (15 hybrids) were planted out in the summer season 2021 at vegetable field, Horticulture Department, College of Agricultural Engineering Science, Duhok University. The experiment lay out using randomized complete block design with three replications. The results indicated that the mean square for genotypes were highly significant effect for tester , line and line x tester in all studied traits. The Tester LBL2 and line PEP1670 were the best comb
... Show MoreThe present study was designed to select a suitable isolate of Lactobacillus sp. in order to use it as a probiotic in a toothpaste for the prevention of dental disease. For this purpose, 30 Lactobacillus isolates were collected from different sources and subjected to a screening program to evaluate their antagonism activities against three oral pathogens: Staphylococcus aureus, E. coli and Streptococcus mutans. Eight Lactobacillus isolates were selected from primary screening according to their ability to inhibit the growth of three indicators which then exposed to a secondary screening to detect their ability to produce high amount of bacteriocin. Based on results, th
... Show MoreThis study was aimed to investigate the role of some Toll-like receptor 5 (TLR5) and E. coli fliC gene polymorphisms with increased risk to recurrent urinary tract infections (rUTI). From 180 specimens (blood and urine) were collected from women of different age, 60 of them serve as control while 120 had rUTI symptoms. After culturing of urine specimens, 43 (35.8%) were identified as E. coli isolates. Four SNPs were identified when amplified and sequenced of TLR5 include rs5744168, rs775385356, rs2072493 and rs5744174. Twelve flagellar antigen serotypes were obtained from fliC sequence of 28 isolates. By using Expasy a
... Show MoreThis research was carried out at University of Baghdad - College of Agricultural Engineering Sciences during the fall season of 2020 and spring season of 2021 in order to evaluate the effect of organic fertilizer and the foliar application of boron on the growth and yield of industrial potatoes (Solanum tuberosum L.). Using factorial experiment (5*4) within Randomized Complete Block Design with three replicates, the organic fertilizer (palm fronds peat) was applied at four levels (0, 12, 24, and 36 ton ha-1) in addition to the treatment of the recommended of chemical fertilizer. The foliar application of Boron was applied at four concentrations which were 0, 100, 150 and 200 mg (H3Bo3
... Show MoreThis study was aimed to test four types of different plants for the purpose of selecting the optimal plant as a source of the amylase enzyme, including wheat, barley, rice, and potatoes. Among these plants, wheat was selected as the best plant source for enzyme extraction because it possessed the highest effectiveness of the enzyme specific activity (3.0 U/mg protein). Furthermore, sodium acetate buffer (0.2 M, pH 6.0) was determined to be the optimal extraction buffer with a 1:5 (w:v) ratio after 75 min, and it provided 68 U/mg protein. The enzyme was concentrated with sucrose before being purified by gel filtration chromatography using Sephadex G-150. The results demonstrated a 1.8-fold increases in final purification folds wit
... Show MoreThe present study was aimed to evaluate the effect of kaolin on copper sulfate toxicity and on some hematological and biochemical performance in Cyprinus carpio L. 240 common carp ranged between 85-95g in weight. A total of 120 fish were used to determine the median lethal concentration LC50 of copper sulfate and 120 fish and randomly distributed into six treatments (two replications for each treatment). The LC50 of copper sulfate was 1.82 mg/l for 48h exposure, then added 0.91mg/l of copper sulfate to all treatment groups except control negative .The experimental treatments included adding kaolin: Control negative (without copper sulfate or kaolin); Control positive 0.91mg/l copper sulfate without
... Show MoreThis study was investigated the relationship between Holstein bulls' fertility with some biomarkers (amino، fatty، and organic acids) and semen characteristics. The study included exploring the relationship among the fertility of Holstein bulls، several biomarkers، and semen characteristics in straws (20-25 straws/bull) preserved for three years. The cows were artificially inseminated (9-10 cows/bull) from semen. Based on the fertility rate، the bulls were divided into three groups، the first bulls of medium fertility (˂65%)، the second of good fertility (65-79%)، and the third of high fertility (>80%). The semen characteristics were evaluated، and the concentration of some amino acids and organic fatty acids was mea
... Show MoreThis study was conducted to evaluate the effects of nisin, potassium sorbate and sodium lactate and their interactions as preservatives in fat oxidation, some quality and microbial characteristics of chilled ground beef meat stored at 4C˚ for 0,4,8 and 12 days. The study included nine different treatments: T1 (control treatment without addition), T2 (nisin at a concentration of 150 mg/kg), T3 (potassium sorbate 0.075 g/kg), T4 (sodium lactate 3%), T5 (nisin 150 mg/kg + potassium sorbate 0.075 g/kg), T6 (nisin 150 mg/kg + sodium lactate 3%), T7 (Sodium Lactate 3% + Potassium Sorbate 0.075 g/kg), T8 (Nisin 150 mg/kg + Sodium Lactate 3% + Potassium Sorbate 0.075 g/kg) and T9 (Addition of Butylated hydroxyanisole(BHA 0.01%)). All tr
... Show MoreThe objective of the current study was to investigate the influence of adding resveratrol (Res) to a milk-based extender on some post-thawed semen quality of Iraqi buffalo bulls for different preservation periods (cooling at 5˚C, 48 h, 1, and 2 months post-cryopreservation; PC). Four bulls, 3.5 to 4 years old, were used from 11th November 2020 to 1st June 2021. Semen samples were collected, pooled, and divided into four groups using different Res concentrations (0, 100, 150, and 200 μM) added to the milk extender. The Res-150 μM group exhibited higher (P≤0.01) plasma membrane, acrosome integrity percentages, and total antioxidants concentration (TAC) compared to the other groups. Sperm abnormalities and DNA damage percentage
... Show MoreNickel is an essential nutrient for plant growth with low concentrations, its excessive amounts in soil above threshold values could be cause in toxicity. The main objectives of the present research were to determine the effects of nickel nanoparticles foliar spray with 20, 40 and 70 nm diameter on the physiological characters and anatomical aspects of Phaseolus vulgaris L. plants. Lowest reduction significantly (P < 0.01) in root and shoot biomass was recorded due to in 70 nm; the measurements 0.08 and 0.05 g per plant and highest root: shoot; 0.65 as compared with control treatment. As well as the lowest conserved water content; 40% was observe
... Show MoreThe present study was aimed to test the antagonistic activity of endophytic fungi isolated from Aloe vera leaves against a number of plant-pathogenic fungi. The results showed that the highest antagonistic activity was exerted by Penicillium chermesinum against the pathogenic fungus Rhizoctonia solani, with an inhibition rate of 78.57%. Also, Talaromyces verruculosus and P. chermesinum showed their highest antagonistic activities against pathogenic fungus Fusarium 1 with inhibition rates of 61.79% and 61.53%, respectively, while T. verruculosus showed an inhibition rate of 62.91% against the pathogenic fun
... Show MoreThe experiment was conducted using Potato( Solanum tuberosum L.) at the eastern Radwaniyah at private field during fall season 2020/2021 and spring 2021 to study the effect of nitrogen levels to 350, 275, 200 kg N h-1 ( N1, N2, N3) and phosphorous to 100, 180, 360 kg P2O5 h-1 ( P1, P2, P3) and potassium to 100, 200, 300 kg K2O h-1 ( K1, K2, K3) to vegetative growth and yield of industrial potato, The seeds of the hybrid potato Sinora, Class A, were planted in the fall season on 15/9/2020 and Elite in the spri
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