The present study was carried out to investigate immunopathological effect of foot and mouth disease vaccine against nosocomial infections, to achieve this goal different samples obtained from urine of hospitalized patient, the isolated bacteria were identified by cultural examination, biochemical tests PCR. Twenty-four mice were divided equally into three groups. The 1st group 8 mice were immunized I/M with 0.1 ml of FMD vaccine, two dose with 14 days intervals, 2nd group 8 mice was inoculated I/P with 0.5 from 3x108 CFU/ml while 3rd group was inoculated 0.2 ml of phosphate buffer saline and served as control negative. At
... Show MoreExperiment of half diallel cross among eight maize inbred lines was carried out during spring 2021.The varietal traits for F1 cross and parents with local hybrid were conducted during autumn 2021 Field of College of Agricultural Engineering Sciences , University of Duhok, using Randomized Complete Block Design with three replications .The results were revealed that the analysis of variance for genotypes and general and specific combining ability were significant effect on studied traits , also the ratio of degree of dominance is less then one for all traits . The cross 7x8 gave the heighest value ( 219.35g) for grain yield
... Show MoreAntimicrobial properties of Syzygium aromaticum were investigated and phytochemical active groups were done, clove oil contain a unique active compound known as eugenol was extracted and isolated to be tested. Four different pathogenic bacterial strains (Staphylococcus auras, Listeria monocyogenes, Salmonella typhimurium and Escherichia coli) were obtained and isolated from contaminated food, three concentrations for the plant (eugenol extract) were prepared to be a treatments, T1, T2 and T3 with percentage of 100%, 50%, 25% respectively. Antimicrobial activity was measured by using disc diffusion method and the best treatment was T1, which tested for eugenol qualitative and quantitati
... Show MoreTwo hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which i
... Show MoreThe present investigation was carried out to compare the impact of cinnamon and clove powders and their oil additives as anti-stressors on quails under heat stress which is one of the riskiest environmental conditions that influence poultry all over the world in general and Iraq in particular. For this purpose, 420 quails (5 weeks old) were chosen and randomly grouped into seven treatments with different treatments (T0: control (standard diet), T1: 2 g clove powder/kg diet, T2: 1 ml clove oil/kg diet, T3: 2 g cinnamon powder, T4: 1 ml cinnamon oil/kg diet, T5: 1 g clove powder+1 g cinnamon powder/kg diet, T6: 0.5 ml clove oil+0.5 ml cinnamon oil/kg diet) added to standard diet. The quails were fed with these diets for 17 weeks. T
... Show MoreFrom 2017 to 2019, 51 A. rabiei isolates were isolated from 259 chickpea fields across IKR. On different media, 32 isolates showed significant differences in morphological characteristics. The isolates were divided into six groups based on colony color, five groups on mycelium color and three groups on pycnidia color. CSMDA was the best media for mycelial growth. AS-28 significantly surpassed all other isolates in colony diameter despite media type. A. rabiei growth varied between 15-35°C, the maximum growth occured at 25°C and ceased at 35°C. The mean conidia and pycnedia dimensions in isolates AS-19 and AS-9 ranged from 20.0*7.5μm and 70.8*47.9μm
... Show MoreThe objective of this study was to investigate association between FecGH-GDF-9 gene polymorphism and litter size in Hamdani fat-tailed sheep. The genomic DNA was extracted from eighty-seven blood samples of Hamdani ewes. The FecGH-GDF-9 locus was detected by PCRs, and identified DNA genotyped by DNA sequencing. The evaluate several parameters included fertility (%), conception (%), letter size at birth, twining rate (%), triple rate (%), barrenness (%) and productivity (%) were measured and arrived 91.95, 95.40, 1.825, 150, 11.25, 8.05 and 159.77, respectively. The blast tree view results in NCBI blast show Hamdani sheep is more closely to Norway white face sheep, Han sheep and Pelibuey Sheep breeds which ha
... Show MoreLaboratory experiment was carried out in order to study the adsorption of Fe as FeEDDHA as related to some soil properties in six calcareous soils from the middle of Iraq. Langmuir, Freundlich and Temkin equations were used for describing Fe adsorption. The best equation was chosen according to highest value of r, r2 and t and least value of Standard error of estimate. The results showed that Freunglich equation was more efficient for describing Fe sorption compared with other physicochemical equations used, depending on highest values of, r (0.985), r2 (0.970) and t ( 20.01) and lower standard error SE.e (0.186). Maximum adsorption capacity
... Show MoreA field experiment was conducted at the experimental field, Dept. of Field Crops, Coll. of Agricultural Engineering Sciences, University of Baghdad, Al-Jadiriya, Iraq, during the winter seasons of 2018-2019 and 2019-2020, to study the effect of foliar application of moringa leaves extract at different stages of growth and its reflection on yield and quality of bread wheat (Triticum aestivum L.) var. Rasheed by determining best concentration and the spraying stage to raise the efficiency of production. A split plot arrangements was used according to RCBD design with three replicates, where the main plots contained three concentrations of moringa leaves extract (1/10, 1/20
... Show MoreThis study was carried out to detect the Dematiceous hyphomycetes fungi that associated with Zinnia seeds and to test their pathogenicity and effect on seed germination and foliage. Results of isolation and identification showed the existence of 8 species of these fungi associated with Zinnia seeds. Alternaria alternata was presented in 81.8% of seed samples with highly rate of frequency 77% in sample No.1. The preliminary test of the pathogenicity by cabbage seeds which included three separated experiments, showed that the 34 tested isolates caused a significant reduction in the rate of cabbage seeds germination except B18 isolate (Phoma eupyrena). Isolates B39 and H7 (Alternaria alternata ) were highly
... Show MoreThis study was carried out in Al-Qadisiya Governorate during the period from December , 2012 to March , 2013. Involving five regions with different geographical locations (Diwania city center , Saniya District , Shafeia District, Daghara District and Al-Hamza District ) to determine the residues of nickel , arsenic and mercury in raw milk of cows , sheep and goats . A total of 150 raw milk samples were collected from 150 animals belong to the three species, involved 50 samples for cows, 50 samples for sheep and 50 samples for goats (10 samples / region) selected randomly. Atomic absorption spectrophotometry was used to determine the concentration of nickel (NI) , arsenic (AS) and mercury (Hg) . The results showed that a significant
... Show MoreThe study sample included 590 farmers in Wasit province, has been taking samples of soils that the first two levels of the samples 0-15 cm, and second 15-30 cm, from different agricultural people in the province, was the level of salinity of the soil analysis, in the laboratories of the Faculty of agricultural techniques / Musayyib, SPSS software to analyze the function and use the influence of the level of soil salinity on the productivity of wheat crop, and traversed by testing the function of class I and II, and turned out to be an inverse relationship which determine the level of critical soil salinity b 12.13 Desiminz / m, a level which even then the average revenue dunum with average costs, also identified the l
... Show MoreThe present study was conducted to evaluate wound induced in rabbit skin by using alcohols and aqueous extracts of pomegranate seeds. Total flavonoids concentration were estimated using high performance liquid chromatography (HPLC). The highest flavonoids concentration were recorded in ethanol extract. Acute inflammation was induced in laboratory animals (rabbits) by injecting 0.1mL of 2.5% formalin inside the wound incision. Aqueous and ethanol extracts of pomegranate seeds an anti-inflammatory and healing effect compared with the Vaseline
... Show MoreA field trial was carried out at the Rice Research Station in Mushkhab ( Najaf/ south of Iraq)State Broad for Agricultural Researches, during the Summer of 2013 in Clay Loam soil. The experiment was laid in split plot with two replications, to test the drought tolerance of different local and foreign varieties of rice .Rice varieties used were 6 varieties of aerobic rice from the International Rice Research Institute(IRRI) and local cultivars Anber33 and Jasmine. Two methods of irrigation ,flood irrigation, and 6 irrigation levels at "-10,-33,-50,-100 KPa" were used in this investigation .Studied parameters were: plant height ,spike length, flag leaf area, % of infertility , weight of 1000 grains and grain yield. Resu
... Show MoreThis study was aimed to know the efficiency of partially purified L- asparaginase produced from local isolate from Erwinia spp. to reduce the percentage of acrylamide formed in Biscuit. Four types of biscuit from wheat flour were prepared (T1, T2, T3, T4),and T1 as control. High performance liquid chromatography technique was used to estimate acrylamide ratio in biscuit , Effect of enzyme addition on flour chemical and rheological properties was studied, also dough behavior ,gluten percentage, water absorption and amylase enzyme activity was estimated. The results revealed that the addition of experimental asparaginase ( specific activity 20.5 unite mg-1 ) with 1% of flour weight
... Show MoreThis research was conducted to study the effect of food treatment leaves of Malva sylvestris with ethanol ,ethyl acetate, hexane and oil extract of black pepper fruits Piper nigrum on some biological aspects of cotton leaf worm Spodoptera littoralis by using the concentrations of 1.25, 2.5 and 5% of each extract, The results of feeding treatments of second larvae instar showed an increase in mortality percentages which reached 100% at the all extracts when using 5% concentrate. used in the test, while the highest mortality reached to 26.7% when using ethanol extract at 5% concentr
... Show MoreThis study was conducted on the 22 taxa of the genus Trifolium L. which grow naturally as wild plants in all geographical districts in Iraq. A comparative study of the leaf petioles was anatomically examined by paraffin method. Petiole cross-section outline shapes have divided the genus species into fourteen groups and sub-groups. According to the microscopical characteristics the cortex of petioles showed to contain layers of various tissues, the collenchyma tissue observed sub-epidermally in some species, and cortexes of the others were devoid of collenchyma. The sclerenchyma strands were noted in three patterns; above vascular bundles, above and beneath vascular bundles, and the sclerenchyma strands completely surroun
... Show MoreThis study was aimed to evaluate the antimicrobial activity of gold nanoparticles that was synthesized by biological method using Aloe Vera extract. The Surface morphology of the synthesized gold nanoparticles was confirmed by Atomic force microscope (AFM) while the nature of functional groups present in gold nanoparticles was determined by FT-IR analysis. The antibacterial activity of gold nanoparticle was tested against multidrug resistance (MDR)pseudomonas aeruginosa, the results showed a significant effect against MDR isolates. Gold nanoparticle was loaded with ceftazidium antibiotic in order to improve the antibacterial activity and drug delivery efficiency. The synergistic effects of biosynthesize gold loa
... Show MoreThis experiment was carried out at the experimental farm of Field Crop Department, College of Agriculture, university of Baghdad, during two spring seasons of 2012 and 2013 to study the response of sunflower cultivar Akmar to irrigation methods, magnetized water technology on growth characteristics, yield and water use efficiency. The experiment was laid out as a split plot in randomized complete block design with three replications. Four irrigation methods were used as main plots, [farrow irrigation (I1), unfixed alternate furrow irrigation (I2), fixed alternate furrow irrigation (I3) and basin irrigation (I4)], while four levels of magnetized water (0
... Show MoreThis study was aimed to investigate the Open Reading Frame-112 (ORF-112) gene, which encoded for a hypothetical 218 amino acids protein in Streptomyces bacteria. A complete ORF-112 gene was synthesized, with addition of a 6xHis-Tag at the N-terminal location. The synthesized DNA nucleotides were sub-cloned into bacterial expression plasmid pBAT4. The pBAT4-ORF-112 plasmid transformed in bacterial cells BL21(De3)pLysS, intended for protein over expression, induced by isopropyl β- d-1-thiogalactopyranoside (IPTG). The IMAC affinity chromatography technique was deployed for protein purifications. Highly-purified fractions of ORF-112 were achieved by using affinity Ni++-columns. The purified ORF-
... Show MoreThis experiment was performed to find out the effect of peppermint on the performance, gut morphology and immune organs of coccidiosis challenged broilers. A feeding trail was conducted on 320-day-old Ross 308 chicks allocated to 32 floor pens in a 2x4 factorial arrangement. The studied factors were: challenge (Negative or Positive) and feed additive (Control, Peppermint and). On day 8 of age, birds in challenged groups were orally inoculated with Eimeria tenella. On day 35 Eimeria decreased weight (P <.0001) gain and deteriorated feed conversion ratio (P < 0.05). On 24 and 35 days of age, broilers given salinomycin and p
... Show MoreLemongrass (Cymbopogon citratus) plant belongs to the Gramineae family. Lemongrass leaves essential oils were extracted by Clevenger method, antibacterial, MIC and MBC were evaluated against some gram positive and gram negative bacteria. Bacillus cereus, Staphylococcus aureus, and micrococcus spp., recorded high sensitivity to essential oil with inhibition zone reached (40, 32, and 28) mm respectively. While Pseudomonas spp., Salmonella typhimurium, and Escherichia coli recorded (20, 20, and 22) mm respectively. MIC and MBC values reached (3, 6.5) % respectively for gram-positive bac
... Show MoreA field experiment was carried out at the private vineyard located at Bara-Bhar village, Duhok Governorate, Kurdistan region, Iraq, during the seasons of 2016 and 2017 to investigate the effect of three concentrations of micronutrients (0, 50 and 100 mg.L-1) and determine the optimum bud loads per vine for "Mirane" grapevines. Twelve years old uniform vines were chosen and pruned to four different levels of buds load, namely 36, 44, 48 and 64 buds.vine-1. The factorial experiment within randomized complete block design, with three replication was used. The results showed that the application of micronutrients especially at high concentration significantly increased leaf area, total chlorophyll content, numbe
... Show MoreThis study was conducted to estimate the effect of adding different levels of turmeric powder and curcumin on broiler performance, carcass traits, and immunity response and jejunum histology. A total of 750 Ross day old chicks obtained from a local hatchery and distributed into two rearing conditions (normal and heat stress condition) with five treatments 3 replicate for each one had (25 chicks). Dietary supplements of turmeric powder and curcumin significantly (P ≤ 0.05) improved performance under normal and heat stress conditions. Moreover, treatments significantly (P ≤ 0.05) increased the total titter antibody against Newcastle and infectious bursal disease compared to control. Concerning histology of jejunum treatments si
... Show MoreThis study aims to estimate critical concentrations of cadmium (Cd) and lead (Pb) in the soil which negatively affect growth of Eucalyptus camaldulensis Dehnh. seedlings, and to estimate some phytoextraction parameters for heavy metals (HM) from the soil to evaluate efficiency of seedlings in their potential use in phytotechnology to improve the environment with phytoremediation. Eucalyptus seedlings were treated with Cd concentrations 0, 25, 55, 85, and 110 mg kg-1 dry soil as CdCl2, and Pb concentrations 0, 125, 250, 450, and 550 mg kg-1 dry soil as PbCl2, and the experiment was designed using the completely randomized design (CRD) as a two-factor factorial experiments and the data we
... Show MoreIn the current study, four types of plants commonly used namely Soybean, chickpea, bean, pea were obtained and screened for urease activity, among this plants, chickpea was chosen with maximum enzymatic activity, and it had the highest productivity of urease enzyme (1243 U/mg protein). Also sodium acetate buffer (0.2 M, pH 5.0) was chosen as a best extraction buffer with specific activity 1460 U/mg protein. The optimum extraction ratio represented by 1:8 (w:v) after 15 min, it was given 1988 U/mg protein. As well as four types of plants include garlic, red onion, green onion and cabbage were used to select the optimum plant material that inhibited urease enzyme. Cabbage was chosen, it had the highest inhibition activity of the en
... Show MoreSome fertilizers practices could be used to improve Baraka seed Nigella sativa L. as a medicinal crop like organic farmyard manure which efficiently applied to enhance growth and yield. Thus, a field experiment was conducted out at Research Station in Tikrit-Iraq during 2012/2013 season to estimate the impact of bat guano (0, 75, 125 and 175 kg.ha-1), seaweed extract as kelpak (0, 1, 2 and 3ml.l-1) on some physical and chemical essential oil components. Two factors were arranged in factorial experiment in randomized complete block design with three replicates. Thus, results revealed that application of bat guano at 125 kg.ha-1 was superior in enhancement of essential oil and its some physical and
... Show MoreThis study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuate
... Show MoreThis study was carried out to isolate, identified and study two types of Iraqi fresh water algae Scenedesmus sp. and Oscellatoria sp also determine some of their active compounds that play an important role as antioxidants and antimicrobial againsts some bacteria. Three types of bacteria Salmonella sp, Escherichia coli and Staphylococcus sp that cause spoilage for food were tested. Antimicrobial activity of ethanolic extracts for both algae were examined toward tested bacteria. Four treatments were prepared which were [S.Ex(T1) ,O.Ex(T2), S.Ex: Of*(T3), 1:1 O.Ex: Of*(T4) 1:1],and (C) as control treatment with the antibiotic only (Oflaxacin). Disk diffusion method was used t
... Show MoreThis study was carried out in order to identify and mapping the temporal changes for land covers in Baghdad province using Remote Sensing and GIS. Three images were used of land sate taken in 1976, 2000 and 2014 the study area. Suppervised classification and SAVI Index were used to identify land cover classes dominated in the study area. The results of supper classification indicated the presence of five land cover classes including water bodies, bare land, urban, low dense vegetation, and dense vegetation classes. There were four classes of land cover when was used SAVI index: water, no vegetation (bare land and urban), low dense vegetation, and dense vegetation. The results showed that Remote Sensing is a very active and useful
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