Baker’s yeast (Saccharomyces cerevisiae) has been genetically engineered to
ferment the pentose sugar xylose present in lignocellulosic biomass. One of the
reactions controlling the rate of xylose utilization is catalyzed by xylose reductase
(XR).The current study describes xylose reductase from Spathasporapassalidarum
with NADH preference. According to JGI site the gene coding for this enzyme
contains 954 nucleotides and it consists of 317 amino acids. The restriction sites for
the enzymes SacII and NotI located on the 5P
´
Ptermini for both the forward and
reverse specific primers were designed using Lasergen 9.0 program. The genomic
DNA was isolated and purified from S .passalidarum. Polymerase chain r
An extracellular β-galactosidase from the thermophilic fungus Rhizomucor
Pusillus IB8 has been purified via several steps included precipitation by ammonium
sulphate at 80 % saturation, DEAE- Cellulose Ion exchange chromatography and gel filteration on sepharose CL-6B column. The Final purification folds and the yield of the enzyme were 42.5 and 24.8 % respectively. The purified β-galactosidase has an optimum pH for its activity between 4.5 to 5, while the optimum pH for enzyme stability was between 5 to 5.5. Futhermore, it was found that the optimum temperature for its activity was 60 C°. The purified enzyme retained approximatly 98% of its original activity when incubated at 60 C° for 60 min. However, 25 % of its activity was
One hundred and six S. aureus were isolated from 250 Nasal swabs of
Healthcare workers and patients at Al- Kadhamia teaching Hospital and Al-
Numan hospital, Baghdad, Iraq. The study was undertaken over a period of
ten months between August 2011 and June 2012. S. aureus isolates were
diagnosed based on phenotypic traits and biochemical tests. Antibiotics
sensitivity to 11 antibiotics, revealed that S.aureus is totally resistant to
Pencillin G (100%), highly resistant to Cefoxitin (alternative to Methicillin)
(94.3%) While there are varied resistance percentage for the rest of
antibiotics: Erythromycin (37.7%), Tetracycline (34.9%), Gentamicin
(29.3%), Trimethoprim/sulfamethoxazole (50%), Ciprofloxacin (29.2%),<
This research includes new series of polymers were synthesized starting from polymerization of poly maleic anhydride (3), which were mono esterfication with absolute methanol to give polymer (4 ), then the acid side chlorination with thionyl chloride to give polymer (5) , which was subjected to esterfication with two different protected sugar moiety (1 and 2 ) to afford modified polymers (6 and 7). Furthermore the hydrazide polymers ( 8 and 9) were prepared through addition of hydrazine hydrate to polymers ( 6 and 7 ), which upon condensation with different free mono saccharides to give the target polymers( 10-13) respectively. The prepared polymers identified by physical properties and spectral methods (FT-IR ,1H-NMR,13C-NMR) .
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The ground state proton, neutron, and matter density distributions and corresponding root-mean-square (rms) of P19PC exotic nucleus are studied in terms of two-frequency shell model (TFSM) approach. The single-particle wave functions of harmonic-oscillator (HO) potential are used with two different oscillator parameters bRcoreR and bRhaloR. According to this model, the core nucleons of P18PC nucleus are assumed to move in the model space of spsdpf. The shell model calculations are carried out for core nucleons with w)20(+ truncations using the realistic WBP
interaction. The outer (halo) neutron in P
19
PC is assumed to move in the pure 2sR1/2R-
orbit. The halo structure in P
19
PC is confirmed with 2sR1/2R-dominant c
The Polarographic study of the interaction between ascorbic acid and Ni+2 was carried out at dropping mercury electrode [DME] . This study included the determination of the kinetic parameters (kfh ,n) and thermodynamic parameters such as enthalpy change (H), free energy change (G) and entropy change (S) of Ni+2 complexes with ascorbic acid in 0.1 M KCl solution over the temperature rang of (294-309)K. The electrode processes were irreversible and diffusion controlled.
The present work included morphological, anatomical, and palynological
characters for the new species Acaalypha australis L. specimens, which belong to
the family Euphorbiaceae. The species recorded in the study for the first time in
Iraq. The plants of this species are annual herbs with green, striated or sub –
polygonal stem, and branched near bases, Leaves are simple spirally alternate and
lanceolate in shape. Flowers are unisexual, arranged in the axial of distinct leafy and
cordate bracts, female flower arranged at the bracts bases and each flower with
trileafed perianth and superior ovary with trilobed stylar stigma which has dense and
coiled stigmatic hairs. Male flowers are arranged as a mixed verticella
The calcination treatments and a binder of poly acrylic acid PAA (1wt%)
effects on kaolinite particles were investigated through dielectric properties at
1MHz ,quantitative analysis of X-ray diffraction and microstructure. The calcinated
samples at 850°C/3hr and fired at 1350°C/2hr were revealed decrease in broadening
(Full Width at half maximum) FWHM and increase of dielectric constant.
Fifteen blood samples were collected from healthy males and females (6 males &
9 females), average age (21-34 years) in heparinized sterile tubes. The extracellular
protease was extracted from a clinical isolate of Serratia marcescens that was
isolated from a patient suffering from urinary tract infection taken from the Central
Health Laboratory. The extracted protease was purified partial by two steps,
precipitation with 30-55% saturation of ammonium sulfate following with dialysis
and ion exchange chromatography DEAE-cellulose. The protease concentration was
0.15 mg/ml. Two concentration 0.258g/ml and 0.58/ml of protease were prepared
and applied in current study. Lymphocyte transformation test using whole b
Seasonally variations in Physio-chemical parameters of Al-Auda Marsh were investigated from November 2012 to July 2013. In this research examine pH, turbidity, EC, DO, BOD5 and Ca+2, Mg+2, NO2 , NO3 , PO4 , SiO3 ions. Six sites were randomly located and water samples. pH values varied from (7.3 ±0.0) in site 4 during autumn to (8.5±0.5) in site 6 during winter, EC values ranged from (2020±186) μ.s/cm in site 5 during autumn to (6390±875) μ.s/cm in site 6 during summer, mean turbidity data were found to vary from (6.5 ±1.8) NTU in site 4 in summer to (31.0 ±1.6) NTU in site 3 in autumn. DO highest mean value (10.9 ± 0.6) was for site 6 in autumn and the lowest value (6.5±0.9) mg/l for site3 in spring. In case of BOD5 highest and
... Show MoreThe object of this study was to evaluate the efficiency of safranin O (SFO), dye removal with application of Thuja orientalis as a low-cost biosorbent. The biosorption equilibrium level was determined as a function of pH, adsorbent dose, contact time and temperature. Surface area and pore size distribution were measured for the adsorbent. Thuja has a good removal effeciency for SFO dye. The adsorption kinetics data were best fit for the pseudo-second order kinetic (the regression coefficient = 0.999). The experimental equilibrium adsorption data are tested for the Langmuir, freundlich,Temkin and Dubinin-Radushkevich isotherm modles. From the values of the regression coefficient the results indicate the following order to fit the isotherm
... Show MoreThe ground state charge, neutron and matter densities for two-neutron halo nuclei P
12
PBe
and P
14
PBe are calculated within a two- frequency shell model approach. In the description of
the halo nuclei it is important to take into account a model space for P
10
PBe and P
12
PBe different
from the two halo neutrons which have to be treated separately in order to explain their
properties. The structures of the halo P
12
PBe and P
14
PBe nuclei show that the dominant
configurations when the two halo neutrons distributed over the 1d shell orbits. Elastic
Coulomb scattering form factors of these two exotic nuclei are also studied through the
combination of the density distributions of
In this study, the magic nuclei is divided into two groups, one of them is light group and the other is middle group, it was calculated shell corrections for all nuclei, and also it was concluded the relationship between cross sections for nuclear reactions ()α,n and the mass number (A) for all nuclei to incident neutrons (14.5 MeV). We found empirical equations to asymmetry parameter (N-Z)/A as function of mass number and for that two groups: for A=38 to A=40 light nuclei.()0534.10263.0+−=−AAZN for A=50 to A=89 middle nuclei. ()408.00151.00001.02−+=−AAAZN for A=90 to A=144 middle nuclei. ()0711.10221
The study of thallium intoxication on 120 broiler chicken by using different
doses of thallium, 0.2; .5 and 1.0 mg/kg. B.Wt. it was observed that the clinical sign,
bloody diarrhea, appeared in the last ten days of the experiment. In second and third
group the pathologic changes was hemorrhages and edema of skeletal muscles and
brain. Petechial hemorrhage in the testis, ovary and thyroid gland. The
histopathological changes were comprised of hyperplasia, cellular, infiltration in the
thyroid gland, narrow cortex of the ovary, in the brain there were remarkable edema
with perivascular cuffing and loss of purkinge cells in the cerebellum. The results
suggest that thallium was accumulative.
The present work aimed to examine the nature and degree of the cross-correlations among three different ionospheric indices: these are Optimum Working Frequency (OWF), Highest Probable Frequency (HPF), and Best Usable Frequency (BUF). VOCAP and ASASPS models were adopted to determine the datasets of the selected ionospheric indices. The determination was made for different transceiver stations that provide certain HF connection links during the minimum and maximum years of solar cycle 24, 2009 and 2014, respectively. Matlab program was implemented to produce the geodesic parameters for the selected transceiver stations. The determination was made for different path lengths (500, 1000, 1500, and 2000) Km and bearings (0o
... Show MoreA simple, rapid and sensitive spectrophotometric methods for determination of
trace amounts of (OXC) as (OXC-Au(III)) complex in octane as solvent for
extraction the complex. The green-blue soluble product give maximum absorption at
705 nm, Beer’s law is obeyed over the concentration range of (5-35) μg.ml-1 with
(molar absorptivity = 1.606×104 L.mol-1.cm-1), (r = 0.9981), (D.L = 0.0864 μg.ml-1),
(%RSD = 2.020), with UV-Vis method.
When using indirect FAAS a linear range of (2-16) μg.ml-1, (r = 0.9992), (%RSD =
2.667), (D.L = 0.0526 μg.ml-1) with (ε = 6.02×105 L.mol-1cm-1).
The optimum conditions for colour development are described. The proposed
methods have been successfully applied
The current study includes the preparation of CuxAl0.3-xNi0.7Fe2O4 ferrite nanoparticles where: x = 0, 0.05, 0.1, 0.15, 0.2, 0.25, and 0.3 using the auto combustion method (sol-gel). The citric acid was used as fuel for auto combustion. The X-ray diffraction (XRD) test showed an increase in the crystal lattice constant, which is of face-centered cubic (FCC) structure. Field Emission Scanning Electron Microscopy (FE-SEM) showed that the compound has apparent porosity; its grains are spherical or semi-spherical and within the nanoscale. The Energy-Dispersive X-ray spectroscopy (EDX) proved the presence of all the elements involved in the preparation of the
... Show MoreCholesterol oxidase (EC:1.1.3.6) is industrially and commercially important for
the application in bioconversions for clinical determination of total or free serum
cholesterol, and in food industry, for this reason this study was aimed to isolate
cholesterol oxidase producing Pseudomonas aeruginosa from different local
sources, by taking 55 swap samples from different serological samples includes ears,
urine, burns, wounds and sputum, in addition to another 35 environmental samples
collected from soil and water from different locations in Baghdad governorate. From
these samples 115 bacterial isolate were obtained, and among them only 60 isolate
were identified as P. aeruginosa according to the resu
Let M be ,-ring and X be ,M-module, Bresar and Vukman studied orthogonal
derivations on semiprime rings. Ashraf and Jamal defined the orthogonal derivations
on -rings M. This research defines and studies the concepts of orthogonal
derivation and orthogonal generalized derivations on ,M -Module X and introduces
the relation between the products of generalized derivations and orthogonality on
,M -module.
This study has been achieved to detect the bacterial contamination of red local
and imported meat (Saudi, Australian and Indian). 12 Kg of meat were bought from
six different places of Baghdad (Aldura, Almahmodia, ,Algehad, Alsader city,
Alkhademia, Albyiaa). Meat extraction samples were cultured in different diagnostic
and nutrient cultures to detect bacterial contamination which represented mainly of
Coliform, Staphylococcus aureus and Salmonella bacteria. The results of the
imported meat showed high level in the count of those bacteria in addition to another group (not diagnosed) mentioned as “different species “of bacteria. All of them reached the maximum level of permitted range which specified by “Central Or
In this paper, the conditions of persistence of a mathematical model, consists from
a predator interacting with stage structured prey are established. The occurrence of
local bifurcation and Hopf bifurcation are investigated. Finally, in order to confirm
our obtained analytical results, numerical simulations have been done for a
hypothetical set of parameter values .
Let R be an associative ring with identity. An R-module M is called generalized
amply cofinitely supplemented module if every cofinite submodule of M has an
ample generalized supplement in M. In this paper we proved some new results about
this conc- ept.
The k-out-of-n:G (or k/n:G) system structure is a very popular of redundancy in
fault-tolerant systems, with wide applications in so many fields. This paper presents
two states of multi-state k/n:G systems. The first part, we present the definition that
introduced by Al-Neweihi et al [1], where the values are the same with respect
to all system states and we show that there exists an alternative equivalent definition
to Al-Neweihi's definition. In the second part of this paper we give more general
definition proposed by Huang et al [2], where it allows different values with
respect to different system states and we provide there exists an equivalent definition
to Huang's definition when the values are increasing.
The aim of this paper is to analyses steady state three-dimensional magnetohydrodynamic(MHD) flow of fluid injected uniformly into the vertical channel with porous wall through one side of the channel. The equations which were used to describe the flow are the momentum and energy equations, these equations were written to get their non-dimensional form. It is found that these equations are controlled by many dimensionless parameter, such as Hartmann number M, Reynolds number Re and Peclet number Pe. The homotopy analysis method(HAM) is employed to obtain a analytical solutions for velocity and heat transfer fields. The effect of each dimensionless parameters upon the normal and tangential velocity, pressure and temperature distributions
... Show MoreBased on the density functional theory (DFT) , the stability of molecular complexes has been predicted according to hard-soft acid base (HSAB) theory. Relative stability of products and reactivity of soft base sulfide derivatives with halogens (Iodine , Bromine , Chlorine) as soft acid was studied to determine the relative ability of these reactants causing the reaction to be more spontaneous.
DFT at the levels of B3LYP/3-21G and B3LYP/3-21G (d) was used to study HOMO LUMO energy gaps , bonds length and total energy to calculate the softness sequence of each type of acid or base mentioned in this work. All cases studied prove that iodine can be considered as the most softness acid and ethyl methyl sulfide≈ dimethyl sulfide the most
DFT (3-21G, 6-31G and 6-311G/ B3LYP) and Semi-empirical PM3 methods were applied for calculating the vibration frequencies and absorption intensities for normal coordinates (3N-6) of the Tri-rings layer (6,0) Zigzag single wall carbon nanotube (SWCNT) at their equilibrium geometries which was found to have D6h symmetry point group with C-C bond alternation in all tube rings.as well as mono ring layer. Assignments of the modes of vibration were done depending on the pictures of their modes applying by Gaussian 03 program. The whole relations for the vibration modes were also done including (CH stretching, CC stretching, deformation in plane of the molecule (δCH, δring and δCCC), deformation out of plane of the molecule (CH and
... Show MoreDuring period from September 2012 to August 2013, concentration of total Trihalomethanes (TTHMs) was assessed in drinking water in Al-Dora and Al-Rasheed purification plants. 216 samples were collected from final basins of chlorination in purification plants and from sites that distributed among residential areas fed by the project. TTHMs concentration did not exceed (0.15 ppm) which is the maximum limits according to Iraqi standard specification for drinking water. The highest value was in July (0.12 ppm) and the lowest value was in November (0.01 ppm).
The optical properties of the polyvinyl-chloride thin films were studied which
include their absorbance, transmittance, reflectance spectra, band gap, and refractive
index, before and after annealing at T = 75οC for 24hours . The films were found to
exhibit high transmittance, low absorbance and low reflectance in the visible, and
near infrared region up to 1100nm. However, the absorbance of the films was found
to be high in the ultra violet region with peak around 306nm. The dielectric
constant ε′, dielectric loss ε″, and ac conductivity of polyvinyl-chloride were
obtained at different frequencies and temperatures. The experimental results show
that ε′ and ε″ decreased with increasing
L-Asparaginase (E.C.3.5.1.1) is an important natural product that possesses a
broad spectrum of antitumor activity. In the present study, L-asparaginase partially
purified from local isolate of Escherichia coli that were grown aerobically for four
hours and anaerobically for 18hrs on M9 medium contain L-asparagine as sole
nitrogen source. Extraction of the enzyme was done by sonication. Qualitative and
quantitative assays for L-Asparaginase production were determined using
colorimetric and nesslerization methods respectively. The purification steps involve
dialysis of crude extract and DEAE-Cellulose Ion exchange chromatography. The
enzyme was purified 3.39-folds and showed a final specific activit
Six bacterial isolates belonging to the genus Pseudomonas were
selected for their high ability to utilize crude oil. These isolates showed a
diverge ability for utilization of different hydrocarbon compounds and 2
isolates (P. aeruginosa RB19 and Pseudomonas sp. RB29) were able to
utilize all the tested compounds except tetrahydrofuran, sodium toluate and
trans–1, 2–diphenylethylene. Conjugation between P. aeruginosa RB19
and P. aeruginosa RB27 was made in an attempt to obtain bacterial
isolates, capable to utilize a wide range of hydrocarbon compounds. The
conjugation was successful and a number of transconjugants were obtained.
All transconjugants were able to utilize sodium toluate, and