Thirty blood samples of clinically glucose-6-phosphate dehydrogenase (G6PD)-deficient patients were collected from pediatrics hospital and Al- Kadhumia teaching hospital in Baghdad, Iraq. .Besides that, blood samples were taken from 30 apparently healthy individuals (without Hemoglobinopathy disorders ( as a control group. The apoptotic response shown by G6PD-deficient patients was evaluated using neutral method of Comet assay. Comet assay result was demonstrated as comet index which represent a mean of the scored that calculate from the ratio of cell diameters (L/W) of total cells in each sample. These results revealed that lymphocytes exhibited an increase apoptosis percentage (44.5%) compare with percentage of control (7%). This revea

A new method is characterized by simplicity, accuracy and speed for determination of Oxonuim ion in ionisable inorganic acid such as hydrochloric (0.1 - 10) ,Sulphuric ( 0.1 - 6 ),nitric ( 0.1 - 10 ), perchloric ( 0.1 - 7 ), acetic (0.1 - 100 ) and phosphoric ( 0.1 - 30 ) ( mMol.L-1 )acids. By continuous flow injection analysis. The proposed method was based on generation of bromine from the Bro-3-Br-- H3O+. Bromine reacts with fluorescein to quenches the fluorescence . A sample volume no.1 (31μl) and no.2 (35μl) were used with flow rate of 0.95 mL.min-1 using H2O line no.1as carrier stream and 1.3 mL.min-1 using fluorescein sodium salt line no.2. Linear regression of the concentration ( mMol.L-1 ) Vs quenched fluorescence gives a corr

In this work, L-Triptophan was coupled with diazotized 4-aminoantipyrine to give novel Mono azo ligand 2-(4-aminoantipyren)-L-Tryptophane (AAT). This prepared ligand was coordinated with chloride salts of [Ln=La(III), Ce(III), Nd(III), Eu(III) and Gd(III)] ions. The structural feature of the ligand and it's metal complexes were identified by elemental analysis FTIR, HMMR, UV-Vis spectroscopy, magnetic susceptibility and molar conductivity. Chloride ion content was also appointed by Mohr method. The spectral data and physicochemical studies declares that the ligand act as neutral N,O-bidentat Pentagonal bipyramidal structure has been suggested for all prepared complexes which were exhibited (1:2) metal:ligand ratio at optimum condition as

Phenoxazine prepared by the reaction of o-aminophenol with Zinc chloride in the presence of phosphoric acid. This work comprises the synthesis of new phenoxazine derivatives containing heterocyclic moieties. These heterocyclic compounds were synthesized in three groups. The first group is made up of 3-(oxoalk-en-1-yl)phenoxazine derivatives (3a-3g) obtained from the reaction of 10-acetyl- phenoxazine with different aromatic aldehyde in the presence of sodium hydroxide. The other two groups involve compounds produced from the reaction of (3a-3g) with hydrazine hydrate in acetic acid to get 10-(pyrazolin-3-yl)phenoxazine derivatives (4a-4g) and hydroxyl amine hydrochloride in the presence of ethanosodium hydroxide to afford 10-(1-phenyl py

Pseudomonas aeruginosa was isolated from three soils contaminated with gasoline which leak from electricity generators that used in different regions in Baghdad. The regions choices to collect the contaminated soils were Al-Dora, Al-Jadryia and Tunis quarter. The bacterial isolate is identified according to international biochemical methods and through the identification and selection the P.aeruginosa in order to use to degrade the gasoline also it was found that the optimum temperature and pH were 35°C and 9 to the P.aeruginosa, as well as using Fourier transmission infrared (FTIR) technique in order to test the ability of isolated bacteria to biodegrade the gasoline in order to use this bacteria in biodegradation for contaminated soil

This study includes isolation, purification, and identification of Aspergillus niger
from different sources (soil, Seeds, powdered milk and factory waste water) by
traditional methods (macroscopic and microscopically). and study the relationships
of genetic among the A. niger isolates based PCR (RAPD) technique (find the DNA
fingerprint). The results of this study revealed that genetic diversity and
relationships among twenty four isolates were determined by using the random
amplified polymorphic DNA (RAPD) technique. Genomic DNA of each isolates
was extract at final concentration of (115-959) μg /l per (2-3) gm of wet mycelium,
and at a purity (1.7-1.9). Each DNA of isolates were amplified with e

The study was done in the Hospital of Ibn-AL-Hythem for eyes infections in Baghdad from July till October of 2014. 120 conjunctiva eyes swap samples were collected from patients with pinkness or redness of the conjunctiva, which include 59 male and 61female. The diagnoses of specimens were done in the laboratories of the hospital by using the Analytical Profile Index System (API system). The results showed that 29(24.1%) samples were positive for bacterial infections while 91(75.9%) samples gave negative results, which mean conjunctivitis occurs by other causes. The study showed low in the percentage of bacterial infections that occurred in the eyes lid conjunctiva as well as in the per- orbital region. The predominant isolated species w

Eighty one bacterial isolates were obtained from 53 soil samples of different plants rhizosphere. All the isolated bacterial were screened for antifungal effect against Fusarium oxysporum. Three isolates gave antifungal activity with inhibition zone ranged between (0.5-2.5 cm). Two isolates (Bd1 and Bd2) were Brevundimonas diminuta, while the third(Pf1) was Pseudomonas fluorescence .B.diminuta(Bd1) which used in this study isolated from Raphanus sativus gave the highest inhibitionzone against F. oxysporum.In green house experiment , seedling of tomato cultivar Lycopersicon esculentum . var. commune were treated with two concentration 1012 cell/ml(B12 ) ,1014 cell/ml (B14) of B.diminuta (Bd1) and planted in soil mixed with106 spores/gm(F)

In the current study hundred ten of Iraqi patients with colorectal tumors were studied to evaluate the expression of Cathepsin D using Tissue microarray-Immunohistochemisty (TMA-IHC) technique. Of them, Ninety cases had colorectal carcinoma, and twenty had benign tumors. A group of twenty cases of non-specific colitis and other twenty colonic biopsies without significant pathology were also studied. Results revealed high expression of Cathepsin D in tumors (96.7% of malignant cases, 80% of adenomas) versus 30% of colitis. As well high significant differences (P< 0.001) in the expression of CathepsinD in different tumor types (adenocarcinoma and mucinous carcinoma), tumor stages and grades.

صُمِمت هذه الد ا رسة لمعرفة تأثير مستخلص الشاي الاحمر كمضاد أكسدة في ذكور الأ ا رنب البيض
المعرضة للإجهاد التأكسدي المستحدث ببيروكسيد الهيدروجين مع ماء الشرب. وقد استعملت في هذه الد ا رسة
24 حيواناً من ذكور الا ا رنب البيض Albino rabbits male , قسمت عشوائياً إلى 4 مجموعات تضمنت
كل مجموعة 6 حيوانات وت ا روحت أو ا زنها ما بين 1-1.5 كيلو, وبأو ا زن متقاربة , إذ تم معاملاتها بالمعاملات
المختلفة طيلة مدة التجربة البالغة

The present study was carried out on (80) adults asthmatic patients (43 male and 37 female), their age from (25 -65) years. Some of them acute episodes while other between episodes interval.They were selected from out patients clinics of AL-Zahra'a hospital for allergy and asthma in Baghdad.All patients were diagnosed according to standard medical criteria as being asthmatic. The results of investigations were compared with those for a total of (20) healthy individual [(12) male & (8) female] with age (25 – 45) years, as a control group, throughout the period extended from ( July 2014 till December 2014).
The patient samples were applied to determine complete blood count include (Hb, PCV, RBC count, WBC & Eosinophil count)

Myiasis is abnormal case of man infection due to either ovulating flies in the wounds which called cutaneous myiasis or due to ingestion of eggs of the flies accidentally with food which causes the gastric or intestinal myiasis .We report here a case of myiasis in a mature male (60 years old) sent by a private clinic in Baghdad to parasitological section /Central Public Health Laboratories (CPHL) /Baghdad and by the help of Museum of Natural History, it diagnosed as intestinal myiasis.

A gleevec drug a tyrosine kinase inhibitor that targets the so-called Philadelphia chromosome that characteristic of chronic myeloid leukemia (CML) which has been widely used in treatment of several hematological malignancies, and there is concern about the long-term immune effects of its use. Autoimmune disorders in patients treated with gleevec drug may be related to the direct immune modulating properties or may be linked to a possible toxic effect in target organs, triggering autoimmunity diseases. The study described the development of symptomatic autoimmune thyroid peroxidase antibodies (TPO) in 51 patients with CML compared with 10 subjects as healthy control. The samples collected from Iraqi National Center for Research and Treat

An investigate of the subsurface of East Baghdad Oil field South-1 and South-2, by using the interactive workstation Geoframe. The study is interested with Oligocene Formations which is an extension of Kirkuk group. Synthetic seismogram is generated from data of EB-15 well. Base of Lowe-Fars reflector is picked and identified because it is close to Jeribe Formation that was deposited in the same depositional environment of Kirkuk group. The seismic sections and time slice maps confirmed that the Oligocene age was not affected by faults and the signs or indicators of faults started from Hartha Formation and continue to the deeper formations with increasing intensity, where the variance attribute section was applied on time slice and shows

Thirty one isolates of P. aeruginosa were collected from 100 clinical samples include (pus, burn and wound swabs). All the isolates were identified as P. aeruginosa by conventional methods, PCR and DNA sequencing. Antibiotic sensitivity tests were undertaken for 8 groups of antibiotics (Cephalosporins, Rifamycin, fluoroquinolones, Penicillins, Carbapenems, Aminoglycosides, Chloramphenicol and Beta-lactamase inhibitor+ Penicillin). The highest resistance rate was recorded to Rifampin, and Pipracillin with 31(100%), followed by Chloramphenicol ,Cefotaxime and Cefixime with 28 (90.3%), and the lowest resistance rate to Imipenem with 8 (25.8%). Cefpodoxime resistance value was less than values reported in other study. Six strains did not car

A series of new derivatives of imidazo[2,1-b]naphtha[2,1-d][1,3]thiazole were synthesised, which they are substituted at position 2. Then, the new prepared imidazo thiazole derivatives were reacted with N,N-dimethyl formamide (DMF) and POCl3 to obtain new aldehyde derivatives at position 3. The new aldehyde derivatives underwent condensation reaction with the hydrazine afforded new hydrazone derivatives. The new compounds were characterized by spectra [IR, 1HNMR, 13CNMR, 2D NMR ( hsqc spectra)]. The synthesised compounds were examined the antimicrobial and antifungal activities, their antimicrobial activities against Escherichia coli, Streptococcus faecalis, Aeromonas hydrophila and Staphylococcus aureus were examined. Most of these comp