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Our objective in this work was to estimate normal values of biochemical parameters in laboratory animals in Iraq. Values were pooled from studies conducted in Iraqi universities using a meta-analysis approach. Pertinent peer-reviewed published studies were retrieved from the Iraqi Academic Scientific Journals (IASJ) database. Random-effects inverse-variance model was used to obtain pooled means of the parameters from the set of studies qualified for the analysis. Among a total of 460 records identified from IASJ, 264 records were eligible for the statistical analysis, which reported 102 values for different biochemical parameters. The mean of the sample size for normal animals that was used in the studies in |
Ginger (Zingiber officinale Rosc.) is a traditional plant that is widely used as a spice or folk medicine. Lambda-cyhalothrin (LCT) is a synthetic pyrethroid that is widely used to control insecticide. The present study aimed to evaluate the potential protective effect of ginger ethanolic extract (GEE) on liver toxicity experimentally induced by LCT in albino rats. The experiment involved thirty adult male rats (Rattus norvegicus), randomly allocated to one of three groups (n=10/group: control group, administered distilled water orally for 12 weeks; LCT-treated group, received 5.43 mg/kg BW (1/15 LD50 dose calculated in this study as 81.5 mg/kg BW) orally, for 12 weeks; LCT-GEE-treated gr
... Show More Isolation &identification of Newcastle disease virus from wild pigeons
inhabiting University of Baghdad (Al-Khwarizmi College of engineering)
where velogenic and devastating infection of birds was encountered. Chicken
embryos fibroblasts (CEF) were used for virus isolation (from infected
spleen and lungs) and propagation. Viral cytopathogenic effect was noticed
on infected (CEF) cell culture within (24) hr post inoculation. The virus was
identified by heamagglutination inhibition (HI) test using reference anti
NDV serum.
(1)
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Growth plate injury is one of the most significant sequelae that affect the animal and man life in adulthood. This condition considered as one of the real problem encountered by many orthopedics which can result in the formation of a bony bridge leading ultimately to limb length discrepancy and angular deformity of the long bones in kid animals. The objective of this study was to evaluate the deformities of the tibial length and angle radiographically after experimentally inducing growth plate injury. Five local breed kit rabbits included in this study, had body weight and age ranges of 500 to 1100 g and 6 to12 weeks, respectively. The animals were exposed to approximately 5×5×1 mm injury in the prox |
Out of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty
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This experiment was carried out at poultry house, college of Veterinary Medicine, Baghdad university,
Which lasted 42 days, to by adding Thymus vulgaris the used part (Leaves, Steams and Flowers) and
Cinnamomum zeylanicum the used part (bark) to the concentrate diet for the experimental period for 6 weeks.
The study was aimed to find out the effect of thyme or cinnamon on productive, physiological blood traits of
broilers by using 150 unsexing Arbor Acres chicks at the age of one day. These chicks were randomly divided
into five group equally (30 chicks each). Each group was subdivided into two equal subgroup. And fed on the
following ration.
1-The first group was fed on ordinary diet kept as a control group.
2- T
(2)
Monolayer tissue cultures of chicken embryo fibroblast ( CEF ) cells infected with avian
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Type 2 diabetes mellitus (T2DM) in dogs is a complex, multifactorial disease that is characterized by chronic hyperglycemia and insulin resistance. Current therapeutic options are often limited by side effects and variable efficacy, highlighting the need for more effective and safer treatments. This study assessed the therapeutic potential of ginger ethanolic extract (GEE), GEE-loaded chitosan nanoparticles (GEE-CNPs), and chitosan nanoparticles (CNPs) against T2DM in dogs. Twenty adult local breed mongrel dogs of both sexes, aged 7 to 13 months, with an average body weight of 10.4±0.76 kg, were included. The dogs were allocated into five groups (n=4 each): a non-diabetic, untreated Negative Control group, |
The study was carried out to evaluate histological changes induced by Aluminum chloride in Wistar rats. Forty rats were uniformly divided into two different groups: The control group were given distilled water during period of experiment and the first group were given oral daily dose of Aluminium chloride (37 mg\kg B.W). After 60 days of treatment rats were sacrificed. Liver, kidney and spleen were processed and stained with Haematoxylin and Eosin the lesions of these organs were characterized by degeneration, necrosis, and congestion which meant that Aluminum chloride was nephrotoxic as well as hepatotoxic in nature. Microscopic examination results showed that Aluminum chloride exposure was harmful to the histol
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(2)
هدفت الدراسة التحري عن بكتريا المتحركة عترة Aeromonad من اسماك جلبت من حقول لاسماك
الكارب حول بغداد وشخصت العزلة باستعمال عدة تشخيصية جاهزة . .API20E تم احداث اصابة تجريبية
بحقن الجرعة المميتة LD50 0.3 ×108. 66cfu/ml من العزلة المرضية في داخل العضلة لاسماك الكارب
الشائع Cypnuris carpio L ظهرعلى الاسماك بعد 41 يوم من الحقن نزف على سطح الجسم, تقرح مع بروز
العين وتجمع سوائل حمراء في منطقة البطن. عيانيا ظهر وجود بقع نزفية وتنخرية عل
(3)
Mastitis were diagnosed in (80) ewes depending on clinical examination , California mastitis test , & bacterial isolates inclu
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The estrous cycle of 8 Arabian mares was followed for one year, by visual observation of the estrous behaviour and by teasing with a stallion. Serum progesterone concentration was determined by RIA, Vaginal electric resistence was studied with aid of Ovascan, comapared with the changes in the reproductive tract as determined by rectal examination for prediction of ovulation. The changes in the reproductive tract and the behavioural manifestations expressed during the cycle indicated that the experimental mares were exhibiting estrous year arround, however, 75% of the cycles were observed during the months from March to August and 25% during the. rest of the year. The total number of cycles exhibited by the mares averaged 68 cycles, of th
... Show MoreThe study was conducted to investigate the effect of different concentrations of sodium hypochlorite (sodium dichloro iso cyanurate) in the drinking water on the percentages of serum proteins of broilers chicks at 4 and 7 weeks of age. Two hundred forty Hubbard chicks one day – old aged were divided randomly into four equal groups, each group was divided into two replicate. The four groups were as follows: Treatment 1: Adding 6.68 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 2: Adding 13.36 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 3: Adding 20.04 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 4: Supplying water without any additive (con
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The histological study showed that the retina of falcon"s eyeball was thin at the periphery
and ranges between 0.6-0.8 μ but it was thick at center 1.68-2.64 μ. The retina consists from
ten layers: pigmented epithelium, rods and cons layer, external nuclear layer, external
plexiform layer, internal nuclear layer, internal plexiform layer, Gangilionic cells layer,
neurofibres layer and internal limiting membrane layer. The ultra-structural study stated that
the rods and cons layers contained single rods with single and double cons. The retina
characterized by lacking of the oil droplets in the internal segments of rods with narrow
external plexiform layer in order to form network connecting rods and cones, horizonta
This trial comprised (28) naturally affected calves with hypomagnesaemic tetany, which were diagnosed clinically and by measuring the serum level of magnesium, calcium,
Fate of AflatoxinM1 in soft white cheese and its by-product (whey) and in yogurt locally made from raw sheep's and goat's milk experimentally inoculated with 0.05 and 0.5 µg/l AflatoxinM1 were investigated using ELISA technique. Results reported that AflatoxinM1 was concentrated in cheese at levels significantly higher than that recorded in the raw milk that used for its processing, with a significant decrease in AflatoxinM1 levels in its by-product (whey) comparable to the raw milk used in manufacturing at both inoculated levels. Yogurt produced from raw sheep's milk at second inoculated level exerted AflatoxinM1concentration significantly lower than that present in the milk. Significant differences in Aflatoxi
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A total of 69 fish samples were collected from three stations Tigris River namely (Al-Zaafaraniya, Al-Tagei and Al-Shawaka) at Baghdad city, during the period from January to December 2010). These fishes were belonging to five species which were Barbus luteus, Carassius carassius, Chondrostoma regium, Liza abu and Silurus triostegus. The microscopial examination revealed infection with 39 species of ectoparasites and endoparasites including twenty one from protozoans (five ciliate (E.cyprini, E.dogieli, E.spherica, T.domerguei, T.nigra) and sixteen sporozoa (C.bychowski, Myxidium monstrasum, M.pfeifferi, M.rhodei, Myxobolus bramae, M.cyprini, M.cyprinicola, M.drgajini, M.koi, M.macrocapsulari, M.mulleri, M.oviformis, M.paljanski, M.parvu
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The developing ectopic adrenal cortices from nodules. The nodules are embedded in the adrenal capsule
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This study was conducted on local breed goats to determine the changes in biochemical parameters in anemic cases by using morphological classification, one hundred and seventyserum samples were collected randomly from goats of both sexes aged 3 months – 6 years, they were grazing on pasture from the different districts of Baghdad, Bakuba and Falluja. The total protein, albumin and fibrinogen in the normal bucks were 73 1 – 84 1 g/L, 35 3- 37 1 g/ L, 9 8- 11 1 g/ L, respectively and in the normal does were 66 1 – 77 4 g/ L, 35 6 – 37 2 g/ L and 6 – 15 g/ L, respectively, the average of these parameters differed significantly at (p> 0.05) between the normal and the anemic animals. The mean level of copper in the serum of norma
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This study was conducted to characterize the pathological changes caused by F. gigantica.
The pathological changes were studied in samples collected from abattoir, which included, livers, gall badders and hepatic lymph nodes.
The results indicate that the disease is endemic in Baghdad area and the areas around it. The incidence rate of this parasite increased in the beginning of winter and decreased to it's‘ lowest in April. The disease(Fascioliasis) was classified as acute and chronic.
This study was planned to isolated and identify some psychrotrophic aerobic sporeforming bacteria from
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The present study aimed to identify the efficacy of Brucella melitensis vaccine strain Rev 1 in induction of antibodies by different doses and routes of administration; also to obtain the specificity of the serological tests, the study included 28 ewes divided into four equal groups . the animals of first and second groups vaccinated subcutaneously with 2×109 and 2×107 colony forming units (CFU) respectively while the animals of third and fourth groups vaccinated conjunctively with 2×109 and 2×107 CFU respectively . The sera were collected at zero time, 2, 4, 8, 16, 20, 24 and 28 weeks of the experiment and the antibodies response were evaluated using classical tests ( Rose Bengal, serum agglutination and 2-mercaptoethanol tests ) co
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Lymphoma is a cancer arising from B or T lymphocytes that are central immune system components. It is one of the three most common cancers encountered in the canine; lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, such as lymph nodes, lymphoid tissue, or spleen. Despite the advance in the management of canine lymphoma, a better understanding of the subtype and tumor aggressiveness is still crucial for improved clinical diagnosis to differentiate malignancy from hyperplastic conditions and to improve decision-making around treating and what treatment type to use. This study aimed to evaluate a potential novel biomarker related to iron metabolism, em |
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This work aimed to use conventional PCR to identify Salmonella spp. that were isolated from diarrheal children and healthy and diarrheic dogs based on four virulence genes, hilA, stn, spvR, and marT. Sixteen Salmonella isolates including: 9 isolated from children's diarrhea from three species (S. Typhimurium, S. Enteritidis, S. Typhi) and seven isolated from dogs including (S. Typhimurium, S. Enteritidis, S. Muenchen), were identified primarily by several methods. The PCR products of the 16S rRNA gene were sequenced and examined using |
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Aflatoxin B1 (AFB1), ochratoxin A (OTA), and fumonisin B1 (FB1), the most commonly encountered mycotoxins, constitute serious human and animal health threats as a result of their toxigenic, carcinogenic, and mutagenic influences. The study aimed to investigate the occurrence of these mycotoxins in poultry feeds and determine the percentage of the samples that exceeded the legal limits approved by the European Commission (EC). Sixty poultry feed samples were collected from poultry feed plants and poultry farms in Nineveh Province and analyzed for detection mycotoxins using competitive Enzyme-Linked Immunosorbent Assay (ELISA). Results reported co-occurrence of AFB1 and FB |
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Cypermethrin (CYP), a synthetic pyrethroid, is recognized for its insecticidal properties but poses potential risks of hepatotoxicity. In traditional medicine, Ficus (F.) carica (common fig) leaves have historically been used for various therapeutic applications. This study aimed to evaluate the hepatoprotective effect of the methanolic extract of F. carica leaves against CYP-induced liver damage in adult male albino rats (Rattus norvegicus). The animals (n=30), 8-12 weeks old and weighing 200-250 g, were randomly divided into five experimental groups (n=6) and treated as follows: the negative control group received d |
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Mercuric chloride (HgCl2) pollution and poisoning has been a worldwide health concern for decades, especially after the industrial revolutions. The aim of this study was to investigate the role of resveratrol in reversing the deleterious effects of HgCl2 exposure to resume the normal functions of hepatocyte. To achieve the study, mature Sprague Dawley rats were assigned to five groups. Negative control group (C) kept without any treatment; vehicle-treated group (D) received dimethyl sulfoxide (DMSO); resveratrol-treated group (R), received 100 mg/kg of resveratrol; HgCl2-intoxicated group (HD), received i.p. injection of HgCl2 at a dose of 1 mg/ |
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The aim of this study was to prepare and characterize a small intestine submucosa (SIS) hydrogel as a bio-scaffold. In this study, SIS from five calves, aged 8-12 months and weighing 250-300 kg, was obtained from a slaughterhouse immediately after slaughtering. The SIS was then decellularized, powdered, and subsequently transformed into a hydrogel. This transformation was achieved by dissolving the decellularized SIS powder in phosphate-buffered saline (PBS) at a concentration of 50% w/v, and allowing it to form a hydrogel over a 12-hour period at 37 °C. Characterization of the SIS hydrogel was conducted using various techniques. Fourier Transform Infrared Spectroscopy (F |
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The aim of this study was the discrimination of Salmonella isolated from chicken and their feed and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, and serology were used for bacterial identification. Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships among Salmonella isolates. The isolation rate of Salmonella spp. was 21.1% (61/289). While |
A study on the prevalence of gastrointestinal parasites of horses in Baghdad area extending over a period of one year , revealed two small peaks in worm egg counts , one in spring and the other in autumn and treatment routines should be based on this information. During the hot and dry summer months of Iraq, helminth infection was at low level.
Faecal culture for larvae and their identification indicated the predominance of small red worm infection in the horses.