Background: The Apgar score is calculated based on the five features: heart rate, reflexes, color, muscle tone, and respiratory effort. Each element is scored from 0 to 2, with a total probable score of 10 at 1 minute and 5 minute. Aim: To study the influence of gestational diabetes mellitus (GDM) and diabetes mellitus on APGAR score in neonate. Materials and methods: The samples were studied from Department of Obstetrics and Gynaecology in Al-Imamain Al-Kadhimiyain (AS) Medical city, Baghdad Teaching Hospital and Al-Karkh Maternity Hospital in the period between 1 December 2016 and 1 may 2017, after obtaining the approval from Iraqi Ministry of Health. A total of 102 neonates were included in this study which includes 34 neonates of mothers with gestational diabetes (GDM), 34 neonates of mothers with pregestational diabetes (DM) and 34 neonates of mothers with normal pregnancy as control group. Results: APGAR score of neonate (after 5 min) have been highly significant (P≤0.001) among three groups. Therefore, maternal history of GDM and DM appear to be associated with the after5 minute APGAR score of neonates with GDM and DM mothers as compared to neonates of normal mothers. Conclusion: The neonates of diabetic mothers are lower APGAR score than that of normal mothers, this due to maternal hyperglycemia and fetal hyperinsulinism.