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ESTIMATION OF GENETIC VARIATIONS IN DIFFERENT TAXA IN BRASSICACEAE BY RAPD AND ISSR ANALYSIS

Twelve species from Brassicaceae family were studied using two different molecular techniques: RAPD and ISSR; both of these techniques were used to detect some molecular markers associated with the genotype identification. RAPD results, from using five random primers, revealed 241 amplified fragments, 62 of them were polymorphic (26%).

 

    ISSR results showed that out of seven primers, three (ISSR3, UBC807, UBC811) could not amplify the genomic DNA; other primers revealed 183 amplified fragments, 36 of them were polymorphic (20%). The similarity evidence and dendrogram for the genetic distances of the incorporation between the two techniques showed that the highest similarity was 0.897 between the varieties red cabbage and red ornamental cabbage, meanwhile the lowest similarity index was between the varieties red radish and green ornamental cabbage (0.169); thus these RAPD and ISSR markers have the possibility for the identification of species or varieties and the description of genetic variation within the varieties. Furthermore, it could be concluded that the Brassicaceae taxa have a suitable amount of genetic variance and a wide range in the genetic principle of the studied genotypes which can be used for output improvement.

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Publication Date
Wed Feb 22 2023
Journal Name
Iraqi Journal Of Science
Molecular Identification of Aspergillus fumigatus Using ISSR and RAPD Markers

The aim of this stud to isolate and identified of A. fumigatus from different sources and study the genetic diversity among these isolates by using RAPD and ISSR markers.Collected 20 samples from 7samples were isolated A. fumigatusisolates were characterized depending on its morphological, then extracted DNA from its.RAPD markersrandomly bandingwith sitesof genome more than ISSR markers where the primer OPN-07 achieved discriminative power (19.1) and 43 bands, while ISSR6 achieved discriminative power (17.1) with 32 bands.ISSR were more efficiency in specific binding then RAPD, ISSR primers has great a binding to production unique band, when 9 primers from 01 primers, ISSR9 was produce (5) unique bands, while RAPD markers was low ability

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Publication Date
Sun Mar 04 2018
Journal Name
Iraqi Journal Of Science
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Publication Date
Tue Mar 14 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Genetic Diversity of Iraqi Barley Species Differing in Their Tolerance to Drought by RAPD Analysis

The genetic diversity was studied in sixteen barley Hordeum vulgar L. species cultivated in Iraq , which are differ in their ability to drought stress tolerance by using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR ) .Barley species was evaluated to drought stress  after treatment the plant seedling at germination stages to different concentration of polyethylene glycol (PEDG6000) . The results showed that the Broaq and Arefat species have the highest tolerance to drought stress in contrast the rest of Barly species like Alkhair, Alwarkaa, Ebaa99, Shoaa, Alrafidain,Sameer Rehana 3 , forat9 , jazeral ,and ebaa7 revealed sensitivity to drought stress .      The primes which used RAPD technique

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Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Genetic diversity of Capsicum annuum L. in local and imported samples in Iraq by using RAPD-PCR

Genetic variation was studied in 22 local and imported samples collected from local Iraqi market by using random amplified polymorphic DNA (RAPD-PCR). Five randomly primers set were used in this study. These primers produced 292 bands. Molecular weights of these bands ranged between 1.8 Kb (1800 bp) to 150 bp. The percentage of polymorphic bands is 100%, with one distinguished band which is produced by using C52 primer. The other primers did not produce any distinguished band. The results of Dendrogram of the studied samples depended on RAPD-PCR results by using Jaccard coefficient for genetic similarity was distributed the samples into 8 groups. This Dendrogram revealed a higher similarity between Iraqi/Yousifia green bell pepper and Jo

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Publication Date
Sat Mar 01 2014
Journal Name
Glob. J. Mol. Evol. Genom
Publication Date
Mon Nov 01 2021
Journal Name
Iop Conference Series: Earth And Environmental Science
Genetic Analysis by Using Partial Diallel Crossing of Maize In High Plant Densities (Estimation GCA, SCA and Some Genetic Parameters)
Abstract<p>The experiment was carried out at the Field Crops Research Station, College of Agricultural Engineering Sciences - University of Baghdad in Jadiriyah, with the aim of evaluating the performance of partial diallel hybrids and inbred lines of maize and estimating general combining ability(GCA), specific combining ability (SCA) and some genetic parameters. The experiment was carried out in two seasons, spring and fall 2020. Eight inbred lines of maize were used in the study (BI9/834, BSW18, LW/5 L8/844, ZA17W194, Z117W, ZI17W9, ZI7W4), numbered (1,2,3,4,5,6,7,8), It was sowed in the spring season and entered into a cross-program according to a partial diallel crossing system to obtain tw</p> ... Show More
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Publication Date
Mon Apr 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Genetic Diversity of Iraqi of Common Reed Phragmites australis by Using RAPD Technique

   Genetic diversity was studied in 31 Iraqi common reed samples , which were collected from Iraqi marshes in Basrah , Messan and Thi-Qar provinces and also from different areas in Baghdad province . Random amplified polymorphic DNA (RAPD) technique was used for evaluation of genetic diversity between collected samples . Seven primers were used for polymorphism detecting between common reed samples . The results revealed 102 bands for the all samples when RAPD-PCR was used . The percentage rate for the monomorphic bands is 6.86% , while the percentage rate for the polymorphic bands is 93.13% , and the numbers of these bands are ranging between 10 to 17 for each used primer .  The UBC1 primer gave the highest number of poly

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Genetic Diversity Among Some Aspergillus flavus Isolates by Using Inter simple sequence repeats (ISSR)

Aspergillus flavus isolates which are considered on conidial shape through microscopic examination and mycelial colour through cultural properties . Primary screening for the ability of A. flavus isolates for aflatoxin production was determined using A.flavus parasiticus agar medium (AFPA) as well , 7 isolates from 11 isolates give a positive result by the presence of bright yellow-orange pigments indicated the presence of aflatoxins. Molecular genetics techniques using DNA polymorphism have been increasingly used to characterize and identify genetic diversity and relationships among eleven A. flavus isolated from different source using the ISSR(inter simple sequence repeats) technique. Three universal primers designed at University of B

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Publication Date
Tue Feb 28 2023
Journal Name
Iraqi Journal Of Science
Genetic Diversity of Trichoderma and Hypocrea lixii Iraqi Strains by Using Random Amplified Polymorphic DNA (RAPD) Technique

       As the diversity and characteristics of Trichoderma species are difficult to determine using morphological methods, henceforth molecular tools are crucial. This study utilized random amplified polymorphic DNA (RAPD) technique to investigate the genetic diversity of Trichoderma with sexual phase Hypocrea and to identify similarities and differences in the phylogenetic tree. Nine Iraqi Trichoderma strains (four strains of T. atroviride, one strain of Hypocrea lixii, two strains of T. gamsii and two strains of T. longibriantium) were examined in this research. The genomic DNA of each species was extracted and amplified with each of the fiv

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Publication Date
Thu Mar 30 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Genetic Variations of Echinococcus granulosus Isolated from Sheep and Cows by Using Fingerprint DNA Method in Iraq

The fingerprinting DNA method which depends on the unique pattern in this study was employed to detect the hydatid cyst of Echinococcus granulosus and to determine the genetic variation among their strains in different intermediate hosts (cows and sheep).  The unique pattern represents the number of amplified bands and their molecular weights with specialized sequences to one sample which different from the other samples.   Five hydatitd cysts samples  from cows and sheep were  collected, genetic analysis for  isolated DNA was done using PCR technique and  Random Amplified Polymorphic DNA reaction(RAPD) depending on (4) random primers, and the results showed:      

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