The study aimed to survey mushroom species from fields among herbs, palm trunks, and
trees in central Iraq and to identify them on the basis of morphological and molecular
characteristics. As a molecular identification result with polymerase chain reaction six
species were recorded (with eight isolates): Agaricus bitorquis (Quéllt) Saccardo. 1887
(SHA14); Candolleomyces candolleanus (Fr.) D. Wächter & Melzer, 2020 (SHA15);
Cyclocybe cylindracea (D.C.) Vizzini & Angelini, 2014 (SHA13); Leucoagaricus hesperius
Vellinga, 2010 (SHA16); Volvariella sp. (SHA17), and Volvopluteus gloiocephalus (D. C.)
Vizzini, Contu & Justo, 2011 (SHA10, SHA101 and SHA12), belonging to four families of
Basidiomycetes: Agaricaceae, Pluteaceae, Psathyrellaceae, and Strophariaceae within the
order Agaricales. The nuclear sequences of these species have been deposited in GenBank
under the accession numbers: PP576693, PP578967, PP576221, PP576960, PP580694,
PP575891, PP580693, and PP576069, respectively. These species were recorded for the first
time in these areas in the middle of Iraq.