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An Experimental Study of Collection ,Separation , Enumeration and Cryopreservation of Umbilical Cord Blood Stem Cells
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   Umbilical cord blood (UCB) contains hematopoietic and mesenchymal stem cells(HSCs,MSCs) that have proven useful clinically to reconstitute the hematopoietic system in children and some adults  .   Fifteen cord blood samples were collected from placenta of  newly delivered women in AlKadhemia hospital in Baghdad for normal vaginal delivery.      Mono nucleated cells (MNCs) were isolated by using density gradient centrifugation and the MNCs  count and viability  were  determinated by trypan blue.MNCs were cryopreserved using the cryoprotectant solution of 10% concentration of dimethyl sulfoxid (DMSO)using slow cooling and rapid thaw.  The aim of the present study is to collect, separate and enumerate the stem cells in cord blood(CB) and study the effect of cryopreservation on stem cells count and viability.   The results of the present study showed that the average cord blood volume collected was 81.66 ml. Mononuclear cell count ranged from 5.6-14.6 x106.Viability count of mononuclear cells was 93.9%.After three months of cryopreservation , the viability count on revival was 73.9%.This study represents an attempt to isolate  the stem cells from UCB.  The results of the present study  confirmed that  UCB provides a great source of stem  cells for  using in medical applications.  

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Publication Date
Sun Jul 21 2024
Journal Name
Cureus
The Effect of Maternal Blood Glucose on Umbilical Cord Blood Fibrinogen in Women With Gestational Diabetes
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Publication Date
Sun Apr 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Umbilical Cord Care in the Newborn Infant.
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Background: The umbilicus, before and shortly after sloughing of the cord, can be a source of infection or toxicity. The infection can be severe, such as septicaemia
or tetanus neonatorum.
Method: Mothers of 200 neonates seen at the Children Welfare Hospital, outpatient department, Baghdad, and at Al-Takia health centre, Baquba, were interviewed about the separation of the umbilical stump and the care of the umbilicus after delivery at home or hospital, in the period, January to July 2003, excluding March and April, the time of active military invasion of the country.
Results: For occlusion of the cord a plastic clamp was used in all hospital deliveries, and 80% of home deliveries. A cotton thread or a s

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Publication Date
Sun Apr 02 2006
Journal Name
Journal Of The Faculty Of Medicine Baghdad
nucleated red blood cells in cord blood of singleton term neonates and the risk factors affecting their count
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Background:

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Publication Date
Sun Apr 02 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Umbilical cord drainage versus intraumbilical cord oxytocin injection in management of third stage of labour
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Background: Postpartum hemorrhage is an important cause of maternal morbidity and mortality. Considerable difference of opinion exist regarding the optimal approach to the management of the 3rd stage of labour, practice varies between countries &between units.
Objectives: To evaluate the effectiveness of intra umbilical vein injection of oxytocin and umbilical cord driange in shortening the duration of third stage of labour.
Patient and Methods: In this randomized controlled study, 100 women were enrolled in this study they divided into three groups. (Group 1 ,N =30 )received 20 units of oxytocin diluted in 20 ml 0.9% saline solution injected in the umbilical vein after clamping.(Group 2, N = 34) placental cord drainage.(Group

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Crossref
Publication Date
Sun Apr 02 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Umbilical cord drainage versus intraumbilical cord oxytocin injection in management of third stage of labour
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Background: Postpartum hemorrhage is an important cause of maternal morbidity and mortality. Considerable difference of opinion exist regarding the optimal approach to the management of the 3rd stage of labour, practice varies between countries &between units.Objectives: To evaluate the effectiveness of intra umbilical vein injection of oxytocin and umbilical cord driange in shortening the duration of third stage of labour.Patient and Methods: In this randomized controlled study, 100 women were enrolled in this study they divided into three groups. (Group 1 ,N =30 )received 20 units of oxytocin diluted in 20 ml 0.9% saline solution injected in the umbilical vein after clamping.(Group 2, N = 34) placental cord drainage.(Group 3,

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Publication Date
Mon Dec 23 2019
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Use of Human Umbilical Cord Serum to Treat Animal Skin Burns
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Objective: The objective of the study was to test the hypothesis, that umbilical cord blood crude serum applied topically with its high concentration of growth factors may promote an early healing for animal models with burn injury.

Methods:  Fresh human umbilical cord blood UCBS was collected and screened for transmitted diseases such as hepatitis B, hepatitis C and HIV. Mice and rabbits were scalded by boiling water and chemical burning by NaOH, then treated with UCBS. In comparison with a Cetrimide treated group and a control group (without treatment). The UCBS was subjected to microbial testing to demonstrate the presence or absence of extraneous viable contaminating microorganisms.

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Publication Date
Mon Apr 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Man made Stem cells
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stem cells are cells found in most of not all, multicellular organisns. these cells are characterized by the ability to self-renew through mitotic divisions and to differentiate into diverse range of specialized cell types to form a whole organism.

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Publication Date
Thu Dec 19 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Immunohistochemical study of CD34 in tooth eruption by using amniotic stem cells
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Background: Tooth eruption is a more general process, however, which includes certain posteruptive tooth movements. There are two fundamental requirements for both tooth eruption to occur: (1) Require soft tissue, intervening between tooth structure and alveolar bone, which plays an important role in regulating the remodeling of adjacent tissues. (2) Require bone turnover that is temporally and spatially regulated to facilitate specific translocations of teeth through alveolar bone These amniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for human application and recently it used in many medical branches. CD34 is an endothelial marker that is extensively used in immunohistochemistry a

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Publication Date
Wed Jul 10 2019
Journal Name
Pakistan Journal Of Medical Sciences
Maternal and cord blood prolactin level and pregnancy complications
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Objectives: To explore the correlation between maternal and cord blood prolactin, the correlation between cord prolactin and birth weight, and to compare cord blood prolactin in new-borns of women with normal pregnancy and women with pregnancy complications namely; gestational hypertension, gestational diabetes and preterm labour.Methods: This study was performed from September to December 2018. Thirty-two women, delivered at Baghdad teaching hospital, and their newborns (32) were included. Maternal blood (5 ml) was taken before labour and cord blood (5 ml) was collected after placenta expulsion. Maternal and cord blood prolactin were analysed using fluorescence immunoassay. Results: Cord blood prolactin was higher in babies b

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Publication Date
Thu Jun 01 2023
Journal Name
Baghdad Science Journal
In vitro isolation and expansion of neural stem cells NSCs
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   Neural stem cells (NSCs) are progenitor cells which have the ability to self‑renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as successful sources for further studies before using it for clinical trials. In this study, mouse bone marrow was the source of neural stem cells. The results of morphological study and immunocytochemistry of isolated cells showed that NSCs can be produced successfully and maintaining their self‑renewal and successfully forming neurosphere for multiple passages. The spheres preserved their morphology in culture and cryopreserved t

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