The aimed of present study is to shed some light on the immunological effect of soluble proteins extracted from Proteus mirabilis through employing the Specific Activity of Adenosine Deaminase : ADA The Soluble proteins; Spl and Sp2,was extracted by using the lysozyme enzyme The rabbits were divided into Three groups .the first one was injected with Spl, the second with Sp2 for four weeks .and the third one was control group. The results revealed that specific activity of Adenosine Deaminase was significantly increased in the second and fifth weeks of injected group with Sp1 compared with control group. The same effects of the Sp2 was observed in specific activity of Adenosine Deaminase. Therefore, the extracted soluble proteins had an effects on the immune response of the rabbits.