Amlodipine Besylate (AMB) was determined in pharmaceutical and biological samples using a continuous-flow injection technique characterized by simplicity, rapidity, cost-effectiveness, sensitivity, and a broad linear range. This study aims to develop and evaluate a flow injection analysis method for the rapid and reliable determination of Amlodipine in chemical and biological samples using minute quantities. The suggested method is based on the oxidation of the drug with a potassium bromide-potassium bromate mixture, followed by reaction with crystal violet as a reagent to produce a blue product, the absorbance of which was measured at 592 nm. The coefficient of determination  (R2) is 0.9978, and the calibration curve's linear range is 10-250 μg/mL. The limit of detection for the standard was 2.46 μg/mL, the recovery was 99.81%, and the relative standard deviation was 2.4%. The proposed method was successfully used to estimate AMB in biological and pharmaceutical samples. Compared with those obtained using a United States Pharmacopeia reference method, the results were favorable, and at the 95% confidence level, there was no discernible difference in accuracy and precision