Staphylococcus aureus is one of the causes of serious diseases that cause a wide range of infections, including urinary, skin, and respiratory infections. Thus, it has shown strains resistant to antibiotics, so that studying the molecular basis of virulence in staphylococcal bacteria becomes more important. The aim of this study is to investigate the presence of teicoplanin-associated genes (A, B, R) in        S. aureus bacteria taken from clinical samples by PCR technique. 25 clinical samples from patients with respiratory diseases, skin ulcers, and urinary tract infections were collected from Yarmouk Teaching Hospital for a period ranging from October 2024 to January 2025. The presence of          S. aureus in these samples has been identified by using standard biochemical methods and confirmed by the Vitek system. The resistance results showed that the highest percentage of resistance to the antibiotic was in benzylpenicillin (100%) and oxacillin (100%), and the lowest percentage of resistance was in the antibiotic (moxifloxacin 0%, linezolid 0%, tigecycline 0%, inducible clindamycin 0%, and trimethoprim 0%). purified the genomic DNA that was done by amplifying the polymerase chain reaction by specific prefixes of the target teicoplanin-associated genes (tca). The results indicated that all 25 isolates proved the presence of all tcaA, tcaB, and tcaR genes, and this was confirmed by gel electrophoresis. The relationship between the presence of genes with antibiotic resistance was detected; the result was shown especially for glycopeptides (vancomycin and teicoplanin).