C. albicans is diploid polymorphic yeast, which found as part of the normal microbial flora in the most of the healthy human. And it’s usually a harmless colonizer of mucosal surfaces; it can however cause disease in the case where the host suffers from weakened immune system or immunocompromised persons.  The present investigation was carried out to detection and investigates the prevalence of some virulence factors which include coagulase production, biofilm formation; phospholipase distribution among C. albicans isolated from different clinical samples include oral cavity and respiratory tract which collected from May to August during 2022 years numbered 280 of different age and sex of Iraqi patients with oral candidiasis and respiratory diseases.  And After detection and among the 102 positive sample results showed that 58(56.86%) sample from oral cavity and 44 (43.14%) was from respiratory tract. C. albicans was the most species prevalent among six type collected of Candida species while C. glabrata were the least. All the yeast were identified by different conventional methods including germ tube production, chlamydospore formation, grown on HiCrome Candida medium, and after which confirmed using VITEK-2 system. Biofilm activity was strong in 34 (48.57%) of C. albicans isolates. While 45(64.29%) strong isolates to produced phospholipase and 59(84.29%) isolates were had the ability to produce coagulase.