New complexes were synthesized from a newly prepared Schiff base ligand (L₂) derived from malonic acid dihydrizde and 2- pyridine carboxaldehyde. The 1H-NMR and ¹³C-NMR spectra demonstrated all the needed peaks to prove the chemical structure of the synthesized ligand (L₂). Utilizing mass spectroscopy, the molecular ion peak was found at m/e 310, confirming the formula weight for L₂, which matches the estimated m+ value (310.12). Accordingly, its Mn (II) and Co (II) complexes were trainedusing glacial acetic acid as a catalyst. These compounds were characterized by FT-IR, UV-Vis, C.H.N., chloride-containing, molar conductance, magnetic susceptibility, and atomic absorption. The characterization results gave complexes hexadentate coordination geometry for each cobalt and manganese complex. Schiff base ligand acted as tetradentate with a good yield. The biological activities of the new compounds were valued against two Gram-positive (Staphylococcus aureus and Bacillus subtitles), two Gram-negative (Escherichia coli and Pseudomonas aeruginosa), and candida fungi; hence, their results were good in inhibition.