The incidence and prevalence of type 2 diabetes mellitus is increasing worldwide due to hyperglycemia at long periods and not having a control on diabetes along with the presence of other risk factors. Diabetes complications include microvascular and macrovascular complications that target the kidneys, nerves, eyes and heart. Hence, the current study aimed to investigate the levels of (CTGF) and (TGF-B1) for both men and women and to demonstrate the effect of gender on it in addition to some related biochemical factors in patients with diabetes and diabetic nephropathy compared with healthy controls. The study included 120 male and female with age range of (30-65) years old. Ninety patients with type 2 diabetes  were subdivided into three groups on the basis of ACR criteria including normoalbuminuria, microalbuminuria, macroalbuminuria (30 patients for each group), and 30 healthy people served as the control group. All the individuals of the groups visited Baghdad Teaching Hospital / Medical City and Al-Yarmouk Teaching Hospital at the period between December 2021 and May 2022.  CTGF,TFG-B1 levels were determined using the ELISA technique.Urea results showed statistically significant differences between diabetic nephropathy in the patient group and the control group in female cases, but there were no statistically significant differences in male patients with diabetic nephropathy and the control group. The results also revealed that there were statistically significant differences in ACR, eGFR, urea, FBS and creatinine between diabetic nephropathy group and healthy group for both men and women.CTGF, TGF-B1 represent good markers for early prediction in diabetic nephropathy case.