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jih-2953
Anti-tumor Activity of Plantago lanceolata Aqueous Extract In Vitro and Genotoxicity by Micronucleus Assay In Vivo
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The study is designed to evaluate the effect of the aqueous extract of the P. lanceolata plant, as well as to know the effect of the drug CCl4 on the formation of micronucleus in vivo 48 female albino mice. In the study mice were separated into eight groups treated intraperitoneally for seven day first group Negative control, second positive control( CCl4 0.02%), third group aqueous extract (250 mg/kg), fourth group  aqueous extract (500 mg/kg), fifth group (CCl4 0.02%) plus aqueous extract (250 mg/kg), sixth group (CCl4 0.02%) plus aqueous extract (500 mg/kg), seventh group aqueous extract (250 mg/kg) plus (CCl4 0.02%), and eighth group aqueous extract (500 mg/kg) plus (CCl4 0.02%). The genetic-cellular aspect involved measuring the coefficient of micronucleus formation in bone marrow cells in mice treated with CCl4 and plant aqueous extract. The results showed that the treatment of mice with the drug led to a rise in the coefficient of micronucleus formation compared to the negative control group. In addition, it showed the plant's ability to reduce the drug CCl4 effect in the totals of overlaps between the plant extract and the drug at the concentrations used for the plant 250 and 500 μg/ml and reduce the formation of micronucleus.

The cellular toxicity of the plant’s aqueous extract on the liver cancer cell line was assessed in HepG2 (liver cancer cell line) and the WRL68 (hepatic human cell line) using concentrations (25, 50, 100, 200, and 400 μg /ml) from the plant’s aqueous extract on the HepG2 liver cancer cell line. The results showed a decrease in cell viability depending on aqueous extract concentration. The vitality of cancer cells decreased with the increase in concentration; the viability of the aqueous extract of the plant on cancer cells reached the minimum at concentration 400 μg/ml 45.34±4.44, while it reached the maximum when concentration  25 μg/ml 84.53±2.41.

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Publication Date
Wed Mar 29 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
In vitro MIC of Itraconazole Against Different Isolates of Candida albicans
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In vitro antifungal susceptibility test of itraconazole was carried out against 38 isolates from nails, skin, oral cavity, vagina and wounds, This study was done in Ramadi Teaching Hospital in period from January to August 2010. According to the National Committee for Clinical Laboratory Standard (NCCLS ) M 27- A by using the broth dilution method. Inoculum size was 1-5X103 CFU/ ml, while final concentrations of itraconazole ranged from 0.025 – 6.4 μg / ml by using RPMI – 1640 broth media and the fungus was incubated at 35 oC.  No resistant stain was recorded. MIC ranged from 0.05 – 6.4 μg / ml and the Mean ± SEM was 0.89 ± 0.28. MIC for nail isolates was 0.05 –

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Publication Date
Sun Jun 02 2013
Journal Name
Baghdad Science Journal
Inhibitory Effects of Chalcone on the Replication of Poliovirus in Vitro
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The compound chalcon originally is extracted form some plant and herbs, the studies of the antiviral activity of this compound were done in two cell line cultures the L2OB and RD, the compound relatively non toxic to both cell lines of the concentration of 32?g/ml or less ,the compound have significantly anti poliovirus activity in both L2OB cell line and RD cell line, we find that the concentration of 0.03 ?g/ml or more inhibit the 100TCDID50 of the poliovirus .The therapeutic index(TI)used in this study to evaluate the drug activity ,( TI is the ratio of dose of drug which is just toxic to the cells to the does which is just inhibit the viral multiplication, if this index more than one the margin of safety of drug is according great ) .In

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Publication Date
Thu Jun 01 2023
Journal Name
Iraqi Journal Of Physics
Cytotoxic Activity of CuO NPs Prepared by PLAL Against Liver Cancer (Hep-G2) Cell Line and HdFn Cell Lines
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A simple physical technique was used in this study to create stable and cost-effective copper oxide (CuO) nanoparticles from pure copper metal using the pulsed laser ablation technique. The synthesis of crystalline CuO nanoparticles was confirmed by various analytical techniques such as particle concentration measurement using atomic absorption spectrometry (AAS), field emission scanning electron microscopy (FE-SEM), the energy dispersive X-ray (EDX), and X-ray diffraction (XRD) to determine the crystal size and identify of the crystal structure of the prepared particles. The main characteristic diffraction peaks of the three samples were consistent. The corresponding 2θ is also consistent, and the cytotoxicity of the nanoparticles was

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Publication Date
Thu Jun 01 2023
Journal Name
Medicinal Chemistry
New Niflumic Acid Derivatives as EGFR Inhibitors: Design, Synthesis, In silico Studies, and Anti-proliferative Assessment
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Background:

1,3,4-oxadizole and pyrazole derivatives are very important scaffolds for medicinal chemistry. A literature survey revealed that they possess a wide spectrum of biological activities including anti-inflammatory and antitumor effects.

Objectives:

To describe the synthesis and evaluation of two classes of new niflumic acid (NF) derivatives, the 1,3,4-oxadizole derivatives (compounds 3 and (4A-E) and pyrazole derivatives (compounds 5 and 6), as EGFR tyrosine kinase inhibitors in silico and in vitro.

Methods:

The designed compounds were synthesized using convent

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Effect of Some Materials on Productivity and Activity of -Hemolysin which Produced by Methicillin Ressistant Staphylococcus Aureus
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In this study 77 human isolates of Staphylococcus aureus were obtain from different clinical sources. The results showed that the number of isolates producing α-hemolysin was 32 isolates (41.55%) , while non- hemolysin producing was 45 isolates (58.45%) .The minimum inhibitory concentration (MIC) of methicillin which were 32 micrograms \ ml. The effect of CD-Cholesterol ,Cholesterol ,Cyclodextran(CD) ,Methicillin and Phosphate Buffer Saline(PBS) on α- hemolysin activity was study and the hemolytic Titer was:8 ، 32.768 ,65.536 ,
140.737.488.355.32 , 4.961408E + 25 respectively, while the effect of the same effect with Titer 67.108.864 and low Titer with PBS 3.96140812E + 25. The α – hemolysin toxin was partialy purified by ammoni

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Publication Date
Sun May 10 2020
Journal Name
Baghdad Science Journal
Characterization of Mannitol Fermenter and Salt Tolerant Staphylococci from Breast Tumor Biopsies of Iraqi Women
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The emergence of staphylococci, either coagulase negative (CNS) or coagulase positive (CPS), as important human pathogens has implied that reliable methods for their identification are of large significance in understanding the diseases caused by them. The identification and characterization of staphylococci from biopsies taken from human breast tumors is reported here. Out of 32 tissue biopsies, a total of 12 suspected staphylococci grew on mannitol salt agar (MSA) medium, including 7 fermenters and 5 non-fermenter staphylococci based on traditional laboratory methods. Polymerase chain reaction (PCR) successfully identified seven isolates at the genus level as methicillin resistant Staphylococcus spp. by targeting a common region of the me

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Publication Date
Sun May 10 2020
Journal Name
Baghdad Science Journal
Characterization of Mannitol Fermenter and Salt Tolerant Staphylococci from Breast Tumor Biopsies of Iraqi Women
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         The emergence of staphylococci, either coagulase negative (CNS) or coagulase positive (CPS), as important human pathogens has implied that reliable methods for their identification are of large significance in understanding the diseases caused by them. The identification and characterization of staphylococci from biopsies taken from human breast tumors is reported here. Out of 32 tissue biopsies, a total of 12 suspected staphylococci grew on mannitol salt agar (MSA) medium, including 7 fermenters and 5 non-fermenter staphylococci based on traditional laboratory methods. Polymerase chain reaction (PCR) successfully identified seven isolates at the genus level as methicillin resistant St

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Publication Date
Sat Jun 15 2019
Journal Name
Drug Invention Today
Mucoadhesive oral in situ gel of itraconazole using pH-sensitive polymers: Preparation, and in vitro characterization, release and rheology study
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Publication Date
Tue Aug 01 2023
Journal Name
Iop Conference Series: Earth And Environmental Science
Effect of Light Source and Adenine Sulfate on the Multiplication and Rooting of Kalanchoe blossfeldiana in Vitro
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Abstract<p>This study was conducted in the Tissue Culture laboratory of the Horticultural Department of the Faculty of Agriculture at Karbala University to investigate the effects of a light source (Florescent, LED) and adenine sulfate (Ads) a 0, 40, 80, and 120 mg l-1 on the multiplication and rooting of <italic>Kalanchoe blossfeldiana</italic>. Results showed there are which achieved a 100% response percentage for multiplication and rooting when the addition of adenine sulfate (Ads) under LED light especially a concentration of 120 mg l-1 significantly increased the Shoot height, Shoot number, Number of leaves, Chlorophyll content and fresh, Dry weight. The results showed that they h</p> ... Show More
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Publication Date
Sun Jan 01 2023
Journal Name
Desalination And Water Treatment
The removal of Pb(II) ions from aqueous solutions by immobilized (Chlorophyta) macroalgae: an equilibrium, kinetic, and desorption-regeneration study
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