Cloning and expression of Bacillus subtilis genes in Streptomyces sp.
A local isolate Bacillus subtilis was used, which producing
thennophilic complex enzyme having similar activity of endogluganase
enzyme ( Endo-l,4-B-Dglucanase ).
Partially digested chromosomal DNA of Bacillus subtilis by Eco
Rl restriction enzyme randomly cloned into Eco Rl pSU10l shuttle vector. The resulted hybrid plasmid was transformed into protoplast of
Streptomyces sp. SH-H.
The result revealed that cellulose genes, which expressed into transfonned cells are located on chromosomal DNA fragment having size
4.5·5 kb in size.