Live attenuated vaccine of Strept. Equi was prepared by culturing the bacterium
in increasing concentration of acriflavin and the vaccine was given by
installation in the nose of rabbits. The treated group showed immune response,
the titer of the antibodies was 59.4 ± 4.57 after 2 weeks of vaccination, 237±
18.28 after 4 weeks, 475.4 ± 36.57 after 6 weeks, & reach atiter of 512 ± 0 after
8 weeks, and the treated group did not show any signs after challenge dose,
while the control group died after challenge.