Seventy four (74) samples from cattle, buffalo, goat and sheep were tested to isolate
the FMD virus, 23 isolates of the virus showed Cytopathic Effect (CPE) on the different types
of cell culture.
The CPE appears after 24 hours on fetal lamb kidney (FLK) cells, 48 hours on the fetal
bovine kidney (FBK), 30 hours on primary Fetal Bovine Thyroid cell (BTY) and 20 hours on
the primary fetal ovine thyroid cells (OTY). Solid phase ELISA test showed three serotypes
of FMD virus O, A and Asia1. One step real time RT-PCR for the diagnosis of FMDV by
using 5UTR FMDV primers and taqman probe (SA-IR-219-246F, SA-IR-315-293R and
SAmulti2-P-IR-292-269R). Samples were tested, showed 23 positive machines by using the
Applied Biosystem Fast 7500 real time PCR.