This study was performed to microscopically detect the prevalence of amoebiasis in cattle and sheep fecal samples. One hundred fecal samples were collected from cattle and sheep from 3 completely different area in Baghdad city (Al-Tarmiyah, Al-Fudhaliyah, AbuGhraib). Our data recorded a total amoebiasis infection rate of 54% in cattle and 50% in sheep, without any significant differences between them. Males showed higher infection rates than females in cattle and sheep (62.5%) and (57.14) respectively. The difference between age groups with significant difference (P<0.01) were obtained in ages <1 year (72.7%) and (68.75%) in cattle and sheep respectively. This study has demonstrated the widespread of Entamoeba parasite in cattle and sheep in Baghdad city.