The purpose of this research was to investigate the effect of strength training program on blood lysine and valine levels in tennis players. 6 players aged 18–20 years were sampled. The training intervention was implemented over 8 weeks, 3 sessions per week. Plasma lysine and valine were determined before and after the training period. Results indicated that the contents of lysine and valine increased evidently following training. The finding also indicated that muscle strength and endurance were significantly improved after the training program. It is possible to infer that the training program was beneficial for lysine and valine levels, muscle strength and tennis endurance in players.