The research aims to prepare preventive exercises using different weights to improve some health fitness variables to reduce injury among female students of the College of Physical Education and Sports Sciences for Girls, first stage. The researcher used the experimental method because it is suitable for the nature of the research. The researcher used the experimental design with pre- and post-measures for two groups, one experimental and the other control. The research was conducted on a sample of (65) female students of the first stage of the College of Physical Education and Sports Sciences for Girls, Section (A), Section (B), and Section (C). One of the most important results was that the experimental group outperformed the control group in health fitness variables (strength characterized by speed, motor speed, flexibility, agility, coordination, balance). This is due to the implementation of preventive exercises using the step box with weights for the targeted muscles proposed by the researcher on the experimental group. The experimental group did not achieve a significant increase in bio-kinetic abilities tests (transitional speed, reaction speed) over the control group. One of the most important recommendations was the need to be guided by the content of preventive exercises using different weights in some motor abilities because of what they achieved. Positive impact on first-year female students at the College of Physical Education and Sports Sciences for Girls.