This study was conducted in lath house, Dept. of Horticulture and Landscape Gardening, Coll. of Agricultural Engineering Sciences – Univ. of Baghdad during 2017 / 2018 growing seasons to investigate the influence of biofertilizers and poultry manure on 2 year's old trees of hawthorn transplants. This study included two treatments: four types of biofertilizers control (B₁), Azospirillum brasilense (B₂), Bacillus megatherium (B₃) and A. brasilense + B. megatherium (B₄) and three levels of poultry manure , 0 (M₀), 250g.tree^-1 (M₂₅₀) and 500g.tree^-1 (M₅₀₀) and their interactions. Treatments were replicated four times at factorial experiment in a RCBD. The experimental results showed that biofertilizers treatment (B₄) gave the highest leaves number of 72.42 and 77.83 leaf.plant^-1, highest leaf nitrogen content of 1.62 and 1.75 % and highest leaf IAA content of 34.41 and 38.85 μg g^-1 FW, for both seasons, respectively. Results also showed that poultry manure at levels 500g.tree^-1 (M₅₀₀) was superiority on control treatment and gave the highest leaves chlorophyll content of 59.19 and 59.96 SPAD units and highest leaf GA content of 139.46 and 140.76 μg. g^-1 FW, for both seasons, respectively.