To study the effect of mineral fertilizers (NPK), Organic  and Bio-fertilizers on grain yield of bread wheat(Triticum aestivum L.). A field experiment was conducted during 2014-2015, 2015-2016 at the experimental farm of Field Crop Department, College of Agriculture, University of Baghdad ,Iraq. A split plot arrangement within RCBD, using four replications for two varieties at the main plots  and 14 fertilizer treatments at the sub plots:T₀ (control) ,T₁ (mineral fertilizer as recommended), T₂ (bio-fertilizer), T₃ (organic fertilizer), T₄ (bio+ organic fertilizers), T₅(mineral fertilizer +bio- fertilizer), T₆(50% mineral fertilizer + bio-fertilizer), T₇ (25% mineral fertilizer+ bio-fertilizer), T₈(mineral fertilizer+ organic fertilizer), T₉(50% mineral fertilizer+ organic fertilizer), T₁₀(25% mineral fertilizer+ organic fertilizer), T₁₁ (mineral fertilizer+ organic fertilizer + bio-fertilizer), T₁₂ (50% mineral fertilizer + organic fertilizer + bio-fertilizer), T₁₃(25% mineral fertilizer + organic fertilizer + bio- fertilizer). The results  showes the V. Buhooth 22 was superior in No. of spikes (436.2,474.9) spikem^-2, No. of grains (65.73,70.96) grain spike^-1, grain yield (5.52,5.79) ton h^-1 and biological yield (14.90,15.51) ton h^-1 for both seasons. The Integrated treatment T₁₁ (mineral fertilizer + bio-fertilizer + organic fertilizer) was superior in grain yield (5.71,6.11) ton h^-1,biological yield (15.35,15.92) ton h^-1 for both seasons. It can be concluded a possibility to substitute a part of mineral fertilizers by bio+organic fertilizers.