This study aimed to compare five protocols to optimize elastin purification, where purification protocols for elastin generally result in greatly damaged elastin fibres and this likely influences the biological response. The optimal protocol for elastin purification from common Carp bulbus comprised 4 M of urea solution, pH 7.2 containing 1 % β-mercaptoethanol, washing with distilled water then autoclaving at 121 C° for 15 minutes depending on amino acid analysis, SDS-PAGE electrophoresis, scanning electron microscopy and sulfhydryl content. Then elastin hydrolysate was prepared using partially purified Catfish elastase and the degree of hydrolysis (DH %) and antioxidant activities were recorded, the degree of hydrolysis rapidly increased in the first three hours (reaching 50.43 %) then gradually increasing was shows to reach 65.53 % after 10 hours. Antioxidative properties of elastin hydrolysate were based on three different assays: radical scavenging activity of DPPH, reducing power activity and total antioxidant capacity. The antioxidant activity of elastin hydrolysate increased with increasing the degree of hydrolysis, Where the IC₅₀ of radical scavenging activity of elastin and EC₅₀ of reducing power and total antioxidant capacity reached 2.309, 2.734 and 0.158 mg respectively after 10 hours of hydrolysis.