To improve both the quantity and quality of anise (Pimpinella anisum L.) seeds, a field experiment was conducted in the fall season of 2021-2022 at two locations: Qlyasan Agricultural Research Station and Kanipanka Research Station. The experiment was designed as factorial experiments (2×2×3) within Randomized Complete Block Design (RCBD) using 3 replicates, the first factor was two sowing dates (20^th Oct. and 20^th Nov), the second factor was plant density (50 and 100 plants m^-2), and the third factor was the application of Nitrogen (15 and 30 kg ha^-1 as well as control). The mean results of the both locations indicated that the highest seed yield of 2759 kg ha^-1 gained by the combination of the Oct. 20 sowing date within 100 plants m^-2 density and the application of 30 kg ha^-1 as urea 46% nitrogen, while the highest essential oil yield of 31 kg ha^-1 occurred with the combination of the sowing date of Oct. 20 with 100 plants m^-2 and 15 Kg ha^-1 nitrogen. The Kanipanka location outperformed the Qlyasan location by 91.50% and 46.60% for the characters' seeds yield and essential oil yield, respectively.