This experiment aimed to improve the qualitative traits and stimulate the accumulation of antioxidants  in potato plants under environmentally sustainable agricultural practices. A field experiment was conducted during the spring season of 2023 at the fields of the College of Agricultural Engineering Sciences, University of Baghdad, using a Randomized Complete Block Design (RCBD) within  split-plot arrangement with two factors and three replications (2 × 6 × 3). The first factor involved the application of titanium dioxide nanoparticles (TiO₂-NPs) at concentrations of 0 and 10 ml·L⁻¹, denoted as (T₀) and (T₁), respectively. The second factor included six treatments as follows: natural irrigation (I), prolonged irrigation (D), biofertilizers under prolonged irrigation (D_B), biofertilizers under prolonged irrigation with mannitol (D_BM), biofertilizers under prolonged irrigation with xanthan gum (D_BZ), and biofertilizers under prolonged irrigation with both mannitol and xanthan gum (D_BMZ). The results showed that foliar application of TiO₂-NPs (T₁) led to significant improvements in most of the studied traits. Moreover, the interaction treatment (T₁D_BMZ) recorded the highest qualitative and antioxidant traits compared to the prolonged irrigation treatment without any additions (T₀D).