Barley is the second cereal crop grown after wheat in the Kurdistan region of Iraq. Low rainfall and high-temperature impact barley yields. A study investigating an F₃ population of 100 segregated lines derived from a cross between Clipper, an Australian cultivar known for its high yield, and Local black, a cultivar recognized for its stress tolerance. The segregated F₃ lines were assessed under two different environmental conditions at the Grdarash research station in Erbil and the Qlyasan research station in Sulaimani. The field trial data for the F₃ population experienced analysis of variance and normality tests. Direct selection of high-yielding genotypes is challenging due to the complexity of the trait. Despite significant differences in yield among genotypes from various sources, genotypes showed no significant differences in harvest index (HI%). Location had a greater impact on yield-related traits of the segregated individuals, with temperature being a major influencing factor In Erbil, biological yield (BY) was significantly correlated with yield and its components, except for 1000-grain weight. In Sulaimani, BY was also significantly associated with yield and components, negatively correlated to HI%. Genetic effects were significant for BY but not HI%, with a negative genetic correlation between BY and HI% in Sulaimani. Significant impacts were observed on all attributes using regression analysis. except for 1000-grain weight, which had no significant correlation with BY or other traits. Biplot analysis indicated significant positive correlations between BY and yield-related traits, except for a negative %HI but insignificant correlation with 1000-grain weight.