This experiment was conducted to study the effect of five concentrations of growth regulator of (0, 25 GA3, 50 GA3, 100 Salicylic acid, 150 Salicylic acid) mg.L^-1 and three types of preservative solutions on the vase life and water relations for Gladiolus hybrida L. after cut flower. The experiment was conducted as a factorial experiment (3×5) according to Randomized Complete Blocks Design, with three replicates. The results showed that GA3 (50 mg.L^-1) significantly excelled in relative fresh weight of 155.33%, absorbed water 59.00 g /flower /day, the lost water 32.99 g /flower /day, water balance 26.01 g /flower /day, vase life 16.96 days, dry weight of flowers 22.89 g, and carbohydrate content in petals 22.34 %, and total carotenoids content in petals 7.84 mg/100 g dry weight. The second solution (5% sucrose + 200 mg.L^-1 8.HQS + 200 mg.L^-1citric acid) is significantly excelled in most studied traits. The interaction treatment that consisted of (50 mg.L^-1 + second solution) is significantly excelled in traits of the relative fresh weight 168.47%, absorbed water 66.32 g/flower /day, the lost water 23.39 g/flower /day, water balance 42.93 g/flower /day, vase life 21.25 days, dry weight of flower 26.11 g, carbohydrate content in petals 20.78% and total carotenoids content in petals 10.06 mg/100g dry weight.