Alburnus mossulensis has been exposed to lead and cadmium for (96)h in order to determination lethal concentration 50 (LC₅₀) value. A total of 140 fish used in this experiment divided into two groups each group contain 70 fishes .The first group exposure to pbcl₂ while the second group exposure to cdcl₂. Method of Finney Probit Analysis used to analysis data. LC₅₀  value for PbCl₂ and CdCl₂ was (57.8 and 29.6) mg/l respectively. Then used about 200  fishes divided into 4 groups, the first group exposure to 1/2LC₅₀  of PbCl₂ ,the second group exposure to 1/2LC₅₀ CdCl₂ while the third groups contained (1/2LC₅₀ PbCl₂+1/2LC₅₀ of CdCl₂) as so as control group for peroids (1,4,7 and 14) days to determination level of antioxidants Glutathione (GSH) and the level of lipid peroxidation (Malondialdehyde MDA) . The testes were carried out as three replications, lead ,cadmium and their mixture induced  oxidative stress . The results of this study  explained significant decrease of the level Glutathione and significant increase (Tukey test, P˂0.05)  of the level of Malondialdehye compared with control in all treatments .  It is possible to conclude that lead and Cadmium have cytotoxic effects, Fish mortality rates increase with increasing concentrations used and increasing exposure periods.