This research was aimed to investigate the prevalence of enterotoxin type A in (82) Staphylococcus aureus isolates from different sources(Pathogenic, Soil, Food, Tape water& Nasal swabs from Normal source), the results had shown the capacity of (58) isolates (70.73%) to produce the enterotoxin by using culture method (in solid and liquid medium), concerning the liquid medium, the results conducted that there was a significant differences at P£ 0.05,  among bacterial isolates in the amount of produced enterotoxin in terms of optical density. By using serological ELISA  specific kit, the results had confirmed the ability of (47) bacterial isolates(57.32%)  to produce enterotoxin type A with concentrations were ranged between( 3.7 - 15.7) ng/ml, which represents (81%) of all isolates producing enterotoxins, also the results indicated that there were isolates from different isolation sources can produce this toxin , and the prevalence of enterotoxin type A producing isolates was in pathogenic isolates(43.9%), soil (66.7%), food(92.3%), Tape water(55%) & Nasal swabs(80%), This prevalence of enterotoxin type A which is the most dangerous of Staphylococcal enterotoxins in studied  isolates reflects the risk and healthy importance of this bacteria, regardless of the source of its isolation.