Background: Curve of Spee (CS) is an anteroposterior anatomical curve established by the occlusal alignment of the teeth viewed in the sagittal plane. This occlusal curvature has clinical importance in orthodontics and other fields of dentistry. This study aimed to evaluate the relationship between the CS and dentofacial morphology of different skeletal patterns in both genders. Materials and Methods: Eighty six Iraqi Arab subjects (44females,42 males ) their age ranged from 17 -30 years, classified into: Skeletal I with normal occlusion(15 females and 15 males), skeletal II with CI II div 1 malocclusion (15 females and 15 males) and skeletal III with CI III malocclusion (14 females and 12 males). Forty one variables measured using direct dental cast measurements , dental cast photographs and cephalometric radiographs with the aid of AutoCAD program version 15 (2006). Results: No significant differences in the CS depth between males and females or between right and left sides in both arches of different skeletal patterns. No significant differences in the maxillary CS among the 3 skeletal patterns, the mandibular CS in CI II div 1 malocclusion was larger than normal occlusion and CI III malocclusion. Maxillary CS significantly correlated to arch length, inter canine distance and inter second premolar distance in normal occlusion and overbite in Cl III malocclusion. Mandibular CS significantly correlated with overbite and overjet in Cl II div 1 and Cl III malocclusions. Conclusions: CS was not influence by sides and gender in both arches of different skeletal patterns. CS was concave in the mandibular arch with the maximum concavity at the mesio-buccal cusp tip of the mandibular first molar and convex in the maxillary arch with the maximum convexity at the buccal cusp tip of the maxillary second premolar, in different skeletal patterns. Key words: Curve of Spee, arch length, overbite, overjet, dentofacial morphology.