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Detection of Human Parvovirus B19 antibodies in Pregnant Women with Spontaneous Abortion

Background: Viral infection in pregnancy is known to cause many problems such as fetal malformation; growth restriction and even fetal death; the relationship between viral infection and abortions is not well understood. The infection during pregnancy can affect the fetus due to lack of mother’s immunity.
Objective: To assess the frequency of human parvovirus B19-specific antibody type IgM and IgG among pregnant women with spontaneous abortion.
Patients and Methods: A semi-quantitative enzyme linked immunosorbent assay technique was applied for detection of anti-human parvovirus B19 IgM (Ridascreen-K6031-Germany) and IgG (Ridascreen -K6021- Germany) in sera of 90 pregnant women who have previously experienced abortion were collected from Al-Batool teaching hospital for maternity and children in Baquba-Diyala province, Iraq, during the period from October 2014 till May 2015. The age was ranged from 18-40 years. Statistical analysis was performed by using statistical package of social sciences, version-16, and P-value of < 0.05 as significant difference.
Results: Among study group the mean age was 26.57 years, with an age range 18-40 years. Seropositivity rate was 11.11% (10 cases) for an individual occurrence of human parvovirus B19 IgM alone and 13.33% (12 cases) for a similar individual rate of human parvovirus B19 IgG, while 6 cases (6.66%) had both types of antibodies. Multiple variables regarding occurrence of anti-human parvovirus B 19 specific IgM and IgG antibody simultaneously, was evaluated, and none the variables had significant difference.
Conclusion: The fact that 31.11% of studied participants had specific Parvovirus B19 antibody revealed a high occurrence of primary acute parvovirus infection and non-immune against parvovirus B19. Considering the potential role of this virus in abortion and fetal death, it emphasizes the importance of Parvovirus infection in pregnant women also this finding is comparable with that in the other countries.

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Publication Date
Thu Jan 02 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Seroprevalence of anti- C. trachomatis IgG and IgM antibodies Among Pregnant women in Diyala province

Background: Chlamydia trachomatis infections are the most prevalent bacterial sexually transmitted infections (STI) recognized throughout the world. Screening programs for C. trachomatis is of paramount importance in the prevention of long-term sequelae.

Subjects and methods: A total of 91 normal healthy women were included in this study, which was conducted for the period from 1st. November/ 2011 to 1st. March 2012. They were chosen by simple random selection from Al-Batool Teaching Hospital for maternity and children, and some health care centers in Baquba city. The age range was 17-42 years. 49 (53.8%) and 42 (46.1%) were from rural and urban areas respec

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Publication Date
Wed Aug 31 2022
Journal Name
Al-kindy College Medical Journal
Detection of Parvovirus B19 DNA in pregnant Sudanese women attending The Military hospital using Nested PCR technique : Detection of Parvovirus B19 DNA in pregnant Sudanese women

Background: Parvovirus B19 is a human pathogenic virus associated with a wide range of clinical conditions. During pregnancy congenital infection with parvovirus B19 can be associated with poor outcome, including miscarriage, fetal anemia and non-immune hydrops.  

Objective: The study aimed to determine the prevalenceof Parvovirus B19 DNA in pregnant women attending the Military hospital in Khartoum, demonstrating the association between the virus and poor pregnancy outcomes.

Subjects and methods: This study was a cross sectional study, testing pregnant Sudanese women whole blood samples (n= 97) for the presence of Parvovirus B1

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Publication Date
Fri Jan 01 2016
Journal Name
World Journal Of Experimental Biosciences
Detection of anti-Helicobacter pylori antibodies in sera of women with recurrent spontaneous abortion

To determine the relationship between Helicobacter pylori infection and reproduction disorder (recurrent spontaneous abortion), twenty women patients who undergo spontaneous abortion during first trimester of pregnancy (20-38) years and have been investigated from 2015/12/1 -2016/3/1 and compared to fifteen healthy individuals. All subjects were carried out to measure anti-H. pylori IgA and anti- H. pylori IgG antibodies by enzyme linked immunosorbent assay (ELISA). There was significant elevation (p≤ 0.05) in concentration of anti- H. pylori IgG Abs (6.30± 0.99) compared to control group (4.48± 0.61) and IgA Abs (5.42 ± 0.90 U /ml) as compared to control group (3.92 ± 0.41 U/ml). The percentage of H. pylori IgG and IgA was 20% and 25

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Publication Date
Sun Jan 04 2009
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Human Cytomegalovirus Up-regulates Nuclear Factor-κB in Women with Spontaneous Abortion

Background: Human cytomegalovirus (HCMV) infection during pregnancy causing serious problems through induction of viral genes that enhance the expression of different cellular factors ending in failure of pregnancy.
Patients and Methods: Paraffin embedded sections of curate samples were obtained from 34 women had spontaneous abortion, and 5 women had elective termination of pregnancy (as control), and then subjected for immunohistochemistry analysis to detect human cytomegalovirus (HCMV) early protein, and in situ hybridization technique to detect nuclear factor-κB (NF- κB) mRNA.
Results: Only nine out of 34 women with spontaneous abortion were positive for HCMV early protein, with a significantly higher expression of NF-κB in H

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Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serological and molecular detection of parvovirus b19 in children with acute lymphoblastic leukemia.

Background: Human parvovirus B19 (B19V) is the only human pathogenic parvovirus, It is highly erythrotropic and preferentially replicates in erythroid progenitor cells (EPCs). Recently the effects of B19 infection have been well studied in children with acute lymphoblastic leukemia (ALL).

Objective: To detect parvovirus B19 among children with ALL.

Methods: A cross sectional study involved forty five patients with ALL (21 patients were newly diagnosed ALL and 24 children who underwent chemotherapy) who were attending department of oncology in Children's Welfare Teaching Hospital in Baghdad Medical City between December 2012 and April 2013, compared to forty five of appare

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Publication Date
Mon Jul 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Molecular detection of Epstein Barr Virus in Women with Breast cancer

Background: Epstein Barr Virus (EBV) infection has been implicated in pathogenesis of several types of carcinomas such as nasopharyngeal carcinoma, gastric cancer and bladder cancer and has recently been associated with breast cancer.
Objective: To evaluate the relations between Epstein Barr virus-encoded small RNA (EBER) and breast cancer.
Methods: Twenty two cases of breast cancer were retrieved from the Al-Kadhimiya Teaching Hospital in Baghdad. Clinical data were analyzed from the medical records and formalin fixed, paraffin embedded tumor tissue were examined by Chromogeneic in situ hybridization (ISH) technique for the detection of EBER.
Results: The expression of EBER in tissues patients with breast cancer in the present

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Publication Date
Sun Apr 02 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Knowledge and attitude of pregnant women towards modes of delivery in an antenatal care clinic in Baghdad

Background: Since 1985 the World Health Organization (WHO) had stated: “There is no justification for any region to have Caesarian Sections (CS) rates higher than 10-15%”. Since then, CSs have become increasingly common in both developed and developing countries. The rate of CS has been reported to be as high as 24.5% and 25.8% among Iraqi women in the years 2009 and 2010 respectively.
Objectives: The study was designed to provide data on the level of existing knowledge, attitudes and preference of modes of delivery, which can be used as a platform to raise knowledge among pregnant women and their partners and thereby empowering women to make informed choices.
Patients and Methods: This cross-sectional study was undertaken in a

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Publication Date
Sun Sep 05 2010
Journal Name
Baghdad Science Journal
Detection of interleukin-6 and interleukin-8 in serum from women with recurrent spontaneous abortion

To investigate the role of IL-6 and IL-8 in the immune-regulatory mechanisms involved in the recurrent spontaneous abortion of the first trimester of pregnancy. Serum level of IL-6 and IL-8 were determined in 25 women of age (20-35) years who had a spontaneous abortion of unknown aetiology during the first trimester of pregnancy .They were compared with the corresponding levels of 20 pregnant and non-pregnant women as control groups .cytokine levels were measured by (ELISA) technique .The women with spontaneous abortion had highly significant (P < 0.01) increased serum level of IL-8 and highly significant (P < 0.01 ) decreased level of IL-6 compared to those with normal pregnant and non-pregnant women. The results of this study ma

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Publication Date
Fri Nov 09 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
The Prevalence of Iron Deficiency Anemia among Pregnant Women in Ibn- Albaldy Hospital

Objectives: To study the prevalence of iron deficiency anemia in pregnant women and to find out the degree of
iron deficiency anemia and to find out any association this has been found between the socio- demographic
characteristics and the degree of iron deficiency anemia.
Methodology: A cross sectional study has been conducted in Ibn-Albaldy hospital in Baghdad among 258
pregnant women attending outpatient clinics during the period from the 1st of March 2011 to fifteenth of
October 2011. Data collection was by using a previously designed questionnaire including socio-demographic
characteristics, obstetrical history. A blood sample has been obtained to estimate the hemoglobin, mean
corpuscular volume, serum iron and

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Publication Date
Tue May 11 2021
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Domestic violence among pregnant women in Baghdad\Iraq 2018

Background: Domestic violence against women is a public health problem that affects more than one third of all women globally. It includes any physical, sexual or emotional abuse imposed upon women within family relationships. Several studies in Iraq demonstrated that domestic violence has been increasing over the past two decades.

Objective: Determine the prevalence of domestic violence against pregnant women and factors associated with it.

Patients and methods: A total of 345 pregnant women were included in a cross- sectional study conducted during the period from July - November 2018. They were selected through multistage random sampling from four Primary Health Care C

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