The aims of this study were to prepare Cryptococcus neoformans killed whole cell antigen and evaluate their effects by using DTH- skin test and the differential white blood cells in local rabbits.
Fourteen animals of both sexes were divided randomly into two groups. The first group (7 animals) was immunized with 1 ml of Cryptococcus neoformans killed whole cell antigen (1x 10 cells/ml), subcutaneously. A booster dose was given after 14 days of the first dose. The second group (7 animals) was considered as control group. Based on results of DTH-skin test, no significant differences (P< 0.05) were recorded between the concentrations, 15 mg/ml and 7.5 mg/ml after 24, 48 and 72 hrs., but there was a significant differences (P<0.05) between these concentrations and 7.5 mg/ml and 3.75 mg/ml and control site. There was no significant difference between all types of cells (neutrophils, lymphocytes, monocytes, and eosinophils) of immunized and control groups.
The aim of the study was molecular detection of C. neoformans that isolated from 150 (88 female and 62 male) clinical samples (sputum samples) from pulmonary patients in Baghdad. The diagnoses of Cryptococcus neoformans in samples was done by using direct microscopic examination, culture media and PCR Technology. Microscopic examination and cultured revealed that 65 out of 150 (43.33 %) samples were positive and the others samples were Negative. Results of the genetic diagnosis looking for the fungi causing cryptococcosis using primers specific for ITS gene which were specially designed for this study revealed that 6 (4 %) of sputum samples were positive. In this study used the PCR technology due to the present
... Show MoreBackground: wound infections are associated with increased morbidity and mortality. Etiologic agents of wound infections vary with geographical location
Patients and Methods: All burn patients admitted to Specialized Burn Hospital from November 2011 to May 2012. Once fungal infection was suspected clinically, swabs were harvested for the culture of yeast. The sensitivities of the identified yeast were determined and the positive samples and cases were analyzed.Objective: This study presents Cryptococcus neoformans and their Characteristics identified from burn patients at a major Iraqi Specialized Burn Hospital.
Result: The most predominant yeast
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A streptococci has recognized as Streptococcus spp., associate with acute pharyngitis. S. pyogenes infection Has detected in the Hospital and Health Center in Tikrit city. Throat swabs sample has obtained and cultured on a sheep blood agar plate. Identification of S. pyogenes was performed by using the VITEK 2 automatic system. It detected of 50 samples from children included 25 were positive for S. pyogenes infection. were aged 10-35 years old and included 30 male and 20 female. . While 25 sample Negative of S. pyogenes infection (The control group included 25 clinically healthy children without S. pyogenes infection matched for age and sex with cases pateints). No significa
... Show MoreThis study was conducted to know some Immune responses in women with vaginal candidias is by Candidia species. Hemoglubin (Hb) was decreased significantly in patient women comparing with control group. The differential count of Blood cells revealed that neutrophils and monocytcs were increased significantly in patient women comparing with control group. The percentage of the Phagocytosis index was decreased significantly in patient women (66.45 + 15.05) comparing with control group (73.72 + 3.77) and the T- lymphocytes were decreased also in patient women (57.75 + 18.787) comparing with control group (74.25 + 7.759).
A total of 33 Iraq male positive for Toxoplasmosis and Iraq male negative for Toxoplasmosis (controls) were studies to Evaluation of some biochemical and immunological parameters changes.The parameters included lipid profile such as (Cholesterol(C), Triglycerides(TG), High-Density Lipoprotein (HDL), Low-Density Lipoprotein (LDL) and very Low-Density Lipoprotein (VLDL) and complement component C3 and C4. The results revealed significant decrease in the total cholesterol, Triglycerides, LDL and non-significant in vLDL (129.96±1.63, 130.69± 2.80, 87.19±1.97, 29.24± 0.83 mg/dl respectively) and non-significant increase in HDL(24.22 ±0.62) mg/dl compared with control group(152.07± 1.63, 156.48± 6.55, 99.26 ±1.39, 31.49± 1.30 and 21.31±
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Psoriasis is a common, chronic, immune-mediated skin disease with systemic pro-inflammatory activation. This study was designed to estimate the level of two cytokines, Interleukin-36 (IL-36) and Interleukin-10 (IL-10), in psoriasis female patients. The study was accomplished on 50 Iraqi patients with psoriasis who were referred to the consulting clinic at Al-Yarmouk Teaching Hospital during the period from November 2018 to March 2019. These patients were diagnosed under the supervision of dermatologists. For the purpose of comparison, the study included 30 healthy women as a healthy control group. The serum levels of cytokines were measured using the enzyme-linked immunosorbent technique (ELISA).The results of this study show
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Background: Complement (C) & CRP in patients with Rheumatoid arthritis (RA)'could be trigger disease activity.
Aim: To study the correlation between C, CRP, IgM,, IgG, IgA & some clinical presentation in RA patients.
Methods: Latex agglutination test(AG) & single radial immunoassay(SRIA) were used to asses CRP, IgMJgGJgA,, C, in 74 patients with RA.
Results: IgA & IgG were significantly increased, while the mean ofC3, C4 were slightly elevated in RA patients .
Conclusion: There is correlation between IgG and IgM with joint deformity and joint swelling respectively, while C3 was showed statistically significant P<0.01 with joint
stiffness, joint swelling and Rheumatoid nodule, whe
The present study was performed to evaluate the level of some risk factors (biochemical and immunological) in hypothyroid Iraqi patients considering the different thyroid functional states (hypothyroidism and subclinical hypothyroidism).The study includes 82 patients clinically diagnosed with hypothyroidism. Three study groups have been investigated: (47 clinical hypothyroid patients, 12 subclinical hypothyroid patients 23 healthy individuals) of different ages. This study, show that the proportion of females (83.3 %), (87.2%) in subclinical and clinical hypothyroidisim respectively higher than the proportion of males (16.7%),(12.8%) in subclinical and clinical hypothyrodism respectively of the total patients.The majority of subclinical hyp
... Show MoreThis study was designed to investigate the effect of the Clenbuterol drug in some physiological parameters of Liver, Kidney function and lipidprofie concentration. Fourty adult male rabbits were used in this study. The animals were randomly divided into four groups,the first group was given distilled water (control) ,while the other third groups were given (2,4,6 µg/kg) of Clenbuterol drug for 30 days. Blood samples were collected after 14 and 30 days of the treatment for biochemical study. Drug caused non significant increase(P<0.05)in the AST and ALT enzymeactivity in second and third groups during treatment period ,while we note a significant increase(P<0.05) in both AST,ALT enzyme level in fourth group compared with control group , as
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The local study were selected 70 Iraqi Arab Patients (39 females and 31 males), who referred to HLA laboratory in teaching laboratories in Baghdad medical city from April 2009 – May 2010 were diagnosed with Behcete's disease, C-reactive protein concentration(CRP) was evaluated by ELISA technique, anti-nuclear antibody (ANA) and anti-neutrophil cytoplasmic antibody (ANCA) were evaluated by Indirect immunofluorescence technology. The result showed significant differences (P<0.05) of CRP concentration in patients group compared with control, negative antinuclear nuclear antibody in all cases while ANCA was positive in 8.5% of patients with type P (PANCA). These results lead to suggest that ANCA auto-antibody may have a role in triggeri
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