Laportea aestuans L. (Chew), Commelina virginica (L.) and Sida rhombifolia (L.) are common wild plants used in treating several ailments including diarrhea, dysentery, hernia, oedema, ulcers and many more in traditional African medicine especially, Nigeria. The potentials of Random Amplified polymorphic DNA (RAPD) primers in delimiting intra-specific variation in L. aestuans, C. virginica and S. rhombifolia was assessed using three RAPD primers. Plant and soil samples were collected from 19 local government areas in Lagos State and assessed for genetic and biochemical relationships. A total of 56 bands were produced of which 44 were polymorphic. Maximum number of bands (21) was produced by OPY20 while OPC04 produced minimum number of bands (14). The maximum and minimum percentage polymorphisms were 100% (OPC04) and 50% (OPA12), The dendrogram of genetic diversity had a genetic distance range of 0.57 to 1.00, 0.58 to 1.00 and 0.52 to 1.00 and clustered at 0.57, 0.58, 0.52 implying 57%, 58%, 52% similarity and 43%, 42% and 48% variability for L. aestuans, C. virginica and S. rhombifolia respectively. Results of phytochemical analysis show that S. rhombifolia is particularly high in steroids (I134.19±0.23), flavonoids (118.65±0.18), phenolics (125.25±1.43), alkaloids (63.56±0.11) and triterpenes (72.44±0.12) but low in saponins (23.29±0.05), glycosides (6.79±0.01) and tannins (7.10±0.02).
The purpose of this paper is to study a new types of compactness in the dual bitopological spaces. We shall introduce the concepts of L-pre- compactness and L-semi-P- compactness .
The purpose of this paper is to study new types of open sets in bitopological spaces. We shall introduce the concepts of L- pre-open and L-semi-p-open sets
Chemical analysis for evaluation of Nigella sativa L. (black cumin) seeds showed a composition of Fat 39% ; Protein 28% ; Carbohydrate 21% ; Moisture 6% and Ash 4.5% . It was found that the black seed contains the following mineral element : Magnesium 0.26 gm /100gm seed ; Calcium 0.25 gm /100gm seed and Iron 25 ?g / gm /100gm seed ; zinc 4.51?g /gm /100gm seed and Copper 3.60 ?g /gm /100gm seed. The analysis also showed that mineral element I. e. ; lead ; Cobalt ; Nickel ; Chrom ; Cadmium and Aresenic are not present . It was found that the fat of the black seed contains the following fatty acids : Myristic 2.8%; Palmtic 16.6%; Stearic 0.8 % ; Oleic 13.79% ; Linoleic 64.2% and Arachidic 1.9% .
Leishmania causes disease ranging from self-healing cutaneous to fatal visceral leishmaniasis (VL). Leishmaniasis is reported endemic in 88 countries, including Iraq, in which 82% in low-income countries. The diseases develop following the bite of sand flies injecting Leishmania promastigotes into skin. Promastigotes transform into amastigotes in vivo multiplying within macrophages. In this study we have investigated the ability of axenic procyclic promastigotes of cutaneous Leishmania tropica and visceral Leishmania donovani survive in M199 media with or without Fetal Bovine Serum (FBS) added. Three time incubation periods were adopted (24, 48 and 72) hours and the results showed that L. tropica was able to survive and multiply in both
... Show MoreThis study aimed to test the effect of using different concentrations of three different plants extracts to inhibit the growth of gram negative and gram positive bacteria by two technics. Eucalyptus camaldulensis bark, Glycyrrhiza glabra rhizomes and Morus nigra leaves ethanolic extract at (0,20,30,40 and 50 mg/ml) were used. The antimicrobial activity and the biofilm inhibition assay used with these extracts showed positive effect in inhibiting the growth of bacteria. E.amaldulensis extract showed the higher effect than G. glabra and M.nigra extracts in antimicrobial activity assay, while the effect of E. camaldulensis extract in biofilm inhibition assay was higher than G. glabra that was higher than M. nigra extracts for both gram nega
... Show MoreThe paper comprise comparative palynological study of six species belong to the genera Urtica L. and Parietaria L.(P.alsinifolia Del., P.lusitanica L., P.judaicaL., U.urens L., U.dioica L., and U.pilulifera L.) of the Family Urticaceae in Iraq. All pollen grains were small size and found to be porate, Stephanoporate, Zonoporate. Characters such as shape of pollen grain, number and Dimention of pores, and Sculpturing of pollen grains were overlapped between species and of limited taxonomic value.
Total flavonoid contents, reductive ability and radical scavenging activity were studied in the methanol extracts of Arum maculatum L. and Physalis peruviana L. The results revealed that A. maculatum extract had total flavonoids of 535.3±109.9 µg/ml, which was significantly higher than the recorded flavonoids in P. peruviana extract (352.0±12.7 µg/ml). Assessment of reductive ability revealed that both extract were effective in such activity and concentration-dependent. The highest absorbance was found at the concentration 0.64 mg/ml of A. maculatum methanol extract (0.929±0.006), which was significantly higher (P ≤ 0.05) than the corresponding concentration of P. peruviana extract (0.850
... Show MoreTrimmed Linear moments (TL-moments) are natural generalization of L-moments that do not require the mean of the underlying distribution to exist. It is known that the sample TL-moments is unbiased estimators to corresponding population TL-moment. Since different choices for the amount of trimming give different values of the estimators it is important to choose the estimator that has minimum mean squares error than others. Therefore, we derive an optimal choice for the amount of trimming from known distributions based on the minimum errors between the estimators. Moreover, we study simulation-based approach to choose an optimal amount of trimming and maximum like hood method by computing the estimators and mean squares error for range of
... Show MoreThis study observed the genetic diversity and relationships among 4 species belonging to genus Gladiolus L. , by using the Random Amplified Polymorphic DNA (RAPD) technique , the study includes extraction of genomic DNA from the dray leaves by using commercial kit . 4 random primers used the produced of many polymorphic bands among the 4 species , it was also possible to fined the DNA fingerprint of all studied species.Through the appearance of a number of bands that were unique to each species.Genetic distances ranged from 0.10 to 0.86, and used cluster analysis were performed to construct dendrogram.Cluster analysis grouped the 4 species Into tow main clusters depending on their ancestor and their morphological
... Show MoreThe present research was performed to study the qualitative and quantitative composition of epiphytic algae on the aquatic host plant Ceratophyllum demersum L. Four sites in Tigris River, at Wassit Governorate were covered, during the seasons of Autumn 2017, winter 2018, Spring 2018, and Summer 2018. The study also included measuring the physiochemical parameters (temperature of air and water, pH , water level, EC, salinity, TDS, TSS, dissolved oxygen, BOD5, alkalinity, total hardness, calcium, magnesium, total nitrogen, total phosphourus). The total number of species of epiphytic algae was145 species, 98 species belonging to Bacillariophyceae, followed by 27species of class Cyanophyceae, 19 species of class Chloroph
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